Single-Molecular Förster Resonance Energy Transfer Measurement on Structures and Interactions of Biomolecules

Single-molecule Förster resonance energy transfer (smFRET) inherits the strategy of measurement from the effective “spectroscopic ruler” FRET and can be utilized to observe molecular behaviors with relatively high throughput at nanometer scale. The simplicity in principle and configuration of smFRET make it easy to apply and couple with other technologies to comprehensively understand single-molecule dynamics in various application scenarios. Despite its widespread application, smFRET is continuously developing and novel studies based on the advanced platforms have been done. Here, we summarize some representative examples of smFRET research of recent years to exhibit the versatility and note typical strategies to further improve the performance of smFRET measurement on different biomolecules.

• Publication year

  2021

• Venue

  Micromachines

• Publication date

  2021-04-27

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.3390/mi12050492PMID 33925350PMCID 8145425
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Single-molecule FRET imaging of GPCR dimers in living cells

  2021cited by this paper
• Single bacterial resolvases first exploit, then constrain intrinsic dynamics of the Holliday junction to direct recombination

  2021cited by this paper
• Single-molecule FRET on its way to structural biology in live cells

  2021cited by this paper
• Supporting Information Single-molecule Förster Resonance Energy Transfer-Based Photosensitizer for Synergistic Photodynamic/Photothermal Therapy

  2021cited by this paper
• Super-resolution Imaging of Energy Transfer by Intensity-Based STED-FRET.

  2021cited by this paper
• Interrogating accessibility of telomeric sequences with FRET-PAINT: evidence for length-dependent telomere compaction

  2021cited by this paper
• One-step G-quadruplex-based fluorescence resonance energy transfer sensing method for ratiometric detection of uracil-DNA glycosylase activity.

  2021cited by this paper
• Conformational changes in the Ebola virus membrane fusion machine induced by pH, Ca2+, and receptor binding

  2020cited by this paper
• A mechanism for the extension and unfolding of parallel telomeric G-quadruplexes by human telomerase at single-molecule resolution

  2020cited by this paper
• Single-molecule fluorescence resonance energy transfer and its biomedical applications

  2020cited by this paper
• Quantum dots for Förster Resonance Energy Transfer (FRET)

  2020cited by this paper
• Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein

  2020cited by this paper
• Conformational ensembles of an intrinsically disordered protein consistent with NMR, SAXS and single-molecule FRET.

  2020cited by this paper
• Single-molecule view of coordination in a multi-functional DNA polymerase

  2020cited by this paper
• Untangling the Conformational Polymorphism of Disordered Proteins Associated With Neurodegeneration at the Single-Molecule Level

  2020cited by this paper
• Single-molecule visualisation of DNA G-quadruplex formation in live cells

  2020cited by this paper
• Reliable FRET-ON imaging of telomerase in living cells by a tetrahedral DNA nanoprobe integrated with structure-switchable molecular beacon

  2020cited by this paper
• Dynamics of the nucleosomal histone H3 N-terminal tail revealed by high precision single-molecule FRET

  2020cited by this paper
• Real-Time Conformational Dynamics of SARS-CoV-2 Spikes on Virus Particles

  2020cited by this paper
• Kinetics of the conformational cycle of Hsp70 reveals the importance of the dynamic and heterogeneous nature of Hsp70 for its function

  2020cited by this paper
• SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor

  2020cited by this paper
• In silico method for selecting residue pairs for single-molecule microscopy and spectroscopy

  2020cited by this paper
• FRET‐MC: A fluorescence melting competition assay for studying G4 structures in vitro

  2020cited by this paper
• Conformational and migrational dynamics of slipped-strand DNA three-way junctions containing trinucleotide repeats

  2020cited by this paper
• Chirality-Dependent Amino Acid Modulation of RNA Folding.

  2020cited by this paper
• Encoding multiple virtual signals in DNA barcodes with single-molecule FRET

  2020cited by this paper
• Real-time Conformational Dynamics of SARS-CoV-2 Spikes on Virus Particles

  2020cited by this paper
• SDS-induced multi-stage unfolding of a small globular protein through different denatured states revealed by single-molecule fluorescence

  2020cited by this paper
• High-Resolution Single-Molecule FRET via DNA eXchange (FRET X)

  2020cited by this paper
• In-cell single-molecule FRET measurements reveal three conformational state changes in RAF protein.

  2020cited by this paper
• Automated E‐FRET microscope for dynamical live‐cell FRET imaging

  2019cited by this paper
• Chromatin nanoscale compaction in live cells visualized by acceptor‐to‐donor ratio corrected Förster resonance energy transfer between DNA dyes

  2019cited by this paper
• Homologous Recombination under the Single-Molecule Fluorescence Microscope

  2019cited by this paper
• Genetically Encoded FRET Biosensor for Visualizing EphA4 Activity in Different Compartments of the Plasma Membrane.

  2019cited by this paper
• Detection of Mg2+-dependent, coaxial stacking rearrangements in a bulged three-way DNA junction by single-molecule FRET.

  2019cited by this paper
• Ebola Virus Entry: From Molecular Characterization to Drug Discovery

  2019cited by this paper
• Site-Specific Fluorophore Labeling of Guanosines in RNA G-Quadruplexes

  2019cited by this paper
• Protein misfolding, aggregation and mechanism of amyloid cytotoxicity: An overview and therapeutic strategies to inhibit aggregation.

  2019cited by this paper
• Polyphosphate initiates tau aggregation through intra- and intermolecular scaffolding

  2019cited by this paper
• Studying Structural Dynamics of Potassium Channels by Single-Molecule FRET.

  2018cited by this paper
• High precision FRET studies reveal reversible transitions in nucleosomes between microseconds and minutes

  2018cited by this paper
• A minimal helical-hairpin motif provides molecular-level insights into misfolding and pharmacological rescue of CFTR

  2018cited by this paper
• Three-Color Single-Molecule FRET and Fluorescence Lifetime Analysis of Fast Protein Folding.

  2018cited by this paper
• FRET probes for measuring sphingolipid metabolizing enzyme activity.

  2018cited by this paper
• Comparative performance of airyscan and structured illumination superresolution microscopy in the study of the surface texture and 3D shape of pollen

  2018cited by this paper
• Intrinsic Disorder and Posttranslational Modifications: The Darker Side of the Biological Dark Matter

  2018cited by this paper
• Single-Molecule Imaging Reveals Conformational Manipulation of Holliday Junction DNA by the Junction Processing Protein RuvA.

  2018cited by this paper
• Toward dynamic structural biology: Two decades of single-molecule Förster resonance energy transfer

  2018cited by this paper
• Combining gold nanoparticle antennas with single-molecule fluorescence resonance energy transfer (smFRET) to study DNA hairpin dynamics.

  2018cited by this paper
• Commonly used FRET fluorophores promote collapse of an otherwise disordered protein

  2018cited by this paper
• Phasor histone FLIM-FRET microscopy quantifies spatiotemporal rearrangement of chromatin architecture during the DNA damage response

  2018cited by this paper
• Resolution of the Holliday junction recombination intermediate by human GEN1 at the single-molecule level

  2018cited by this paper
• Observing Single-Molecule Dynamics at Millimolar Concentrations.

  2017cited by this paper
• Nucleosome mobility and the regulation of gene expression: Insights from single‐molecule studies

  2017cited by this paper
• Unfolding of core nucleosomes by PARP-1 revealed by spFRET microscopy

  2017cited by this paper
• Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements

  2017cited by this paper
• Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2

  2017cited by this paper
• Single-molecule imaging and tracking of molecular dynamics in living cells

  2017cited by this paper
• Emerging roles of linker histones in regulating chromatin structure and function

  2017cited by this paper
• A single-molecule FRET sensor for monitoring DNA synthesis in real time.

  2017cited by this paper
• Ensemble and single-molecule FRET studies of protein synthesis.

  2017cited by this paper
• Understanding the FRET Signatures of Interacting Membrane Proteins*

  2017cited by this paper
• Single-molecule FRET-Rosetta reveals RNA structural rearrangements during human telomerase catalysis

  2017cited by this paper
• Substrate preference of Gen endonucleases highlights the importance of branched structures as DNA damage repair intermediates

  2017cited by this paper
• GXXXG-Mediated Parallel and Antiparallel Dimerization of Transmembrane Helices and Its Inhibition by Cholesterol: Single-Pair FRET and 2D IR Studies.

  2017cited by this paper
• Metal ion induced heterogeneity in RNA folding studied by smFRET

  2016cited by this paper
• Understanding the FRET Signatures of Interacting Membrane Proteins.

  2016cited by this paper
• Observing single FoF1-ATP synthase at work using an improved fluorescent protein mNeonGreen as FRET donor

  2016cited by this paper
• A functional role for intrinsic disorder in the tau-tubulin complex

  2016cited by this paper
• Amino Acid Specific Effects on RNA Tertiary Interactions: Single-Molecule Kinetic and Thermodynamic Studies.

  2016cited by this paper
• Single-molecule fluorescence resonance energy transfer in molecular biology.

  2016cited by this paper
• A Guide to Fluorescent Protein FRET Pairs

  2016cited by this paper
• smFRET and DEER Distance Measurements as Applied to Disordered and Structured Proteins

  2016cited by this paper
• Ebola Virus Entry: A Curious and Complex Series of Events

  2015cited by this paper
• Fast flow microfluidics and single-molecule fluorescence for the rapid characterization of α-synuclein oligomers.

  2015cited by this paper
• Adenoviral transduction of FRET-based biosensors for cAMP in primary adult mouse cardiomyocytes.

  2015cited by this paper
• Cholesterol-induced lipophobic interaction between transmembrane helices using ensemble and single-molecule fluorescence resonance energy transfer.

  2015cited by this paper
• Kinetic and thermodynamic origins of osmolyte-influenced nucleic acid folding.

  2015cited by this paper
• Dual fluorescent deoxyguanosine mimics for FRET detection of G-quadruplex folding.

  2015cited by this paper
• Probing the kinetic and thermodynamic consequences of the tetraloop/tetraloop receptor monovalent ion-binding site in P4-P6 RNA by smFRET.

  2015cited by this paper
• Heterogeneity of single molecule FRET signals reveals multiple active ribosome subpopulations

  2014cited by this paper
• Advances in live-cell single-particle tracking and dynamic super-resolution imaging.

  2014cited by this paper
• Using sm-FRET and denaturants to reveal folding landscapes.

  2014cited by this paper
• HIV-1 DIS stem loop forms an obligatory bent kissing intermediate in the dimerization pathway

  2014cited by this paper
• The FRET signatures of noninteracting proteins in membranes: simulations and experiments.

  2014cited by this paper
• Different Fluorophore Labeling Strategies and Designs Affect Millisecond Kinetics of DNA Hairpins

  2014cited by this paper
• Single-Molecule FRET Studies of HIV TAR–DNA Hairpin Unfolding Dynamics

  2014cited by this paper
• High performance, LED powered, waveguide based total internal reflection microscopy

  2013cited by this paper
• Kinetic Partitioning Modulates Human Telomere DNA G-Quadruplex Structural Polymorphism

  2013cited by this paper
• Histone‐ and DNA sequence‐dependent stability of nucleosomes studied by single‐pair FRET

  2013cited by this paper
• Monitoring the activity of single translocons.

  2013cited by this paper
• Elucidating the native architecture of the YidC: ribosome complex.

  2013cited by this paper
• Identification and super-resolution imaging of ligand-activated receptor dimers in live cells

  2013cited by this paper
• Direct Observation of the Interconversion of Normal and Toxic Forms of α-Synuclein

  2012cited by this paper
• Hidden complexity in the isomerization dynamics of Holliday junctions.

  2012cited by this paper
• Localizing internal friction along the reaction coordinate of protein folding by combining ensemble and single-molecule fluorescence spectroscopy

  2012cited by this paper
• Quantitative intensity-based FRET approaches--a comparative snapshot.

  2012cited by this paper
• A rule of seven in Watson-Crick base pairing of mismatched sequences

  2012cited by this paper
• Fluorescence characterization of the transfer RNA-like domain of transfer messenger RNA in complex with small binding protein B.

  2012cited by this paper
• Determination of interaction kinetics between the T cell receptor and peptide-loaded MHC class II via single-molecule diffusion measurements.

  2012cited by this paper

• Single Molecule Kinetic Fingerprinting of Glycans on IgA1 Antibodies.

  2025cites this paper
• Spectroscopic and Computational Analysis of 5PPD-CeO2: Insights from Fluorescence Quenching and FRET Studies

  2025cites this paper
• Use of AI-methods over MD simulations in the sampling of conformational ensembles in IDPs

  2025cites this paper
• Status, Challenges, and future trends in fluorescent sensors for Point-of-Care food safety assessment

  2025cites this paper
• Smart Luminescent Materials for Emerging Sensors: Fundamentals and Advances.

  2025cites this paper
• The effect of macromolecular crowding on protein misfolding as well as oxidative aggregation and its corresponding mechanisms under carbamylation conditions.

  2025cites this paper
• The use of single-molecule FRET for the characterization of Holliday junctions containing human telomeric DNA: a methodological approach for nanoscale distance, mobility, and stability measurements

  2024cites this paper
• Unraveling the complexity: Advanced methods in analyzing DNA, RNA, and protein interactions.

  2024cites this paper
• Insight into Single-Molecule Imaging Techniques for the Study of Prokaryotic Genome Maintenance

  2024cites this paper
• FRETing about the details: Case studies in the use of a genetically encoded fluorescent amino acid for distance‐dependent energy transfer

  2023cites this paper
• Current Advances in Nanotechnology for the Next Generation of Sequencing (NGS)

  2023cites this paper
• Probing the binding of morin with alpha-2-macroglobulin using multi-spectroscopic and molecular docking approach

  2023cites this paper
• Principles and Applications of Resonance Energy Transfer Involving Noble Metallic Nanoparticles

  2023cites this paper
• A comparative study of conventional FRET and light harvesting properties of Rh-110/Rh-6G and Rh-19/Rh-B organic dye pairs impregnated in sol-gel glasses

  2023cites this paper
• An overview of single-molecule techniques and applications in the study of nucleic acid structure and function.

  2022cites this paper
• Tracking Heme-Protein Interactions in Healthy and Pathological Human Serum in Native Conditions by Miniaturized FFF-Multidetection

  2022cites this paper
• Environmentally sensitive fluorescent nucleoside analogues as probes for nucleic acid – protein interactions: molecular design and biosensing applications

  2022cites this paper
• Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features.

  2022cites this paper
• Molecular Brightness Approach for FRET Analysis of Donor-Linker-Acceptor Constructs at the Single Molecule Level: A Concept

  2021cites this paper
• Methodologies for Measuring Protein Trafficking across Cellular Membranes.

  2021cites this paper
• Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes.

  2021cites this paper
• Distance-Dependent Fluorescence Resonance Energy Transfer Enhancement on Nanoporous Gold

  2021cites this paper
• Nano-assembly and optical properties of difluoroboron dibenzoylmethane-polysilane

  2021cites this paper