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Tagging Transferrin Receptor with a Disulfide FRET Probe to Gauge the Redox State in Endosomal Compartments.

Xiaobao Bi,Juan Yin,Dingpeng Zhang,Xiaohong Zhang,S. Balamkundu,J. Lescar,P. Dedon,J. Tam,Chuan-fa Liu

Published 2020 in Analytical Chemistry

ABSTRACT

Although the basic process of receptor-mediated endocytosis (RME) is well established, certain specific aspects, like the endosomal redox state, remain less characterized. Previous studies used chemically labeled ligands or antibodies with a FRET (Fluorescence Resonance Energy Transfer) probe to gauge the redox activity of the endocytic pathway, with a limitation being their inability to track the apo receptor. New tools that allow direct labeling of a cell-surface receptor with synthetic probes would aid in the study of its endocytic pathway and function. Herein, we use a peptide ligase, butelase 1, to label the human transferrin receptor 1 (TfR1) in established human cell lines with a designer disulfide FRET probe. This strategy enables us to obtain real-time live cell imaging of redox states in TfR1-mediated endocytosis, attesting a reducing environment in the endoso-mal compartments and the dynamics of TfR1 trafficking. A better understanding of endocytosis of different cell surface receptors has implications in designing strategies that hijack this natural process for intracellular drug delivery.

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