Next generation high density self assembling functional protein arrays

We developed a high-density self-assembling protein microarray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of proteins that are produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000 unique human cDNAs and obtained high yields of protein expression and capture with minimal variation and good reproducibility. This method will enable various experimental approaches to study protein function in high throughput.

• Publication year

  2008

• Venue

  Nature Methods

• Publication date

  2008-05-11

• Fields of study

  Biology, Medicine, Materials Science, Engineering

• Identifiers
  DOI 10.1038/nmeth.1210PMID 18469824PMCID 3070491
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Cell-Free Protein Expression

  2011cited by this paper
• Printing protein arrays from DNA arrays

  2008cited by this paper
• A Biomedically Enriched Collection of 7000 Human ORF Clones

  2008cited by this paper
• Nature Methods

  2007cited by this paper
• A Full-Genomic Sequence-Verified Protein-Coding Gene Collection for Francisella tularensis

  2007cited by this paper
• Generation of High Density Protein Microarrays by Cell-free in Situ Expression of Unpurified PCR Products *

  2006cited by this paper
• Protein chip fabrication by capture of nascent polypeptides

  2006cited by this paper
• IL-13受体α2降低血吸虫病肉芽肿的炎症反应并延长宿主存活时间[英]/Mentink-Kane MM,Cheever AW，Thompson RW，et al//Proc Natl Acad Sci U S A

  2005cited by this paper
• Protein microarrays as tools for functional proteomics.

  2005cited by this paper
• Self-Assembling Protein Microarrays

  2004cited by this paper
• Functional protein microarrays for parallel characterisation of p53 mutants

  2004cited by this paper
• Optimizing spotting solutions for increased reproducibility of cDNA microarrays.

  2003cited by this paper
• Comprehensive Identification of Human bZIP Interactions with Coiled-Coil Arrays

  2003cited by this paper
• Proteome-scale purification of human proteins from bacteria

  2002cited by this paper
• Global Analysis of Protein Activities Using Proteome Chips

  2001cited by this paper
• Printing proteins as microarrays for high-throughput function determination.

  2000cited by this paper

• Serum profiling of the antibody response to HPV in women with or without abnormal cervical cytology undergoing cervical cancer screening

  2025cites this paper
• Humoral Response Profile in SARS-CoV-2 Infection: Differences across Virus Strains and Influenza.

  2025cites this paper
• The Latest Advances in Omics Technology for Assessing Tissue Damage: Implications for the Study of Sudden Cardiac Death

  2025cites this paper
• Deep learning based CETSA feature prediction cross multiple cell lines with latent space representation

  2024cites this paper
• Cell-Free Gene Expression: Methods and Applications

  2024cites this paper
• Streamlining assays of glycosyltransferases activity using in vitro GT-array (i-GT-ray) platform: Application to family GT37 fucosyltransferases

  2024cites this paper
• Cell-Int: a cell–cell interaction assay to identify native membrane protein interactions

  2024cites this paper
• Cell surface protein–protein interaction profiling for biological network analysis and novel target discovery

  2024cites this paper
• Screening Methods for Cervical Cancer

  2024cites this paper
• In Vitro GT-array (i-GT-ray), a Platform for Screening of Glycosyltransferase Activities and Protein–Protein Interactions

  2024cites this paper
• Specific protein interactions between rice members of the GT43 and GT47 families form various central cores of putative xylan synthase complexes.

  2024cites this paper
• Human antibody profiling technologies for autoimmune disease

  2023cites this paper
• Quantitative assessment of multiple pathogen exposure and immune dynamics at scale

  2023cites this paper
• Comparative Analysis of Antimicrobial Antibodies between Mild and Severe COVID-19

  2023cites this paper
• Advancements in Oncoproteomics Technologies: Treading toward Translation into Clinical Practice

  2023cites this paper
• Antibody responses to the host microbiome in chronic rhinosinusitis

  2022cites this paper
• Development of a protein microarray for profiling circulating autoantibodies in human diseases

  2022cites this paper
• Supramolecular protein assembly in cell-free protein synthesis system

  2022cites this paper
• SARS-CoV-2 Infection Triggers Auto-Immune Response in ARDS

  2022cites this paper
• CETSA Feature Based Clustering for Protein Outlier Discovery by Protein-to-Protein Interaction Prediction

  2022cites this paper
• Comparative microbiomics analysis of anti-microbial antibody response between lung cancer patients and control subjects with benign pulmonary nodules.

  2022cites this paper
• Programmable Synthesis of Biobased Materials Using Cell‐Free Systems

  2022cites this paper
• Multiomics Profiling of Alzheimer's Disease Serum for the Identification of Autoantibody Biomarkers.

  2021cites this paper
• A Quantitative Systems Approach to Define Novel Effects of Tumour p53 Mutations on Binding Oncoprotein MDM2

  2021cites this paper
• A highly predictive autoantibody-based biomarker panel for prognosis in early-stage NSCLC with potential therapeutic implications

  2021cites this paper
• Analysis of Protein-Protein Interactions by Protein Microarrays.

  2021cites this paper
• Proteomics for Understanding the Interaction Between Plant and Rhizospheric Microflora

  2021cites this paper
• Identification of Antibody Biomarker Using High-Density Nucleic Acid Programmable Protein Array.

  2021cites this paper
• Identification of anti-Epstein-Barr virus (EBV) antibody signature in EBV-associated gastric carcinoma

  2021cites this paper
• Deciphering Intracellular Signaling Pathways in Tumoral Pathologies.

  2021cites this paper
• Tracking the Antibody Immunome in Sporadic Colorectal Cancer by Using Antigen Self-Assembled Protein Arrays

  2021cites this paper
• A novel protein chip for simultaneous detection of antibodies against four epidemic swine viruses in China

  2020cites this paper
• Autoantibody profiling identifies predictive biomarkers of response to anti-PD1 therapy in cancer patients

  2020cites this paper
• Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology

  2020cites this paper
• Visualizing and Manipulating Biological Processes by Using HaloTag and SNAP‐Tag Technologies

  2020cites this paper
• Developments and Applications of Functional Protein Microarrays*

  2020cites this paper
• Technological Advances in Preclinical Drug Evaluation: the Role of -Omics Methods.

  2020cites this paper
• Advances in Microtechnology for Improved Cytotoxicity Assessment

  2020cites this paper
• Identification of tumor-associated antigens with diagnostic ability of colorectal cancer by in-depth immunomic and seroproteomic analysis.

  2020cites this paper
• Identification of tumor-associated antigens with diagnostic ability of 1 colorectal cancer by in-depth immunomic and seroproteomic analysis 2 3

  2020cites this paper
• The specific seroreactivity to ∆Np73 isoforms shows higher diagnostic ability in colorectal cancer patients than the canonical p73 protein

  2019cites this paper
• Discovery of an autoantibody signature for the early diagnosis of knee osteoarthritis: data from the Osteoarthritis Initiative

  2019cites this paper
• Bottom-up construction of complex biological systems with cell-free synthetic biology

  2019cites this paper
• Two novel protein chips for the detection of antibodies against porcine parvovirus

  2019cites this paper
• Approaches to identify extracellular receptor-ligand interactions.

  2019cites this paper
• INTEGRATING VIROLOGY AND PROTEOMICS TO COMPREHEND MECHANISMS OF INNATE IMMUNE SIGNALING DURING HERPESVIRUS INFECTION

  2019cites this paper
• Advances in high-throughput methods for the identification of virus receptors

  2019cites this paper
• Protein microarray technology: Assisting personalized medicine in oncology (Review)

  2019influential citation
• Sequencer Hacking Unlocks Quantitative Protein Studies.

  2019cites this paper
• Protein Microarrays

  2019cites this paper
• A decade of Nucleic Acid Programmable Protein Arrays (NAPPA) availability: News, actors, progress, prospects and access.

  2019cites this paper
• Review: Plant cell wall biochemical omics: The high-throughput biochemistry for polysaccharide biosynthesis.

  2019cites this paper
• A Systematic Workflow for Design and Computational Analysis of Protein Microarrays

  2019cites this paper
• HaloTag-based conjugation of proteins to barcoding-oligonucleotides

  2019cites this paper
• Electrochemical biosensors for autoantibodies in autoimmune and cancer diseases

  2019cites this paper
• A Systematic Analysis Workflow for High-Density Customized Protein Microarrays in Biomarker Screening.

  2018cites this paper
• High-throughput screening of biomolecules using cell-free gene expression systems

  2018cites this paper
• [Early detection and treatment of breast cancer].

  2018cites this paper
• Profiling Interactome Networks with the HaloTag-NAPPA In Situ Protein Array.

  2018cites this paper
• Screening Phage-Display Antibody Libraries Using Protein Arrays.

  2018cites this paper
• Expanding One-Pot Cell-Free Protein Synthesis and Immobilization for On-Demand Manufacturing of Biomaterials.

  2018cites this paper
• Exploring the protein-protein interaction landscape in plants.

  2018cites this paper
• A diagnostic autoantibody signature for primary cutaneous melanoma

  2018cites this paper
• Legionella effector AnkX interacts with host nuclear protein PLEKHN1

  2018cites this paper
• Advances in cell-free protein array methods

  2018cites this paper
• Discovering cellular protein-protein interactions: Technological strategies and opportunities.

  2018cites this paper
• Synthese von Peptiden, Peptoiden und Protein-ähnlichen Strukturen im µ Arrayformat

  2018cites this paper
• Development of array systems for molecular diagnostic assays

  2018cites this paper
• Microarrays as Platform for Multiplex Assays in Biomarker and Drug Discovery

  2018cites this paper
• Self-assembling functional programmable protein array for studying protein–protein interactions in malaria parasites

  2018cites this paper
• The Immunome of Colon Cancer: Functional In Silico Analysis of Antigenic Proteins Deduced from IgG Microarray Profiling

  2018cites this paper
• Identification of Antibody Against SNRPB, Small Nuclear Ribonucleoprotein-Associated Proteins B and B’, as an Autoantibody Marker in Crohn’s Disease using an Immunoproteomics Approach

  2017cites this paper
• rDNA Mediated Bioconjugates: Fusion Proteins and their Intended Use in Medicine.

  2017cites this paper
• Proteome-Scale Human Interactomics.

  2017cites this paper
• Automatic Identification and Quantification of Extra-Well Fluorescence in Microarray Images.

  2017cites this paper
• Multiplexed Nucleic Acid Programmable Protein Arrays

  2017cites this paper
• Autoantibody biomarkers for the detection of serous ovarian cancer.

  2017cites this paper
• Protein Arrays II: Antigen Arrays.

  2017cites this paper
• Decoding the anticancer activity of VO-clioquinol compound: the mechanism of action and cell death pathways in human osteosarcoma cells.

  2017cites this paper
• Discovering Protein-Protein Interactions Using Nucleic Acid Programmable Protein Arrays.

  2017cites this paper
• Serum Immune Profiling for Early Detection of Cervical Disease

  2017influential citation
• Technologies for Proteome-Wide Discovery of Extracellular Host-Pathogen Interactions

  2017cites this paper
• Formation of the Legionella-containing vacuole: phosphoinositide conversion, GTPase modulation and ER dynamics.

  2017cites this paper
• Methods for Selecting Phage Display Antibody Libraries.

  2017cites this paper
• The Expanding World of Small Molecule Microarrays.

  2017cites this paper
• Array-based Autoantibody Profiling and Epitope Mapping

  2017cites this paper
• Tracking the Antibody Immunome in Type 1 Diabetes Using Protein Arrays.

  2017cites this paper
• Identification of Antibody Targets for Tuberculosis Serology using High-Density Nucleic Acid Programmable Protein Arrays*

  2017cites this paper
• A Contra Capture Protein Array Platform for Studying Post-translationally Modified (PTM) Auto-antigenomes*

  2016cites this paper
• Activating transcription factor 6 derepression mediates neuroprotection in Huntington disease.

  2016influential citation
• Deciphering the effect of an oxovanadium(iv) complex with the flavonoid chrysin (VOChrys) on intracellular cell signalling pathways in an osteosarcoma cell line.

  2016cites this paper
• Programmable protein arrays for immunoprofiling HPV‐associated cancers

  2016cites this paper
• Proteomic mapping of p53 immunogenicity in pancreatic, ovarian, and breast cancers

  2016cites this paper
• Advancing translational research with next‐generation protein microarrays

  2016cites this paper
• The prostate cancer immunome: In silico functional analysis of antigenic proteins from microarray profiling with IgG

  2016cites this paper
• New era of integrated cancer biomarker discovery using reverse-phase protein arrays.

  2016cites this paper
• Mapping transcription factor interactome networks using HaloTag protein arrays

  2016influential citation
• Proteogenomics

  2016cites this paper
• Proteomic Monitoring of B Cell Immunity.

  2016cites this paper
• Antibody-Based Proteomics.

  2016cites this paper