Advances in Plant Regeneration: Shake, Rattle and Roll

Some plant cells are able to rebuild new organs after tissue damage or in response to definite stress treatments and/or exogenous hormone applications. Whole plants can develop through de novo organogenesis or somatic embryogenesis. Recent findings have enlarged our understanding of the molecular and cellular mechanisms required for tissue reprogramming during plant regeneration. Genetic analyses also suggest the key role of epigenetic regulation during de novo plant organogenesis. A deeper understanding of plant regeneration might help us to enhance tissue culture optimization, with multiple applications in plant micropropagation and green biotechnology. In this review, we will provide additional insights into the physiological and molecular framework of plant regeneration, including both direct and indirect de novo organ formation and somatic embryogenesis, and we will discuss the key role of intrinsic and extrinsic constraints for cell reprogramming during plant regeneration.

• Publication year

  2020

• Venue

  Plants

• Publication date

  2020-07-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.3390/plants9070897PMID 32708602PMCID 7412315
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Reprogramming of Cell Fate During Root Regeneration by Transcriptional and Epigenetic Networks

  2020cited by this paper
• Determinants of PB1 Domain Interactions in Auxin Response Factor ARF5 and Repressor IAA17.

  2020cited by this paper
• The Type-B Cytokinin Response Regulator ARR1 Inhibits Shoot Regeneration in an ARR12-Dependent Manner in Arabidopsis[OPEN]

  2020cited by this paper
• Plant cell totipotency: Insights into cellular reprogramming.

  2020cited by this paper
• The atypical histone variant H3.15 promotes callus formation in Arabidopsis thaliana

  2020cited by this paper
• MYB94 and MYB96 additively inhibit callus formation via directly repressing LBD29 expression in Arabidopsis thaliana.

  2020cited by this paper
• Suppression of Metacaspase- and Autophagy-Dependent Cell Death Improves Stress-Induced Microspore Embryogenesis in Brassica napus

  2020cited by this paper
• Current Perspectives on the Auxin-Mediated Genetic Network that Controls the Induction of Somatic Embryogenesis in Plants

  2020cited by this paper
• A coherent feed-forward loop drives vascular regeneration in damaged aerial organs of plants growing in a normal developmental context

  2020cited by this paper
• Molecular Dissection of the Regenerative Capacity of Forest Tree Species: Special Focus on Conifers

  2019cited by this paper
• Histone acetylation orchestrates wound-induced transcriptional activation and cellular reprogramming in Arabidopsis

  2019cited by this paper
• AP2/ERF Transcription Factors Integrate Age and Wound Signals for Root Regeneration[OPEN]

  2019cited by this paper
• Gradient Expression of Transcription Factor Imposes a Boundary on Organ Regeneration Potential in Plants.

  2019cited by this paper
• Stress-Induced Microspore Embryogenesis Requires Endogenous Auxin Synthesis and Polar Transport in Barley

  2019cited by this paper
• Genome-Wide Reinforcement of DNA Methylation Occurs during Somatic Embryogenesis in Soybean

  2019cited by this paper
• Proteases with caspase 3-like activity participate in cell death during stress-induced microspore embryogenesis of Brassica napus

  2019cited by this paper
• Physcomitrella STEMIN transcription factor induces stem cell formation with epigenetic reprogramming

  2019cited by this paper
• Sphingolipid biosynthesis modulates plasmodesmal ultrastructure and phloem unloading

  2019cited by this paper
• The Four FAD-Dependent Histone Demethylases of Arabidopsis Are Differently Involved in the Control of Flowering Time

  2019cited by this paper
• An apical hypoxic niche sets the pace of shoot meristem activity

  2019cited by this paper
• Jasmonate-mediated wound signalling promotes plant regeneration

  2019cited by this paper
• Primed histone demethylation regulates shoot regenerative competency

  2019cited by this paper
• A Jasmonate Signaling Network Activates Root Stem Cells and Promotes Regeneration.

  2019cited by this paper
• Somatic Embryogenesis Induction in Woody Species: The Future After OMICs Data Assessment

  2019cited by this paper
• Molecular Mechanisms of Plant Regeneration.

  2019cited by this paper
• Microspore embryogenesis: targeting the determinant factors of stress-induced cell reprogramming for crop improvement.

  2019cited by this paper
• Signaling Overview of Plant Somatic Embryogenesis

  2019cited by this paper
• Endogenous Hypoxia in Lateral Root Primordia Controls Root Architecture by Antagonizing Auxin Signaling in Arabidopsis.

  2019cited by this paper
• Modulation of autophagy and protease activities by small bioactive compounds to reduce cell death and improve stress-induced microspore embryogenesis initiation in rapeseed and barley

  2019cited by this paper
• 5-Azacytidine: A Promoter of Epigenetic Changes in the Quest to Improve Plant Somatic Embryogenesis

  2018cited by this paper
• Epigenetic reprogramming by histone acetyltransferase HAG1/AtGCN5 is required for pluripotency acquisition in Arabidopsis

  2018cited by this paper
• The Arabidopsis LDL1/2-HDA6 histone modification complex is functionally associated with CCA1/LHY in regulation of circadian clock genes

  2018cited by this paper
• A Molecular Framework for Auxin-Controlled Homeostasis of Shoot Stem Cells in Arabidopsis.

  2018cited by this paper
• The WOX11–LBD16 Pathway Promotes Pluripotency Acquisition in Callus Cells During De Novo Shoot Regeneration in Tissue Culture

  2018cited by this paper
• JMJ30‐mediated demethylation of H3K9me3 drives tissue identity changes to promote callus formation in Arabidopsis

  2018cited by this paper
• Genome-Wide Identification of Arabidopsis LBD29 Target Genes Reveals the Molecular Events behind Auxin-Induced Cell Reprogramming during Callus Formation

  2018cited by this paper
• Dynamic Epigenetic Changes during Plant Regeneration.

  2018cited by this paper
• Control of auxin-induced callus formation by bZIP59–LBD complex in Arabidopsis regeneration

  2018cited by this paper
• Autophagy is activated and involved in cell death with participation of cathepsins during stress-induced microspore embryogenesis in barley

  2018cited by this paper
• Trichostatin A Triggers an Embryogenic Transition in Arabidopsis Explants via an Auxin-Related Pathway

  2018cited by this paper
• Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants

  2018cited by this paper
• H3K27 methylation: a promiscuous repressive chromatin mark.

  2017cited by this paper
• A comprehensive review of lysine-specific demethylase 1 and its roles in cancer.

  2017cited by this paper
• Inhibition of Histone H3K9 Methylation by BIX-01294 Promotes Stress-Induced Microspore Totipotency and Enhances Embryogenesis Initiation

  2017cited by this paper
• Azacytidine and miR156 promote rooting in adult but not in juvenile Arabidopsis tissues.

  2017cited by this paper
• A Two-Step Model for de Novo Activation of WUSCHEL during Plant Shoot Regeneration

  2017cited by this paper
• Arabidopsis ATXR2 deposits H3K36me3 at the promoters of LBD genes to facilitate cellular dedifferentiation

  2017cited by this paper
• Initiation of leaf somatic embryogenesis involves high pectin esterification, auxin accumulation and DNA demethylation in Quercus alba.

  2017cited by this paper
• The Arabidopsis ALF4 protein is a regulator of SCF E3 ligases

  2017cited by this paper
• Wounding Triggers Callus Formation via Dynamic Hormonal and Transcriptional Changes1[OPEN]

  2017cited by this paper
• A transcriptional view on somatic embryogenesis

  2017cited by this paper
• PCSD: a plant chromatin state database

  2017cited by this paper
• PRC2 Represses Hormone-Induced Somatic Embryogenesis in Vegetative Tissue of Arabidopsis thaliana

  2017cited by this paper
• Regulation of Hormonal Control, Cell Reprogramming, and Patterning during De Novo Root Organogenesis1[OPEN]

  2017cited by this paper
• Expression profiling of AUXIN RESPONSE FACTOR genes during somatic embryogenesis induction in Arabidopsis

  2017cited by this paper
• YUCCA-mediated auxin biogenesis is required for cell fate transition occurring during de novo root organogenesis in Arabidopsis

  2016cited by this paper
• Somatic Embryogenesis: Fundamental Aspects and Applications

  2016cited by this paper
• Wound signaling of regenerative cell reprogramming.

  2016cited by this paper
• Plant regeneration: cellular origins and molecular mechanisms

  2016cited by this paper
• Root Regeneration Triggers an Embryo-like Sequence Guided by Hormonal Interactions.

  2016cited by this paper
• WIND1 Promotes Shoot Regeneration through Transcriptional Activation of ENHANCER OF SHOOT REGENERATION1 in Arabidopsis[OPEN]

  2016cited by this paper
• Developmental Functions of miR156-Regulated SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) Genes in Arabidopsis thaliana

  2016cited by this paper
• Very-long-chain fatty acids restrict regeneration capacity by confining pericycle competence for callus formation in Arabidopsis

  2016cited by this paper
• Tug of war: adding and removing histone lysine methylation in Arabidopsis.

  2016cited by this paper
• Jasmonates: biosynthesis, metabolism, and signaling by proteins activating and repressing transcription

  2016cited by this paper
• The heterodimeric transcription factor complex ERF115–PAT1 grants regeneration competence

  2016cited by this paper
• 5-azacytidine promotes microspore embryogenesis initiation by decreasing global DNA methylation, but prevents subsequent embryo development in rapeseed and barley

  2015cited by this paper
• PLETHORA Genes Control Regeneration by a Two-Step Mechanism.

  2015cited by this paper
• An Intrinsic MicroRNA Timer Regulates Progressive Decline in Shoot Regenerative Capacity in Plants

  2015cited by this paper
• The role of chromatin modifications in somatic embryogenesis in plants

  2015cited by this paper
• Specific Membrane Lipid Composition Is Important for Plasmodesmata Function in Arabidopsis

  2015cited by this paper
• Arabidopsis histone demethylases LDL1 and LDL2 control primary seed dormancy by regulating DELAY OF GERMINATION 1 and ABA signaling-related genes

  2015cited by this paper
• Auxin Biosynthesis, Accumulation, Action and Transport are Involved in Stress-Induced Microspore Embryogenesis Initiation and Progression in Brassica napus.

  2015cited by this paper
• Group VII Ethylene Response Factors Coordinate Oxygen and Nitric Oxide Signal Transduction and Stress Responses in Plants1

  2015cited by this paper
• Somatic embryogenesis - Stress-induced remodeling of plant cell fate.

  2015cited by this paper
• Genetic and epigenetic controls of plant regeneration.

  2014cited by this paper
• Changes in Histone Methylation and Acetylation during Microspore Reprogramming to Embryogenesis Occur Concomitantly with BnHKMT and BnHAT Expression and Are Associated with Cell Totipotency, Proliferation, and Differentiation in Brassica napus

  2014cited by this paper
• Changes in DNA Methylation Levels and Nuclear Distribution Patterns after Microspore Reprogramming to Embryogenesis in Barley

  2014cited by this paper
• The Histone Deacetylase Inhibitor Trichostatin A Promotes Totipotency in the Male Gametophyte[W]

  2014cited by this paper
• Pioneer transcription factors in cell reprogramming

  2014cited by this paper
• Jumonji demethylases moderate precocious flowering at elevated temperature via regulation of FLC in Arabidopsis

  2014cited by this paper
• WOX11 and 12 Are Involved in the First-Step Cell Fate Transition during de Novo Root Organogenesis in Arabidopsis[W]

  2014cited by this paper
• CtBP and Associated LSD1 Are Required for Transcriptional Activation by NeuroD1 in Gastrointestinal Endocrine Cells

  2014cited by this paper
• Synthesis of Very-Long-Chain Fatty Acids in the Epidermis Controls Plant Organ Growth by Restricting Cell Proliferation

  2013cited by this paper
• ERF115 Controls Root Quiescent Center Cell Division and Stem Cell Replenishment

  2013cited by this paper
• Reprogramming of H3K27me3 Is Critical for Acquisition of Pluripotency from Cultured Arabidopsis Tissues

  2012cited by this paper
• LATERAL ORGAN BOUNDARIES DOMAIN transcription factors direct callus formation in Arabidopsis regeneration

  2012cited by this paper
• DNA methylation dynamics and MET1a-like gene expression changes during stress-induced pollen reprogramming to embryogenesis

  2012cited by this paper
• A new microspore embryogenesis system under low temperature which mimics zygotic embryogenesis initials, expresses auxin and efficiently regenerates doubled-haploid plants in Brassica napus

  2012cited by this paper
• Transcript profiling reveals complex auxin signalling pathway and transcription regulation involved in dedifferentiation and redifferentiation during somatic embryogenesis in cotton

  2012cited by this paper
• The AP2/ERF transcription factor WIND1 controls cell dedifferentiation in Arabidopsis.

  2011cited by this paper
• Epigenetics in plant development and response to stress.

  2011cited by this paper
• LSD1 regulates the balance between self-renewal and differentiation in human embryonic stem cells

  2011cited by this paper
• NO, ROS, and cell death associated with caspase-like activity increase in stress-induced microspore embryogenesis of barley

  2011cited by this paper
• Oxygen sensing in plants is mediated by an N-end rule pathway for protein destabilization

  2011cited by this paper
• Arabidopsis regeneration from multiple tissues occurs via a root development pathway.

  2010cited by this paper
• The Jumonji C Domain-Containing Protein JMJ30 Regulates Period Length in the Arabidopsis Circadian Clock1[W][OA]

  2010cited by this paper
• Oxygen in stem cell biology: a critical component of the stem cell niche.

  2010cited by this paper
• Members of the GCN5 Histone Acetyltransferase Complex Regulate PLETHORA-Mediated Root Stem Cell Niche Maintenance and Transit Amplifying Cell Proliferation in Arabidopsis[W]

  2009cited by this paper
• Organ regeneration does not require a functional stem cell niche in plants

  2008cited by this paper

• Dedifferentiation of Plant Cells: A Term Covering Multiple Pathways?

  2026cites this paper
• Efficient micropropagation of Argania spinosa through axillary budding

  2026cites this paper
• The transcription factor ESR2/DRNL/BOL orchestrates cytokinin dynamics leading to developmental reprogramming and green callus formation.

  2025cites this paper
• Impact of Light on In Vitro Plant Regeneration

  2025cites this paper
• Genetic control of plant morphogenesis in in vitro culture

  2025cites this paper
• Targeting histone acetylation to enhance somatic embryogenesis in Quercus suber L.

  2025cites this paper
• Analysing the loss of embryogenic competence in long-term cell lines of Solanum betaceum Cav.: involvement of miR827, phosphate and sugar

  2025cites this paper
• LEC2 unlocks totipotency by unlocking chromatin.

  2025cites this paper
• Tissue-Culture, Bulblet Regeneration, and Genetic Manipulation Systems in Lycoris

  2025cites this paper
• Development of an in vitro propagation system for ornamental Calathea fasciata ‘Borrusica’

  2025cites this paper
• Cold plasma as an efficient tool for enhancing callus induction, plant regeneration, and genetic transformation in safflower plant (Cartamus tinctorius)

  2025cites this paper
• Transcriptomic profiling reveals histone acetylation-regulated genes involved in somatic embryogenesis in Arabidopsis thaliana

  2024cites this paper
• Proteomic insights into adventitious root formation in Larix kaempferi.

  2024cites this paper
• Direct somatic embryogenesis on Coffea arabica L. explants previously subjected to water restriction

  2024influential citation
• Organogenesis as the Realization of the Morphogenetic Potential of Callus Cells in In Vitro Conditions

  2024cites this paper
• Small molecule inhibitors of human LRRK2 enhance in vitro embryogenesis and microcallus formation for plant regeneration of crop and model species.

  2024cites this paper
• A Comparison of DNA-Methylation during Protoplast Culture of Ponkan Mandarin (Citrus reticulata Blanco) and Tobacco (Nicotiana tabacum L.)

  2024cites this paper
• DNA demethylation by transitory 5‐azacytidine treatment improves somatic embryogenesis yield for regeneration and breeding of cork oak

  2024cites this paper
• Multiscale chromatin dynamics and high entropy in plant iPSC ancestors

  2024cites this paper
• Plant regeneration in the new era: from molecular mechanisms to biotechnology applications

  2024cites this paper
• ESR2 orchestrates cytokinin dynamics leading to developmental reprogramming and green callus formation

  2024cites this paper
• A Common Molecular Signature Indicates the Pre-Meristematic State of Plant Calli

  2023cites this paper
• Energetic regenerative medicine based on plant photosynthesis grafted human cells.

  2023cites this paper
• Regulatory roles of noncoding RNAs in callus induction and plant cell dedifferentiation

  2023cites this paper
• Multi-strategy approach towards optimization of maturation and germination in radiata pine somatic embryogenesis

  2023cites this paper
• Dynamics of Endogenous Auxin and Its Role in Somatic Embryogenesis Induction and Progression in Cork Oak

  2023cites this paper
• The inhibition of maize (Zea mays L.) root stem cell regeneration by low oxygen is attenuated by Phytoglobin 1 (Pgb1) through changes in auxin and jasmonic acid

  2023cites this paper
• Histological Approach to the Study of Morphogenesis in Callus Cultures In Vitro: A Review

  2023cites this paper
• H3K9 methylation patterns during somatic embryogenic competence expression in tamarillo (Solanum betaceum Cav.)

  2023cites this paper
• Thin Cell Layer Tissue Culture Technology with Emphasis on Tree Species

  2023cites this paper
• Opposite Auxin Dynamics Determine the Gametophytic and Embryogenic Fates of the Microspore

  2023cites this paper
• Transcriptomic Insight into the Pollen Tube Growth of Olea europaea L. subsp. europaea Reveals Reprogramming and Pollen-Specific Genes Including New Transcription Factors

  2023cites this paper
• Multiscale chromatin dynamics and high entropy in plant iPSC cultures

  2023cites this paper
• Evaluating Tartary Buckwheat Genotypes with High Callus Induction Rates and the Transcriptomic Profiling during Callus Formation

  2023cites this paper
• Rapid and Efficient Regeneration of Populus ussuriensis Kom. from Root Explants through Direct De Novo Shoot Organogenesis

  2022cites this paper
• Critical Stages of Cereal Embryogenesis: Theoretical and Practical Significance

  2022cites this paper
• Dynamic chromatin state profiling reveals regulatory roles of auxin and cytokinin in shoot regeneration.

  2022cites this paper
• Genetically engineered crops for sustainably enhanced food production systems

  2022cites this paper
• Propagation of “Valencia” orange (Citrus x sinensis Osbeck) by minigraft

  2022cites this paper
• Somatic Embryogenesis in Citrus (Citrus spp.), var. Valencia.

  2022cites this paper
• Secondary Somatic Embryogenesis in Centaurium erythraea Rafn

  2021influential citation
• Resetting of the 24-nt siRNA landscape in rice zygotes

  2021cites this paper
• Tissue-specific metabolic reprogramming during wound induced de novo organ formation in tomato hypocotyl explants

  2021cites this paper
• Postembryonic Organogenesis in Plants: Experimental Induction of New Shoot and Root Organs.

  2021cites this paper
• Dynamic Hormone Gradients Regulate Wound-Induced de novo Organ Formation in Tomato Hypocotyl Explants

  2021cites this paper
• Tissue-Specific Metabolic Reprogramming during Wound-Induced Organ Formation in Tomato Hypocotyl Explants

  2021cites this paper
• Epigenetics for Crop Improvement in Times of Global Change

  2021cites this paper
• Small molecule inhibitors of mammalian GSK-3β promote in vitro plant cell reprogramming and somatic embryogenesis in crop and forest species

  2021cites this paper
• Progress on medicinal plant regeneration and the road ahead

  2020cites this paper
• Suppression of Metacaspase- and Autophagy-Dependent Cell Death Improves Stress-Induced Microspore Embryogenesis in Brassica napus

  2020cites this paper