Identification and characterization of the CONSTANS-like gene family and its expression profiling under light treatment in Populus.

The CONSTANS-like (COL) genes play an important role in the photoperiodic flowering pathway. Poplar is a perennial woody plant with a long juvenile phase, but the molecular characterization of COL genes in Populus is limited. In this study, 14 COL genes were identified in the Populus genome. Phylogenetic analysis indicated the PtCOL proteins were divided into three subgroups, and the members of each subgroup had similar gene structure and motif composition. Chromosome distribution analysis showed that 14 PtCOL genes were distributed on 10 chromosomes. Multiple sequence alignment indicated that these proteins contained a highly conserved B-box1 and a conserved CCT domain, but the B-box2 structure was divided into three different types. Promoter analysis found that there were several light-responsive cis-elements in the PtCOL genes. Furthermore, tissue-specific expression showed that all nine PtCOL genes were widely expressed in various tissues and organs of Populus, and were preferentially expressed in the leaves. Additionally, the transcription level of PtCOL exhibited a diurnal oscillation pattern in different light conditions. This study not only provided comprehensive information for further analysis of the function of the PtCOL gene family, but also revealed the biological roles of PtCOL genes in the photoperiod-dependent flowering process of Populus.

• Publication year

  2020

• Venue

  International Journal of Biological Macromolecules

• Publication date

  2020-06-09

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1016/j.ijbiomac.2020.06.056PMID 32531358
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The CONSTANS flowering complex controls the protective response of photosynthesis in the green alga Chlamydomonas

  2019cited by this paper
• The Arabidopsis thaliana CONSTANS-LIKE 4 (COL4) – A Modulator of Flowering Time

  2019cited by this paper
• Molecular Cloning and Functional Analysis of Three CONSTANS-Like Genes from Chinese Cymbidium

  2019cited by this paper
• Genome-wide identification of the HKT genes in five Rosaceae species and expression analysis of HKT genes in response to salt-stress in Fragaria vesca

  2018cited by this paper
• Genome-wide Identification, Phylogenetic Analysis, and Expression Profiling of CONSTANS-like (COL) Genes in Vitis vinifera

  2018cited by this paper
• Molecular characterization of a CONSTANS gene from Sapium sebiferum (L.) Rxob.

  2018cited by this paper
• The rice CONSTANS-like protein OsCOL15 suppresses flowering by promoting Ghd7 and repressing RID1.

  2018cited by this paper
• Isolation and functional characterization of a circadian-regulated CONSTANS homolog (GbCO) from Ginkgo biloba

  2017cited by this paper
• OsCOL16, encoding a CONSTANS-like protein, represses flowering by up-regulating Ghd7 expression in rice.

  2017cited by this paper
• Identification and characterization of CONSTANS-like (COL) gene family in upland cotton (Gossypium hirsutum L.)

  2017cited by this paper
• OsCOL10, a CONSTANS-Like Gene, Functions as a Flowering Time Repressor Downstream of Ghd7 in Rice.

  2016cited by this paper
• A CONSTANS-like transcriptional activator, OsCOL13, functions as a negative regulator of flowering downstream of OsphyB and upstream of Ehd1 in rice

  2016cited by this paper
• MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets.

  2016cited by this paper
• Molecular characterization of CONSTANS-Like (COL) genes in banana (Musa acuminata L. AAA Group, cv. Grand Nain)

  2016cited by this paper
• Identification and characterization of the CONSTANS-like gene family in the short-day plant Chrysanthemum lavandulifolium

  2015cited by this paper
• The Phyre2 web portal for protein modeling, prediction and analysis

  2015cited by this paper
• The CONSTANS-like 4 transcription factor, AtCOL4, positively regulates abiotic stress tolerance through an abscisic acid-dependent manner in Arabidopsis.

  2015cited by this paper
• CONSTANS is a photoperiod regulated activator of flowering in sorghum

  2014cited by this paper
• GSDS 2.0: an upgraded gene feature visualization server

  2014cited by this paper
• Functional and Evolutionary Characterization of the CONSTANS Gene Family in Short-Day Photoperiodic Flowering in Soybean

  2014cited by this paper
• Isolation and functional analysis of CONSTANS-LIKE genes suggests that a central role for CONSTANS in flowering time control is not evolutionarily conserved in Medicago truncatula

  2014cited by this paper
• CONSTANS-LIKE 7 (COL7) is involved in phytochrome B (phyB)-mediated light-quality regulation of auxin homeostasis.

  2014cited by this paper
• The BBX family of plant transcription factors.

  2014cited by this paper
• Clock-Controlled and FLOWERING LOCUS T (FT)-Dependent Photoperiodic Pathway in Lotus japonicus I: Verification of the Flowering-Associated Function of an FT Homolog

  2013cited by this paper
• CONSTANS-LIKE 7 regulates branching and shade avoidance response in Arabidopsis

  2013cited by this paper
• The differential expression of HvCO9, a member of the CONSTANS-like gene family, contributes to the control of flowering under short-day conditions in barley

  2011cited by this paper
• Characterization and transgenic study of CONSTANS-LIKE8 (COL8) gene in Arabidopsis thaliana: expression of 35S:COL8 delays flowering under long-day conditions

  2011cited by this paper
• Isolation of a LEAFY homolog from Populus tomentosa: expression of PtLFY in P. tomentosa floral buds and PtLFY-IR-mediated gene silencing in tobacco (Nicotiana tabacum)

  2010cited by this paper
• OsCOL4 is a constitutive flowering repressor upstream of Ehd1 and downstream of OsphyB.

  2010cited by this paper
• Over-expression of CONSTANS-LIKE 5 can induce flowering in short-day grown Arabidopsis

  2009cited by this paper
• Functional analysis of 5′ untranslated region of a TIR-NBS-encoding gene from triploid white poplar

  2009cited by this paper
• Identification of PaCOL1 and PaCOL2, two CONSTANS-like genes showing decreased transcript levels preceding short day induced growth cessation in Norway spruce.

  2009cited by this paper
• Sugar beet contains a large CONSTANS-LIKE gene family including a CO homologue that is independent of the early-bolting (B) gene locus

  2008cited by this paper
• Regulation and identity of florigen: FLOWERING LOCUS T moves center stage.

  2008cited by this paper
• OsCO3, a CONSTANS-LIKE gene, controls flowering by negatively regulating the expression of FT-like genes under SD conditions in rice

  2008cited by this paper
• SMART 6: recent updates and new developments

  2008cited by this paper
• Characterization of resistance gene analogs with a nucleotide binding site isolated from a triploid white poplar.

  2008cited by this paper
• Clustal W and Clustal X version 2.0

  2007cited by this paper
• Move on up, it's time for change--mobile signals controlling photoperiod-dependent flowering.

  2007cited by this paper
• MEME: discovering and analyzing DNA and protein sequence motifs

  2006cited by this paper
• The Genome of Black Cottonwood, Populus trichocarpa (Torr. & Gray)

  2006cited by this paper
• CONSTANS and the CCAAT Box Binding Complex Share a Functionally Important Domain and Interact to Regulate Flowering of Arabidopsis[W][OA]

  2006cited by this paper
• CO/FT Regulatory Module Controls Timing of Flowering and Seasonal Growth Cessation in Trees

  2006cited by this paper
• Genome-Wide Analysis of the ERF Gene Family in Arabidopsis and Rice[W]

  2006cited by this paper
• FKF1 F-Box Protein Mediates Cyclic Degradation of a Repressor of CONSTANS in Arabidopsis

  2005cited by this paper
• The family of CONSTANS-like genes in Physcomitrella patens.

  2005cited by this paper
• Arabidopsis CONSTANS-LIKE3 Is a Positive Regulator of Red Light Signaling and Root Growth[W]

  2005cited by this paper
• Oscillation of mRNA level and activity of granule-bound starch synthase I in Arabidopsis leaves during the day/night cycle

  2003cited by this paper
• The Evolution of CONSTANS-Like Gene Families in Barley, Rice, and Arabidopsis1

  2003cited by this paper
• PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences

  2002cited by this paper
• Functional importance of conserved domains in the flowering-time gene CONSTANS demonstrated by analysis of mutant alleles and transgenic plants.

  2002cited by this paper
• Analysis of the function of two circadian-regulated CONSTANS-LIKE genes.

  2001cited by this paper
• CONSTANS mediates between the circadian clock and the control of flowering in Arabidopsis

  2001cited by this paper
• Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

  2001cited by this paper
• Rapid evolution of the family of CONSTANS LIKE genes in plants.

  2000cited by this paper
• RING fingers and B-boxes: zinc-binding protein-protein interaction domains.

  1998cited by this paper
• The CONSTANS gene of Arabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factors.

  1995cited by this paper

• Transcription factor Constans-like 4 may regulate tomato fruit ripening by interacting with abscisic acid and ethylene signaling

  2025cites this paper
• Comprehensive Analysis of the R2R3-MYB Transcription Factor Gene Family in Salvia miltiorrhiza and the Regulatory Role of SmMYB50 in Salvianolic Acid Metabolism

  2025cites this paper
• A transcriptional roadmap of the yearly growth cycle in Populus trees

  2025cites this paper
• Genome-wide identification of CONSTANS-like genes in Dimocarpus longan and functional characterization of DlCOL9 revealing its role in floral induction.

  2025cites this paper
• CONSTANS-like 13 homologs MiCOL13 A and MiCOL13B orchestrate flowering time and salt-drought tolerance in mango

  2025cites this paper
• Genome-wide identification and functional analysis of the longan CONSTANS (CO) family

  2025cites this paper
• MiCOL6, MiCOL7A and MiCOL7B isolated from mango regulate flowering and stress response in transgenic Arabidopsis.

  2024cites this paper
• Four ClEF1A genes involved in self-incompatibility in 'Xiangshui Lemon' confer early fowering and increase stress tolerance in transgenic Arabidopsis.

  2024cites this paper
• Genome-wide analysis of the CCT gene family and functional characterization of SlCCT6 in response to drought stress in tomato.

  2024cites this paper
• Regulatory frameworks involved in the floral induction, formation and developmental programming of woody horticultural plants: a case study on blueberries

  2024cites this paper
• Genome-wide identification of the Pinus tabuliformis CONSTANS-like gene family and their potential roles in reproductive cone development.

  2023cites this paper
• Genome-wide identification of the MATE gene family and functional characterization of PbrMATE9 related to anthocyanin in pear

  2023cites this paper
• Genome-Wide Identification, Expression, and Molecular Characterization of the CONSTANS-like Gene Family in Seven Orchid Species

  2023cites this paper
• Genome-wide identification, characterization, and expression analysis of CCT transcription factors in poplar.

  2023cites this paper
• Expansion of CONSTANS-like genes in sunflower confers putative neofunctionalization in the adaptation to abiotic stresses

  2022cites this paper
• Overexpression of two CONSTANS-like 2 (MiCOL2) genes from mango delays flowering and enhances tolerance to abiotic stress in transgenic Arabidopsis.

  2022cites this paper
• Transcriptome-wide profiling of mRNA N6-methyladenosine modification in rice panicles and flag leaves.

  2022cites this paper
• Genome-wide identification of the mango CONSTANS (CO) family and functional analysis of two MiCOL9 genes in transgenic Arabidopsis

  2022cites this paper
• Characterization of Phytohormones and Transcriptomic Profiling of the Female and Male Inflorescence Development in Manchurian Walnut (Juglans mandshurica Maxim.)

  2022cites this paper
• Comprehensive Analyses of Four PtoNF-YC Genes from Populus tomentosa and Impacts on Flowering Timing

  2022cites this paper
• Isolation and functional analysis of two CONSTANS-like 1 genes from mango.

  2022cites this paper
• Genome-wide identification, expression, and sequence analysis of CONSTANS-like gene family in cannabis reveals a potential role in plant flowering time regulation

  2021influential citation
• Reproductive developmental transcriptome analysis of Tripidium ravennae (Poaceae)

  2021influential citation
• Conservation and Divergence of the CONSTANS-Like (COL) Genes Related to Flowering and Circadian Rhythm in Brassica napus

  2021cites this paper
• Genome-wide analysis of the CCT gene family in Chinese white pear (Pyrus bretschneideri Rehd.) and characterization of PbPRR2 in response to varying light signals

  2021cites this paper
• Genome-Wide Identification and Functional Characterization of the Trans-Isopentenyl Diphosphate Synthases Gene Family in Cinnamomum camphora

  2021cites this paper