Three dimensional tracking of fluorescent microparticles using a photon-limited double-helix response system.

We demonstrate three-dimensional tracking of fluorescent microparticles, with a computational optical system whose point spread function (PSF) has been engineered to have two twisting lobes along the optical axis, generating a three-dimensional (3D) double-helix (DH) PSF. An information theoretical comparison in photon limited systems shows that the DH-PSF delivers higher Fisher information for 3D localization than the standard PSF. Hence, DH-PSF systems provide better position estimation accuracy. Experiments demonstrate average position estimation accuracies under 14nm and 37nm in the transverse and axial dimensions respectively. The system determines the 3D position of multiple particles with a single image and tracks them over time while providing their velocities.

• Publication year

  2008

• Venue

  Optics Express

• Publication date

  2008-12-22

• Fields of study

  Medicine, Physics, Engineering

• Identifiers
  DOI 10.1364/OE.16.022048PMID 19104639
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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