Operational stability of copolymerized enzymes at elevated temperatures

No abstract is available for this paper.

• Publication year

  1983

• Venue

  Biotechnology and Bioengineering

• Publication date

  1983-08-01

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1002/bit.260250804PMID 18551540
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• [Inactivation and reactivation of proteins (enzymes)].

  1982cited by this paper
• Reactivation of enzymes irreversibly denatured at elevated temperature. Trypsin and alpha-chymotrypsin covalently immobilized on Sepharose 4B and in polyacrylamide gel.

  1980cited by this paper
• Reactivation of "irreversibly" denaturated enzymes.

  1980cited by this paper
• The principles of enzyme stabilization. IV. Modification of 'key' functional groups in the tertiary structure of proteins.

  1979cited by this paper
• Enzyme stabilization without carriers

  1979cited by this paper
• Manipulation of the properties of enzymes by covalent attachment of carbohydrate

  1978cited by this paper
• On the mechanism of irreversible thermoinactivation of enzymes and possibilities for reactivation of "irreversibly" inactivated enzymes.

  1978cited by this paper
• A rationale for stabilization of oxygen-labile enzymes: application to a clostridial hydrogenase.

  1978cited by this paper
• The principles of enzyme stabilization. I. Increase in thermostability of enzymes covalently bound to a complementary surface of a polymer support in a multipoint fashion.

  1977cited by this paper
• The principles of enzyme stabilization. II. Increase in the thermostability of enzymes as a result of multipoint noncovalent interaction with a polymeric support.

  1977cited by this paper
• Studies on conformation of soluble and immobilized enzymes using differential scanning calorimetry. 2. Specific activity and thermal stability of enzymes bound weakly and strongly to Sepharose CL 4B.

  1977cited by this paper
• [Basis principles of enzyme stabilization. Increase in alpha-chymotrypsin thermostability by covalent addition to the complementary surface of a polymeric carrier].

  1976cited by this paper
• Immobilized carboxypeptidase A as a probe for studying the thermally induced unfolding of bovine pancreatic ribonuclease.

  1975cited by this paper
• Sepharose-bound trypsin and activated sepharose-bound trypsinogen. Studies with small and large substrates.

  1974cited by this paper
• The chemical kinetics of enzyme action

  1973cited by this paper
• The denaturation by urea and guanidinium chloride of trypsin and N-acetylated-trypsin derivatives bound to Sephadex and agarose.

  1973cited by this paper
• Immobilized glucoamylase on porous glass

  1973cited by this paper
• Studies on immobilized enzymes. X. Preparation and properties of aminoacylase entrapped into acrylamide gel-lattice.

  1972cited by this paper
• Studies on immobilized enzymes. IX. Preparation and properties of aminoacylase covalently attached to halogenoacetylcelluloses.

  1971cited by this paper
• Changes in conformation of insolubilized trypsin and chymotrypsin, followed by fluorescence.

  1971cited by this paper
• Some properties of a bacterial proteinase chemically fixed to agarose.

  1970cited by this paper
• Water-insoluble enzymes: kinetics of rabbit muscle enolase embedded within an insoluble carrier.

  1969cited by this paper
• Protein denaturation.

  1968cited by this paper
• The Chemical Kinetics of Enzyme Action.

  1959cited by this paper

• Chemically Bonded Pepsin via Its Inert Center to Diazo Functionalized Silica Gel through Multipoint Attachment Mode: A Way of Restoring Biocatalytic Sustainability over "Wider pH" Range.

  2024cites this paper
• Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies.

  2021cites this paper
• Construction of Enzyme Nanoreactors to Enable Tumor Microenvironment Modulation and Enhanced Cancer Treatment

  2020cites this paper
• Multi-Point Covalent Immobilization of Enzymes on Supports Activated with Epoxy Groups: Stabilization of Industrial Enzymes.

  2020cites this paper
• Production and optimization of isopropyl palmitate via biocatalytic route using home-made enzymatic catalysts

  2018cites this paper
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  2017cites this paper
• RESOLUCION CINETICA DE (R/S)-PROPANOLOL Y (R/S)-ATENOLOL VIA REACCIONES DE ACETILACION MEDIANTE LA UTILIZACION DE LIPASAS INMOVILIZADAS

  2013cites this paper
• Parameters in preparation and characterization of cross linked enzyme aggregates (CLEAs)

  2013cites this paper
• Coupling Chemical Modification and Immobilization to Improve the Catalytic Performance of Enzymes

  2011cites this paper
• Synthesis and characterization of thermo-responsive poly(N-isopropylacrylamide)-bovine liver catalase bioconjugate

  2010cites this paper
• l-Dopa synthesis using tyrosinase immobilized on magnetic beads

  2009cites this paper
• Synthesis and characterization of cyclodextrin-based polymers as a support for immobilization of Candida rugosa lipase

  2009cites this paper
• Improving the properties of chitosan as support for the covalent multipoint immobilization of chymotrypsin.

  2008cites this paper
• Multipoint covalent immobilization of microbial lipase on chitosan and agarose activated by different methods

  2008cites this paper
• Improvement of enzyme activity, stability and selectivity via immobilization techniques

  2007influential citation
• Immobilization of enzymes: an approach to fundamental studies in biochemistry.

  2006cites this paper
• Immobilization-Stabilization of Enzymes by Multipoint Covalent Attachment on Supports Activated With Epoxy Groups

  2006cites this paper
• Preparation of an Industrial Biocatalyst of Penicillin G Acylase on Sepabeads

  2005cites this paper
• Immobilization of Enzymes by Covalent Attachment

  2005cites this paper
• The use of chemically stabilised proteolytic enzymes in peptide synthesis

  2003cites this paper
• Hydrolysis of Proteins by Immobilized‐Stabilized Alcalase‐Glyoxyl Agarose

  2003cites this paper
• Preparation of a Stable Biocatalyst of Bovine Liver Catalase Using Immobilization and Postimmobilization Techniques

  2003cites this paper
• Epoxy Sepabeads: A Novel Epoxy Support for Stabilization of Industrial Enzymes via Very Intense Multipoint Covalent Attachment

  2002cites this paper
• Preparation of artificial hyper-hydrophilic micro-environments (polymeric salts) surrounding enzyme molecules: New enzyme derivatives to be used in any reaction medium

  2002cites this paper
• Enzyme conjugation to the polysaccharide chitosan: smart biocatalysts and biocatalytic hydrogels.

  2001cites this paper
• Stabilization of Immobilized Enzymes Against Water-Soluble Organic Cosolvents and Generation of Hyper-Hydrophilic Micro-Environments Surrounding Enzyme Molecules

  2001influential citation
• Immobilization of functionally unstable catechol-2,3-dioxygenase greatly improves operational stability.

  2000cites this paper
• Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment*

  2000cites this paper
• Immobilization of Enzymes on Glyoxyl Agarose

  1997cites this paper
• Activity and stability of native and modified alanine aminotransferase in cosolvent systems and denaturants

  1997cites this paper
• A fusion protein designed for noncovalent immobilization: stability, enzymatic activity, and use in an enzyme reactor

  1996cites this paper
• Improved Refolding of a Matrix‐Bound Fusion Protein

  1996cites this paper
• Application of Materials in Medicine, Biology, and Artificial Organs

  1996cites this paper
• Modulation of Activity/Stability Properties of Lipase from Pseudomonas Flourescens by Multipoint Covalent Immobilization on Glyoxyl-Supports

  1996cites this paper
• Utilization of Unfolding/Refolding Strategies for Reactivation of Immobilized Derivatives of Lipases after Inactivation by Organic Solvents

  1996cites this paper
• Chemical modification of bovine trypsin for use in peptide synthesis

  1996influential citation
• Hyperstabilization of a thermophilic esterase by multipoint covalent attachment

  1995cites this paper
• Stability of native and covalently modified papain.

  1995cites this paper
• Strategies for enzyme stabilization by intramolecular crosslinking with bifunctional reagents

  1995cites this paper
• Thermal stability of immobilized α-chymotrypsin is additionally increased by chaotropic compounds

  1995cites this paper
• Reversible conformational transition gives rise to 'zig-zag' temperature dependence of the rate constant of irreversible thermoinactivation of enzymes.

  1994cites this paper
• Correlation of high-temperature stability of alpha-chymotrypsin with 'salting-in' properties of solution.

  1994cites this paper
• Enzyme Stabilization by Multipoint Covalent Attachment to Activated Pre-Existing Supports

  1993cites this paper
• Mechanism-based strategies for protein thermostabilization.

  1993cites this paper
• Stabilization of Micrococcal Endonuclease by Immobilization on Agarose Gels Highly Activated with CNBr

  1993cites this paper
• Stabilization of proteins by chemical methods

  1993cites this paper
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  1992cites this paper
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  1992cites this paper
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  1992cites this paper
• Enzyme stabilization: state of the art

  1991cites this paper
• Functionally-stabilized proteins--a review.

  1991cites this paper
• Homogeneous solutions of hydrophilic enzymes in nonpolar organic solvents New systems for fundamental studies and biocatalytic transformations

  1991cites this paper
• Immobilization-stabilization of alpha-chymotrypsin by covalent attachment to aldehyde-agarose gels.

  1991cites this paper
• Immobilization‐stabilization of α‐chymotrypsin by covalent attachment to aldehyde‐agarose gels

  1991cites this paper
• Immobilization provides additional stabilization of an initially stable protease from Thermoactinomyces vulgaris (thermitase)

  1990cites this paper
• Structure-stability relationships in proteins: a guide to approaches to stabilizing enzymes

  1990cites this paper
• Strategy for Stabilizing Enzymes Part One: Increasing Stability of Enzymes via their Multi-Point Interaction with a Support

  1990cites this paper
• Strategy for Stabilizing Enzymes Part Two: Increasing Enzyme Stability by Selective Chemical Modification

  1990cites this paper
• Multipoint attachment to a support protects enzyme from inactivation by organic solvents: α‐Chymotrypsin in aqueous solutions of alcohols and diols

  1990cites this paper
• [Stability and stabilization of enzymes].

  1989cites this paper
• Enzyme deactivation.

  1988cites this paper
• Protecting effect of competitive inhibitors during very intense insolubilized enzyme-activated support multipoint attachments: trypsin (amine)-agarose (aldehyde) system

  1988cites this paper
• Aldehyde-agarose gels as activated supports for immobilization-stabilization of enzymes

  1988cites this paper
• Engineering biocatalytic systems in organic media with low water content

  1988cites this paper
• [53] Enzyme stabilization by immobilization

  1988cites this paper
• Protein stabilization via hydrophilization

  1988cites this paper
• Effect of serum albumin on the structure of polyacrylamide hydrogels

  1988cites this paper
• Structure-stability relationship in proteins: fundamental tasks and strategy for the development of stabilized enzyme catalysts for biotechnology.

  1988cites this paper
• Protein stabilization via hydrophilization. Covalent modification of trypsin and alpha-chymotrypsin.

  1988cites this paper
• Coaggregate of trypsin and chymotrypsin

  1988cites this paper
• Enzyme stabilization by immobilization.

  1988cites this paper
• Stabilization of Trypsin by Multiple‐Point Attachment to Aldehyde‐Agarose Gels

  1987cites this paper
• Enzymes Entrapped Into Reversed Micelles Of Surfactants In Organic Solvents: Key Trends In Applied Enzymology (Biotechnology)

  1987cites this paper
• Reactivation of immobilized enzymes.

  1987cites this paper
• [53] Reactivation of immobilized enzymes

  1987cites this paper
• Heterogeneous denaturation of enzymes: A distributed activation energy model with nonuniform activities

  1985cites this paper
• Synthesis and polymerization of macromonomers based on physiologically active compounds. Review

  1985cites this paper
• Immobilization of a soluble chemically thermostabilized enzyme

  1985cites this paper
• Structure-stability relationships in proteins: new approaches to stabilizing enzymes

  1984cites this paper
• Catalysis by Enzymes Entrapped in Reversed Micelles of Surfactants in Organic Solvents

  1984cites this paper
• Precipitation, chelation, and the availability of metals as nutrients in anaerobic digestion. II. Applications

  1983cites this paper