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An ICT-based fluorescent probe for ratiometric monitoring the fluctuations of peroxynitrite in mitochondria

Jie Cui,Shunping Zang,Hailiang Nie,Tiaojiao Shen,Sa Su,Jing Jing,Xiaoling Zhang

Published 2021 in Sensors and Actuators B-chemical

ABSTRACT

Abstract Peroxynitrite (ONOO−) is a well-known reactive oxygen species (ROS) which closely involves in various physiological and pathological processes. It is reported that mitochondria are the major site of ONOO− production. Normal level of ONOO− benefits mitochondria biological functions, while its overproduction will induce oxidative stress, resulting in an imbalance of mitochondria homeostasis. Therefore, accurate monitoring of ONOO− in mitochondria will be beneficial for elucidating the physiological metabolism and fluctuations of ONOO−. Herein, a novel mitochondria-targeted ratiometric fluorescent probe Mito-NA for ONOO− was designed rationally by regulating its intramolecular charge transfer (ICT) effect. Once reaction with ONOO−, Mito-NA manifested remarkable ratiometric fluorescence changes (18-fold) with a large red-shift (∼104 nm) emission, which was ascribed to the enhanced ICT process caused by the elimination of the electron-withdrawing recognition group 3-(trifluoromethyl) cinnamic acid and the exposure of electron-donating hydroxyl on Mito-NA structure. Furthermore, Mito-NA exhibited satisfactory performances for ONOO− such as good sensitivity (LOD =0.12 μM), high selectivity to ONOO− over other ROS, fast response and good mitochondrial targeting. More importantly, Mito-NA was successfully applied for ratiometric imaging and monitoring the fluctuations of mitochondrial ONOO− in living cells.

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