Quantitation of putative activator‐target affinities predicts transcriptional activating potentials.

No abstract is available for this paper.

• Publication year

  1996

• Venue

  EMBO Journal

• Publication date

  1996-08-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1002/j.1460-2075.1996.tb00769.xPMID 8670900PMCID PMC452115
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Modulation of promoter occupancy by cooperative DNA binding and activation-domain function is a major determinant of transcriptional regulation by activators in vivo.

  1996cited by this paper
• The ability to associate with activation domains in vitro is not required for the TATA box-binding protein to support activated transcription in vivo.

  1995cited by this paper
• GAL4 interacts with TATA-binding protein and coactivators

  1995cited by this paper
• Mechanism of active transcriptional repression by the retinoblastoma protein

  1995cited by this paper
• Crystal structure of a TFIIB–TBP–TATA-element ternary complex

  1995cited by this paper
• A highly conserved ATPase protein as a mediator between acidic activation domains and the TATA-binding protein

  1995cited by this paper
• Stimulation of RNA polymerase II transcription initiation by recruitment of TBP in vivo

  1995cited by this paper
• p53 transcriptional activation mediated by coactivators TAFII40 and TAFII60.

  1995cited by this paper
• CCAAT/enhancer binding protein‐alpha amino acid motifs with dual TBP and TFIIB binding ability co‐operate to activate transcription in both yeast and mammalian cells.

  1995cited by this paper
• The activation domain of GAL4 protein mediates cooperative promoter binding with general transcription factors in vivo.

  1995cited by this paper
• Contact with a component of the polymerase II holoenzyme suffices for gene activation.

  1995cited by this paper
• Molecular cloning and expression of the 32-kDa subunit of human TFIID reveals interactions with VP16 and TFIIB that mediate transcriptional activation.

  1995cited by this paper
• Structure and function of transcriptional activation domains.

  1995cited by this paper
• Recruiting TATA-binding protein to a promoter: transcriptional activation without an upstream activator

  1995cited by this paper
• Connecting a promoter-bound protein to TBP bypasses the need for a transcriptional activation domain

  1995cited by this paper
• An RNA polymerase II holoenzyme responsive to activators

  1994cited by this paper
• Modulating the potency of an activator in a yeast in vitro transcription system

  1994cited by this paper
• Purification, cloning, and characterization of a human coactivator, PC4, that mediates transcriptional activation of class II genes.

  1994cited by this paper
• Binding of basal transcription factor TFIIH to the acidic activation domains of VP16 and p53

  1994cited by this paper
• Mutational analysis of the transcription activation domain of RelA: identification of a highly synergistic minimal acidic activation module

  1994cited by this paper
• A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II.

  1994cited by this paper
• Genetic evidence that an activation domain of GAL4 does not require acidity and may form a beta sheet.

  1993cited by this paper
• Interaction between an acidic activator and transcription factor TFIIB is required for transcriptional activation

  1993cited by this paper
• A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast.

  1993cited by this paper
• Drosophila TAFII40 interacts with both a VP16 activation domain and the basal transcription factor TFIIB.

  1993cited by this paper
• Genetic evidence that an activation domain of GAL4 does not require acidity and may form a β sheet

  1993cited by this paper
• The acidic activation domains of the GCN4 and GAL4 proteins are not alpha helical but form beta sheets.

  1993cited by this paper
• Determinants of binding-site specificity among yeast C6 zinc cluster proteins.

  1993cited by this paper
• The acidic activation domains of the GCN4 and GAL4 proteins are not α helical but form β sheets

  1993cited by this paper
• Nondissociation of GAL4 and GAL80 in vivo after galactose induction.

  1992cited by this paper
• Yeast and human TFIID with altered DNA-binding specificity for TATA elements.

  1992cited by this paper
• The C-terminal domain of the Escherichia coli DNA gyrase A subunit is a DNA-binding protein.

  1991cited by this paper
• GAL11P: a yeast mutation that potentiates the effect of weak GAL4-derived activators.

  1990cited by this paper
• Use of T7 RNA polymerase to direct expression of cloned genes.

  1990cited by this paper
• Mutations that increase the activity of a transcriptional activator in yeast and mammalian cells.

  1990cited by this paper
• A potent GAL4 derivative activates transcription at a distance in vitro.

  1990cited by this paper
• Direct and selective binding of an acidic transcriptional activation domain to the TATA-box factor TFIID

  1990cited by this paper
• Analysis of Saccharomyces cerevisiae his3 transcription in vitro: biochemical support for multiple mechanisms of transcription

  1990cited by this paper
• GAL4 activates gene expression in mammalian cells.

  1988cited by this paper
• New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites.

  1988cited by this paper
• GAL4 derivatives function alone and synergistically with mammalian activators in vitro.

  1988cited by this paper
• Yeast HAP2 and HAP3: transcriptional activators in a heteromeric complex.

  1988cited by this paper
• How eukaryotic transcriptional activators work

  1988cited by this paper
• Structural and functional characterization of the short acidic transcriptional activation region of yeast GCN4 protein

  1988cited by this paper
• A Method for Gene Disruption That Allows Repeated Use of URA3 Selection in the Construction of Multiply Disrupted Yeast Strains

  1987cited by this paper
• Interaction of positive and negative regulatory proteins in the galactose regulon of yeast.

  1987cited by this paper
• The carboxy-terminal 30 amino acids of GAL4 are recognized by GAL80.

  1987cited by this paper
• Deletion analysis of GAL4 defines two transcriptional activating segments.

  1987cited by this paper
• An adenovirus E1a protein region required for transformation and transcriptional repression.

  1986cited by this paper
• Use of lacZ fusions to delimit regulatory elements of the inducible divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae

  1984cited by this paper
• Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli.

  1983cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper

• Molecular characteristic of yeast Gal80 as a transcriptional factor: A mini-review

  2024cites this paper
• Systems for Targeted Silencing of Gene Expression and Their Application in Plants and Animals

  2024cites this paper
• Grammar rules and exceptions for the language of transcriptional activation domains

  2024cites this paper
• Sequence features of transcriptional activation domains are consistent with the surfactant mechanism of gene activation

  2023cites this paper
• Comparison of GAL80ts and Tet-off GAL80 transgenes

  2023cites this paper
• Zinc cluster transcription factors frequently activate target genes using a non-canonical half-site binding mode

  2023cites this paper
• Transcription factor clusters enable target search but do not contribute to target gene activation

  2022cites this paper
• Transcriptional repressor Gal80 recruits corepressor complex Cyc8–Tup1 to structural genes of the Saccharomyces cerevisiae GAL regulon

  2021cites this paper
• Variation in the modality of a yeast signaling pathway is mediated by a single regulator

  2021cites this paper
• Fluorescence resonance energy transfer in revealing protein-protein interactions in living cells.

  2021cites this paper
• Transcriptional regulatory proteins in central carbon metabolism of Pichia pastoris and Saccharomyces cerevisiae

  2020influential citation
• Quantitative analysis of transcription factor binding and expression using calling cards reporter arrays

  2020cites this paper
• Mediator subunit Med15 dictates the conserved “fuzzy” binding mechanism of yeast transcription activators Gal4 and Gcn4

  2019cites this paper
• Sequence modification of the master regulator Pdr1 interferes with its transcriptional autoregulation and confers altered azole resistance in Candida glabrata

  2018cites this paper
• A Tet/Q Hybrid System for Robust and Versatile Control of Transgene Expression in C. elegans

  2018influential citation
• Evolutionary Transition of GAL Regulatory Circuit from Generalist to Specialist Function in Ascomycetes.

  2018cites this paper
• Regulating the UAS/GAL4 system in adult Drosophila with Tet-off GAL80 transgenes

  2017cites this paper
• Analysis of Gal 4-directed transcription activation using Tra 1 mutants selectively defective for interaction with Gal 4

  2017cites this paper
• The 9aaTAD Is Exclusive Activation Domain in Gal4

  2017cites this paper
• Gal4 activation domain 9aaTAD could be inactivated by adjacent mini-inhibitory domain and reactivated by distal re-activation domain

  2017influential citation
• The 9aaTAD Transactivation Domains: From Gal4 to p53

  2016cites this paper
• A Modified Reverse One-Hybrid Screen Identifies Transcriptional Activation Domains in PHYTOCHROME-INTERACTING FACTOR 3

  2016cites this paper
• Nucleosome distortion as a possible mechanism of transcription activation domain function

  2016cites this paper
• Nucleosome avidities and transcriptional silencing in yeast.

  2015cites this paper
• A purified truncated form of yeast Gal4 expressed in Escherichia coli and used to functionalize poly(lactic acid) nanoparticle surface is transcriptionally active in cellulo.

  2015cites this paper
• New insights for Drosophila GAGA factor in larvae

  2015cites this paper
• A targeted gene expression system using the tryptophan repressor in zebrafish shows no silencing in subsequent generations

  2014influential citation
• Inducible, tightly regulated and growth condition-independent transcription factor in Saccharomyces cerevisiae

  2014cites this paper
• The Chemistry of Regulation of Genes and Other Things

  2014cites this paper
• Solvent-exposed serines in the Gal4 DNA-binding domain are required for promoter occupancy and transcriptional activation in vivo.

  2014influential citation
• Recent progress in metabolic engineering for the production of biofuels and biochemicals from renewable sources with particular emphasis on catabolite regulation and its modulation.

  2013cites this paper
• ERG11-Mediated Azole Resistance in Candida albicans

  2013influential citation
• Kinetic and Conformational Characterization of Transcriptional Activator-Coactivator Interactions.

  2013influential citation
• Analysis of Gal4-directed transcription activation using Tra1 mutants selectively defective for interaction with Gal4

  2012cites this paper
• Genetic targeting of specific neuronal cell types in the cerebral cortex.

  2012cites this paper
• Genetic Approaches to Study Transcriptional Activation and Tumor Suppression: A Dissertation

  2012cites this paper
• Implementation and Optimization of an in vivo Photo-crosslinking Methodology to Define Direct Targets of Transcriptional Activators

  2012cites this paper
• Endogenous gypsy insulators mediate higher order chromatin organization and repress gene expression in Drosophila

  2011influential citation
• Improved galactose fermentation of Saccharomyces cerevisiae through inverse metabolic engineering

  2011cites this paper
• Examination of the Unconventional Role of the 19S Proteasome Subcomplex in RNA Polymerase II Transcription in Saccharomyces cerevisiae

  2011cites this paper
• Transient-state Kinetic Analysis of Transcriptional Activator·DNA Complexes Interacting with a Key Coactivator*

  2011cites this paper
• Interplay of a Ligand Sensor and an Enzyme in Controlling Expression of the Saccharomyces cerevisiae GAL Genes

  2011cites this paper
• Promotor engineering in Saccharomyces cerevisiae for transcriptional control under different physiological conditions

  2011cites this paper
• Rapid GAL Gene Switch of Saccharomyces cerevisiae Depends on Nuclear Gal3, Not Nucleocytoplasmic Trafficking of Gal3 and Gal80

  2011cites this paper
• The GAL genetic switch: visualisation of the interacting proteins by split‐EGFP bimolecular fluorescence complementation

  2011cites this paper
• Isolation of compensatory inhibitor domain mutants to novel activation domain variants using the split‐ubiquitin screen

  2011cites this paper
• Distinct regulatory mechanisms of eukaryotic transcriptional activation by SAGA and TFIID.

  2011cites this paper
• for Induced and Stable Structure Conformation of the Mineralocorticoid Receptor N-terminal Domain: Evidence

  2010cites this paper
• Split-EGFP screens for the detection and localisation of protein-protein interactions in living yeast cells.

  2010cites this paper
• Transcriptional regulation of the endo-polygalacturonase-encoding gene in Saccharomyces cerevisiae

  2010cites this paper
• Inducible gene expression: diverse regulatory mechanisms

  2010cites this paper
• Conformation of the mineralocorticoid receptor N-terminal domain: evidence for induced and stable structure.

  2010cites this paper
• A tryptophan-rich peptide acts as a transcription activation domain

  2010influential citation
• Systems biology of GAL regulon in Saccharomyces cerevisiae

  2010cites this paper
• Transcriptional regulation of carbohydrate metabolism in Candida albicans

  2009cites this paper
• Histone acetylation: truth of consequences?

  2009cites this paper
• No Splicing, No Dicing: Non-proteolytic Roles of the Ubiquitin-Proteasome System in Transcription*

  2009cites this paper
• The disorderly study of ordered recruitment

  2009cites this paper
• Energetic limits to metabolic flexibility: responses of Saccharomyces cerevisiae to glucose-galactose transitions.

  2009cites this paper
• Impact of nonnatural amino acid mutagenesis on the in vivo function and binding modes of a transcriptional activator.

  2009cites this paper
• Mediation of Pleiotropic Drug Resistance by Zinc Cluster Transcriptional Regulators in Saccharomyces cerevisiae

  2009cites this paper
• Dynamic response of Saccharomyces cerevisiae to fermentative growth conditions

  2009cites this paper
• IQ motif selectivity in human IQGAP1: binding of myosin essential light chain and S100B

  2008cites this paper
• Gal4/UAS Transgenic Tools and Their Application to Zebrafish

  2008cites this paper
• Activation Domain-dependent Monoubiquitylation of Gal4 Protein Is Essential for Promoter Binding in Vivo*

  2008cites this paper
• The GAL4 system : a versatile system for the expression of genes.

  2008cites this paper
• Detection and localisation of protein-protein interactions in Saccharomyces cerevisiae using a split-GFP method.

  2008cites this paper
• Galactose metabolism in yeast-structure and regulation of the leloir pathway enzymes and the genes encoding them.

  2008cites this paper
• TFIIB and the regulation of transcription by RNA polymerase II

  2007cites this paper
• Gal11p dosage-compensates transcriptional activator deletions via Taf14p.

  2007cites this paper
• A functional analysis of KlSRB10: implications in Kluyveromyces lactis transcriptional regulation

  2007cites this paper
• Design and isolation of temperature-sensitive mutants of Gal4 in yeast and Drosophila.

  2007cites this paper
• Design and Utility of Temperature-Sensitive Gal4 Mutants for Conditional Gene Expression in Drosophila

  2007cites this paper
• Nine-amino-acid transactivation domain: establishment and prediction utilities.

  2007influential citation
• Galactose Metabolism in Saccharomyces cerevisiae

  2007cites this paper
• The Saccharomyces cerevisiae Phosphatase Activator RRD1 Is Required to Modulate Gene Expression in Response to Rapamycin Exposure

  2006cites this paper
• Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: current status

  2006cites this paper
• Identifizierung von cis-wirkenden Sequenzen in den alternativen HIV-1 Leaderexons und ihre funktionelle Bedeutung für die Spleißregulation

  2006cites this paper
• The Galactose Switch in Kluyveromyces lactis Depends on Nuclear Competition between Gal4 and Gal1 for Gal80 Binding*

  2006cites this paper
• Converting inactive peptides into potent transcriptional activators.

  2006influential citation
• Yeast Gal4: a transcriptional paradigm revisited

  2006cites this paper
• Contribution of Amino Acid Side Chains to Sugar Binding Specificity in a Galactokinase, Gal1p, and a Transcriptional Inducer, Gal3p*

  2006cites this paper
• review Yeast Gal4: a transcriptional paradigm revisited

  2006cites this paper
• The BRCA1 COOH-terminal Region Acts as an RNA Polymerase II Carboxyl-terminal Domain Kinase Inhibitor That Modulates p21WAF1/CIP1 Expression*

  2006cites this paper
• Fluorescence resonance energy transfer as a method for dissecting in vivo mechanisms of transcriptional activation.

  2006cites this paper
• Molecular mechanism of Arabidopsis CBF mediated plant cold-regulated gene transcriptional activation

  2006cites this paper
• Analysis of in vivo targets of transcriptional activators by fluorescence resonance energy transfer.

  2006cites this paper
• Determinants of specificity of MDM2 for the activation domains of p53 and p65: proline27 disrupts the MDM2-binding motif of p53.

  2006cites this paper
• Mitochondrial dysfunction enhances Gal4-dependent transcription.

  2005influential citation
• A comparative analysis of the GAL genetic switch between not-so-distant cousins: Saccharomyces cerevisiae versus Kluyveromyces lactis.

  2005cites this paper
• Binding mode and transcriptional activation potential of high affinity ligands for the CBP KIX domain.

  2005cites this paper
• Targets of the Gal4 Transcription Activator in Functional Transcription Complexes

  2005influential citation
• Functional specificity of artificial transcriptional activators.

  2005cites this paper
• The F box protein Dsg1/Mdm30 is a transcriptional coactivator that stimulates Gal4 turnover and cotranscriptional mRNA processing.

  2005cites this paper
• Transcriptional activating regions target attached substrates to a cyclin-dependent kinase.

  2005cites this paper
• Gal80 Dimerization and the Yeast GAL Gene Switch

  2005cites this paper
• The Saccharomyces cerevisiae Srb8-Srb11 Complex Functions with the SAGA Complex during Gal4-Activated Transcription

  2005cites this paper
• Artificial Transcriptional Activation Domains

  2005cites this paper
• Parallel, quantitative measurement of protein binding to a 120-element double-stranded DNA array in real time using surface plasmon resonance microscopy.

  2004cites this paper
• A small molecule transcriptional activation domain.

  2004cites this paper