A Potently Neutralizing Antibody Protects Mice against SARS-CoV-2 Infection

Key Points Generation and screening of anti–SARS-CoV-2 RBD mAbs reveal a potently protective Ab. mAb 2B04 potently neutralizes SARS-CoV-2 in vitro. mAb 2B04 protects mice from morbidity in a murine model of SARS-CoV-2 infection. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for millions of infections and hundreds of thousands of deaths globally. There are no widely available licensed therapeutics against SARS-CoV-2, highlighting an urgent need for effective interventions. The virus enters host cells through binding of a receptor-binding domain within its trimeric spike glycoprotein to human angiotensin-converting enzyme 2. In this article, we describe the generation and characterization of a panel of murine mAbs directed against the receptor-binding domain. One mAb, 2B04, neutralized wild-type SARS-CoV-2 in vitro with remarkable potency (half-maximal inhibitory concentration of <2 ng/ml). In a murine model of SARS-CoV-2 infection, 2B04 protected challenged animals from weight loss, reduced lung viral load, and blocked systemic dissemination. Thus, 2B04 is a promising candidate for an effective antiviral that can be used to prevent SARS-CoV-2 infection.

• Publication year

  2020

• Venue

  Journal of Immunology

• Publication date

  2020-06-26

• Fields of study

  Medicine

• Identifiers
  DOI 10.4049/jimmunol.2000583PMID 32591393PMCID PMC7566074
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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