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Purification and Initial Characterization of Ara h 7, a Peanut Allergen from the 2S Albumin Protein Family.

D. Apostolović,J. Marsh,J. Baumert,Steve L Taylor,A. Westphal,H. D. de Jongh,Phil Johnson,G. D. de Jong,S. Koppelman

Published 2021 in Journal of Agricultural and Food Chemistry

ABSTRACT

2S albumins are important peanut allergens. Within this protein family, Ara h 2 and Ara h 6 have been described in detail, but Ara h 7 has received little attention. We now describe the first purification of Ara h 7 and its characterization. Two Ara h 7 isoforms were purified from peanuts. Mass spectrometry revealed that both the isoforms have a post-translation cleavage, a hydroxyproline modification near the N-terminus, and four disulfide bonds. The secondary structure of both Ara h 7 isoforms is highly comparable to those of Ara h 2 and Ara h 6. Both Ara h 7 isoforms bind IgE, and Ara h 7 is capable of inhibiting the binding between Ara h 2 and IgE, suggesting at least partially cross-reactive IgE epitopes. Ara h 7 was found in all main market types of peanut, at comparable levels. This suggests that Ara h 7 is a relevant allergen from the peanut 2S albumin protein family.

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