LncRNA PSMA3‐AS1 promotes cell proliferation, migration, and invasion in ovarian cancer by activating the PI3K/Akt pathway via the miR‐378a‐3p/GALNT3 axis

The crucial roles of the long noncoding RNAs (lncRNAs) in the development of ovarian cancer (OC) have been extensively studied. According to the prediction result from the Kaplan–Meier Plotter database, high expression of lncRNA proteasome subunit α type‐3 antisense RNA1 (PSMA3‐AS1) is associated with the poor prognosis in patients with OC. Thus, the study aimed to investigate the role of lncRNA PSMA3‐AS1 in OC. Reverse transcription quantitative polymerase chain reaction analysis revealed that PSMA3‐AS1 expression was significantly upregulated in OC cells and tissues. PSMA3‐AS1 silencing inhibited OC cell proliferation, migration, and invasion, as shown by results of cell counting kit‐8, colony formation, wound healing, and Transwell assays, respectively. Additionally, PSMA3‐AS1 deficiency suppressed tumor growth in vivo. Mechanistically, luciferase reporter and RNA pulldown assays implied that PSMA3‐AS1 served as a competing endogenous RNA for miR‐378a‐3p to upregulate the expression of polypeptide N‐acetylgalactosaminyltransferase 3 (GALNT3). GALNT3 was a target gene of miR‐378a‐3p in OC. Moreover, PSMA3‐AS1 activated the PI3K/Akt pathway by upregulating GALNT3 expression. Overall, PSMA3‐AS1 promotes OC cell proliferation, migration, invasion, and xenograft tumor growth by activating the PI3K/Akt pathway via the miR‐378a‐3p/GALNT3 axis.

• Publication year

  2021

• Venue

  Environmental Toxicology

• Publication date

  2021-09-14

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1002/tox.23370PMID 34520102
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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