Butyrate Induced Cell Cycle Arrest in Bovine Cells through Targeting Gene Expression Relevant to DNA Replication Apparatus

Using real-time RT-PCR and Western blot analysis in bovine kidney epithelial cells, we systematically investigated the effects of butyrate on patterns of gene expression relevant to DNA replication apparatus. The real-time PCR and Western blot data generally confirmed previously reported microarray data. Of the five genes tested by quantitative RT-PCR, CDKN1A (p21waf1) was up regulated, CDC2/cdk1, MCM6, ORC1L were down regulated, while ORC3L expression remained unchanged following butyrate treatment. Also consistent with RT-PCR results, Western blot analysis confirmed that butyrate up-regulated cyclin-kinase inhibitor p21waf1 in a does-dependent manner. In contrast, butyrate treatment had no effect on the expression of ERK 1/2 proteins. Also consistent with mRNA results, ORC1 and MCM3 proteins were down-regulated by butyrate treatment, while ORC2 protein remained unchanged. The present results suggest that ORC1, not ORC2 or ORC3, along with MCM proteins play a critical role in regulating the initiation of DNA replication and cell cycle progression in MDBK cells and are targets of butyrate regulation.

• Publication year

  2008

• Venue

  Gene Regulation and Systems Biology

• Publication date

  2008-03-17

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.4137/GRSB.S465PMID 19787080PMCID 2733093
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Transcriptional Response Of E. coli Upon FimH-mediated Fimbrial Adhesion

  2010cited by this paper
• Differential regulation of the tyrosine hydroxylase and enkephalin neuropeptide transmitter genes in rat PC12 cells by short chain fatty acids: concentration-dependent effects on transcription and RNA stability.

  2007cited by this paper
• Pathway analysis identifies perturbation of genetic networks induced by butyrate in a bovine kidney epithelial cell line

  2007cited by this paper
• Histone deacetylases as targets for dietary cancer preventive agents: lessons learned with butyrate, diallyl disulfide, and sulforaphane.

  2006cited by this paper
• Regulation of Replication Licensing by Acetyltransferase Hbo1

  2006cited by this paper
• Butyrate induces profound changes in gene expression related to multiple signal pathways in bovine kidney epithelial cells

  2006influential reference
• Dietary agents as histone deacetylase inhibitors

  2006cited by this paper
• Butyrate-induced apoptosis and cell cycle arrest in bovine kidney epithelial cells: involvement of caspase and proteasome pathways.

  2005influential reference
• Role for Cdk1 (Cdc2)/Cyclin A in Preventing the Mammalian Origin Recognition Complex's Largest Subunit (Orc1) from Binding to Chromatin during Mitosis

  2004cited by this paper
• The ORC1 Cycle in Human Cells

  2003cited by this paper
• The ORC1 Cycle in Human Cells

  2003cited by this paper
• DNA replication in eukaryotic cells.

  2002cited by this paper
• Xenopus Mcm10 binds to origins of DNA replication after Mcm2-7 and stimulates origin binding of Cdc45.

  2002cited by this paper
• Nuclear Organization of DNA Replication Initiation Proteins in Mammalian Cells*

  2002cited by this paper
• Human origin recognition complex large subunit is degraded by ubiquitin-mediated proteolysis after initiation of DNA replication.

  2002cited by this paper
• Cell cycle‐dependent regulation of the association between origin recognition proteins and somatic cell chromatin

  2002cited by this paper
• Mammalian Orc1 Protein Is Selectively Released from Chromatin and Ubiquitinated during the S-to-M Transition in the Cell Division Cycle

  2002cited by this paper
• Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

  2001cited by this paper
• Nucleosomes positioned by ORC facilitate the initiation of DNA replication.

  2001cited by this paper
• Association of Human Origin Recognition Complex 1 with Chromatin DNA and Nuclease-resistant Nuclear Structures*

  2000cited by this paper
• Subsets of Human Origin Recognition Complex (ORC) Subunits Are Expressed in Non-proliferating Cells and Associate with Non-ORC Proteins*

  2000cited by this paper
• Butyrate inhibits colon carcinoma cell growth through two distinct pathways.

  1998cited by this paper
• The chromatin structure of Saccharomyces cerevisiae autonomously replicating sequences changes during the cell division cycle

  1991cited by this paper
• Butyrate blocks the accumulation of CDC2 mRNA in late G1 phase but inhibits both the early and late G1 progression in chemically transformed mouse fibroblasts BP‐A31

  1990cited by this paper
• Energy contributions of volatile fatty acids from the gastrointestinal tract in various species.

  1990cited by this paper
• Nucleosome positioning can affect the function of a cis-acting DMA elementin vivo

  1990cited by this paper
• n-Butyrate causes histone modification in HeLa and Friend erythroleukaemia cells

  1977cited by this paper
• Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2 2 DD C T Method

  year unknowncited by this paper

• Transcript and blood-microbiome analysis towards a blood diagnostic tool for goats affected by Haemonchus contortus

  2022cites this paper
• Functional genome and microbiome in blood of goats affected by the gastrointestinal pathogen Haemonchus contortus

  2021cites this paper
• Establishment and transcriptomic analyses of a cattle rumen epithelial primary cells (REPC) culture by bulk and single-cell RNA sequencing to elucidate interactions of butyrate and rumen development

  2020cites this paper
• Data of epigenomic profiling of histone marks and CTCF binding sites in bovine rumen epithelial primary cells before and after butyrate treatment

  2019cites this paper
• Origin recognition complex subunit 1 regulates cell growth and metastasis in glioma by altering activation of ERK and JNK signaling pathway.

  2019cites this paper
• Temporal dynamics in meta longitudinal RNA-Seq data

  2019cites this paper
• Transcriptomic Impacts of Rumen Epithelium Induced by Butyrate Infusion in Dairy Cattle in Dry Period

  2018cites this paper
• Differential human gut microbiome assemblages during soil-transmitted helminth infections in Indonesia and Liberia

  2018cites this paper
• Transcriptome analysis revealed anti-obesity effects of the Sodium Alginate in high-fat diet -induced obese mice.

  2018cites this paper
• Polymannuronic acid ameliorated obesity and inflammation associated with a high-fat and high-sucrose diet by modulating the gut microbiome in a murine model.

  2017cites this paper
• Chondroitin sulfate disaccharides modified the structure and function of the murine gut microbiome under healthy and stressed conditions

  2017cites this paper
• The effect of helminth infection on the microbial composition and structure of the caprine abomasal microbiome

  2016cites this paper
• The Histone‐Deacetylase‐Inhibitor Suberoylanilide Hydroxamic Acid Promotes Dental Pulp Repair Mechanisms Through Modulation of Matrix Metalloproteinase‐13 Activity

  2016cites this paper
• Effect of roughage on rumen microbiota composition in the efficient feed converter and sturdy Indian Jaffrabadi buffalo (Bubalus bubalis)

  2015cites this paper
• Bioinformatic Dissecting of TP53 Regulation Pathway Underlying Butyrate-induced Histone Modification in Epigenetic Regulation

  2014cites this paper
• Characterization of the rumen microbiome of Indian Kankrej cattle (Bos indicus) adapted to different forage diet

  2014cites this paper
• Inhibition of azoxymethane-induced preneoplastic lesions in the rat colon by a stearic acid complexed high-amylose cornstarch using different cooking methods and assessing potential gene targets

  2014cites this paper
• Quantification of Transcriptome Responses of the Rumen Epithelium to Butyrate Infusion using RNA-seq Technology

  2012cites this paper
• Assessing the inhibition of azoxymethane-induced preneoplastic lesions in the rat colon by cooked resistant corn starches and identifying potential genetic targets

  2012cites this paper
• Genome-wide ChIP-seq mapping and analysis reveal butyrate-induced acetylation of H3K9 and H3K27 correlated with transcription activity in bovine cells

  2012cites this paper
• Alternative Splicing Regulated by Butyrate in Bovine Epithelial Cells

  2012cites this paper
• Perturbation Dynamics of the Rumen Microbiota in Response to Exogenous Butyrate

  2012cites this paper
• The regulation of Gb 3 biosynthesis in cancer cells : Implications for membrane dynamics

  2011cites this paper
• MicroRNA (miRNA) Expression is Regulated by Butyrate-Induced Epigenetic Modulation of Gene Expression in Bovine Cells

  2010cites this paper