Refinement of the Automated Method for Human Islet Isolation and Presentation of a Closed System for In Vitro Islet Culture

No abstract is available for this paper.

• Publication year

  2004

• Venue

  Transplantation

• Publication date

  2004-11-15

• Fields of study

  Medicine

• Identifiers
  DOI 10.1097/01.TP.0000140882.53773.DCPMID 15548977
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Development of a human pancreatic islet-transplant program through a collaborative relationship with a remote islet-isolation center

  2004cited by this paper
• Transplantation of Cultured Islets from Two‐Layer Preserved Pancreases in Type 1 Diabetes with Anti‐CD3 Antibody

  2004cited by this paper
• The optimization of large-scale density gradient isolation of human islets

  2004influential reference
• Successful human islet isolation utilizing recombinant collagenase.

  2003cited by this paper
• Effect of the two-layer (University of Wisconsin solution–perfluorochemical plus O2) method of pancreas preservation on human islet isolation, as assessed by the Edmonton Isolation Protocol1

  2002cited by this paper
• Factors affecting human islet of Langerhans isolation yields.

  2002cited by this paper
• Achievement of insulin independence in three consecutive type-1 diabetic patients via pancreatic islet transplantation using islets isolated at a remote islet isolation center

  2002influential reference
• SERINE-PROTEASE INHIBITION DURING ISLET ISOLATION INCREASES ISLET YIELD FROM HUMAN PANCREASES WITH PROLONGED ISCHEMIA1

  2001cited by this paper
• Islet Transplantation in Seven Patients with Type 1 Diabetes Mellitus Using a Glucocorticoid-Free Immunosuppressive Regimen

  2000cited by this paper
• Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen.

  2000cited by this paper
• Intraductal collagenase delivery into the human pancreas using syringe loading or controlled perfusion.

  1998influential reference
• Improved Human Islet Isolation Using a New Enzyme Blend, Liberase

  1997cited by this paper
• Variables in organ donors that affect the recovery of human islets of Langerhans.

  1996cited by this paper
• Human islet isolation in 104 consecutive cases. Factors affecting isolation success.

  1994cited by this paper
• THE CORRELATION BETWEEN DONOR CHARACTERISTICS AND THE SUCCESS OF HUMAN ISLET ISOLATION1,2

  1994cited by this paper
• Studies of the isolation and viability of human islets of Langerhans.

  1988cited by this paper
• A new, simple method for cold storage of the pancreas using perfluorochemical.

  1988cited by this paper
• Automated Method for Isolation of Human Pancreatic Islets

  1988cited by this paper
• REPRODUCIBLE HIGH YIELD OF RAT ISLETS BY STATIONARY IN VITRO DIGESTION FOLLOWING PANCREATIC DUCTAL OR PORTAL VENOUS COLLAGENASE INJECTION1

  1987cited by this paper
• A Method for Isolation of Islets of Langerhans from the Human Pancreas

  1984cited by this paper
• Preparation of Viable islet Cells from Dogs by a New Method

  1981cited by this paper
• [ON THE QUANTITATIVE DETERMINATION OF COLLAGENASE].

  1963cited by this paper

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• Single-cell proteomics of pancreatic islet cells reveals type 1 diabetes and donor-specific features

  2025cites this paper
• Transcriptomic characterization of human pancreatic CD206- and CD206 + macrophages

  2025cites this paper
• Dynamic recruitment of Munc13 primes docked secretory granules for exocytosis.

  2025cites this paper
• Pancreatic δ-cells are resistant to auto- and paracrine inhibition in human type-2 diabetes

  2025cites this paper
• VAMP8 Is an Endosomal v-SNARE That Supports GLP-1 Receptor Recycling in Pancreatic β-Cells

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• Advancing Diabetes Research: A Novel Islet Isolation Method from Living Donors

  2024cites this paper
• Quantification of Secretory Granule Exocytosis by TIRF Imaging and Capacitance Measurements.

  2023cites this paper
• Local PI(4,5)P2 signaling inhibits fusion pore expansion during exocytosis.

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• Preclinical evaluation of Affibody molecule for PET imaging of human pancreatic islets derived from stem cells

  2023cites this paper
• [18F]MK-7246 for Positron Emission Tomography Imaging of the Beta-Cell Surface Marker GPR44

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• Mesenchymal Stem Cells Secretions Enhanced ATP Generation on Isolated Islets during Transplantation

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• Altered microvasculature in pancreatic islets from subjects with type 1 diabetes

  2022cites this paper
• Optimization of dye solutions for detecting damaged pancreatic tissues during islet isolation procedures

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• Hypothermic oxygenated machine perfusion of the human pancreas for clinical islet isolation: a prospective feasibility study

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• Autologous regulatory T cells in clinical intraportal allogenic pancreatic islet transplantation

  2021cites this paper
• Feasibility of large experimental animal models in testing novel therapeutic strategies for diabetes

  2021cites this paper
• Improvement of hepatocyte engraftment by co‐transplantation with pancreatic islets in hepatocyte transplantation

  2021cites this paper
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  2021cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
• Strategies to improve islet yield from chronic pancreatitis pancreases intended for islet autotransplantation

  2020cites this paper
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  2020cites this paper
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  2020cites this paper
• Heterogeneity of Human Pancreatic Islet Isolation Around Europe: Results of a Survey Study

  2020cites this paper
• A whole organism small molecule screen identifies novel regulators of pancreatic endocrine development

  2019cites this paper
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  2019cites this paper
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  2019cites this paper
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  2019cites this paper
• Strategy towards tailored donor tissue-specific pancreatic islet isolation

  2019cites this paper
• Islet isolation for autotransplantation, following total or near total pancreatectomy

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• Open Randomized Multicenter Study to Evaluate Safety and Efficacy of Low Molecular Weight Sulfated Dextran in Islet Transplantation

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  2019cites this paper
• The PI(4)P phosphatase Sac2 controls insulin granule docking and release

  2019cites this paper
• Recruited fibroblasts reconstitute the peri-islet membrane: a longitudinal imaging study of human islet grafting and revascularisation

  2019cites this paper
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  2019cites this paper
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  2018cites this paper
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  2018cites this paper
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  2018cites this paper
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  2018cites this paper
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  2018cites this paper
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  2018cites this paper
• Expression profiles of stress-related genes in islets from donors with progressively impaired glucose metabolism

  2018cites this paper
• Comparison of Clostripain and Neutral Protease as Supplementary Enzymes for Human Islet Isolation

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  2018cites this paper
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• In Vivo Visualization of β-Cells by Targeting of GPR44

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• GPR44 is a pancreatic protein restricted to the human beta cell

  2016cites this paper
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  2016cites this paper
• Cellular Immunological Mechanisms Contributing to Diabetes Pathogenesis

  2016cites this paper
• Sericin in the isolating solution improves the yield of islets isolated from the pancreas

  2016cites this paper
• The pathogenesis of type 1 diabetes - lessons from pancreatic biopsies in the Diabetes Virus Detection Study (DiViD)

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• Insulitis in human diabetes: a histological evaluation of donor pancreases

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• Altered serotonin (5-HT) 1D and 2A receptor expression may contribute to defective insulin and glucagon secretion in human type 2 diabetes.

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• Islet Culture/Preservation Before Islet Transplantation.

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• Assessment of pancreatic islet cell function and survival

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