Optimal reference genes for real‐time quantitative PCR and the expression of sigma factors in Acidithiobacillus caldus under various conditions

Acidithiobacillus caldus is an important sulphur‐oxidizing bacterium that plays crucial roles in the bioleaching industry. This study aims to analyse the optimal reference gene for real‐time quantitative PCR (RT‐qPCR) under different conditions and investigate the transcription levels of the sigma factor genes in the stress response.

• Publication year

  2021

• Venue

  Journal of Applied Microbiology

• Publication date

  2021-03-23

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1111/jam.15085PMID 33754423
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Essential Role of σ Factor RpoF in Flagellar Biosynthesis and Flagella-Mediated Motility of Acidithiobacillus caldus

  2019cited by this paper
• Selection of the optimal reference genes for expression analyses in different materials of Eriobotrya japonica

  2019cited by this paper
• Discovery of a new subgroup of sulfur dioxygenases and characterization of sulfur dioxygenases in the sulfur metabolic network of Acidithiobacillus caldus

  2017cited by this paper
• The Two-Component System RsrS-RsrR Regulates the Tetrathionate Intermediate Pathway for Thiosulfate Oxidation in Acidithiobacillus caldus

  2016influential reference
• The σ54-dependent two-component system regulating sulfur oxidization (Sox) system in Acidithiobacillus caldus and some chemolithotrophic bacteria

  2016cited by this paper
• Bacterial reference genes for gene expression studies by RT-qPCR: survey and analysis

  2015cited by this paper
• Elucidating the function of the RpoS regulon.

  2014cited by this paper
• Acidithiobacillus caldus Sulfur Oxidation Model Based on Transcriptome Analysis between the Wild Type and Sulfur Oxygenase Reductase Defective Mutant

  2012cited by this paper
• Unraveling the Acidithiobacillus caldus complete genome and its central metabolisms for carbon assimilation.

  2011cited by this paper
• Sulfur Metabolism in the Extreme Acidophile Acidithiobacillus Caldus

  2011cited by this paper
• Housekeeping genes; expression levels may change with density of cultured cells.

  2010cited by this paper
• The primary transcriptome of the major human pathogen Helicobacter pylori

  2010cited by this paper
• Selection and evaluation of reference genes for improved interrogation of microbial transcriptomes: case study with the extremophile Acidithiobacillus ferrooxidans

  2009cited by this paper
• Draft Genome Sequence of the Extremely Acidophilic Bacterium Acidithiobacillus caldus ATCC 51756 Reveals Metabolic Versatility in the Genus Acidithiobacillus

  2009cited by this paper
• Ab initio construction of a eukaryotic transcriptome by massively parallel mRNA sequencing

  2009cited by this paper
• Real-time PCR analysis of the heat-shock response of Acidithiobacillus ferrooxidans ATCC 23270.

  2009cited by this paper
• Response of Acidithiobacillus ferrooxidans ATCC 23270 gene expression to acid stress

  2008cited by this paper
• Sigma factors in Pseudomonas aeruginosa.

  2008cited by this paper
• On the nature of human housekeeping genes.

  2008cited by this paper
• Identification of suitable internal controls to study expression of a Staphylococcus aureus multidrug resistance system by quantitative real-time PCR.

  2007cited by this paper
• Evaluation of DNA microarray results with quantitative gene expression platforms

  2006cited by this paper
• Quantification of mRNA using real-time RT-PCR

  2006cited by this paper
• Faculty Opinions recommendation of Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis.

  2006cited by this paper
• Genome-Wide Identification and Testing of Superior Reference Genes for Transcript Normalization in Arabidopsis1[w]

  2005cited by this paper
• Standardization strategy for quantitative PCR in human seminoma and normal testis.

  2005cited by this paper
• Normalization of Real-Time Quantitative Reverse Transcription-PCR Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data Sets

  2004cited by this paper
• Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations

  2004cited by this paper
• Determination of the Core of a Minimal Bacterial Gene Set

  2004cited by this paper
• Enumeration and Characterization of Acidophilic Microorganisms Isolated from a Pilot Plant Stirred-Tank Bioleaching Operation

  2003cited by this paper
• Gene Expression Profile of Campylobacter jejuni in Response to Growth Temperature Variation

  2003cited by this paper
• Post-analysis follow-up and validation of microarray experiments

  2002cited by this paper
• Control genes and variability: absence of ubiquitous reference transcripts in diverse mammalian expression studies.

  2002cited by this paper
• Signal Transduction and Regulatory Mechanisms Involved in Control of the σS (RpoS) Subunit of RNA Polymerase

  2002cited by this paper
• Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

  2002influential reference
• Control genes in quantitative molecular biological techniques: the variability of invariance.

  2001cited by this paper
• Beta-actin--an unsuitable internal control for RT-PCR.

  2001cited by this paper
• Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

  2001cited by this paper
• Quantification on the LightCycler

  2001cited by this paper
• Further defining housekeeping, or "maintenance," genes Focus on "A compendium of gene expression in normal human tissues".

  2001cited by this paper
• Characteristics of attachment and growth of Thiobacillus caldus on sulphide minerals: a chemotactic response to sulphur minerals?

  2000cited by this paper
• Control selection for RNA quantitation.

  2000cited by this paper
• Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR.

  2000cited by this paper
• Direct comparison of GAPDH, beta-actin, cyclophilin, and 28S rRNA as internal standards for quantifying RNA levels under hypoxia.

  1999cited by this paper
• Potential Role of Thiobacillus caldus in Arsenopyrite Bioleaching

  1999cited by this paper
• Genome-wide expression profiling in Escherichia coli K-12.

  1999cited by this paper
• Housekeeping genes as internal standards: use and limits.

  1999cited by this paper
• Industrial practice and the biology of leaching of metals from ores The 1997 Pan Labs Lecture

  1998cited by this paper
• Promoter selectivity of Escherichia coli RNA polymerase sigmaF holoenzyme involved in transcription of flagellar and chemotaxis genes

  1997cited by this paper
• Multiple Serotypes of the Moderate Thermophile Thiobacillus caldus, a Limitation of Immunological Assays for Biomining Microorganisms

  1996cited by this paper
• Characterization of Thiobacillus caldus sp. nov., a moderately thermophilic acidophile.

  1994cited by this paper
• Analysis of the Streptomyces coelicolor sigE gene reveals the existence of a subfamily of eubacterial RNA polymerase sigma factors involved in the regulation of extracytoplasmic functions.

  1994cited by this paper
• In a class of its own--the RNA polymerase sigma factor sigma 54 (sigma N).

  1993cited by this paper
• In a class of its own — the RNA polymerase sigma factor σ;54 (σN)

  1993cited by this paper
• Identification of a central regulator of stationary‐phase gene expression in Escherichia coli

  1991cited by this paper
• RNA polymerase sigma-factor and the selection of initiation site.

  1970cited by this paper
• RNA Polymerase σ-Factor and the Selection of Initiation Site

  1970cited by this paper
• Bmc Molecular Biology Identification, Characterization and Expression of Novel Sex Hormone Binding Globulin Alternative First Exons in the Human Prostate

  year unknowninfluential reference
• Bmc Molecular Biology Identification and Validation of Reference Genes for Quantitative Rt-pcr Normalization in Wheat

  year unknowncited by this paper
• Bmc Molecular Biology Validation of Internal Control for Gene Expression Study in Soybean by Quantitative Real-time Pcr

  year unknowncited by this paper

• A one-step route for the conversion of Cd waste into CdS quantum dots by Acidithiobacillus sp. via unique biosynthesis pathways

  2024cites this paper