Recent advancements of human iPSC derived cardiomyocytes in drug screening and tissue regeneration

Myocardial infarction together with subsequent heart failures are among the main reasons for death related to cardiovascular diseases (CVD). Restoring cardiac function and replacing scar tissue with healthy regenerated cardiomyocytes (CMs) is a hopeful therapy for heart failure. Human-induced pluripotent stem cell (hiPSC) derived CMs (hiPSC-CMs) offer the advantages of not having significant ethical issues and having negligible immunological rejection compared to other myocardial regeneration methods. hiPSCs can also produce an unlimited number of human CMs, another advantage they have compared with other cell sources for cardiac regeneration. Numerous researchers have focused their work on promoting the functional maturity of hiPSC-CMs, as well as finding out the precise regulatory mechanisms of each differentiation stage together with the economical and practical methods of acquisition and purification. However, the clinical applications of hiPSC-CMs in drug discovery and cardiac regeneration therapy have yet to be achieved. In this review, we present an overview of various methods for improving the differentiation efficiency of hiPSC-CMs and discuss the differences of electrophysiological characteristics between hiPSC-CMs and matured native CMs. We also introduce approaches for obtaining a large quantity of iPSC-CMs, which are needed to achieve biomanufacturing strategies for building biomimetic three-dimensional tissue constructs using combinations of biomaterials and advanced microfabrication techniques. Recent advances in specific iPSC technology-based drug screening platforms and regeneration therapies can suggest future directions for personalized medicine in biomedical applications.

• Publication year

  2020

• Venue

  Microphysiological Systems

• Publication date

  2020-09-01

• Fields of study

  Medicine

• Identifiers
  DOI 10.21037/mps-20-3PMID 39430371PMCID 11488690
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Engineering microenvironment for human cardiac tissue assembly in heart-on-a-chip platform.

  2020cited by this paper
• Nanotechnological strategies for engineering complex tissues.

  2020cited by this paper
• MAP4K4 Inhibition Promotes Survival of Human Stem Cell-Derived Cardiomyocytes and Reduces Infarct Size In Vivo

  2020cited by this paper
• Bioengineering 3D Cardiac Microtissues Using Bioassembly

  2019cited by this paper
• Human iPSC banking: barriers and opportunities

  2019cited by this paper
• Biomimetic design and fabrication of scaffolds integrating oriented micro-pores with branched channel networks for myocardial tissue engineering

  2019cited by this paper
• Assessment of Proarrhythmic Potential of Drugs in Optogenetically Paced Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

  2019cited by this paper
• Stars in the Night Sky: iPSC-Cardiomyocytes Return the Patient Context to Drug Screening.

  2019influential reference
• Attenuation of doxorubicin-induced cardiotoxicity in a human in vitro cardiac model by the induction of the NRF-2 pathway.

  2019cited by this paper
• Human Induced Pluripotent Stem-Cardiac-Endothelial-Tumor-on-a-Chip to Assess Anticancer Efficacy and Cardiotoxicity

  2019cited by this paper
• Concise Review: Precision Matchmaking: Induced Pluripotent Stem Cells Meet Cardio‐Oncology

  2019cited by this paper
• 3D Printing of Personalized Thick and Perfusable Cardiac Patches and Hearts

  2019cited by this paper
• MAP4K4 Inhibition Promotes Survival of Human Stem Cell-Derived Cardiomyocytes and Reduces Infarct Size In Vivo

  2019cited by this paper
• Human-Induced Pluripotent Stem Cell Model of Trastuzumab-Induced Cardiac Dysfunction in Patients With Breast Cancer.

  2019cited by this paper
• Polyethylene Terephthalate Textiles Enhance the Structural Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

  2019cited by this paper
• Transcriptomic analysis of 3D Cardiac Differentiation of Human Induced Pluripotent Stem Cells Reveals Faster Cardiomyocyte Maturation Compared to 2D Culture

  2019cited by this paper
• Personalized medicine in cardio-oncology: the role of induced pluripotent stem cell

  2019cited by this paper
• Polymer Microparticles with Defined Surface Chemistry and Topography Mediate the Formation of Stem Cell Aggregates and Cardiomyocyte Function.

  2019cited by this paper
• Proarrhythmia risk prediction using human induced pluripotent stem cell-derived cardiomyocytes.

  2018cited by this paper
• Folic Acid Derived Hydrogel Enhances the Survival and Promotes Therapeutic Efficacy of iPS Cells for Acute Myocardial Infarction.

  2018cited by this paper
• Acellular therapeutic approach for heart failure: in vitro production of extracellular vesicles from human cardiovascular progenitors

  2018cited by this paper
• Organs-on-a-Chip: A Fast Track for Engineered Human Tissues in Drug Development.

  2018cited by this paper
• Contractile Work Contributes to Maturation of Energy Metabolism in hiPSC-Derived Cardiomyocytes

  2018cited by this paper
• Modeling human diseases with induced pluripotent stem cells: from 2D to 3D and beyond

  2018cited by this paper
• Efficient differentiation of cardiomyocytes and generation of calcium-sensor reporter lines from nonhuman primate iPSCs

  2018cited by this paper
• Cardiac recovery via extended cell-free delivery of extracellular vesicles secreted by cardiomyocytes derived from induced pluripotent stem cells

  2018cited by this paper
• Potential applications of three-dimensional structure of silk fibroin/poly(ester-urethane) urea nanofibrous scaffold in heart valve tissue engineering

  2018cited by this paper
• Towards an autologous iPSC-derived patient-on-a-chip

  2018cited by this paper
• Heart repair via cardiomyocyte-secreted vesicles

  2018cited by this paper
• Development of a Human iPSC Cardiomyocyte-Based Scoring System for Cardiac Hazard Identification in Early Drug Safety De-risking

  2018cited by this paper
• Perspectives on the Use of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Biomedical Research

  2018cited by this paper
• Cardiac Regeneration with Human Pluripotent Stem Cell-Derived Cardiomyocytes

  2018cited by this paper
• International Multisite Study of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes for Drug Proarrhythmic Potential Assessment

  2018influential reference
• Will iPSC‐cardiomyocytes revolutionize the discovery of drugs for heart disease?

  2018cited by this paper
• Coordinated Proliferation and Differentiation of Human-Induced Pluripotent Stem Cell-Derived Cardiac Progenitor Cells Depend on Bone Morphogenetic Protein Signaling Regulation by GREMLIN 2

  2017cited by this paper
• Electrospun conductive nanofibrous scaffolds for engineering cardiac tissue and 3D bioactuators.

  2017cited by this paper
• Extrusion Bioprinting of Shear‐Thinning Gelatin Methacryloyl Bioinks

  2017cited by this paper
• Bioacoustic-enabled patterning of human iPSC-derived cardiomyocytes into 3D cardiac tissue.

  2017cited by this paper
• Transplantation of purified iPSC-derived cardiomyocytes in myocardial infarction

  2017cited by this paper
• Engineering a functional three-dimensional human cardiac tissue model for drug toxicity screening

  2017cited by this paper
• MHC matching improves engraftment of iPSC-derived neurons in non-human primates

  2017cited by this paper
• Gelatin electrospun nanofibrous matrices for cardiac tissue engineering applications

  2017cited by this paper
• Human iPSC-derived cardiomyocytes and tissue engineering strategies for disease modeling and drug screening.

  2017cited by this paper
• MHC matching improves engraftment of iPSC-derived neurons in non-human primates

  2017cited by this paper
• Human iPSC-derived cardiomyocytes cultured in 3D engineered heart tissue show physiological upstroke velocity and sodium current density

  2017influential reference
• Contribution of two-pore K+ channels to cardiac ventricular action potential revealed using human iPSC-derived cardiomyocytes.

  2017cited by this paper
• Bioengineering Approaches to Mature Human Pluripotent Stem Cell-Derived Cardiomyocytes

  2017cited by this paper
• Composite poly(lactic acid)/chitosan nanofibrous scaffolds for cardiac tissue engineering.

  2017cited by this paper
• Subtype-specific differentiation of cardiac pacemaker cell clusters from human induced pluripotent stem cells

  2017cited by this paper
• Comprehensive Translational Assessment of Human-Induced Pluripotent Stem Cell Derived Cardiomyocytes for Evaluating Drug-Induced Arrhythmias

  2017cited by this paper
• Instrumented cardiac microphysiological devices via multi-material 3D printing

  2016cited by this paper
• Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts

  2016cited by this paper
• Human induced pluripotent stem cell–derived cardiomyocytes recapitulate the predilection of breast cancer patients to doxorubicin-induced cardiotoxicity

  2016cited by this paper
• Microfluidic Bioprinting of Heterogeneous 3D Tissue Constructs Using Low‐Viscosity Bioink

  2016cited by this paper
• Human iPS Cell-Derived Cardiac Tissue Sheets: a Platform for Cardiac Regeneration

  2016cited by this paper
• Subtype-specific promoter-driven action potential imaging for precise disease modelling and drug testing in hiPSC-derived cardiomyocytes

  2016cited by this paper
• Translation of Human-Induced Pluripotent Stem Cells: From Clinical Trial in a Dish to Precision Medicine.

  2016cited by this paper
• Reduced Graphene Oxide-GelMA Hybrid Hydrogels as Scaffolds for Cardiac Tissue Engineering.

  2016cited by this paper
• Transcriptome Profiling of Patient-Specific Human iPSC-Cardiomyocytes Predicts Individual Drug Safety and Efficacy Responses In Vitro.

  2016cited by this paper
• Myocardial commitment from human pluripotent stem cells: Rapid production of human heart grafts.

  2016cited by this paper
• Cardiovascular Organ-on-a-Chip Platforms for Drug Discovery and Development

  2016cited by this paper
• Gelatin-Methacryloyl Hydrogels: Towards Biofabrication-Based Tissue Repair.

  2016cited by this paper
• Electrical and mechanical stimulation of cardiac cells and tissue constructs.

  2016cited by this paper
• The myocardial regenerative potential of three-dimensional engineered cardiac tissues composed of multiple human iPS cell-derived cardiovascular cell lineages

  2016cited by this paper
• Bioengineering Human Myocardium on Native Extracellular Matrix.

  2016cited by this paper
• High throughput physiological screening of iPSC-derived cardiomyocytes for drug development.

  2016cited by this paper
• Bi-layered polyurethane - Extracellular matrix cardiac patch improves ischemic ventricular wall remodeling in a rat model.

  2016cited by this paper
• Instrumented cardiac microphysiological devices via multimaterial three-dimensional printing

  2016cited by this paper
• Current advances and future perspectives in extrusion-based bioprinting.

  2016cited by this paper
• Miniaturized iPS-Cell-Derived Cardiac Muscles for Physiologically Relevant Drug Response Analyses

  2016influential reference
• Validating the pharmacogenomics of chemotherapy-induced cardiotoxicity: What is missing?

  2016cited by this paper
• Patient-Specific and Genome-Edited Induced Pluripotent Stem Cell-Derived Cardiomyocytes Elucidate Single-Cell Phenotype of Brugada Syndrome.

  2016cited by this paper
• Human induced pluripotent stem cell-derived cardiomyocytes: insights into molecular, cellular, and functional phenotypes.

  2015cited by this paper
• Molecular beacon-based detection and isolation of working-type cardiomyocytes derived from human pluripotent stem cells.

  2015cited by this paper
• Induced Pluripotent Stem Cell–Derived Cardiomyocytes: A New and Versatile Human In Vitro Cardiomyocyte Model Human Induced Pluripotent Stem Cell–Derived Cardiomyocytes Insights Into Molecular, Cellular, and Functional Phenotypes

  2015cited by this paper
• Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells Using Monolayer Culture

  2015cited by this paper
• Peptide Bioink: Self-Assembling Nanofibrous Scaffolds for Three-Dimensional Organotypic Cultures.

  2015cited by this paper
• Modelling sarcomeric cardiomyopathies in the dish: from human heart samples to iPSC cardiomyocytes

  2015cited by this paper
• From cardiac tissue engineering to heart-on-a-chip: beating challenges

  2015cited by this paper
• Human iPSC-based Cardiac Microphysiological System For Drug Screening Applications

  2015cited by this paper
• Cell Based Regenerative Therapy of Heart Failure: Where Do We Stand?

  2015cited by this paper
• Atrial-like cardiomyocytes from human pluripotent stem cells are a robust preclinical model for assessing atrial-selective pharmacology

  2015cited by this paper
• Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels.

  2015cited by this paper
• Combining Hypoxia and Bioreactor Hydrodynamics Boosts Induced Pluripotent Stem Cell Differentiation Towards Cardiomyocytes

  2014cited by this paper
• Human Embryonic Stem Cell-Derived Cardiomyocytes Regenerate Non-Human Primate Hearts

  2014cited by this paper
• Creating perfused functional vascular channels using 3D bio-printing technology.

  2014cited by this paper
• An intermittent rocking platform for integrated expansion and differentiation of human pluripotent stem cells to cardiomyocytes in suspended microcarrier cultures.

  2014cited by this paper
• The 3D printing of gelatin methacrylamide cell-laden tissue-engineered constructs with high cell viability.

  2014cited by this paper
• Controlling Expansion and Cardiomyogenic Differentiation of Human Pluripotent Stem Cells in Scalable Suspension Culture

  2014cited by this paper
• Direct-write bioprinting of cell-laden methacrylated gelatin hydrogels

  2014cited by this paper
• Tri-iodo-l-thyronine promotes the maturation of human cardiomyocytes-derived from induced pluripotent stem cells.

  2014cited by this paper
• 3D bioprinting of tissues and organs

  2014cited by this paper
• Inhibition of stearoyl-coA desaturase selectively eliminates tumorigenic Nanog-positive cells: Improving the safety of iPS cell transplantation to myocardium

  2014cited by this paper
• Chemically defined generation of human cardiomyocytes

  2014cited by this paper
• Cardiac repair in a porcine model of acute myocardial infarction with human induced pluripotent stem cell-derived cardiovascular cells.

  2014cited by this paper
• Disease modeling and phenotypic drug screening for diabetic cardiomyopathy using human induced pluripotent stem cells.

  2014influential reference
• Mesenchymal Stem Cells and Their Conditioned Medium Improve Integration of Purified Induced Pluripotent Stem Cell–Derived Cardiomyocyte Clusters into Myocardial Tissue

  2014cited by this paper
• Generating a Non-Integrating Human Induced Pluripotent Stem Cell Bank from Urine-Derived Cells

  2013cited by this paper
• Concise Review: Maturation Phases of Human Pluripotent Stem Cell‐Derived Cardiomyocytes

  2013cited by this paper
• Structural and Functional Maturation of Cardiomyocytes Derived from Human Pluripotent Stem Cells

  2013cited by this paper

• Prediction of cardiac differentiation in human induced pluripotent stem cell-derived cardiomyocyte supernatant using surface-enhanced Raman spectroscopy and machine learning.

  2025cites this paper
• Cell-based regenerative and rejuvenation strategies for treating neurodegenerative diseases

  2025cites this paper
• Induced Pluripotent Stem Cells-Based Regenerative Therapies in Treating Human Aging-Related Functional Decline and Diseases

  2025cites this paper
• Innovations in cardiac regenerative medicine: The role of tissue engineering

  2025cites this paper
• SarcGraph for High-Throughput Regional Analysis of Sarcomere Organization and Contractile Function in 2D Cardiac Muscle Bundles

  2025cites this paper
• Organ-Specific Strategies in Bioprinting: Addressing Translational Challenges in the Heart, Liver, Kidney, and Pancreas

  2025cites this paper
• Validation of a functional human AD model with four AD therapeutics utilizing patterned ipsc-derived cortical neurons integrated with microelectrode arrays

  2024cites this paper
• Validation of a functional human AD model with four AD therapeutics utilizing patterned iPSC-derived cortical neurons integrated with microelectrode arrays

  2024cites this paper
• Toward the latest advancements in cardiac regeneration using induced pluripotent stem cells (iPSCs) technology: approaches and challenges

  2024cites this paper
• Detecting and measuring of GPCR signaling – comparison of human induced pluripotent stem cells and immortal cell lines

  2023cites this paper
• Human in vitro neurocardiac coculture (iv NCC) assay development for evaluating cardiac contractility modulation

  2022cites this paper
• Electrospun piezoelectric scaffolds for cardiac tissue engineering.

  2022cites this paper
• Facile Bioprinting Process for Fabricating Size‐Controllable Functional Microtissues Using Light‐Activated Decellularized Extracellular Matrix‐Based Bioinks

  2021cites this paper