Developmental regulation of expression of Ran/M1 and Ran/M2 isoforms of Ran-GTPase in mouse testis.

Two isoforms of Ran-GTPase have been described: Ran/M1 and Ran/M2. Ran/M2 is testis specific, whereas the Ran/M1 isoform is also expressed in somatic tissues. Here we show that both mRNAs, differing in 35 of the 648 nucleotides included in the ORFs, are developmentally regulated during spermatogenesis. Real-time RT-PCR experiments demonstrated that the expression of Ran/M1 and Ran/M2 increased in pachytene spermatocytes with progressive transcript accumulation until they reached the round spermatid stage, in the seminiferous epithelium of adults. In the testis, the expression of both isoforms was found to be restricted to germ cells. An expression window from early pachytene spermatocytes to late round spermatids was detected by in situ hybridization.

• Publication year

  2003

• Venue

  International Journal of Developmental Biology

• Publication date

  Unknown publication date

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1387/ijdb.12755337PMID 12755337
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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• A search for Drosophila neural precursor genes identifies ran

  2001influential reference
• Nuclear mislocalization of enzymatically active RanGAP causes segregation distortion in Drosophila.

  2001cited by this paper
• Expression of the Xenopus GTP-binding protein gene Ran during embryogenesis

  2000cited by this paper
• The Structure and Expression of the Salmo salar Ran Gene

  2000cited by this paper
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  1999cited by this paper
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  1999cited by this paper
• Ran is associated with chromosomes during starfish oocyte meiosis and embryonic mitoses

  1999cited by this paper
• Characterization of proteins that interact with the GTP-bound form of the regulatory GTPase Ran in Arabidopsis.

  1997cited by this paper
• A simple and efficient procedure for non-isotopic in situ hybridization to sectioned material.

  1994cited by this paper
• Tissue-specific expression of Ran isoforms in the mouse

  1994influential reference
• GSP1 and GSP2, genetic suppressors of the prp20-1 mutant in Saccharomyces cerevisiae: GTP-binding proteins involved in the maintenance of nuclear organization

  1993cited by this paper
• Genomic sequencing.

  1993cited by this paper
• Molecular cloning of a novel ras‐like protein from chicken

  1992cited by this paper
• Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

  1987cited by this paper
• Characterization of the multigene family encoding the mouse S16 ribosomal protein: strategy for distinguishing an expressed gene from its processed pseudogene counterparts by an analysis of total genomic DNA

  1985cited by this paper
• Genomic sequencing.

  1984cited by this paper
• Spermatogenic cells of the prepuberal mouse: isolation and morphological characterization

  1977influential reference
• Duration of spermatogenesis in the mouse and timing of stages of the cycle of the seminiferous epithelium.

  1956cited by this paper
• A description of spermiogenesis in the mouse and its use in analysis of the cycle of the seminiferous epithelium and germ cell renewal.

  1956cited by this paper

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  2012cites this paper
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  2004cites this paper
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