Epoxy Sepabeads: A Novel Epoxy Support for Stabilization of Industrial Enzymes via Very Intense Multipoint Covalent Attachment

No abstract is available for this paper.

• Publication year

  2002

• Venue

  Biotechnology progress (Print)

• Publication date

  Unknown publication date

• Fields of study

  Medicine, Materials Science, Chemistry, Engineering

• Identifiers
  DOI 10.1021/bp010171nPMID 12052083
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2000cited by this paper
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  2000cited by this paper
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  1999cited by this paper
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  1995cited by this paper
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  1993cited by this paper
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  1993cited by this paper
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  1993cited by this paper
• Immobilized nucleases.

  1993cited by this paper
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  1991cited by this paper
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  1991cited by this paper
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  1991cited by this paper
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  1990cited by this paper
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  1986cited by this paper
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  1985cited by this paper
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  1984cited by this paper
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  1983cited by this paper
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  1983cited by this paper
• Immobilized Enzymes and Cells as Practical Catalysts

  1983cited by this paper
• [Immobilized enzymes and cells].

  1975cited by this paper

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