Isolation, Purification, and Characterization of Xylanase Produced by a New Species of Bacillus in Solid State Fermentation

A thermoalkalophilic new species of Bacillus, similar to Bacillus arseniciselenatis DSM 15340, produced extracellular xylanase under solid state fermentation when wheat bran is used as carbon source. The extracellular xylanase was isolated by ammonium sulfate (80%) precipitation and purified using ion exchange chromatography. The molecular weight of xylanase was ~29.8 kDa. The optimum temperature and pH for the enzyme activity were 50°C and pH 8.0. The enzyme was active on birchwood xylan and little active on p-nitrophenyl xylopyranoside but not on Avicel, CMC, cellobiose, and starch, showing its absolute substrate specificity. For birchwood xylan, the enzyme gave a Km 5.26 mg/mL and Vmax 277.7 μmol/min/mg, respectively. In addition, the xylanase was also capable of producing high-quality xylo-oligosaccharides, which indicated its application potential not only in pulp biobleaching processes but also in the nutraceutical industry.

• Publication year

  2012

• Venue

  International Journal of Microbiology

• Publication date

  2012-01-17

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1155/2012/683193PMID 22315613PMCID 3270423
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Isolation

  2024cited by this paper
• I and J

  2012influential reference
• Boron-based pronucleophiles in catalytic (asymmetric) C(sp3)–allyl cross-couplings

  2012cited by this paper
• Production, purification and characterisation of a novel halostable xylanase from Bacillus sp. NTU‐06

  2010cited by this paper
• Modulation of natural killer cell activity by viruses.

  2010cited by this paper
• Production, purification and characterization of celullase-free xylanase from Aspergillus terreus UL 4209

  2008cited by this paper
• Purification and characterization of extracellular xylanase from Streptomyces cyaneus SN32.

  2008cited by this paper
• Production and partial characterization of cellulase free xylanase by Bacillus subtilis C 01 using agriresidues and its application in biobleaching of nonwoody plant pulps

  2007cited by this paper
• Molecular cloning and heterologous expression of a new xylanase gene from Plectosphaerella cucumerina

  2007cited by this paper
• Isolation, purification and characterization of the xylanase produced by Arthrobacter sp. MTCC 5214 when grown in solid-state fermentation

  2006cited by this paper
• Purification and properties of a xylanase produced by Bacillus circulans BL53 on solid-state cultivation

  2006cited by this paper
• Influence of cultivation temperature on the ligninolytic activity of selected fungal strains

  2006influential reference
• Purification of xylanase from alkaliphilic Bacillus sp. K-8 by using corn husk column

  2006cited by this paper
• Bacillus arsenicus sp. nov., an arsenic-resistant bacterium isolated from a siderite concretion in West Bengal, India.

  2005cited by this paper
• Carbohydrate Metabolism in Citric Acid Fermentation

  2005cited by this paper
• Purification and characterization of a thermoalkalophilic xylanase from Bacillus sp.

  2005cited by this paper
• Development of a plate technique for screening of polysaccharide-degrading microorganisms by using a mixture of insoluble chromogenic substrates.

  2004cited by this paper
• Xylanase production by Ganoderma lucidum on liquid and solid state fermentation.

  2003influential reference
• Three-dimensional structures of thermophilic beta-1,4-xylanases from Chaetomium thermophilum and Nonomuraea flexuosa. Comparison of twelve xylanases in relation to their thermal stability.

  2003cited by this paper
• Microbial hemicellulases.

  2003influential reference
• Interlaboratory testing of methods for assay of xylanase activity

  2002cited by this paper
• Purification and characterization of two cellulase free xylanases from an alkaliphilic Bacillus firmus

  2002cited by this paper
• Microbial xylanases and their industrial applications: a review

  2001cited by this paper
• MICROBIAL XYLANASES FOR PAPER INDUSTRY

  1999cited by this paper
• Molecular and biotechnological aspects of xylanases.

  1999cited by this paper
• Production of Aspergillus xylanase by lignocellulosic waste fermentation and its application

  1999cited by this paper
• Purification and characterization of an alkaline xylanase from alkaliphilic Micrococcus sp AR-135

  1998cited by this paper
• Production of xylanases from a newly isolated alkalophilic thermophilic Bacillus sp.

  1998cited by this paper
• Xylanase production by thermophilic Bacillus licheniformis A99 in solid-state fermentation

  1997cited by this paper
• β‐Xylosidase and xylanase characterization and production by Streptomyces sp. CH‐M‐1035

  1997cited by this paper
• Purification and Characterization of Two Xylanases from Alkalophilic Cephalosporium sp. Strain RYM-202

  1996cited by this paper
• Production of fungal xylanases

  1996cited by this paper
• Purification and characterization of a thermophilic alkaline xylanase from thermoalkaliphilic Bacillus sp. strain TAR-1

  1995cited by this paper
• Purification and properties of xylanase A from alkali-tolerant Bacillus sp. strain BP-23

  1995cited by this paper
• Purification, Characterization, and Substrate Specificities of Multiple Xylanases from Streptomyces sp. Strain B-12-2

  1994cited by this paper
• Purification and Characterization of Aeromonas caviae ME-1 Xylanase V, Which Produces Exclusively Xylobiose from Xylan

  1994cited by this paper
• Purification and some properties of an alkaline xylanase from alkaliphilic Bacillus sp. strain 41M-1

  1993cited by this paper
• Xylanase active at high pH from an alkalotolerant Cephalosporium species

  1993cited by this paper
• Low-molecular-weight xylanase from Trichoderma viride

  1991cited by this paper
• Purification and properties of thermostable xylanase and beta-xylosidase produced by a newly isolated Bacillus stearothermophilus strain

  1990cited by this paper
• CHAPTER 13 – CHARACTERIZATION OF PULPS TREATED WITH HEMICELLULOLYTIC ENZYMES PRIOR TO BLEACHING

  1990cited by this paper
• 1,4-β-d-Xylan xylohydrolase of Sclerotium rolfsii

  1988cited by this paper
• Measurement of cellulase activities

  1987cited by this paper
• Purification and characterization of a thermostable xylanase from a thermophilic fungus Thermoascus aurantiacus

  1987cited by this paper
• The neighbor-joining method: a new method for reconstructing phylogenetic trees.

  1987cited by this paper
• Endospore-forming gram-positive rods and cocci,

  1986cited by this paper
• Two types of xylanases of alkalophilic Bacillus sp. No. C-125

  1985cited by this paper
• Purification of Three Types of Xylanases from an Alkalophilic Aeromonas sp.

  1985cited by this paper
• Purification and properties of two endo-1,4-beta-xylanases from Irpex lacteus (Polyporus tulipiferae).

  1985cited by this paper
• Purification and properties of endoxylanase produced by Bacillus pumilus.

  1983cited by this paper
• Isolation and Characterization of a Xylanase from Bacillus subtilis

  1983cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper
• Methods in Enzymology , Vol

  1966cited by this paper
• Agricultural and biological chemistry

  1961cited by this paper
• Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar

  1959cited by this paper
• Carbohydrate metabolism in citric acid fermentation. 4. Purification and properties of aldolase from Aspergillus niger.

  1956cited by this paper
• Protein measurement with the Folin phenol reagent.

  1951cited by this paper
• Detection of Sugars on Paper Chromatograms

  1950cited by this paper
• The Determination of Enzyme Dissociation Constants

  1934cited by this paper
• Production

  1929cited by this paper

• Review of Xylanases: Sources, Engineering and Biotechnological Use

  2026cites this paper
• Enrichment of fruit juice clarification from xylanase immobilized onto polystyrene surface using cysteine-capped copper nanoparticles.

  2026cites this paper
• Vasorelaxation and antihypertensive activity of standardized extract of Gymnema sylvestre in rodent model.

  2026cites this paper
• Mapping the genomic position of xylanase genes on Bacillus safensis FB03 and optimizing the xylanase fermentation medium by Box-Behnken Design from an unconventional carbon source

  2025cites this paper
• Efficient Assessment and Optimisation of Medium Components Influencing Extracellular Xylanase Production by Pediococcus pentosaceus G4 Using Statistical Approaches

  2025cites this paper
• Process optimization and partial purification of a highly catalytic xylanase from high-altitude novel Bacillus altitudinis strain for efficient production of functional prebiotic xylooligosaccharides.

  2025cites this paper
• Unlocking the Power of Xylooligosaccharides as Nutraceuticals: Sources, Production Strategies, and Applications

  2025cites this paper
• Production of xylo-oligosaccharides from corncob using high efficiency xylanase from Trichoderma harzianum 4FR8

  2025cites this paper
• Process optimization studies on xylanase production and bioethanol fermentation

  2025cites this paper
• Xylanase of Bacillus sp. NIORKP76 grown under solid state fermentation: production, purification, characterization and its saccharification potential

  2025influential citation
• Valorization of Goruba (Hyphaene thebaica L) Fruit Waste for Optimized Prebiotic Xylooligosaccharides Production via Aspergillus flavus Xylanase

  2025influential citation
• Real-time pH and temperature monitoring in solid-state fermentation reveals culture physiology and optimizes enzyme harvesting for tailored applications

  2025influential citation
• Expression and Characterization of Recombinant Endo-β-1,4-D-xylanases XynBTN63D from Soil Termite Abdomen in Escherichia coli BL21 (DE3)

  2024cites this paper
• The utilization of Trichoderma viride in Optimising Xylanase Production from Coffee Cherry Processing Waste

  2024cites this paper
• Genetic Diversity and Functional Potential of Streptomyces spp. Isolated from Pachmarhi Biosphere Reserve, India

  2024cites this paper
• A review on xylanase sources, classification, mode of action, fermentation processes, and applications as a promising biocatalyst

  2024cites this paper
• Xylanase from Neobacillus sedimentimangrovi UE25: Characterization, purification and applications

  2024cites this paper
• Temperature dependence of acids recovery from wastewater electro-fermentation

  2024cites this paper
• Coffee cherry processing waste to produce value-added products through clean production biorefineries

  2023cites this paper
• In silico studies of fungal xylanase enzymes: structural and functional insight towards efficient biodegradation of lignocellulosic biomass

  2023cites this paper
• Bacillus genus industrial applications and innovation: First steps towards a circular bioeconomy.

  2023cites this paper
• Sustainable and Eco-Friendly Production of Xylanase and Other Enzymes from Tomato Paste through Solid-State Fermentation Using Bacillus safensis MABS6

  2023influential citation
• Optimization of xylanase production by Pichia kudriavzevii and Candida tropicalis isolated from the wood product workshop

  2023cites this paper
• Aqueous two-phase extraction and characterization of thermotolerant alkaliphilic Cladophora hutchinsiae xylanase: biochemical properties and potential applications in fruit juice clarification and fish feed supplementation

  2023cites this paper
• Purification and Characterization of Xylanase Produced by Aspergillus fumigatus Isolated from the Northern Border Region of Saudi Arabia

  2023cites this paper
• Proficient Lignocellulolytic Novel Bacterial Isolates from Diversified Galiyat Forests of Lower Himalaya

  2023cites this paper
• Dissolved oxygen on xylanase production by Trichoderma reesei using Reutalis trisperma press cake as an additional substrate

  2023influential citation
• An insight into microbial sources, classification, and industrial applications of xylanases: A rapid review

  2023cites this paper
• Distribution and diversity in microbial enzymes

  2022cites this paper
• Microbial Xylanases: A Review

  2022cites this paper
• For biotechnological applications: Purification and characterization of recombinant and nanoconjugated xylanase enzyme from thermophilic Bacillus subtilis

  2022cites this paper
• Enzymatic synthesis of silver nanoparticles: Mechanisms and applications

  2022cites this paper
• A systematic review on potential microbial carbohydrases: current and future perspectives

  2022cites this paper
• Three-Step Purification and Characterization of Organic Solvent-Tolerant and Alkali-Thermo-Tolerant Xylanase from Bacillus paramycoides T4 [MN370035]

  2022cites this paper
• Cereal-Based Solid-State Fermentation and Anti-Inflammatory Potential of Paecilomyces cicadae SH1 (Ascomycota) Isolated in China.

  2022cites this paper
• Downstream Process of Xylanase Production from Oil Palm Empty Fruit Bunches: A Review

  2022cites this paper
• Rahnella sp., a Dominant Symbiont of the Core Gut Bacteriome of Dendroctonus Species, Has Metabolic Capacity to Degrade Xylan by Bifunctional Xylanase-Ferulic Acid Esterase

  2022cites this paper
• Utilization of Agroresidues for the Production of Xylanase by Bacillus safensis XPS7 and Optimization of Production Parameters

  2022cites this paper
• Synthesis of two novel bio-based hydrogels using sodium alginate and chitosan and their proficiency in physical immobilization of enzymes

  2022cites this paper
• Protein Purification by Ion Exchange Chromatography

  2022cites this paper
• Expression, Characterization and Its Deinking Potential of a Thermostable Xylanase From Planomicrobium glaciei CHR43

  2021cites this paper
• Purification and characterization of two thermostable xylanases from a halotolerant Bacillus sp. Asc6BA isolated from Salar de Ascotán, Atacama Desert

  2021cites this paper
• Creating wealth from waste: An approach for converting organic waste in to value-added products using microbial consortia

  2021cites this paper
• Primary capture of Bacillus subtilis xylanase from crude feedstock using alcohol/salt liquid biphasic flotation

  2021cites this paper
• Eco-friendly degumming of natural fibers for textile applications: A comprehensive review

  2021cites this paper
• The Delignification of Plants Residual for Saccharification Growth by the Fungal Consortium: A Practical Approach

  2021influential citation
• Extremophilic Prokaryotic Endoxylanases: Diversity, Applicability, and Molecular Insights

  2021cites this paper
• Whole-Genome Sequence Data Analysis of Anoxybacillus kamchatkensis NASTPD13 Isolated from Hot Spring of Myagdi, Nepal

  2021cites this paper
• Chimeric bi-functional enzyme possessing xylanase and deacetylase activity for hydrolysis of agro-biomass rich in acetylated xylan.

  2021cites this paper
• Bioconversion Technologies: Hydrolytic Enzyme Treatment of Food Waste

  2021cites this paper
• Purification and characterization of two thermostable xylanases from a halotolerant Bacillus sp. Asc6BA isolated from Salar de Ascotán, Atacama Desert

  2021cites this paper
• BIOETHANOL PRODUCTION FROM APPLE POMACE USING CO-CULTURES WITH SACCHAROMYCES CEREVISIAE IN SOLID-STATE FERMENTATION

  2020cites this paper
• Study of the intracellular xylanolytic activity of the phytopathogenic fungus Sporisorium reilianum

  2020cites this paper
• A novel thermostable cellulase cocktail enhances lignocellulosic bioconversion and biorefining in a broad range of pH.

  2020cites this paper
• Combinatorial Biobleaching of Mixedwood Pulp with Lignolytic and Hemicellulolytic Enzymes for Paper Making

  2020cites this paper
• Optimization of Xylanase Activity in the Presence of Salts of Different Metals for the Pulping of Indigenous Non-Woody Raw Materials (Gossypium Arborium)

  2020cites this paper
• Factorial experimental design for xylanase production by Bacillus sp. isolated from Malaysia landfill soil

  2020cites this paper
• Fermentation of simple and complex substrates to biohydrogen using pure Bacillus cereus RTUA and RTUB strains

  2020cites this paper
• Xylanases: For digestion of hemicellulose

  2020cites this paper
• Effects of Enzymes on the Refining of Different Pulps.

  2020cites this paper
• Pilot scale production of recombinant hemicellulases and their saccharification potential

  2020cites this paper
• Improvement of PersiXyn2 activity and stability in presence of Trehalose and proline as a natural osmolyte.

  2020cites this paper
• Lignolytic and hemicellulolytic enzyme cocktail production from Bacillus tequilensis LXM 55 and its application in pulp biobleaching

  2020cites this paper
• Process optimization for simultaneous production of cellulase, xylanase and ligninase by Saccharomyces cerevisiae SCPW 17 under solid state fermentation using Box-Behnken experimental design

  2020cites this paper
• Isolation and identification of xylanase producing thermophilic bacteria from compost piles and optimization of xylanase production

  2020influential citation
• An efficient nano-biocatalyst for lignocellulosic biomass hydrolysis: Xylanase immobilization on organically modified biogenic mesoporous silica nanoparticles.

  2020cites this paper
• Molecular characterization of thermostable hydrolytic Enzymes producing bacteria isolated from hot spring of Ras Sedr, South Sinai, Egypt.

  2020cites this paper
• Optimization of xylanase production by Bacillus tequilensis strain UD-3 using economical agricultural substrate and its application in rice straw pulp bleaching

  2020cites this paper
• Current Trends in Protein Purification : A Review

  2020cites this paper
• A novel high performance metagenome-derived alkali-thermostable endo-β-1,4-glucanase for lignocellulosic biomass hydrolysis in the harsh conditions

  2020cites this paper
• Ultrasonic assisted fermentation for production of xylanase enzyme using locally isolated strain of Bacilus badius

  2020cites this paper
• ASSESSMENT OF XYLANOLYTIC AND CELLULOLYTIC ACTIVITIES OF ANAEROBIC BACTERIAL COMMUNITY IN THE RUMEN OF CAMEL USING DIFFERENT LIGNOCELLULOSIC SUBSTRATES

  2019cites this paper
• Various growth attributes of Escherichia coli cultures supplemented with Aloe vera as substrate

  2019cites this paper
• Cellulase and xylanase synergism in industrial biotechnology

  2019cites this paper
• Xylan Production from Corn Cobs for Isolation of Xylanase-Producing Bacteria

  2019cites this paper
• A detailed overview of xylanases: an emerging biomolecule for current and future prospective

  2019cites this paper
• Diversity in Xylan-degrading Prokaryotes and Xylanolytic Enzymes and Their Bioprospects

  2019cites this paper
• Xylanases from marine microorganisms: A brief overview on scope, sources, features and potential applications.

  2019cites this paper
• Application of carboxymethyl cellulose-g-poly(acrylic acid-co-acrylamide) hydrogel sponges for improvement of efficiency, reusability and thermal stability of a recombinant xylanase

  2019cites this paper
• Cloning, Purification, and Characterization of Recombinant Thermostable β-Xylanase Tnap_0700 from Thermotoga naphthophila

  2019cites this paper
• Screening of Culture Conditions for Production of Xylanase from Landfill Soil Bacteria

  2019influential citation
• Production, characteristics, and biotechnological applications of microbial xylanases

  2019cites this paper
• Cellulolytic and xylanolytic enzymes of thermophiles for the production of renewable biofuels

  2019cites this paper
• Purification and characterization of a thermo-acid/alkali stable xylanases from Aspergillus oryzae LC1 and its application in Xylo-oligosaccharides production from lignocellulosic agricultural wastes.

  2019cites this paper
• Identification and characterization of a novel thermostable xylanase from camel rumen metagenome.

  2019cites this paper
• A novel endo-β-1,4-xylanase from Pediococcus acidilactici GC25; purification, characterization and application in clarification of fruit juices.

  2019cites this paper
• Mining of camel rumen metagenome to identify novel alkali-thermostable xylanase capable of enhancing the recalcitrant lignocellulosic biomass conversion.

  2019cites this paper
• Xylanase immobilization on magnetite and magnetite core/shell nanocomposites using two different flexible alkyl length organophosphonates: Linker length and shell effect on enzyme catalytic activity.

  2018cites this paper
• Morphological, enzymatic screening, and phylogenetic analysis of thermophilic bacilli isolated from five hot springs of Myagdi, Nepal

  2018cites this paper
• Screening of a Soil Bacteria Collection for the Production of Alkali Thermostable Xylanases

  2018cites this paper
• In silico studies on bacterial xylanase enzyme: Structural and functional insight

  2018cites this paper
• Utility of acidic xylanase of Bacillus subtilis subsp. subtilis JJBS250 in improving the nutritional value of poultry feed

  2018cites this paper
• Isolation, Screening, Identification, and Optimization of Xylanase Producing Bacteria from Forests of Western Ghats, India

  2018cites this paper
• Identification and characterization of GH11 xylanase and GH43 xylosidase from the chytridiomycetous fungus, Rhizophlyctis rosea

  2018cites this paper
• Isolation, Identification and Partial Optimization of Novel Xylanolytic Bacterial Isolates from Bhilai-Durg Region, Chhattisgarh, India

  2018cites this paper
• Production, Purification, and Characterization of Thermostable Alkaline Xylanase From Anoxybacillus kamchatkensis NASTPD13

  2018influential citation
• Production of a thermostable and alkali resistant endoxylanase by Bacillus subtilis DFR40 and its application for preparation of prebiotic xylooligosaccharides

  2018cites this paper
• Biotechnology of Microbial Xylanase: Overview

  2018cites this paper
• Thermodynamics characterization and potential textile applications of Trichoderma longibrachiatum KT693225 xylanase

  2018cites this paper
• Recent Advances and Industrial Applications of Microbial Xylanases: A Review

  2018cites this paper