In vivo regulation of rat neutrophil apoptosis occurring spontaneously or induced with TNF-alpha or cycloheximide.

We previously demonstrated that human TNF-alpha induces rapid apoptosis of human neutrophils. To understand better the in vivo significance of neutrophil apoptosis, we examined spontaneous, recombinant human and mouse TNF-alpha- or cycloheximide-induced apoptosis of normal peripheral blood neutrophils (PBN), PBN from rats injected i.p. with proteose peptone or a streptococcus preparation, OK-432 (inflammatory PBN), peritoneally exudated neutrophils (PEN) obtained after a proteose peptone injection, and normal bone marrow neutrophils. The following observations were made. 1) Normal PBN responded to TNF-alpha, but PEN, normal bone marrow neutrophils, and inflammatory PBN at 12 h after stimulation did not. 2) The sensitivity to TNF-alpha of the inflammatory PBN started to decrease at 3 h, was lowest at 12 h, and was almost restored at 52 h after stimulation. 3) Spontaneous apoptosis of normal and inflammatory PBN reached 25% at 12 h after in vitro incubation, but that of PEN and normal bone marrow neutrophils was very low over this period. 4) The sensitivity to cycloheximide (6 h incubation) was high for normal PBN and bone marrow neutrophils, but low for PEN and inflammatory PBN after 12 h. 5) 125I-rhTNF-alpha binding of bone marrow neutrophils was significantly lower than that of normal and inflammatory PBN and PEN. 6) TNF-alpha-induced apoptosis of normal or inflammatory PBN and bone marrow neutrophils was enhanced by treatment with low doses of cycloheximide that alone were barely able to induce neutrophil apoptosis; however, apoptosis of PEN was not. The mechanisms and in vivo significance of these phenomena are discussed.

• Publication year

  1995

• Venue

  Journal of Immunology

• Publication date

  1995-03-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.4049/jimmunol.154.5.2403PMID 7868906
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Modulation of in vivo immune response by selective depletion of neutrophils using a monoclonal antibody, RP-3. I. Inhibition by RP-3 treatment of the priming and effector phases of delayed type hypersensitivity to sheep red blood cells in rats.

  1993cited by this paper
• Rapid acceleration of neutrophil apoptosis by tumor necrosis factor-alpha.

  1993cited by this paper
• Interleukin-1 type II receptor: a decoy target for IL-1 that is regulated by IL-4.

  1993cited by this paper
• Prolongation of survival of human polymorphonuclear neutrophils by granulocyte-macrophage colony-stimulating factor is caused by inhibition of programmed cell death.

  1992cited by this paper
• Modulation of granulocyte survival and programmed cell death by cytokines and bacterial products.

  1992cited by this paper
• Inhibition of IL-8-induced W3/25+ (CD4+) T lymphocyte recruitment into subcutaneous tissues of rats by selective depletion of in vivo neutrophils with a monoclonal antibody.

  1991cited by this paper
• Induction of the heat shock response protects cells from lysis by tumor necrosis factor.

  1990cited by this paper
• Impaired superoxide production in peripheral blood neutrophils of germ-free rats.

  1990cited by this paper
• Shedding of tumor necrosis factor receptors by activated human neutrophils

  1990cited by this paper
• Binding and regulation of cellular functions by monoclonal antibodies against human tumor necrosis factor receptors

  1990cited by this paper
• Modulation of the immune response to transplanted tumors in rats by selective depletion of neutrophils in vivo using a monoclonal antibody: abrogation of specific transplantation resistance to chemical carcinogen-induced syngeneic tumors by selective depletion of neutrophils in vivo.

  1990cited by this paper
• Manganous superoxide dismutase is essential for cellular resistance to cytotoxicity of tumor necrosis factor.

  1989cited by this paper
• Production of interleukin-1-alpha and -beta by human peripheral polymorphonuclear neutrophils.

  1989cited by this paper
• Isolation and Characterization of Interleukin‐1 From Bovine Polymorphonuclear Leukocytes

  1989cited by this paper
• Granulocyte-macrophage colony-stimulating factor induces cytokine secretion by human polymorphonuclear leukocytes.

  1989cited by this paper
• Purification and partial sequence of rabbit polymorphonuclear leukocyte-derived lymphocyte proliferation potentiating factor resembling IL-1 beta.

  1988cited by this paper
• Induction of manganous superoxide dismutase by tumor necrosis factor: possible protective mechanism

  1988cited by this paper
• Inhibitors of protein synthesis and RNA synthesis prevent neuronal death caused by nerve growth factor deprivation

  1988cited by this paper
• Activation of human polymorphonuclear neutrophil functions by interferon-gamma and tumor necrosis factors.

  1985cited by this paper
• Neutrophil elastase-releasing factors in bronchoalveolar lavage from patients with adult respiratory distress syndrome.

  1985cited by this paper
• Activation of cytotoxic polymorphonuclear leukocytes by in vivo administration of a streptococcal preparation, OK-432.

  1984cited by this paper
• Development of the adult respiratory distress syndrome: progressive alteration of neutrophil chemotactic and secretory processes.

  1984cited by this paper
• Kinetics of neutrophil accumulation in acute inflammatory lesions induced by chemotaxins and chemotaxinigens.

  1984cited by this paper
• Desensitization of acute inflammatory lesions to chemotaxins and endotoxin.

  1984cited by this paper
• In vitro induction of cytotoxic polymorphonuclear leukocytes by supernatant from a concanavalin A-stimulated spleen cell culture.

  1983cited by this paper
• Studies on the anticancer and streptolysin S-forming abilities of hemolytic streptococci.

  1967cited by this paper

• Intracellular Major Histocompatibility Complex Class II and C-X-C Motif Chemokine Ligand 10-Expressing Neutrophils Indicate the State of Anti-Tumor Activity Induced by Bacillus Calmette–Guérin

  2023cites this paper
• Amaranthus spinosus L. inhibits spontaneous and dexamethasone-induced apoptosis in murine primary splenocytes

  2020cites this paper
• The TLR4‐PAR1 Axis Regulates Bone Marrow Mesenchymal Stromal Cell Survival and Therapeutic Capacity in Experimental Bacterial Pneumonia

  2018cites this paper
• The TLR4-PAR1 Axis Regulates Bone Marrow Mesenchymal Stromal Cell Survival and Therapeutic Capacity in Murine E. coli Pneumonia

  2018cites this paper
• Stabilization of Hypoxia-Inducible Factor-1 Alpha Augments the Therapeutic Capacity of Bone Marrow-Derived Mesenchymal Stem Cells in Experimental Pneumonia

  2018cites this paper
• Systemic neutrophil migration and rapid consumption of neutrophils in the spleen

  2018cites this paper
• The TLR4-PAR1 Axis Regulates Bone Marrow Mesenchymal Stromal Cell Survival and Therapeutic Capacity in Murine E. coli Pneumonia

  2018cites this paper
• Tissue influx of neutrophils and monocytes is delayed during development of trovafloxacin‐induced tumor necrosis factor‐dependent liver injury in mice

  2018cites this paper
• Cell-based in vitro models in environmental toxicology: a review

  2017cites this paper
• High Throughput Analysis of Golgi Structure by Imaging Flow Cytometry

  2017cites this paper
• The Tumor Suppressor Hace1 Is a Critical Regulator of TNFR1-Mediated Cell Fate

  2016cites this paper
• The Tumor Suppressor Hace1 Is a Critical Regulator of TNFR1-Mediated Cell Fate

  2016cites this paper
• Intervention of pentoxifylline on silica-induced inflammatory reaction and apoptosis

  2015cites this paper
• Bacteria Induce Prolonged PMN Survival via a Phosphatidylcholine-Specific Phospholipase C- and Protein Kinase C-Dependent Mechanism

  2014cites this paper
• PHYTOCHEMICAL STUDIES AND ANTI-BACTERIAL ACTIVITIES OF AMARANTHUS SPINOSUS.L

  2014cites this paper
• Ethyl pyruvate ameliorates endotoxin-induced corneal inflammation.

  2012cites this paper
• Virus-infected alveolar epithelial cells direct neutrophil chemotaxis and inhibit their apoptosis.

  2012cites this paper
• Translation inhibitors and their unique biological properties.

  2012cites this paper
• Peculiarities of cell death mechanisms in neutrophils

  2011cites this paper
• Macrophage phagocytosis of neutrophils at inflammatory/infectious foci: a cooperative mechanism in the control of infection and infectious inflammation

  2011cites this paper
• Caspase-8 Mediates Mitochondrial Release of Pro-apoptotic Proteins in a Manner Independent of Its Proteolytic Activity in Apoptosis Induced by the Protein Synthesis Inhibitor Acetoxycycloheximide in Human Leukemia Jurkat Cells*

  2009cites this paper
• Role of Bcl-2 proteins in neutrophil activation and delayed apoptosis in crystal-induced arthritis

  2008cites this paper
• Identification and characterization of infiltrating macrophages in acetaminophen‐induced liver injury

  2008cites this paper
• A study of innate antiviral mechanisms using fish cell lines

  2006cites this paper
• Macrophages Induce Neutrophil Apoptosis through Membrane TNF, a Process Amplified by Leishmania major1

  2006cites this paper
• Cyclic GMP-dependent protein kinase and soluble guanylyl cyclase disappear in elicited rat neutrophils.

  2006cites this paper
• Preferential induction of apoptosis in the rainbow trout macrophage cell line, RTS11, by actinomycin D, cycloheximide and double stranded RNA.

  2005cites this paper
• High expression of Bcl-xL in delayed apoptosis of canine neutrophils induced by lipopolysaccharide.

  2005cites this paper
• Acetoxycycloheximide (E-73) rapidly induces apoptosis mediated by the release of cytochrome c via activation of c-Jun N-terminal kinase.

  2005cites this paper
• Activation of extracellular signal-regulated kinase couples platelet-activating factor-induced adhesion and delayed apoptosis of human neutrophils.

  2004cites this paper
• Decreased apoptotic polymorphonuclear leukocyte rate in dogs with pyometra.

  2004cites this paper
• Intraperitoneal Injection of Lipopolysaccharide Induces Dynamic Migration of Gr-1high Polymorphonuclear Neutrophils in the Murine Abdominal Cavity

  2004cites this paper
• Human MUC1 carcinoma-associated protein confers resistance to genotoxic anticancer agents.

  2004cites this paper
• Stably transfected human cell lines as fluorescent screening assay for nuclear factor KB activation dependent gene expression

  2004cites this paper
• Regulation of systemic and local neutrophil responses by G-CSF during pulmonary Pseudomonas aeruginosa infection.

  2004cites this paper
• Down-Regulation of the Immune Response

  2003cites this paper
• Molecular Basis for Microcirculatory Disorders

  2003cites this paper
• Generation of Stably Transfected Mammalian Cell Lines as Fluorescent Screening Assay for NF- B Activation-Dependent Gene Expression

  2003cites this paper
• Neutrophil Restraint by Green Tea: Inhibition of Inflammation, Associated Angiogenesis, and Pulmonary Fibrosis1

  2003cites this paper
• Inhibition of Inflammation, Associated Neutrophil Restraint by Green Tea:

  2003cites this paper
• Mechanisms of immune resolution.

  2003cites this paper
• Regulation of Neutrophil Apoptosis

  2003cites this paper
• Regulation of neutrophil apoptosis.

  2003cites this paper
• This information is current as Angiogenesis , and Pulmonary Fibrosis Inhibition of Inflammation , Associated Neutrophil Restraint by Green Tea : Spiridione Garbisa

  2003cites this paper
• Elucidation of Molecular Events Leading to Neutrophil Apoptosis following Phagocytosis

  2003cites this paper
• PATHOLOGICAL ASPECTS OF THE ANTI-INFLAMMATORY/IMMUNE SUPPRESSIVE RESPONSE IN SEPSIS AND SHOCK.

  2003cites this paper
• Fas-Ligand Mediated Apoptosis in Severe Sepsis and Shock

  2003cites this paper
• Role of reactive oxygen species in neutrophil apoptosis following ingestion of heat‐killed Staphylococcus aureus

  2002cites this paper
• A Novel Therapeutic Strategy for Attenuating Neutrophil-Mediated Lung Injury In Vivo

  2002cites this paper
• Predicting drug-induced agranulocytosis: characterizing neutrophil-generated metabolites of a model compound, DMP 406, and assessing the relevance of an in vitro apoptosis assay for identifying drugs that may cause agranulocytosis.

  2002cites this paper
• Restraint Stress-Induced Elevation of Endogenous Glucocorticoids Decreases Peyer’s Patch Cell Numbers via Mechanisms That Are either Dependent or Independent on Apoptotic Cell Death

  2002cites this paper
• ResponseResults in a Diminished Acute Inflammatory Deficiency in the Anti-Apoptotic Protein A1-a

  2002cites this paper
• Deficiency in the Anti-Apoptotic Protein A1-a Results in a Diminished Acute Inflammatory Response1

  2002cites this paper
• Apoptosis of bovine neutrophils during mastitis experimentally induced with Escherichia coli or endotoxin.

  2002cites this paper
• BCL‐2 family expression in human neutrophils during delayed and accelerated apoptosis

  2001cites this paper
• Divergent effects of tumor necrosis factor alpha on apoptosis of human neutrophils.

  2001cites this paper
• Effects of phospholipid surfactant on apoptosis induction by respirable quartz and kaolin in NR8383 rat pulmonary macrophages.

  2001cites this paper
• Understanding the role of caspase-3 in vivo

  2001cites this paper
• Neutrophils induce apoptosis of lung epithelial cells via release of soluble Fas ligand.

  2001cites this paper
• The loss of susceptibility to apoptosis in exudated tissue neutrophils is associated with their nuclear factor-kappa B activation.

  2001cites this paper
• Divergent effects of tumor necrosis factor α on apoptosis of human neutrophils

  2001cites this paper
• Apoptosis in cells of bronchoalveolar lavage: A cellular reaction in patients who die with sepsis and respiratory failure

  2001cites this paper
• Inhibition of TNF-α-Induced Neutrophil Apoptosis by Crystals of Calcium Pyrophosphate Dihydrate Is Mediated by the Extracellular Signal-Regulated Kinase and Phosphatidylinositol 3-Kinase/Akt Pathways Up-Stream of Caspase 31

  2000cites this paper
• Immunohistochemical Study on Fas and Fas Ligand in Skin Wound Healing

  2000cites this paper
• Macrophage-Induced Neutrophil Apoptosis1

  2000cites this paper
• Pulmonary neutrophil recruitment, activation and clearance in equine chronic obstructive pulmonary disease

  2000cites this paper
• Thalidomide analogue CC‐3052 reduces HIV+ neutrophil apoptosis in vitro

  2000cites this paper
• Macrophage-Induced Neutrophil Apoptosis

  2000cites this paper
• Nuclear factor-kappaB activates dual inhibition sites in the regulation of tumor necrosis factor-alpha-induced neutrophil apoptosis.

  2000cites this paper
• Role of hypothermia induced by tumor necrosis factor on apoptosis and function of inflammatory neutrophils in mice.

  2000influential citation
• Counterregulation of Severe Inflammation: When More is Too Much and Less is Inadequate

  2000cites this paper
• Antineutrophil antibodies associated with ulcerative colitis interact with the antigen(s) during the process of apoptosis

  2000cites this paper
• The inhibition of spontaneous and tumor necrosis factor-alpha induced neutrophil apoptosis by crystals of calcium pyrophosphate dihydrate and monosodium urate monohydrate.

  2000cites this paper
• Nuclear factor-κB activates dual inhibition sites in the regulation of tumor necrosis factor-α-induced neutrophil apoptosis

  2000cites this paper
• Modulation of neutrophil apoptosis by granulocyte colony‐stimulating factor and granulocyte/macrophage colony‐stimulating factor during the course of acute respiratory distress syndrome

  2000cites this paper
• Dizygotic twin sisters with myelokathexis: Mechanism of its neutropenia

  1999cites this paper
• Evaluation of the influence of N-nitrosodimethyloamine (NDMA) on the apoptosis of neutrophils of peripheral blood (PMN) and the expression of the IL-6R membrane receptor--in vitro research.

  1999cites this paper
• Ethanol‐induced neutrophil apoptosis is mediated through nitric oxide

  1999cites this paper
• Calpain and calpastatin regulate neutrophil apoptosis

  1999cites this paper
• CCAAT/enhancer binding protein epsilon is critical for effective neutrophil-mediated response to inflammatory challenge.

  1999cites this paper
• Expression of scavenger receptor class A and CD14 in lipopolysaccharide‐induced lung injury

  1999cites this paper
• Flow cytometric procedure to detect apoptosis of bovine polymorphonuclear leukocytes in blood.

  1999cites this paper
• The murine antiapoptotic protein A1 is induced in inflammatory macrophages and constitutively expressed in neutrophils.

  1999cites this paper
• Cycloheximide-induced T-cell Death Is Mediated by a Fas-associated Death Domain-dependent Mechanism*

  1999cites this paper
• Apoptosis and Inflammation

  1999cites this paper
• Caspases mediate tumor necrosis factor-alpha-induced neutrophil apoptosis and downregulation of reactive oxygen production.

  1999cites this paper
• Inhibition of tumor necrosis factor-alpha induced neutrophil apoptosis by cyclic AMP: involvement of caspase cascade.

  1999cites this paper
• Effect of manoalide on apoptosis induced by deprivation of growth factors in vascular endothelial cells.

  1999cites this paper
• Glucocorticoid hormone suppression of human neutrophil‐mediated tumor cell cytostasis

  1999cites this paper
• Apoptosis: Its role in the systemic inflammatory response syndrome and the involvement of cytokines

  1999cites this paper
• CCAAT/Enhancer Binding Protein ɛ Is Critical for Effective Neutrophil-Mediated Response to Inflammatory Challenge

  1999cites this paper
• Constitutively Expressed in Neutrophils Induced in Inflammatory Macrophages and The Murine Antiapoptotic Protein A1 Is

  1999cites this paper
• Apoptosis is induced in glioma cells by antisense oligonucleotides to protein kinase Cα and is enhanced by cycloheximide

  1998cites this paper
• Chemotaxis, Phagocytosis and Killing of Monocytes under Moderate Hypothermia

  1998cites this paper
• Effects of glucocorticoids on apoptosis of infiltrated eosinophils and neutrophils in rats.

  1998cites this paper
• Priming, second-hit priming, and apoptosis in leukocytes from trauma patients.

  1998cites this paper
• Fas ligand (gld)‐ and Fas (lpr)‐deficient mice do not show alterations in the extravasation or apoptosis of inflammatory neutrophils

  1998cites this paper
• Accelerated Neutrophil Apoptosis in Mice Lacking A1-a, a Subtype of the bcl-2–related A1 Gene

  1998cites this paper
• Resistance to DNA fragmentation and chromatin condensation in mice lacking the DNA fragmentation factor 45.

  1998cites this paper
• Characteristics of the immunological response in the clearance of non‐typeable Haemophilus influenzae from the lung

  1998cites this paper