Current Trends in Protein Purification : A Review

The present review is based on papers published between 1990 and 2020 and gives Comparative information about the most common protein purification techniques Gel-Filtration, Chromatography, Ion-Exchange Chromatography, Electrophoresis, Affinity Chromatography, and Dialysis, High-Pressure Liquid Chromatography. and their applications.

• Publication year

  2020

• Venue

  International Journal of Scientific Research in Science and Technology

• Publication date

  2020-12-03

• Fields of study

  Materials Science, Chemistry

• Identifiers
  DOI 10.32628/ijsrst207645
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Purification of low‐abundance lysozyme in egg white via free‐flow electrophoresis with gel‐filtration chromatography

  2020cited by this paper
• A simple purification procedure of buffalo lung cathepsin H, its properties and influence of buffer constituents on the enzyme activity

  2020cited by this paper
• Polystyrene adsorbents: rapid and efficient surrogate for dialysis in membrane protein purification

  2020cited by this paper
• Excess acetone extraction in silk protein solution greatly accelerates the regeneration progress of silk fibroin for desalting and purification.

  2020cited by this paper
• Affinity purification of Car9-tagged proteins on silica-derivatized spin columns and 96-well plates.

  2020cited by this paper
• Affinity Membranes and Monoliths for Protein Purification

  2019cited by this paper
• When to Avoid Dialysis during Protein Purification?

  2019cited by this paper
• Purification and characterization of alkaline protease with novel properties from Bacillus cereus strain S8

  2018cited by this paper
• An efficient methodology for the purification of date palm peroxidase: Stability comparison with horseradish peroxidase (HRP)

  2018cited by this paper
• Modelling the effect of temperature on the gel-filtration chromatographic protein separation

  2018cited by this paper
• Dual column approach for the purification of zinc finger proteins by immobilized metal affinity chromatography

  2018cited by this paper
• Extraction, purification, and activity of protease from the leaves of Moringa oleifera

  2018cited by this paper
• Effects of bed compression on protein separation on gel filtration chromatography at bench and pilot scale

  2017cited by this paper
• Comparison of antimicrobial peptide purification via free‐flow electrophoresis and gel filtration chromatography

  2017cited by this paper
• Differential effects of DEAE negative mode chromatography and gel-filtration chromatography on the charge status of Helicobacter pylori neutrophil-activating protein

  2017cited by this paper
• Lactoperoxidase purification from whey by using dye affinity chromatography

  2017cited by this paper
• Refined purification strategy for reliable proteomic profiling of HDL2/3: Impact on proteomic complexity

  2016cited by this paper
• Purification and Characterization of an Antioxidant Protein from Fertilized Eggs

  2016cited by this paper
• Two-step ion-exchange chromatographic purification combined with reversed-phase chromatography to isolate C-peptide for mass spectrometric analysis.

  2016cited by this paper
• Purification, Characterization, and Sensitivity to Pesticides of Carboxylesterase From Dendrolimus superans (Lepidoptera: Lasiocampidae)

  2014cited by this paper
• Induction of Apoptotic Effects of Antiproliferative Protein from the Seeds of Borreria hispida on Lung Cancer (A549) and Cervical Cancer (HeLa) Cell Lines

  2014cited by this paper
• Gel filtration chromatography (size exclusion chromatography) of proteins.

  2014cited by this paper
• Protein Purification by Affinity Chromatography

  2012cited by this paper
• Isolation, Purification, and Characterization of Xylanase Produced by a New Species of Bacillus in Solid State Fermentation

  2012cited by this paper
• Purification and characterization of an alkaline protease from Bacillus licheniformis UV-9 for detergent formulations

  2012cited by this paper
• Simultaneous extraction and purification of aloe polysaccharides and proteins using ionic liquid based aqueous two-phase system coupled with dialysis membrane

  2012cited by this paper
• Purification of Tropomyosin, Paramyosin, Actin, Tubulin, Troponin and Kinases for Chemiproteomics and Its Application to Different Scientific Fields

  2011cited by this paper
• Separation of biological proteins by liquid chromatography.

  2010cited by this paper
• A versatile multidimensional protein purification system with full internet remote control based on a standard HPLC system.

  2009cited by this paper
• Purification and characterization of a novel antifungal protein from Bacillus subtilis strain B29

  2009cited by this paper
• Purification of prenylated proteins by affinity chromatography on cyclodextrin-modified agarose.

  2009cited by this paper
• High-throughput protein purification using an automated set-up for high-yield affinity chromatography.

  2006cited by this paper
• HPLC techniques for proteomics analysis--a short overview of latest developments.

  2006cited by this paper
• One-step purification of a recombinant protein from a whole cell extract by reversed-phase high-performance liquid chromatography.

  2006cited by this paper
• Protein purification by affinity precipitation.

  2003cited by this paper
• An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography

  2003cited by this paper
• Purification and Identification of a 42-Kilodalton Abscisic Acid-Specific-Binding Protein from Epidermis of Broad Bean Leaves1

  2002cited by this paper
• Comparison of three directly coupled HPLC MS/MS strategies for identification of proteins from complex mixtures: single-dimension LC-MS/MS, 2-phase MudPIT, and 3-phase MudPIT

  2002cited by this paper
• Automated identification of amino acid sequence variations in proteins by HPLC/microspray tandem mass spectrometry.

  2000cited by this paper
• Chelated mercury as a ligand in immobilized metal ion affinity chromatography of proteins.

  2000cited by this paper
• An overview of affinity chromatography.

  2000cited by this paper

• Biodegradation of synthetic dyes using Aspergillus fumigatus-derived laccase immobilized in alginate matrices

  2025cites this paper
• Characteristics of hydroxyapatite/lignocellulose composites as stationary phase for the purification applications of phycobiliprotein from red algae

  2025cites this paper
• Optimized isolation and purification of native glycoprotein B from herpes simplex virus 1: a streamlined approach

  2025cites this paper
• Recovery of Proteins and Bioactive Peptides From Potato Peels

  2025cites this paper
• Nanozyme activity of silver nanowires for colorimetric detection of bisphenol A following salting-out assisted liquid-liquid extraction

  2025cites this paper
• Separation methods for food protein purification and analysis

  2024cites this paper
• Microbial proteases: A next generation green catalyst for industrial, environmental and biomedical sustainability

  2023cites this paper
• Gel Protein Extraction’s Impact on Conformational Epitopes of Linear Non-Tagged MPT64 Protein

  2023cites this paper
• Hydrolyzed Proteins and Vegetable Peptides: Anti-Inflammatory Mechanisms in Obesity and Potential Therapeutic Targets

  2022cites this paper
• Aptamer loaded superparamagnetic beads for selective capturing and gentle release of activated protein C

  2022cites this paper