Structural basis of proline-specific exopeptidase activity as observed in human dipeptidyl peptidase-IV.

No abstract is available for this paper.

• Publication year

  2003

• Venue

  Structure

• Publication date

  2003-08-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/S0969-2126(03)00160-6PMID 12906826
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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• Protein folds propelled by diversity.

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  2000cited by this paper
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• Dipeptidyl Peptidase IV (DPIV/CD26) Degradation of Glucagon

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• A guardian angel: the involvement of dipeptidyl peptidase IV in psychoneuroendocrine function, nutrition and immune defence.

  2000cited by this paper
• A guardian angel.

  2000cited by this paper
• Beta propellers: structural rigidity and functional diversity.

  1999cited by this paper
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  1998influential reference
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• Proline specific peptidases.

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• Adenosine deaminase affects ligand‐induced signalling by interacting with cell surface adenosine receptors

  1996cited by this paper
• Phosphoribosyl anthranilate isomerase from Thermotoga maritima is an extremely stable and active homodimer

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  1995cited by this paper
• The CCP4 suite: programs for protein crystallography.

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• Dipeptidyl-peptidase IV from rat liver.

  1994cited by this paper
• Expression and functional role of dipeptidyl peptidase IV (CD26) on human natural killer cells.

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• Binding of adenosine deaminase to the lymphocyte surface via CD26

  1994cited by this paper
• Cloning and functional expression of the T cell activation antigen CD26.

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• Light scattering and the absolute characterization of macromolecules

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• The α/β hydrolase fold

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• Identification of the active site residues in dipeptidyl peptidase IV by affinity labeling and site-directed mutagenesis.

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  1978cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

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• Tissue sulfhydryl groups.

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