Use of HepG2 cell line for direct or indirect mutagens screening: comparative investigation between comet and micronucleus assays.

No abstract is available for this paper.

• Publication year

  2003

• Venue

  Mutation research

• Publication date

  2003-04-20

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1016/S1383-5718(03)00031-7PMID 12694748
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Uridine uptake inhibition assay: an automated micromethod for the screening of cytotoxicity.

  2002cited by this paper
• Studies on the in vivo and in vitro mutagenicity and the lipid peroxidation of chlorinated surface (drinking) water in rats and metabolically competent human cells.

  2002cited by this paper
• Genetic susceptibility to adverse effects of drugs and environmental toxicants. The role of the CYP family of enzymes.

  2001cited by this paper
• Uridine uptake inhibition as a cytotoxicity test for a human hepatoma cell line (HepG2 cells): comparison with the neutral red assay.

  2001cited by this paper
• Use of the alkaline comet assay for industrial genotoxicity screening: comparative investigation with the micronucleus test.

  2001cited by this paper
• Single cell gel electrophoresis assay: a new technique for human biomonitoring studies.

  2000cited by this paper
• In vitro detection of indirect-acting genotoxins in the comet assay using Hep G2 cells.

  2000cited by this paper
• Evaluation of the single cell gel electrophoresis assay with human hepatoma (Hep G2) cells.

  2000cited by this paper
• Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing

  2000cited by this paper
• Quantitative analysis of constitutive and inducible CYPs mRNA expression in the HepG2 cell line using reverse transcription-competitive PCR.

  2000cited by this paper
• Expression of Cytochromes P-450 2E1, 3A4 and 1A1/1A2 in Growing and Confluent Human HepG2 Hepatoma Cells-Effect of Ethanol.

  1999cited by this paper
• Heterocyclic aromatic amines induce DNA strand breaks and cell transformation.

  1999cited by this paper
• Genotoxic effects of heterocyclic aromatic amines in human derived hepatoma (HepG2) cells.

  1999cited by this paper
• The ability of the Comet assay to discriminate between genotoxins and cytotoxins.

  1998cited by this paper
• Modulatory effects of melatonin on genotoxic response of reference mutagens in the Ames test and the comet assay.

  1998cited by this paper
• Use of metabolically competent human hepatoma cells for the detection of mutagens and antimutagens.

  1998cited by this paper
• The nature and origin of DNA single-strand breaks determined with the comet assay.

  1998cited by this paper
• In vitro mutagenicity and genotoxicity study of a number of short-chain chlorinated hydrocarbons using the micronucleus test and the alkaline single cell gel electrophoresis technique (Comet assay) in human lymphocytes: a structure-activity relationship (QSAR) analysis of the genotoxic and cytotoxic

  1998cited by this paper
• Detection of DNA lesions induced by chemical mutagens using the single-cell gel electrophoresis (comet) assay. 2. Relationship between DNA migration and alkaline condition.

  1997cited by this paper
• Comparative evaluation of the in vitro micronucleus test and the alkaline single cell gel electrophoresis assay for the detection of DNA damaging agents: genotoxic effects of cobalt powder, tungsten carbide and cobalt-tungsten carbide.

  1997cited by this paper
• Detection of aneugenic and clastogenic potential of X-rays, directly and indirectly acting chemicals in human hepatoma (Hep G2) and peripheral blood lymphocytes, using the micronucleus assay and fluorescent in situ hybridization with a DNA centromeric probe.

  1996cited by this paper
• Micronuclei-induction and its correlation to cell survival in HeLa cells treated with different doses of adriamycin.

  1996cited by this paper
• Development and validation of alternative metabolic systems for mutagenicity testing in short-term assays.

  1996cited by this paper
• Genotoxicity assessment of aromatic amines and amides in genetically engineered V79 cells.

  1994cited by this paper
• Response of fibroblast cultures from ataxia‐telangiectasia patients to reactive oxygen species generated during inflammatory reactions

  1994cited by this paper
• Induction of Sister-chromatid Exchanges, Micronuclei and Gene Mutations by Indirectly Acting Promutagens Using Human Hepatoma Cells as an Activation System

  1994cited by this paper
• The single cell gel electrophoresis assay (comet assay): a European review.

  1993cited by this paper
• The cytokinesis-block micronucleus technique: a detailed description of the method and its application to genotoxicity studies in human populations.

  1993cited by this paper
• Analysis of rat lymphocyte activation of benzo[a]pyrene, 2-acetylaminofluorene, and several of their metabolites to mutagenic and DNA-damaging species in vitro.

  1991cited by this paper
• Use of human hepatoma cells for in vitro metabolic activation of chemical mutagens/carcinogens.

  1991cited by this paper
• Heterogeneity in Radiation-Induced DNA Damage and Repair in Tumor and Normal Cells Measured Using the “Comet” Assay

  1990cited by this paper
• Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the "comet" assay.

  1990cited by this paper
• Human hepatocytes in genotoxicity assays.

  1990cited by this paper
• [Biotransformation of dimethylnitrosamine].

  1990cited by this paper
• Sulfotransferase-mediated chlorination of 1-hydroxymethylpyrene to a mutagen capable of penetrating indicator cells.

  1990cited by this paper
• The in vivo micronucleus assay in mammalian bone marrow and peripheral blood. A report of the U.S. Environmental Protection Agency Gene-Tox Program.

  1990cited by this paper
• Cytotoxic and Genotoxic Effects of 10 N-Nitroso Compounds in a Human Hepatoma Cell Line (Hep G2): Comparison with Human Hepatocyte Primary Cultures

  1989cited by this paper
• A simple technique for quantitation of low levels of DNA damage in individual cells.

  1988cited by this paper
• The effectiveness of S9 and microsomal mix on activation of cyclophosphamide to induce genotoxicity in human lymphocytes.

  1987cited by this paper
• N-nitrosamines: bacterial mutagenesis and in vitro metabolism.

  1987cited by this paper
• Drug metabolism by the human hepatoma cell, Hep G2.

  1987cited by this paper
• Measurement of micronuclei in lymphocytes.

  1985cited by this paper
• Genotoxic activity and potency of 135 compounds in the Ames reversion test and in a bacterial DNA-repair test.

  1984cited by this paper
• Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells.

  1984cited by this paper
• Comparison of DNA-repair synthesis, chromosome aberrations and induction of micronuclei in cultured human fibroblasts, Chinese hamster ovary and central mudminnow (Umbra limi) cells exposed to chemical mutagens.

  1984cited by this paper
• The induction of micronuclei as a measure of genotoxicity. A report of the U.S. Environmental Protection Agency Gene-Tox Program.

  1983cited by this paper
• Metabolism of polycyclic aromatic hydrocarbons: etiologic role in carcinogenesis.

  1982cited by this paper
• Enhanced mutagenic activities of N-nitroso compounds in weakly acidic media.

  1980cited by this paper
• Metabolic activation of benzo[a]pyrene by a human hepatoma cell line.

  1980cited by this paper
• Limitations of metabolic activation systems used with in vitro tests for carcinogens.

  1980cited by this paper
• Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen.

  1980cited by this paper
• Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line

  1979cited by this paper
• The production of micronuclei from chromosome aberrations in irradiated cultures of human lymphocytes.

  1976cited by this paper

• Assessment of the Genotoxic Hazard of Estuarine Sediments Using an Integrative Approach With LacZ Plasmid‐Based Transgenic Mice

  2025cites this paper
• Ultrasound-assisted recovery of blackcurrant press cake anthocyanins: Antioxidant and anti-inflammatory properties, bioaccessibility, and application in functional gummies

  2025cites this paper
• Discovery of novel octahydroquinazoline scaffolds endowed with dual inhibition of tubulin polymerization/Eg5 against HCC: Apoptotic and radio-chemotherapeutic studies.

  2024cites this paper
• Heterocyclic phytometabolites formononetin and arbutin prevent in vitro oxidative and alkylation-induced mutagenicity

  2024cites this paper
• Genotoxicity analysis of a flame retardant, aluminum diethylphosphinate.

  2024cites this paper
• Designing sustainable antioxidant and anti-inflammatory ingredients from seaweeds for functional gummies

  2024cites this paper
• Cytogenotoxic evaluations of leaves and stems extracts of Rubus rosifolius in primary metabolically noncompetent cells

  2023cites this paper
• Pharmacokinetic and toxicological prediction of the chemical constituents of the essential oil of the leaves of Croton heliotropiifolius Kunth

  2023cites this paper
• Anti-Trypanosoma cruzi Activity, Mutagenicity, Hepatocytotoxicity and Nitroreductase Enzyme Evaluation of 3-Nitrotriazole, 2-Nitroimidazole and Triazole Derivatives

  2023cites this paper
• In Vitro Safety, Off-Target and Bioavailability Profile of the Antiviral Compound Silvestrol

  2022cites this paper
• p-Toluenediamine hair dyes induce cellular proliferation and genotoxicity

  2022cites this paper
• Hazard Assessment of Benchmark Metal-Based Nanomaterials Through a Set of In Vitro Genotoxicity Assays.

  2022cites this paper
• In vivo mutagenic effects and oxidative stress parameters evaluation of cypermethrin and benzoate of emamectin and their mixtures in female mice

  2022cites this paper
• Analysis of the In Vitro Toxicity of Nanocelluloses in Human Lung Cells as Compared to Multi-Walled Carbon Nanotubes

  2022cites this paper
• Novel Fat Taste Receptor Agonists Curtail Progressive Weight Gain in Obese Male Mice

  2022cites this paper
• Evaluation of DNA-Damaging Effects Induced by Different Tanning Agents Used in the Processing of Natural Leather—Pilot Study on HepG2 Cell Line

  2022cites this paper
• Investigating the impact of long term exposure to chemical agents on the chromosomal radiosensitivity using human lymphoblastoid GM1899A cells

  2021cites this paper
• Micronucleus assays with the human derived liver cell line (Huh6): A promising approach to reduce the use of laboratory animals in genetic toxicology.

  2021cites this paper
• HepGentox: a novel promising HepG2 reportergene-assay for the detection of genotoxic substances in complex mixtures

  2021cites this paper
• Genotoxicity of organic material extracted from particulate matter of alternative fuels

  2021cites this paper
• Cytotoxicity, genotoxicity and efficacy evaluation of the quercus infectoria in HepG2 and WRL68 cell lines

  2021cites this paper
• In VITRO biochemical characterization and genotoxicity assessment of Sapindus saponaria seed extract.

  2021cites this paper
• SpheroidChip: Patterned Agarose Microwell Compartments Harboring HepG2 Spheroids are Compatible with Genotoxicity Testing.

  2020cites this paper
• New insight into the antigenotoxic activity of Gentiana lutea extracts - Protective effect against food borne mutagens.

  2020cites this paper
• Biocompatibility evaluation on HepG2 and HUAEC of pristine and N-doped carbon nanotubes

  2020cites this paper
• β-Carotene and its physiological metabolites: Effects on oxidative status regulation and genotoxicity in in vitro models.

  2020cites this paper
• Genotoxic activities of wastewater after ozonation and activated carbon filtration: Different effects in liver-derived cells and bacterial indicators.

  2020cites this paper
• Following the adverse outcome pathway from micronucleus to cancer using H2B-eGFP transgenic healthy stem cells

  2020cites this paper
• Design of Novel Inhibitors of Human Thymidine Phosphorylase: Synthesis, Enzyme Inhibition, in Vitro Toxicity, and Impact on Human Glioblastoma Cancer.

  2019cites this paper
• Value and limitation of in vitro bioassays to support the application of the threshold of toxicological concern to prioritise unidentified chemicals in food contact materials

  2019cites this paper
• Researcher

  2019cites this paper
• Investigating landfill leachate toxicity in vitro: A review of cell models and endpoints.

  2019cites this paper
• Quantitative comparison of in vitro genotoxicity between metabolically competent HepaRG cells and HepG2 cells using the high-throughput high-content CometChip assay

  2019cites this paper
• Assessment of the predictive capacity of the optimized in vitro comet assay using HepG2 cells.

  2018cites this paper
• New hydrazides derivatives of isoniazid against Mycobacterium tuberculosis: Higher potency and lower hepatocytotoxicity.

  2018cites this paper
• Genotoxic effects of food contact recycled paperboard extracts on two human hepatic cell lines

  2018cites this paper
• Design, synthesis and pharmacological evaluation of N-benzyl-piperidinyl-aryl-acylhydrazone derivatives as donepezil hybrids: Discovery of novel multi-target anti-alzheimer prototype drug candidates.

  2018cites this paper
• The characterization of γH2AX and p53 as biomarkers of genotoxic stress in a rainbow trout (Oncorhynchus mykiss) brain cell line.

  2018cites this paper
• Psidium guajava Leaf Extracts and Their Quercetin Protect HepG2 Cell Lines Against CCL4 Induced Cytotoxicity

  2018cites this paper
• Toxicogenomics of the flame retardant tris (2-butoxyethyl) phosphate in HepG2 cells using RNA-seq.

  2018cites this paper
• Caryophyllane sesquiterpenes inhibit DNA-damage by tobacco smoke in bacterial and mammalian cells.

  2018cites this paper
• Genotoxicity and Cytotoxicity Evaluation Applied to Environmental Health, Research of Polycyclic Aromatic Hydrocarbons

  2018cites this paper
• Knock-down of AHCY and depletion of adenosine induces DNA damage and cell cycle arrest

  2018cites this paper
• Genotoxicity and cytotoxicity of three microcystin‐LR containing cyanobacterial samples from Antioquia, Colombia

  2018cites this paper
• Protective effects of Bidens pilosa on hepatoxicity and nephrotoxicity induced by carbon tetrachloride in rats

  2018cites this paper
• Evaluating the genotoxicity of cellulose nanofibrils in a co-culture of human lung epithelial cells and monocyte-derived macrophages.

  2018cites this paper
• Unravelling genotoxic effects of pesticides : in vitro and ex vivo assays as screening tools

  2018cites this paper
• Estudos in vitro da genotoxicidade e citotoxicidade em células hepáticas da formação de 2-alcilciclobutanonas resultantes da irradiação de alimentos que contenham gordura

  2017cites this paper
• Cytotoxic and genotoxic potential of geraniol in peripheral blood mononuclear cells and human hepatoma cell line ( HepG 2 )

  2017cites this paper
• Inhibitory activity of pentacyano(isoniazid)ferrate(II), IQG-607, against promastigotes and amastigotes forms of Leishmania braziliensis

  2017cites this paper
• The Possible Protective Effect of Antioxidant Alpha-Lipoic Acid on the Postnatal Developing Liver of Albino Rats Treated with Doxorubicin (Histological and Cytogenetic Study)

  2017cites this paper
• Use of HuH6 and other human-derived hepatoma lines for the detection of genotoxins: a new hope for laboratory animals?

  2017cites this paper
• Chemical characterization and in vitro cyto- and genotoxicity of ‘legal high’ products containing Kratom (Mitragyna speciosa)

  2016cites this paper
• Assessment of oxidative stress responses and the cytotoxic and genotoxic potential of the herbicide tembotrione in HepG2 cells.

  2016cites this paper
• Spatio-temporal distribution, source, and genotoxic potential of polycyclic aromatic hydrocarbons in estuarine and riverine sediments from southern India.

  2016cites this paper
• Cytotoxicity of mequindox and its metabolites in HepG2 cells in vitro and murine hepatocytes in vivo.

  2016cites this paper
• Mutation Research/Genetic Toxicology and Environmental Mutagenesis

  2016cites this paper
• Evaluation of the cytotoxic and genotoxic effects of benchmark multi-walled carbon nanotubes in relation to their physicochemical properties.

  2016cites this paper
• The consequences of physical post-treatments (microwave and electron-beam) on food/packaging interactions: A physicochemical and toxicological approach.

  2016cites this paper
• Cytotoxic, genotoxic and biochemical markers of insecticide toxicity evaluated in human peripheral blood lymphocytes and an HepG2 cell line.

  2016cites this paper
• Comparative genotoxicity investigation using comet and gammaH2AX assays for screening of genotoxicants in HepG2 human hepatoma cells

  2016cites this paper
• In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: a promising class of antituberculosis agents.

  2016cites this paper
• The clastogenicity of 4 NQO is cell-type dependent and linked to cytotoxicity , length of exposure and p 53 proficiency

  2016cites this paper
• Exploiting microRNA and mRNA profiles generated in vitro from carcinogen-exposed primary mouse hepatocytes for predicting in vivo genotoxicity and carcinogenicity.

  2016cites this paper
• Cytotoxic and genotoxic effects of two hair dyes used in the formulation of black color.

  2015cites this paper
• Analysis of the genotoxic potential of low concentrations of Malathion on the Allium cepa cells and rat hepatoma tissue culture.

  2015cites this paper
• Evaluating microRNA profiles reveals discriminative responses following genotoxic or non-genotoxic carcinogen exposure in primary mouse hepatocytes.

  2015cites this paper
• Phenolic Profile, Antioxidant Activities and Genotoxic Evaluations of Calendula officinalis

  2015cites this paper
• Micronucleus formation by single and mixed heavy metals/loids and PAH compounds in HepG2 cells.

  2015cites this paper
• Clinical Studies of Traditional Therapeutic Herbs before Marketing: Current Status and Future Challenges

  2015cites this paper
• The clastogenicity of 4NQO is cell-type dependent and linked to cytotoxicity, length of exposure and p53 proficiency

  2015cites this paper
• Cytotoxicity and genotoxicity of orthodontic bands with or without silver soldered joints.

  2014cites this paper
• A comparative toxicity evaluation of Escherichia coli-targeted ssDNA and chlorine in HepG2 cells.

  2014cites this paper
• Genotoxicity of Microcystin-LR in In Vitro and In Vivo Experimental Models

  2014cites this paper
• Universidade Federal da Paraíba Centro de Ciências da Saúde Programa de Pós-graduação em Produtos Naturais e Sintéticos Bioativos Investigação dos efeitos antimicrobiano, citotóxico e genotóxico do óleo essencial das folhas de Croton tricolor Klotsch ex Baill (Euphorbiaceae)

  2014cites this paper
• Anti-genotoxicity and anti-mutagenicity of Apis mellifera venom.

  2014cites this paper
• Performance of comet and micronucleus assays in metabolic competent HepaRG cells to predict in vivo genotoxicity.

  2014cites this paper
• Soil quality in the Lomellina area using in vitro models and ecotoxicological assays.

  2014cites this paper
• Determining oxidative and non-oxidative genotoxic effects driven by estuarine sediment contaminants on a human hepatoma cell line.

  2014cites this paper
• Assessment of genotoxicity and cytotoxicity of standardized aqueous extract from leaves of Erythroxylum cuneatum in human HepG2 and WRL68 cells line.

  2013cites this paper
• Chemical-based risk assessment and in vitro models of human health effects induced by organic pollutants in soils from the Olona Valley.

  2013cites this paper
• PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS BIOLÓGICAS (BIOLOGIA CELULAR E MOLECULAR) AVALIAÇÃO DA TOXICIDADE E CARACTERIZAÇÃO DE HIDROCARBONETOS PRESENTES EM ÁGUAS DE RIOS IMPACTADOS POR EFLUENTE DE INDÚSTRIA PETROQUÍMICA

  2013cites this paper
• Comparative study of cytotoxic and genotoxic effects induced by herbicide S-metolachlor and its commercial formulation Twin Pack Gold® in human hepatoma (HepG2) cells.

  2013cites this paper
• Global Risk-Based Management of Chemical Additives II: Risk-Based Assessment and Management Strategies

  2013influential citation
• Estudo do potencial genotóxico da Gutiferona A em diferentes células de camundongos in vitro

  2013cites this paper
• Genotoxicity and Carcinogenicity: Regulatory and Novel Test Methods

  2013cites this paper
• Natural Antioxidants and Biocides from Wild Medicinal Plants

  2013cites this paper
• Antigenotoxic effect, composition and antioxidant activity of Dendrobium speciosum.

  2013cites this paper
• Toxicological Characterization of Waste-Related Products Using Alternative Methods: Three Case Studies

  2012cites this paper
• An adaptation of the human HepaRG cells to the in vitro micronucleus assay.

  2012cites this paper
• The ribosome-inactivating protein gelonin and parts thereof to be employed for a potential treatment of cancer

  2012cites this paper
• Caracterização do Potencial Citotóxico e Mecanismos de Acção de um Complexo de Platina de Configuração trans em Células Animais

  2012cites this paper
• Protective Effects of Green and White Tea Against Benzo(a)pyrene Induced Oxidative Stress and DNA Damage in Murine Model

  2012cites this paper
• Comparison of DNA damage in human-derived hepatoma line (HepG2) exposed to the fifteen drinking water disinfection byproducts using the single cell gel electrophoresis assay.

  2012cites this paper
• The ribosome-inactivating protein gelonin and parts thereof to be employed for a potential treatment of cancer

  2012cites this paper
• Genotoxic evaluation of the non-halogenated disinfection by-products nitrosodimethylamine and nitrosodiethylamine.

  2011cites this paper
• Low-dose effects and biphasic effect profiles: is trenbolone a genotoxicant?

  2011cites this paper
• Impacts of two perfluorinated compounds (PFOS and PFOA) on human hepatoma cells: cytotoxicity but no genotoxicity?

  2011cites this paper
• A combined approach to investigate the toxicity of an industrial landfill's leachate: chemical analyses, risk assessment and in vitro assays.

  2011cites this paper
• Combined effects of curcumin and piperine in ameliorating benzo(a)pyrene induced DNA damage.

  2011cites this paper