New colorimetric method for the quantitative estimation of phospholipids without acid digestion.

A unique colorimetric method for the quantitative determination of phospholipids that does not involve the acid digestion of the lipid is described. The phospholipids, after separation by thin-layer chromatography and elution from the silica gel, are heated with a chromogenic solution that is a modification of a spray reagent formulated by Vaskovsky and Kostetsky (1968, J. Lipid Res., 9: 396). The absorbance of the colored complex was read at 710 nm, and it followed Beer's law in the range of 1-10 micro g of phospholipid phosphorus.

• Publication year

  1973

• Venue

  Journal of Lipid Research

• Publication date

  1973-11-01

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1016/S0022-2275(20)36853-XPMID 4742564
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Determination of lipid phosphorus in the nanomolar range.

  1970cited by this paper
• Quantitative determination of phosphorus in soybean lipids.

  1969cited by this paper
• Modified spray for the detection of phospholipids on thin-layer chromatograms.

  1968cited by this paper
• The chemical composition of eggs.

  1966cited by this paper
• Qnantitatively analysis of phospholipids by thin-layer chromatography☆

  1965cited by this paper
• A sensitive method for phosphorus in lipids

  1965cited by this paper
• Quantitative analysis of phospholipids by thin-layer chromatography.

  1964cited by this paper
• Analysis of lipids from fresh and preserved adult human brains.

  1962cited by this paper
• Phosphorus assay in column chromatography.

  1959cited by this paper
• Microdetermination of Phosphorus

  1956cited by this paper

• Nutritional profiling of red seaweeds Grateloupia turuturu and Porphyra umbilicalis: literature-based insights into their potential for novel applications and partial replacement of conventional agricultural crops

  2025cites this paper
• Sustainable Recovery of Valuable Constituents from Octopus (Octopus vulgaris) Cooking Liquor

  2025cites this paper
• Photodynamic Inactivation As a Novel Approach for Seabass Decontamination: Effectiveness of TMPyP and Methylene Blue Treatments

  2025cites this paper
• Effect of Phosphorus Deprivation on Fatty Acid Synthesis in Scenedesmus subspicatus Microalgae from Rostherne Mere

  2024cites this paper
• Influence of Cuttlefish-Ink Extract on Canned Golden Seabream (Sparus aurata) Quality

  2024cites this paper
• The Presence of Bioactive Compounds in European Eel (Anguilla anguilla) Skin: A Comparative Study with Edible Tissue

  2024cites this paper
• Squid discards as a source of phospholipid- and tocopherol-concentrates by employment of non-halogenated solvents

  2024cites this paper
• Low-Toxicity Solvents for the Extraction of Valuable Lipid Compounds from Octopus (Octopus vulgaris) Waste

  2023cites this paper
• Yield Enhancement of Valuable Lipid Compounds from Squid (Doryteuthis gahi) Waste by Ethanol/Acetone Extraction

  2023cites this paper
• COMPARATIVE STUDIES ON THE PROXIMATE AND LIPID CONTENTS IN PROCESSED FISH SESAME AND PIGEON PEA FLOURS

  2023cites this paper
• Influence of Different Previous Frozen Holding Periods on the Canned Fish Quality

  2023cites this paper
• Comparative Study of Bioactive Lipid Extraction from Squid (Doryteuthis gahi) by-Products by Green Solvents

  2022cites this paper
• Antioxidant Effect of Octopus Byproducts in Canned Horse Mackerel (Trachurus trachurus) Previously Subjected to Different Frozen Storage Times

  2022cites this paper
• Phytochemical analysis and antimicrobial activity of Aframomum chrysanthum seed extracts

  2021cites this paper
• Physical Properties, Chemical Composition and Nutritional Quality Potentials of Lonchocarpus sericeus (Cube root) Seeds and Seed Oil

  2021cites this paper
• LIPID CHARACTERISTICS OF MANGO SEED KERNEL OIL AS AFFECTED BY DIFFERENT RIPENING STAGES OF FRUIT

  2021cites this paper
• Nutritional and Healthy Value of Chemical Constituents Obtained from Patagonian Squid (Doryteuthis gahi) By-Products Captured at Different Seasons

  2021cites this paper
• A simple spectrophotometric method to determine phytic acid in poultry wastewater without acid digestion

  2020cites this paper
• Biochemical Composition and Energy Strategy Along the Reproductive Cycle of Female Octopus vulgaris in Galician Waters (NW Spain)

  2020cites this paper
• Analytical Methods for Determination of Phytic Acid and Other Inositol Phosphates: A Review

  2020cites this paper
• Vitamin E, Phospholipid, and Phytosterol Contents of Parkia biglobosa and Citrullus colocynthis Seeds and Their Potential Applications to Human Health

  2019cites this paper
• Preservative Effect of Algae Extracts on Lipid Composition and Rancidity Development in Brine‐Canned Atlantic Chub Mackerel ( Scomber colias )

  2019cites this paper
• Chemical Composition and Nutritional Value of Different Seaweeds from the West Algerian Coast

  2019cites this paper
• Nutritional Fortification of Sunflower Meal by Bacillus Subtilis ATCC PTA-6737 Fermentation

  2018cites this paper
• Desiccation-induced ROS accumulation and lipid catabolism in recalcitrant Madhuca latifolia seeds

  2018influential citation
• The chemical composition of different edible locations (central and edge muscles) of flat fish (Lepidorhombus whiffiagonis)

  2018cites this paper
• Properties of Liposomes Containing Natural and Synthetic Lipids Formed by Microfluidic Mixing

  2018cites this paper
• Comparative Lipid Composition Study in Farmed and Wild Blackspot Seabream (Pagellus bogaraveo)

  2018cites this paper
• (Coupling TLC Chromatography and MALDI Mass Spectrometry for Lipids Analysis & Identification)

  2018cites this paper
• The Lipid Composition Profiles as Contributed by the Whole Organism, Flesh and Shell of Pandalus borealis Shrimp and their Nutritional Values

  2017cites this paper
• Lipid composition of Basella alba and Basella rubra leaves consumed in South- Western Nigeria: Nutritional implications

  2017cites this paper
• Modulation in arsenic-induced lipid catabolism in Glycine max using proline, 24-epibrassinolide and diphenylene iodonium

  2017cites this paper
• A Comparative Study of Lipid Composition of an Undervalued Crustacean (Munida spp.) Captured in Winter and Summer

  2017cites this paper
• Studies on the Lipid Composition of Two Types of African Palm Grubs Consumed in Nigeria

  2017cites this paper
• LIPID PROFILE OF Lanistes libycus EDIBLE PORTION (MORELET, 1848)

  2017cites this paper
• Protective Effect of Onion Wine on Alcoholic Fatty Liver in Rats

  2016cites this paper
• A selective calix[6]arene-based fluorescent chemosensor for phosphatidylcholine type lipids.

  2016cites this paper
• Effects of roasting and cooking processes on the lipids composition of raw wholeseed flour of African breadfruit ( Treculia africana )

  2016influential citation
• Chemical composition of the leaves of tea bush (Ocimum gratissium L.)

  2015cites this paper
• The Relationship in the Lipid Composition in Raw, Steeped and Germinated Wheat (Triticum durum) Grains

  2015cites this paper
• Citrus Seeds Oils as Sources of Quality Edible Oils

  2015cites this paper
• A Comparative Assessment of the Fatty Acid and Phospholipid Composition of Irvingia Gabonensis (African Wild Mango) and Citrullus Lanatus (Water Melon) Seed Oils

  2015cites this paper
• Lipid composition of the brains of she-goat and castrated goat consumed in Ekiti State, Nigeria

  2015cites this paper
• Lipid Compositions of Three Varieties of Melon Seeds Flour: Dietary and Health Implications

  2015cites this paper
• Comparative Study of the Lipid and Fatty Acid Composition of Two Shell Fish : Lagoon and Fresh Water Crabs

  2015cites this paper
• Variability in permeability and integrity of cell membrane and depletion of food reserves in neem (Azadirachta indica) seeds from trees of different age classes

  2014cites this paper
• BMR Biotechnology-Lipid Composition of Afromomum melegueta, Zingiber officinale, Afromomum melegueta & Xylopic aethiopica

  2014cites this paper
• Characterization of Membrane Lipidome Changes in Pseudomonas aeruginosa during Biofilm Growth on Glass Wool

  2014cites this paper
• Erratum to: Does the trophic habitat influence the biochemical quality of the gonad of Octopus vulgaris? Stable isotopes and lipid class contents as bio-indicators of different life-cycle strategies

  2014cites this paper
• Bone Marrow: A Source of Nutritionally Valuable Fats as Typified in the Femur of Ram and Bull

  2014cites this paper
• Quantitative changes in lipids and carbohydrates of temporal workers and drones in Apis cerana indica (Fabricius)

  2014cites this paper
• Effect of Processing on Fatty Acid and Phospholipid Compositions of Harms ( Brachystegia eurycoma ) Seed Grown in Nigeria

  2014cites this paper
• Lipid composition of three organs of Hippotragus equinus ( Roan Antelope )

  2013cites this paper
• The effect of fermentation on the dietary quality of lipids from African locust bean ( Parkia biglobosa ) seeds

  2013cites this paper
• Lipid Composition of the Seeds of Three Types of Chillies Consumed in Nigeria

  2013cites this paper
• Effect of 50% Hydro-Ethanolic Leaf Extracts of Ruellia Tuberosa L. and Dipteracanthus Patulus (Jacq.) on Lipid Profile in Alloxan Induced Diabetic Rats

  2013cites this paper
• Effects of Roasting on the Lipid Quality of Raw Terminalia catappa L (Tropical almond) Kernels

  2013cites this paper
• Enhanchement of Lipid Quality of Raw Guinea Corn (Sorghum Bicolor) Grains Through Germination and Steeping

  2013cites this paper
• Domestic Pig Organs as Good Sources of Nutritionally Important Fatty Acids and Low Cholesterol Meat

  2013cites this paper
• Does the trophic habitat influence the biochemical quality of the gonad of Octopus vulgaris? Stable isotopes and lipid class contents as bio-indicators of different life-cycle strategies

  2013cites this paper
• DHA : docosahexaenoic acid EPA : eicosapentaenoic acid FAME : fatty acid methyl esters FFA : free fatty acids MUFA : monounsaturated fatty acids NL : neutral lipids PL : phospholipids POL : polar lipids

  2013cites this paper
• Health implications of the consumption of the dietary lipids in the testes of bulls, bucks and African giant pouch rats

  2013cites this paper
• 흰쥐에서 녹차추출물의 트랜스 지방 소장 흡수 억제 작용

  2012cites this paper
• Cherry Silverberry (Elaeagnus multiflora) Wine Mitigates the Development of Alcoholic Fatty Liver in Rats

  2012cites this paper
• Comparative chemical composition of different muscle zones in angler (Lophius piscatorius)

  2012cites this paper
• ROS production and lipid catabolism in desiccating Shorea robusta seeds during aging.

  2012cites this paper
• 보리수열매주의 알코올성 지방간 형성 억제 효과

  2012cites this paper
• Green Tea Extract Decreases the Lymphatic Absorption of Trans Fat in Rats

  2012cites this paper
• Olive Cake and Barley Malt Rootlets in Hen Diets to Improve Egg Lipids and Fatty Acids

  2012cites this paper
• Nutritional values of the lipid composition of the free-range chicken eggs

  2012cites this paper
• Effects of roasting and cooking on the lipid composition of raw groundnut ( Arachis hypogaea ) seeds : dietary implications

  2012cites this paper
• Lipid Composition of Three Different Types of Land Snails Consumed In Nigeria

  2012influential citation
• Plant Physiology and Biochemistry

  2012cites this paper
• Egg Yolk Cholesterol of Hens Fed Barley Malt Rootlets

  2012cites this paper
• Lipid profiles of the skin, muscle and liver of greater cane rat ( Thryonomys swinderianus ): dietary implications

  2012cites this paper
• Enteral Infusion of Green Tea Extract Selectively Enhances the Biliary Secretion of 14 C-Benzo(a)pyrene in Rats without Affecting Other Biliary Lipids

  2011cites this paper
• Chitosan-reduced gold nanoparticles: a novel carrier for the preparation of spray-dried liposomes for topical delivery

  2011cites this paper
• Olive Cake in Laying Hen Diets for Modification of Yolk Lipids

  2011cites this paper
• RESEARCH OPINIONS IN ANIMAL & VETERINARY SCIENCES RESEARCH OPINIONS IN ANIMAL & VETERINARY SCIENCES RESEARCH OPINIONS IN ANIMAL & VETERINARY SCIENCES RESEARCH OPINIONS IN ANIMAL & VETERINARY SCIENCES

  2011cites this paper
• Diffusion by Computer Simulation of Processes on the with in Parallel Redacted for Privacy

  2010cites this paper
• Cell-Penetrating Peptides: A Comparative Study on Lipid Affinity and Cargo Delivery Properties

  2010cites this paper
• Receptor selectivity of enkephalin analogs carrying artificial address peptides.

  2009cites this paper
• SEASONAL VARIATIONS ON THE BIOCHEMICAL COMPOSITION AND LIPID CLASSES OF THE GONADAL AND STORAGE TISSUES OF CRASSOSTREA GIGAS (THUNBERG, 1794) IN RELATION TO THE GAMETOGENIC CYCLE

  2009cites this paper
• Feeding status-related effects of 17 beta-estradiol on liver 3-hydroxy-3-methylglutaryl CoA reductase.

  2009cites this paper
• Interaction of gramicidin S analogs with lipid bilayer membrane.

  2009cites this paper
• Lipid and mineral distribution in different zones of farmed and wild blackspot seabream (Pagellus bogaraveo)

  2009cites this paper
• Effect of feeding barley radicle as fiber source on lipids in hen eggs.

  2009cites this paper
• Bovine neurophysin lipid complex. Their isolation, characterization and reaggregation.

  2009cites this paper
• PROTECTIVE ROLE OF MELATONIN ON SOME BIOCHEMICAL ASPECTS OF NICOTINE ADMINISTRATION ON BRAIN OVARIECTOMIZED FEMALE RATS

  2008cites this paper
• Isolation and Characterization of the Tubular Organelles Induced by Fumarate Reductase Overproduction in Escherichia culi

  2008cites this paper
• Lipid damage in farmed rainbow trout (Oncorhynchus mykiss) after slaughtering and chilled storage

  2008cites this paper
• Protective effect of taurine and quercetin against renal dysfunction associated with the combined use of gentamycin and diclofenac.

  2008cites this paper
• The roles of lipid and protein in the protection of ram spermatozoa at 5\s=deg\C by egg-yolk lipoprotein

  2008cites this paper
• Nutritional composition and safety of Patagonotothenramsayi, a discard species from Patagonian Shelf

  2007cites this paper
• The role of a mixture of green tea, turmeric and chitosan in the treatment of obesity-related testicular disorders

  2007cites this paper
• Technical note: Stability of lipids of frozen albacore (Thunnus alalunga) during steam cooking

  2007cites this paper
• Oleic Acid-Induced Cholelithiasis in Rabbits

  2007cites this paper
• Distribution of lipids and trace minerals in different muscle sites of farmed and wild turbot (Psetta maxima)

  2007cites this paper
• Lysophosphatidylcholine accumulation in the ischemic canine heart

  2006cites this paper
• Seasonal study of the lipid composition in different tissues of the common octopus (Octopus vulgaris)

  2006cites this paper