Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate

No abstract is available for this paper.

• Publication year

  2001

• Venue

  EMBO Journal

• Publication date

  2001-12-03

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1093/emboj/20.23.6877PMID 11726523PMCID PMC125328
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• A Role for the RNase III Enzyme DCR-1 in RNA Interference and Germ Line Development in Caenorhabditis elegans

  2001cited by this paper
• Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing.

  2001cited by this paper
• Role for a bidentate ribonuclease in the initiation step of RNA interference

  2001cited by this paper
• Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells

  2001cited by this paper
• Gene silencing by double-stranded RNA.

  2001cited by this paper
• Self-assembly of mesoscopically ordered chromatic polydiacetylene/silica nanocomposites

  2001cited by this paper
• Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi.

  2001cited by this paper
• RNA Interference and Small Interfering RNAs

  2001cited by this paper
• ATP requirements and small interfering RNA structure in the RNA interference pathway.

  2001cited by this paper
• Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems

  2001cited by this paper
• RNA silencing as a plant immune system against viruses.

  2001cited by this paper
• RNA interference--2001.

  2001cited by this paper
• Gene silencing as an adaptive defence against viruses

  2001cited by this paper
• Argonaute2, a Link Between Genetic and Biochemical Analyses of RNAi

  2001cited by this paper
• Post-transcriptional gene silencing by double-stranded RNA

  2001cited by this paper
• A Cellular Function for the RNA-Interference Enzyme Dicer in the Maturation of the let-7 Small Temporal RNA

  2001cited by this paper
• RNA interference is mediated by 21- and 22-nucleotide RNAs.

  2001cited by this paper
• The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans

  2000cited by this paper
• An RNA-dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus.

  2000cited by this paper
• Control of developmental timing in Caenorhabditis elegans.

  2000cited by this paper
• Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III

  2000cited by this paper
• Functional genomic analysis of C. elegans chromosome I by systematic RNA interference

  2000cited by this paper
• Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA

  2000cited by this paper
• RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals.

  2000cited by this paper
• Inducible expression of double-stranded RNA directs specific genetic interference in Drosophila.

  2000cited by this paper
• Double-stranded RNA as a template for gene silencing.

  2000cited by this paper
• Non-coding RNA's: lightning strikes twice.

  2000cited by this paper
• Detailed characterization of the posttranscriptional gene-silencing-related small RNA in a GUS gene-silenced tobacco.

  2000cited by this paper
• Arabidopsis SGS2 and SGS3 genes are required for posttranscriptional gene silencing and natural virus resistance.

  2000cited by this paper
• Evidence that processed small dsRNAs may mediate sequence-specific mRNA degradation during RNAi in Drosophila embryos.

  2000cited by this paper
• RNAi analysis of genes expressed in the ovary of Caenorhabditis elegans.

  2000cited by this paper
• Molecular cloning and characterization of a novel human gene (HERNA) which encodes a putative RNA-helicase.

  2000cited by this paper
• Sensitive assay of RNA interference in Drosophila and Chinese hamster cultured cells using firefly luciferase gene as target

  2000cited by this paper
• Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways.

  2000cited by this paper
• The orphanin FQ/nociceptin gene: structure, tissue distribution of expression and functional implications obtained from knockout mice.

  2000cited by this paper
• Post-transcriptional gene silencing across kingdoms.

  2000cited by this paper
• Domains in gene silencing and cell differentiation proteins: the novel PAZ domain and redefinition of the Piwi domain.

  2000cited by this paper
• Functional anatomy of a dsRNA trigger: differential requirement for the two trigger strands in RNA interference.

  2000cited by this paper
• EGO-1 is related to RNA-directed RNA polymerase and functions in germ-line development and RNA interference in C. elegans.

  2000cited by this paper
• An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells

  2000cited by this paper
• A species of small antisense RNA in posttranscriptional gene silencing in plants.

  1999cited by this paper
• Targeted mRNA degradation by double-stranded RNA in vitro.

  1999cited by this paper
• RNA-triggered gene silencing.

  1999cited by this paper
• The lin-4 regulatory RNA controls developmental timing in Caenorhabditis elegans by blocking LIN-14 protein synthesis after the initiation of translation.

  1999cited by this paper
• Disruption of an RNA helicase/RNAse III gene in Arabidopsis causes unregulated cell division in floral meristems.

  1999cited by this paper
• Systemic spread of sequence-specific transgene RNA degradation in plants is initiated by localized introduction of ectopic promoterless DNA.

  1998cited by this paper
• Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans

  1998cited by this paper
• How cells respond to interferons.

  1998cited by this paper
• Systemic acquired silencing: transgene‐specific post‐transcriptional silencing is transmitted by grafting from silenced stocks to non‐silenced scions

  1997cited by this paper
• SHORT INTEGUMENT (SIN1), a gene required for ovule development in Arabidopsis, also controls flowering time.

  1996cited by this paper
• Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans.

  1993cited by this paper
• Nonlinear limits to the information capacity of optical ® bre communications

  year unknowncited by this paper

• Antagonism of RNA silencing in the yellow fever mosquito, Aedes aegypti, by the nsP2 protein of the prototype alphavirus.

  2026cites this paper
• Nanocarrier armor for siRNA delivery to address fracture risk in osteoporosis

  2026cites this paper
• Modern LC-MS strategies for comprehensive metabolite identification of therapeutic oligonucleotides.

  2026cites this paper
• The Valsa mali Effector VmSR1 Accelerates the Degradation of Tudor‐SN2 to Suppress RNA Silencing and Plant Immunity

  2025cites this paper
• RNA-based antibacterial agents: Mechanisms, functional insights, and challenges in therapeutic development

  2025cites this paper
• The Impact of Splicing Dysregulation on Neuromuscular Disorders and Current Neuromuscular Genetic Therapies

  2025cites this paper
• Chemical Modifications in Nucleic Acid Therapeutics.

  2025cites this paper
• Implications of Endogenous Small Regulatory RNAs on Gene Silencing in Mollusks

  2025cites this paper
• Implications of Endogenous Small Regulatory RNAs Survey in Mollusks on Gene Silencing Approaches

  2025cites this paper
• Nanoparticle-Mediated Delivery of siRNA: Targeting Gene Silencing in Cancer Therapy

  2025cites this paper
• The cleavage of dsRNA by StaufenC into siRNA affects the RNAi efficiency in the 28-spotted ladybeetle, Henosepilachna vigintioctopunctata.

  2025cites this paper
• The RNA-based immunotherapy and future perspectives

  2025cites this paper
• Small interfering RNA: From designing to therapeutic in cancer

  2025cites this paper
• GalNac-siRNA conjugate delivery technology promotes the treatment of typical chronic liver diseases

  2025cites this paper
• Recent Update on siRNA Therapeutics

  2025cites this paper
• Evaluation of different diets for dsRNA-based gene silencing assays in brinjal shoot and fruit borer, Leucinodes orbonalis

  2025cites this paper
• Pharmaceutical perspectives on oligonucleotide therapeutics and delivery systems.

  2025cites this paper
• Small RNA-mediated regulation of cross-kingdom gene expression in sugar beet genotypes resistant and susceptible to rhizomania

  2025cites this paper
• Landscape of small nucleic acid therapeutics: moving from the bench to the clinic as next-generation medicines

  2025cites this paper
• Cardiac Circular RNAs CDR1as, Circ-RCAN2, Circ-C12orf29 Show Cell-Specific Hypoxia-Induced Dysregulation and Distinct In Vitro Effects

  2025cites this paper
• RNA interference-based dsRNA application confers prolonged protection against rice blast and viral diseases, offering a scalable solution for enhanced crop disease management.

  2025cites this paper
• An overview of strategies and challenges adopted to silence BCR-ABL gene by small interfering RNA.

  2025cites this paper
• Delayed Repolarization Caused by hERG Block With Different Drug Modalities Can Be Detected in Stem Cell‐Derived Cardiomyocytes: Incubation Time Matters

  2025cites this paper
• Environmental factors affecting RNAi efficacy: Temperature but not plant cultivar influences Colorado potato beetle's response to insecticidal dsRNA

  2025cites this paper
• SNPD-siKRAS: siRNA specifically inhibits KRAS with a single-nucleotide mutation

  2025cites this paper
• AAV vectors for specific and efficient gene expression in microglia

  2025cites this paper
• Unveiling the translational and therapeutic potential of small interfering RNA molecules in combating SARS-CoV-2: A review.

  2025cites this paper
• Development of siRNA therapeutics to combat microbial infections: a bibliometric analysis

  2025cites this paper
• Small Interfering RNA Carriers for Oncotherapy: A Preclinical Overview

  2025cites this paper
• Dicer-like genes are essential for efficient plant infection in the rubber tree powdery mildew fungus Erysiphe quercicola.

  2025cites this paper
• Cell-Autonomous and Non-Cell-Autonomous Antiviral Immunity via siRNA-Directed RNAi in Drosophila melanogaster

  2025cites this paper
• Properties and synergistic effects of a nonionic backbone and aminoalkyl modified nucleosides in RNAs.

  2024cites this paper
• The PAZ domain of Aedes aegypti Dicer 2 is critical for accurate and high-fidelity size determination of virus-derived small interfering RNAs

  2024cites this paper
• The guide RNA sequence dictates the slicing kinetics and conformational dynamics of the Argonaute silencing complex

  2024cites this paper
• Advances in structural-guided modifications of siRNA.

  2024cites this paper
• RNAi-Induced Gene Silencing against Chikungunya and COVID-19: What Have We Learned So Far, and What Is the Way Forward?

  2024cites this paper
• Quantitative design of cell type-specific mRNA stability from microRNA expression data

  2024cites this paper
• Orthogonal and multiplexable genetic perturbations with an engineered prime editor and a diverse RNA array

  2024cites this paper
• Scientific Review of the Proarrhythmic Risks of Oligonucleotide Therapeutics: Are Dedicated ICH S7B/E14 Studies Needed for Low‐Risk Modalities?

  2024cites this paper
• Delivery Aspects for Implementing siRNA Therapeutics for Blood Diseases.

  2024cites this paper
• Rational nanoparticle design for efficient biomolecule delivery in plant genetic engineering.

  2024cites this paper
• The guide-RNA sequence dictates the slicing kinetics and conformational dynamics of the Argonaute silencing complex.

  2024cites this paper
• RNA interference-based strategies to control Botrytis cinerea infection in cultivated strawberry

  2024cites this paper
• Deciphering the Role of siRNA in Anxiety and Depression.

  2024cites this paper
• Molecular Characterization of Two Totiviruses from the Commensal Yeast Geotrichum candidum

  2023cites this paper
• Designing Cell Delivery Peptides and SARS-CoV-2-Targeting Small Interfering RNAs: A Comprehensive Bioinformatics Study with Generative Adversarial Network-Based Peptide Design and In Vitro Assays.

  2023cites this paper
• Pulmonary siRNA Delivery with Sophisticated Amphiphilic Poly(Spermine Acrylamides) for the Treatment of Lung Fibrosis

  2023cites this paper
• RNAi turns 25:contributions and challenges in insect science

  2023cites this paper
• RNAi-Based Therapy: Combating Shrimp Viral Diseases

  2023cites this paper
• A coronavirus-mimic mesoporous silica nanosystem enables efficient targeted delivery of siRNA for anti-SARS-CoV‐2

  2023cites this paper
• Towards precision medicine in non-alcoholic fatty liver disease

  2023cites this paper
• Interaction between Bombyx mori Cytoplasmic Polyhedrosis Virus NSP8 and BmAgo2 Inhibits RNA Interference and Enhances Virus Proliferation

  2023cites this paper
• Insights in piRNA targeting rules

  2023cites this paper
• Tweaking the Small Non-Coding RNAs to Improve Desirable Traits in Plant

  2023cites this paper
• Knockdown of Dcr1 and Dcr2 limits the lethal effect of C-factor in Chilo suppressalis.

  2023cites this paper
• Future Treatment Options and Regimens for Nonalcoholic Fatty Liver Disease.

  2023cites this paper
• mRNA Abundance of Neurogenic Factors Correlates with Hearing Capacity in Auditory Brainstem Nuclei of the Rat

  2023cites this paper
• Brain-derived extracellular vesicles promote bone-fat imbalance in Alzheimer's disease

  2023cites this paper
• Biochemistry-informed design selects potent siRNAs against SARS-CoV-2

  2023cites this paper
• Towards the Clinical Application of Gene Therapy for Genetic Inner Ear Diseases

  2023cites this paper
• An Artificial Small RNA Editor by Chimeric dsRNase with RNA Binding Protein

  2022cites this paper
• An Adenoviral Vector as a Versatile Tool for Delivery and Expression of miRNAs

  2022cites this paper
• RNAi-mediated silencing of an egg-specific gene Nllet1 results in hatch failure in the brown planthopper.

  2022cites this paper
• Thermal preconditioning in a reef-building coral alleviates oxidative damage through a BI-1 mediated antioxidant response

  2022cites this paper
• Therapeutic siRNA: State-of-the-Art and Future Perspectives

  2022cites this paper
• Chemical engineering of therapeutic siRNAs for allele-specific gene silencing in Huntington’s disease models

  2022cites this paper
• RNAi for Western Corn Rootworm Management: Lessons Learned, Challenges, and Future Directions

  2022cites this paper
• Catching Them Early: Framework Parameters and Progress for Prenatal and Childhood Application of Advanced Therapies

  2022cites this paper
• Positional effects of double-stranded RNAs targeting β-Actin gene affect RNA interference efficiency in Colorado potato beetle.

  2022cites this paper
• High-throughput biochemical profiling reveals functional adaptation of a bacterial Argonaute.

  2022cites this paper
• Molecular mechanisms underlying host-induced gene silencing

  2022cites this paper
• Prediction of putative potential siRNAs for inhibiting SARS-CoV-2 strains, including variants of concern and interest

  2022cites this paper
• Cell delivery peptides for small interfering RNAs targeting SARS-CoV-2 new variants through a bioinformatics and deep learning design

  2022cites this paper
• Conserved MicroRNAs in Human Nasopharynx Tissue Samples from Swabs Are Differentially Expressed in Response to SARS-CoV-2

  2022cites this paper
• Delivery of siRNA to Macrophages: Challenges and Opportunities

  2022cites this paper
• Chemical engineering of therapeutic siRNAs for allele-specific gene silencing in vivo in CNS

  2022cites this paper
• Disruption of tomato TGS machinery by ToLCNDV causes reprogramming of vascular tissue-specific TORNADO1 gene expression

  2022cites this paper
• A comprehensive update of siRNA delivery design strategies for targeted and effective gene silencing in gene therapy and other applications

  2022cites this paper
• Time Is a Critical Factor When Evaluating Oligonucleotide Therapeutics in hERG Assays

  2022influential citation
• RNAi-based pest control: Production, application and the fate of dsRNA

  2022cites this paper
• 6′-Sialylactose abolished lipopolysaccharide-induced inflammation and hyper-permeability in endothelial cells

  2022cites this paper
• Delivering the Promise of Gene Therapy with Nanomedicines in Treating Central Nervous System Diseases

  2022cites this paper
• Self-assembling short immunostimulatory duplex RNAs with broad-spectrum antiviral activity

  2022cites this paper
• A Tale of Two Lobsters—Transcriptomic Analysis Reveals a Potential Gap in the RNA Interference Pathway in the Tropical Rock Lobster Panulirus ornatus

  2022cites this paper
• CRISPR/Cas9 Genome-Editing Technology and Potential Clinical Application in Gastric Cancer

  2022cites this paper
• Rabies Prophylactic and Treatment Options: An In Vitro Study of siRNA- and Aptamer-Based Therapeutics

  2021cites this paper
• Antibody Conjugates for Targeted Therapy Against HIV-1 as an Emerging Tool for HIV-1 Cure

  2021cites this paper
• Host-Induced Gene Silencing of a Sclerotinia sclerotiorum oxaloacetate acetylhydrolase Using Bean Pod Mottle Virus as a Vehicle Reduces Disease on Soybean

  2021cites this paper
• Genome editing and RNA interference technologies in plants

  2021cites this paper
• RNA interference as a promising strategy for plant disease management

  2021cites this paper
• siRNA Therapeutics for the Therapy of COVID-19 and Other Coronaviruses

  2021cites this paper
• Disruption of Tomato TGS Machinery by ToLCNDV Causes Reprogramming of Vascular Tissue Specific TORNADO1 Gene Expression

  2021cites this paper
• RNA Interference in Aquaculture: A Small Tool for Big Potential.

  2021cites this paper
• Crowdsourced Identification of Potential Target Genes for CTV Induced Gene Silencing for Controlling the Citrus Greening Vector Diaphorina citri

  2021cites this paper
• siRNA enrichment in Argonaute 2-depleted Blattella germanica

  2021cites this paper
• eScholarship@UMassChan eScholarship@UMassChan

  2021cites this paper
• A conserved role of the duplicated Masculinizer gene in sex determination of the Mediterranean flour moth, Ephestia kuehniella

  2021cites this paper
• Advances in siRNA delivery strategies for the treatment of MDR cancer.

  2021cites this paper
• RNA therapeutics in ophthalmology - translation to clinical trials.

  2021cites this paper
• The Interplay Between Viruses and RNAi Pathways in Insects.

  2021cites this paper