Overexpression of Krueppel like factor 3 promotes subcutaneous adipocytes differentiation in goat Capra hircus.

Previous research reported that KLF3 plays different roles in the regulation of adipose deposition across species. However, the exact function of KLF3 in goat subcutaneous adipocyte remains unknown. Here, the goat KLF3 gene was firstly cloned and showed that the mRNA sequence of the goat KLF3 gene was 1,264 bp (GenBank accession number: KU041753.1) and its coding sequence was 1,037 bp, encoding 345 amino acids with three classic zinc finger domains of KLFs family at its C-terminus. The alignment of the amino acid sequence of KLF3 among various species demonstrated that goat had the highest homology to that of sheep, presenting 99.4% similarity, while the homology similarity to that of mice presented only 93.62% in contrast. Furthermore, KLF3 had highest mRNA level in fat tissue and lowest level in the heart in comparison. Additionally, the mRNA level of KLF3 gradually tended to increase during adipogenesis. Interestingly, overexpression of KLF3 increased lipid accumulation. In line with this, the gain-of-function of KLF3 dramatically elevated the mRNA levels of TG synthetic genes and adipogenic maker genes (p < .01) . Moreover, overexpression of KLF3 upregulated all the potential target genes, except for C/EBPα. These results suggested that KLF3 is a positive regulator for subcutaneous adipocyte differentiation in goats.

• Publication year

  2021

• Venue

  Animal science journal = Nihon chikusan Gakkaiho

• Publication date

  2021-01-01

• Fields of study

  Biology, Agricultural and Food Sciences, Medicine

• Identifiers
  DOI 10.1111/asj.13514PMID 33522088
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Bta‐miR‐149‐5p inhibits proliferation and differentiation of bovine adipocytes through targeting CRTCs at both transcriptional and posttranscriptional levels

  2020cited by this paper
• Effect of let-7a overexpression on the differentiation of conjunctiva mesenchymal stem cells into photoreceptor-like cells

  2019cited by this paper
• Molecular Carcinogenesis

  2019cited by this paper
• Advances in Sheep and Goat Meat Products Research.

  2019cited by this paper
• Fibroblast growth factor 21 regulates lipid accumulation and adipogenesis in goat intramuscular adipocyte

  2019cited by this paper
• Knockdown of LXRα Inhibits Goat Intramuscular Preadipocyte Differentiation

  2018cited by this paper
• A novel brown adipocyte-enriched long non-coding RNA that is required for brown adipocyte differentiation and sufficient to drive thermogenic gene program in white adipocytes.

  2018cited by this paper
• Krüppel-like factor 10 (KLF10) is transactivated by the transcription factor C/EBPβ and involved in early 3T3-L1 preadipocyte differentiation

  2018cited by this paper
• Transcriptional Regulation of Adipogenesis.

  2017cited by this paper
• THE QUALITY OF GOAT MEAT AND IT'S IMPACT ON HUMAN HEALTH

  2016cited by this paper
• Relationships between Rodent White Adipose Fat Pads and Human White Adipose Fat Depots

  2016cited by this paper
• Role of Phosphotyrosine Interaction Domain Containing 1 in Porcine Intramuscular Preadipocyte Proliferation and Differentiation

  2016cited by this paper
• KLF13 promotes porcine adipocyte differentiation through PPARγ activation

  2015cited by this paper
• Phosphorylation of Krüppel-like Factor 3 (KLF3/BKLF) and C-terminal Binding Protein 2 (CtBP2) by Homeodomain-interacting Protein Kinase 2 (HIPK2) Modulates KLF3 DNA Binding and Activity*

  2015cited by this paper
• Expression and functional analyses of Krüppel-like factor 3 in chicken adipose tissue

  2014cited by this paper
• Oleate promotes differentiation of chicken primary preadipocytes in vitro

  2014cited by this paper
• Recent developments in altering the fatty acid composition of ruminant-derived foods.

  2013cited by this paper
• Loss of Krüppel-Like Factor 3 (KLF3/BKLF) Leads to Upregulation of the Insulin-Sensitizing Factor Adipolin (FAM132A/CTRP12/C1qdc2)

  2013cited by this paper
• Meat Science and Muscle Biology Symposium: manipulating mesenchymal progenitor cell differentiation to optimize performance and carcass value of beef cattle.

  2013cited by this paper
• Regulation of lipoprotein assembly, secretion and fatty acid β-oxidation by Krüppel-like transcription factor, klf-3.

  2013cited by this paper
• Adipogenesis: from stem cell to adipocyte.

  2012cited by this paper
• Partner in fat metabolism: role of KLFs in fat burning and reproductive behavior

  2011cited by this paper
• A functional family-wide screening of SP/KLF proteins identifies a subset of suppressors of KRAS-mediated cell growth.

  2011cited by this paper
• DGAT enzymes are required for triacylglycerol synthesis and lipid droplets in adipocytes[S]

  2011cited by this paper
• The mammalian zinc finger transcription factor Krüppel‐like factor 3 (KLF3/BKLF)

  2011cited by this paper
• Regulation of fat storage and reproduction by Krüppel-like transcription factor KLF3 and fat-associated genes in Caenorhabditis elegans.

  2011cited by this paper
• Programming of marginal zone B-cell fate by basic Kruppel-like factor (BKLF/KLF3).

  2011cited by this paper
• KLF3 Regulates Muscle-Specific Gene Expression and Synergizes with Serum Response Factor on KLF Binding Sites

  2010cited by this paper
• Effects of early gestational undernutrition on fetal growth, organ development, and placentomal composition in the bovine.

  2009cited by this paper
• Krüppel-like family of transcription factors: an emerging new frontier in fat biology

  2009cited by this paper
• Targeted Disruption of the Basic Krüppel-Like Factor Gene (Klf3) Reveals a Role in Adipogenesis

  2008cited by this paper
• Lipid droplets: a classic organelle with new outfits

  2008cited by this paper
• A Network of Krüppel-like Factors (Klfs)

  2008cited by this paper
• Thematic review series: Adipocyte Biology. The perilipin family of structural lipid droplet proteins: stabilization of lipid droplets and control of lipolysis Published, JLR Papers in Press, September 18, 2007.

  2007cited by this paper
• Erythroid Krüppel-Like Factor Directly Activates the Basic Krüppel-Like Factor Gene in Erythroid Cells

  2007cited by this paper
• Overexpression of Kruppel-like factor 7 regulates adipocytokine gene expressions in human adipocytes and inhibits glucose-induced insulin secretion in pancreatic beta-cell line.

  2006cited by this paper
• Krüppel-like Factor-6 Promotes Preadipocyte Differentiation through Histone Deacetylase 3-dependent Repression of DLK1*

  2005cited by this paper
• Transcription Suppression of Peroxisome Proliferator-Activated Receptor γ2 Gene Expression by Tumor Necrosis Factor α via an Inhibition of CCAAT/ Enhancer-binding Protein δ during the Early Stage of Adipocyte Differentiation

  2004cited by this paper
• Enzymes of triacylglycerol synthesis and their regulation.

  2004cited by this paper
• Transcription suppression of peroxisome proliferator-activated receptor gamma2 gene expression by tumor necrosis factor alpha via an inhibition of CCAAT/ enhancer-binding protein delta during the early stage of adipocyte differentiation.

  2004cited by this paper
• Properties and physiological significance of fatty acid binding proteins

  2003cited by this paper
• Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

  2001cited by this paper
• Krüppel-like Factors: Three Fingers in Many Pies*

  2001cited by this paper
• The roles of PPARs in adipocyte differentiation.

  2001cited by this paper
• Transcriptional regulation of adipogenesis.

  2000cited by this paper

• Effects of the FHL2 gene on the development of subcutaneous and intramuscular adipocytes in goats

  2024cites this paper
• The Characterization of Subcutaneous Adipose Tissue in Sunit Sheep at Different Growth Stages: A Comprehensive Analysis of the Morphology, Fatty Acid Profile, and Metabolite Profile

  2024cites this paper
• Prognostic value of KLFs family genes in renal clear cell carcinoma

  2024cites this paper
• Pan-cancer analysis of Krüppel-like factor 3 and its carcinogenesis in pancreatic cancer

  2023cites this paper
• miR-130b duplex (miR-130b-3p/miR-130b-5p) negatively regulates goat intramuscular preadipocyte lipid droplets accumulation by inhibiting Krüppel-like factor 3 expression

  2023cites this paper
• The Kruppel-like Factor (KLF) Family, Diseases, and Physiological Events.

  2023cites this paper
• Overexpression of goat STEAP4 promotes the differentiation of subcutaneous adipocytes.

  2022cites this paper
• Krüppel-like factors family regulation of adipogenic markers genes in bovine cattle adipogenesis.

  2022cites this paper
• RNA-seq analysis reveals the positive role of KLF5 in the differentiation of subcutaneous adipocyte in goats.

  2021cites this paper