Rapid discovery of potent siRNA-containing lipid nanoparticles enabled by controlled microfluidic formulation.

No abstract is available for this paper.

• Publication year

  2012

• Venue

  Journal of the American Chemical Society

• Publication date

  2012-04-10

• Fields of study

  Medicine, Materials Science, Chemistry, Engineering

• Identifiers
  DOI 10.1021/ja301621zPMID 22475086
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• In vivo delivery of RNAi with lipid-based nanoparticles.

  2011cited by this paper
• Non-viral Delivery of Inductive and Suppressive Genes to Adipose-Derived Stem Cells for Osteogenic Differentiation

  2011cited by this paper
• Synthesis of Size‐Tunable Polymeric Nanoparticles Enabled by 3D Hydrodynamic Flow Focusing in Single‐Layer Microchannels

  2011cited by this paper
• Microfluidic assembly of lipid-based oligonucleotide nanoparticles.

  2011cited by this paper
• Multiplexed supramolecular self-assembly for non-viral gene delivery.

  2010cited by this paper
• Microfluidic mixing and the formation of nanoscale lipid vesicles.

  2010cited by this paper
• Targeted Delivery of RNAi Therapeutics With Endogenous and Exogenous Ligand-Based Mechanisms.

  2010cited by this paper
• Targeted delivery of RNAi therapeutics with endogenous and exogenous ligand-based mechanisms.

  2010cited by this paper
• Effects of temperature, acyl chain length, and flow-rate ratio on liposome formation and size in a microfluidic hydrodynamic focusing device

  2010cited by this paper
• Rational design of cationic lipids for siRNA delivery

  2010cited by this paper
• Delivery of antisense oligodeoxyribonucleotide lipopolyplex nanoparticles assembled by microfluidic hydrodynamic focusing.

  2010cited by this paper
• Single-step assembly of homogenous lipid-polymeric and lipid-quantum dot nanoparticles enabled by microfluidic rapid mixing.

  2010cited by this paper
• Lipid-like materials for low-dose, in vivo gene silencing

  2010cited by this paper
• Combinatorial evaluation of cations, pH-sensitive and hydrophobic moieties for polymeric vector design.

  2009cited by this paper
• Development of lipidoid-siRNA formulations for systemic delivery to the liver.

  2009cited by this paper
• Coaxial electrohydrodynamic spraying: a novel one-step technique to prepare oligodeoxynucleotide encapsulated lipoplex nanoparticles.

  2009cited by this paper
• Knocking down barriers: advances in siRNA delivery

  2009cited by this paper
• A combinatorial library of lipid-like materials for delivery of RNAi therapeutics

  2008cited by this paper
• Microfluidic platform for controlled synthesis of polymeric nanoparticles.

  2008cited by this paper
• RNAi therapeutics: a potential new class of pharmaceutical drugs

  2006cited by this paper
• Cationic lipid saturation influences intracellular delivery of encapsulated nucleic acids.

  2005cited by this paper
• A Scalable, Extrusion-Free Method for Efficient Liposomal Encapsulation of Plasmid DNA

  2005cited by this paper
• Recent advances with liposomes as pharmaceutical carriers

  2005cited by this paper
• Controlled vesicle self-assembly in microfluidic channels with hydrodynamic focusing.

  2004cited by this paper
• Experimental test of scaling of mixing by chaotic advection in droplets moving through microfluidic channels.

  2003cited by this paper
• Chaotic Mixer for Microchannels

  2002cited by this paper
• Spontaneous entrapment of polynucleotides upon electrostatic interaction with ethanol-destabilized cationic liposomes.

  2001cited by this paper
• Efficient encapsulation of antisense oligonucleotides in lipid vesicles using ionizable aminolipids: formation of novel small multilamellar vesicle structures.

  2001cited by this paper
• Fabrication of microfluidic systems in poly(dimethylsiloxane)

  2000cited by this paper
• Stabilized plasmid-lipid particles: construction and characterization

  1999cited by this paper
• Improved DNA: liposome complexes for increased systemic delivery and gene expression

  1997cited by this paper
• The mechanism of vesicle formation.

  1988cited by this paper
• Vesicles of variable sizes produced by a rapid extrusion procedure.

  1986cited by this paper
• Multiplexed Supramolecular Self-assembly for Non-viral Gene Delivery

  year unknowncited by this paper

• High‐Throughput In Vivo Screening Using Barcoded mRNA Identifies Lipid Nanoparticles With Extrahepatic Tropism for In Situ Immunoengineering

  2026cites this paper
• One-step nanoprecipitation of polymeric nanoparticles with tunable size, uniformity, shape and surface function

  2026cites this paper
• LNP-Mediated CF10 Delivery Selectively Enhances Potency to Colorectal Cancer Cells and Preserves the TS/Top1 Dual Targeting Mechanism.

  2026cites this paper
• Lipid-Polymer Nanoparticles (LiPoNs) Mediated Codelivery of AntimiR-21 and Gadolinium Chelate in Triple Negative Breast Cancer Theranostics.

  2026cites this paper
• Microfluidic preparation of size- and lamellarity-controlled liposomes using a static mixer and the advantage of a multilamellar structure in biosensing applications

  2026influential citation
• Pulmonary mRNA-LNP Vaccines for Rapid and Durable Protection Against Bacterial Infection

  2025cites this paper
• Automated and Parallelized Microfluidic Generation of Large and Precisely Defined Lipid Nanoparticle Libraries.

  2025cites this paper
• Peptide-Modified Lipid Nanoparticles Boost the Antitumor Efficacy of RNA Therapeutics

  2025cites this paper
• Optimizing Microfluidic Channel Design with Tilted Rectangular Baffles for Enhanced mRNA-Lipid Nanoparticle Preparation

  2025cites this paper
• Identifying fibroblast-derived sFRP2 as a therapeutic target and engineering siRNA therapy for uterine scarring

  2025cites this paper
• Investigation of structural and thermal properties of solid lipid-based nanocarriers optimized by microfluidic synthesis.

  2025cites this paper
• Landscape of small nucleic acid therapeutics: moving from the bench to the clinic as next-generation medicines

  2025cites this paper
• High-throughput, AI-assisted design and optimization of lipid nanoparticles for drug delivery.

  2025cites this paper
• Nanomedicine in Cancer Therapeutics: Current Perspectives from Bench to Bedside

  2025cites this paper
• Vaccine formulation design: challenges and opportunities

  2025cites this paper
• Targeting CCL2 silencing using polymer-lipid hybrid nanoparticles to reduce cytokine storm and inflammation in severe COVID-19.

  2025cites this paper
• Advancing Cancer Immunotherapy Using Lipid Nanoparticle-Based Approaches

  2025cites this paper
• Machine learning techniques for lipid nanoparticle formulation

  2025cites this paper
• High-throughput in vivo screening using barcoded mRNA identifies lipid nanoparticles with extrahepatic tropism for cancer immunotherapy

  2025cites this paper
• Microfluidic Production of Exosome-Mimicking Lipid Nanoparticles for Enhanced RNA Delivery: Role of Exosomal Proteins.

  2025cites this paper
• Lipid nanoparticle screening in nonhuman primates with minimal loss of life.

  2025cites this paper
• In utero lipid nanoparticle delivery achieves robust editing in hematopoietic stem cells

  2025cites this paper
• Recent Advances in Lipid Nanoparticles: Nucleic Acid Therapeutics and Targeting Strategies.

  2025cites this paper
• MicroRNA nanoformulation: a promising approach to anti-tumour activity

  2025cites this paper
• mRNA lipid nanoparticle formulation, characterization and evaluation

  2025cites this paper
• Antibody‐Nanoparticle Conjugates in Therapy: Combining the Best of Two Worlds

  2025cites this paper
• One-pot modular nanogel platform utilizing phenolic gelatin-polyethyleneimine for small RNA delivery

  2025cites this paper
• Overview on LNP-mRNA encapsulation unit operation: Mixing technologies, scalability, and influence of formulation & process parameters on physico-chemical characteristics.

  2025cites this paper
• Scalable production of uniform gene-loaded lipid nanoparticles via a fluidity-controlled membrane extrusion.

  2025cites this paper
• Microfluidic biosensors and devices for brain tumor diagnosis and monitoring.

  2025cites this paper
• Enhancing Chimeric Antigen Receptor T‐Cell Generation via Microfluidic Mechanoporation and Lipid Nanoparticles

  2025cites this paper
• Facile lipid nanoparticle size engineering approach via controllable fusion induced by depletion forces.

  2025cites this paper
• High-throughput platforms for machine learning-guided lipid nanoparticle design

  2025cites this paper
• Highly Branched Poly(β‐amino ester)s for Efficient mRNA Delivery and Nebulization Treatment of Silicosis

  2025cites this paper
• Structure and Morphology of Lipid Nanoparticles for Nucleic Acid Drug Delivery: A Review.

  2025cites this paper
• Progressing nanotechnology to improve diagnosis and targeted therapy of Diabetic Retinopathy.

  2025cites this paper
• Automated and parallelized microfluidic generation of large and precisely-defined lipid nanoparticle libraries

  2025cites this paper
• The effect of mRNA-lipid nanoparticle composition on stability during microneedle patch manufacturing.

  2025cites this paper
• Bioinspired Extracellular Vesicles for Enhanced Delivery of siRNA to Tumors.

  2025cites this paper
• Intravenous Administration of sRNA Nanoparticles for Treatment of Osteoporosis in Mice

  2025cites this paper
• Ubiquitin-specific protease 6 (USP6) mRNA lipid nanoparticles ignite anti-tumor immunity and suppress tumorigenesis in Ewing sarcoma.

  2025cites this paper
• Harnessing the Electron-Withdrawing Inductive Effect of One-Component Ionizable Amphiphilic Janus Dendrimers Unveils Cation-π Interactions and Their Important Roles to Targeted mRNA Delivery.

  2025cites this paper
• Development of a microneedle patch for delivery of mRNA-lipid nanoparticles.

  2025cites this paper
• Nanoparticles For Rescue: Innovative Therapeutic Strategy For Cardiac Repair After Myocardial Infarction

  2025cites this paper
• The Convergence of Artificial Intelligence and Microfluidics in Drug Research and Development

  2025cites this paper
• Emerging Approaches for the Discovery of Lipid-Based RNA Delivery Systems

  2025cites this paper
• Preclinical assessment of pan-influenza A virus CRISPR RNA therapeutics in a human lung alveolus chip.

  2025cites this paper
• Machine learning-assisted design of immunomodulatory lipid nanoparticles for delivery of mRNA to repolarize hyperactivated microglia

  2025cites this paper
• Theranostic Applications of Tailored Lipid-based Nanoparticles as Drug Delivery Systems for Cancer Treatment

  2025cites this paper
• Microfluidic Device Type Improves Heart mRNA Delivery In Vivo

  2025cites this paper
• Multiarm-Assisted Design of Dendron-like Degradable Ionizable Lipids Facilitates Systemic mRNA Delivery to the Spleen.

  2025cites this paper
• Activation of the coagulation cascade as a mechanism for selective nanoparticle-mediated RNA delivery to the endothelium in vivo

  2025cites this paper
• Lipid nanoparticles for engineering next generation CAR T cell immunotherapy.

  2025cites this paper
• Engineering Nanoparticles and Bioscaffolds for Targeted microRNA Delivery in Cardiovascular Regeneration—A Comprehensive Review

  2025cites this paper
• Formation of RNA lipid nanoparticles: an equilibrium process with a liquid intermediate stage.

  2025cites this paper
• Recent advances in functional lipid-based nanomedicines as drug carriers for organ-specific delivery.

  2025cites this paper
• Intelligent Design of Lipid Nanoparticles for Enhanced Gene Therapeutics.

  2025cites this paper
• Formulation screening of lyophilized mRNA-lipid nanoparticles.

  2025cites this paper
• Cationic cholesterol-dependent LNP delivery to lung stem cells, the liver, and heart

  2024influential citation
• Mesoscopic Structure of Lipid Nanoparticle Formulations for mRNA Drug Delivery: Comirnaty and Drug-Free Dispersions.

  2024cites this paper
• Simple Scattering: Lipid nanoparticle structural data repository

  2024cites this paper
• siRNA as potential therapeutic strategy for hypertension.

  2024cites this paper
• An overview on recent patents and technologies on nanoparticles for nucleic acid delivery

  2024cites this paper
• High-throughput barcoding of nanoparticles identifies cationic, degradable lipid-like materials for mRNA delivery to the lungs in female preclinical models

  2024cites this paper
• Nanoparticles Targeting Lymph Nodes for Cancer Immunotherapy: Strategies and Influencing Factors.

  2024cites this paper
• Nanotechnology-based delivery systems to overcome drug resistance in cancer

  2024cites this paper
• Antigen Presenting Cell Mimetic Lipid Nanoparticles for Rapid mRNA CAR T Cell Cancer Immunotherapy

  2024cites this paper
• Substituting Poly(ethylene glycol) Lipids with Poly(2‐ethyl‐2‐oxazoline) Lipids Improves Lipid Nanoparticle Repeat Dosing

  2024cites this paper
• Nanoparticles Based on Natural Lipids Reveal Extent of Impacts of Designed Physical Characteristics on Biological Functions.

  2024cites this paper
• Microfluidics-mediated Liposomal Nanoparticles for Cancer Therapy: Recent Developments on Advanced Devices and Technologies.

  2024cites this paper
• Expanding CAR-T cell immunotherapy horizons through microfluidics.

  2024cites this paper
• The mechanical properties of lipid nanoparticles depend on the type of biomacromolecule they are loaded with.

  2024cites this paper
• Artificial intelligence-guided design of lipid nanoparticles for pulmonary gene therapy

  2024cites this paper
• Lipid nanoparticle-mediated mRNA delivery to CD34+ cells in rhesus monkeys

  2024cites this paper
• Placenta-tropic VEGF mRNA lipid nanoparticles ameliorate murine pre-eclampsia

  2024cites this paper
• Design and development of mRNA and self-amplifying mRNA vaccine nanoformulations.

  2024cites this paper
• Navigating the nano-bio immune interface: advancements and challenges in CNS nanotherapeutics

  2024cites this paper
• Toward a Complete Elucidation of the Primary Structure-Activity in Pentaerythritol-Based One-Component Ionizable Amphiphilic Janus Dendrimers for In Vivo Delivery of Luc-mRNA.

  2024cites this paper
• Inhalable Dry Powders for Lung mRNA Delivery

  2024cites this paper
• In situ engineering of mRNA-CAR T cells using spleen-targeted ionizable lipid nanoparticles to eliminate cancer cells

  2024cites this paper
• Cryoprotectant optimization for enhanced stability and transfection efficiency of pDNA-loaded ionizable lipid nanoparticles.

  2024cites this paper
• Comprehensive analysis of lipid nanoparticle formulation and preparation for RNA delivery

  2024cites this paper
• Role of size, surface charge, and PEGylated lipids of lipid nanoparticles (LNPs) on intramuscular delivery of mRNA

  2024cites this paper
• Review of Machine Learning for Lipid Nanoparticle Formulation and Process Development.

  2024cites this paper
• Safe and effective in vivo delivery of DNA and RNA using proteolipid vehicles.

  2024cites this paper
• Extracellular vesicles versus lipid nanoparticles for the delivery of nucleic acids.

  2024cites this paper
• Elucidating lipid nanoparticle properties and structure through biophysical analyses

  2024cites this paper
• DNA aptamer-conjugated lipid nanoparticle for targeted PTEN mRNA delivery to prostate cancer cells.

  2024cites this paper
• Advancements in Nanoparticle-Based Strategies for Enhanced Antibacterial Interventions

  2024cites this paper
• Lipid nanoparticles deliver mRNA to the blood–brain barrier

  2024cites this paper
• In Vivo Delivery Processes and Development Strategies of Lipid Nanoparticles

  2024cites this paper
• Applications of microfluidics in mRNA vaccine development: A review.

  2024cites this paper
• On the Influence of Fabrication Methods and Materials for mRNA‐LNP Production: From Size and Morphology to Internal Structure and mRNA Delivery Performance In Vitro and In Vivo

  2024cites this paper
• Mechanistic Modeling of Lipid Nanoparticle Formation for the Delivery of Nucleic Acid Therapeutics

  2024cites this paper
• Synthesis of a dendritic cell-targeted self-assembled polymeric nanoparticle for selective delivery of mRNA vaccines to elicit enhanced immune responses

  2024cites this paper
• EGFR-targeted ionizable lipid nanoparticles enhance in vivo mRNA delivery to the placenta.

  2024cites this paper
• CircZFR promotes colorectal cancer progression via stabilizing BCLAF1 and regulating the miR-3127-5p/RTKN2 axis

  2024cites this paper
• Microfluidic confined acoustic streaming vortex for liposome synthesis.

  2024cites this paper
• Navigating the future: Microfluidics charting new routes in drug delivery.

  2024cites this paper
• Impact of protein corona and PEGylation on the accumulation and efficacy of lysosome-disrupting lipid nanoparticles in KRAS-mutated cancer

  2024cites this paper