A highly selective fluorescent probe for the detection and imaging of peroxynitrite in living cells.

We have found a specific reaction between ketone 1 and peroxynitrite (ONOO-), rather than other reactive oxygen species and reactive nitrogen species generated in the biological system. On the basis of this reaction, we have successfully developed a new fluorescent probe HKGreen-1, which is highly selective for the detection of peroxynitrite in living cells. Before the oxidation with peroxynitrite, the dichlorofluorescein part is masked and the probe is nonfluorescent. However, upon reaction with peroxynitrite, the fluorophore is released, resulting in strong enhancement in fluorescence intensity.

• Publication year

  2006

• Venue

  Journal of the American Chemical Society

• Publication date

  2006-04-14

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1021/JA0603756PMID 16669647
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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