Carbonic anhydrases enhance activity of endogenous Na‐H exchangers and not the electrogenic Na/HCO3 cotransporter NBCe1‐A, expressed in Xenopus oocytes

According to the HCO3− metabolon hypothesis, direct association of cytosolic carbonic anhydrases (CAs) with the electrogenic Na/HCO3 cotransporter NBCe1‐A speeds transport by regenerating/consuming HCO3− . The present work addresses published discrepancies as to whether cytosolic CAs stimulate NBCe1‐A, heterologously expressed in Xenopus oocytes. We confirm the essential elements of the previous experimental observations, taken as support for the HCO3− metabolon hypothesis. However, using our own experimental protocols or those of others, we find that NBCe1‐A function is unaffected by cytosolic CAs. Previous conclusions that cytosolic CAs do stimulate NBCe1‐A can be explained by an unanticipated stimulatory effect of the CAs on an endogenous Na‐H exchanger. Theoretical analyses show that, although CAs could stimulate non‐ HCO3− transporters (e.g. Na‐H exchangers) by accelerating CO2/ HCO3− ‐mediated buffering of acid‐base equivalents, they could not appreciably affect transport rates of NBCe1 or other transporters carrying HCO3− , CO3= , or NaCO3− ion pairs.

• Publication year

  2020

• Venue

  Journal of Physiology

• Publication date

  2020-09-24

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1113/JP280143PMID 32969493PMCID PMC7747792
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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