Non-specific binding of protein-stabilized gold sols as a source of error in immunocytochemistry.

No abstract is available for this paper.

• Publication year

  1986

• Venue

  European Journal of Cell Biology

• Publication date

  1986-08-01

• Fields of study

  Biology, Medicine

• Identifiers
  PMID 3530765
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Analysis of colloidal gold methods for labelling proteins

  2004cited by this paper
• Labelling of colloidal gold with protein

  2004cited by this paper
• Sensitive colloidal metal (gold or silver) staining of protein blots on nitrocellulose membranes.

  1985cited by this paper
• Filaggrin, the matrix protein of keratin.

  1985cited by this paper
• Intratumoral phenotypic diversity of cloned human lung tumor cell lines and consequences for analyses with monoclonal antibodies

  1984cited by this paper
• "Golden blot"--detection of polyclonal and monoclonal antibodies bound to antigens on nitrocellulose by protein A-gold complexes.

  1984cited by this paper
• Sensitive visualization of antigen-antibody reactions in dot and blot immune overlay assays with immunogold and immunogold/silver staining.

  1984cited by this paper
• An evaluation of the conditions necessary for optimal protein A-gold labelling of capsular antigen in ultrathin methacrylate sections of the bacteriumPasteurella haemolytica

  1984cited by this paper
• Immunogold for detection of antigen on nitrocellulose paper.

  1984cited by this paper
• Ultrastructural localization of antigenic sites on osmium-fixed tissues applying the protein A-gold technique.

  1983cited by this paper
• Visualization of microtubules in interphase and mitotic plant cells of Haemanthus endosperm with the immuno-gold staining method.

  1982cited by this paper
• Sizing of protein A-colloidal gold probes for immunoelectron microscopy

  1981cited by this paper
• Cell surface distribution of fibronectin in cultures of fibroblasts and bladder derived epithelium: SEM‐immunogold localization compared to immunoperoxidase and immunofluorescence

  1981cited by this paper
• Cell surface labelling with gold colloid particulates: the use of avidin and staphylococcal protein A‐coated gold in conjunction with biotin and fc‐bearing ligands

  1981cited by this paper
• Colloidal gold markers and probes for routine application in microscopy

  1981cited by this paper
• High resolution light and electron microscopic localization of tubulin with the IGS (immuno gold staining) method.

  1981cited by this paper
• Use of colloidal gold particles in double-labeling immunoelectron microscopy of ultrathin frozen tissue sections

  1981cited by this paper
• Quantitative immunocytochemical localization of pancreatic secretory proteins in subcellular compartments of the rat acinar cell.

  1980cited by this paper
• Selective binding, uptake, and retrograde transport of tetanus toxin by nerve terminals in the rat iris. An electron microscope study using colloidal gold as a tracer

  1978cited by this paper
• Use of staphylococcal protein A as an immunological reagent.

  1978cited by this paper
• Simultaneous localization of an hepatic binding protein specific for galactose and of galactose-containing receptors on rat hepatocytes.

  1978cited by this paper
• Adsorption of horseradish peroxidase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concanavalin A, wheat germ agglutinin and goat anti-human immunoglobulin G on cell surfaces at the electron microscopic level: a new method, theory and application.

  1977cited by this paper
• The origin of citrulline-containing proteins in the hair follicle and the chemical nature of trichohyalin, an intracellular precursor.

  1977cited by this paper
• Colloidal gold, a useful marker for transmission and scanning electron microscopy.

  1977cited by this paper
• Sulphure in epidermal keratohyalin granules: a quantitative assay by x-ray microanalysis.

  1976cited by this paper

• Degradation of IL-4Ralpha by Immunoproteasome: implication in airway type 2 inflammation and hyperresponsiveness

  2025cites this paper
• A specific technique of immunolabelling of urinary small extracellular vesicle biomarkers for the diagnostic of renal cancer.

  2023cites this paper
• Gold nanomaterials and their potential use as cryo-electron tomography labels.

  2022cites this paper
• Protein A–Gold: Nonspecific Binding and Cross-Contamination

  2012cites this paper
• Function of S-100 Protein with Secretion of Granular Convoluted Tubule in Rat Submandibular Gland

  2011cites this paper
• Site-specific biomolecule labeling with gold clusters.

  2010cites this paper
• Morphological features of established cultures of human squamous lung carcinoma cells and the cellular distribution of tumor-specific glycoproteins.

  2009cites this paper
• Element-specific microtomographic imaging of Drosophila brain stained with high-Z probes.

  2008cites this paper
• Synaptic contacts of vesicular glutamate transporter 2 fibres on chemically identified neurons of the hypothalamic suprachiasmatic nucleus of the rat

  2008cites this paper
• Vesicular glutamate transporter 2 protein and mRNA containing neurons in the hypothalamic suprachiasmatic nucleus of the rat.

  2007cites this paper
• Targeted destruction of murine macrophage cells with bioconjugated gold nanorods

  2007cites this paper
• Metallographic in situ hybridization.

  2007cites this paper
• Herpes simplex virus type 1 morphogenesis and virus‐cell interactions: significance of cytoskeleton and methodological aspects

  2006cites this paper
• Glutamatergic innervation of growth hormone-releasing hormone-containing neurons in the hypothalamic arcuate nucleus and somatostatin-containing neurons in the anterior periventricular nucleus of the rat.

  2006cites this paper
• Immunogold probes in light microscopy.

  2005cites this paper
• Prevention of non-specific interactions of gold-labeled reagents on tissue sections

  2004cites this paper
• Light microscopical detection of leukocyte cell surface antigens with a one-nanometer gold probe

  2004cites this paper
• Histochemical localization of oxidized glutathione-catalysing enzymes in human term placenta

  2004cites this paper
• Orchestin, a calcium-binding phosphoprotein, is a matrix component of two successive transitory calcified biomineralizations cyclically elaborated by a terrestrial crustacean.

  2004cites this paper
• Gold-conjugated arabinogalactan-protein and other lectins as ultrastructural probes for the wheat/stem rust complex

  2004cites this paper
• Reduction of background labeling in colloidal gold-enzyme reactions

  2004cites this paper
• Evidence for vesicular glutamate transporter synapses onto gonadotropin‐releasing hormone and other neurons in the rat medial preoptic area

  2003cites this paper
• Elimination of artifacts on native western blots arising from endogenous lectin activity.

  2002cites this paper
• The Reovirus Mutant tsA279 L2 Gene Is Associated with Generation of a Spikeless Core Particle: Implications for Capsid Assembly

  1999cites this paper
• Chapter 16 – IMMUNOCYTOCHEMISTRY

  1999cites this paper
• A Covalently Linked 10 Nm Gold Immunoprobe

  1999cites this paper
• Giant platinum clusters: 2-nm covalent metal cluster labels.

  1999cites this paper
• Pioloform F Instead of Formvar as a Support Film for TEM

  1998cites this paper
• Immunogold probes in light microscopy.

  1998cites this paper
• Preparation, use, and enlargement of ultrasmall gold particles in immunoelectron microscopy

  1998cites this paper
• Clathrin-mediated endocytosis of high density lipoprotein3 in human intestinal Caco-2 cells. A post-embedding immunocytochemical study.

  1997cites this paper
• Improved immuno double labelling for cell and structural biology.

  1996cites this paper
• Cholesteryl ester absorption by small intestinal brush border membrane is protein-mediated.

  1995cites this paper
• A novel rat mesangial matrix protein, MMP-50/100, involved in mesangial glomerulopathies.

  1995cites this paper
• Confocal epipolarization microscopy of gold probes in plant cells and protoplasts.

  1995cites this paper
• Characterization of Lipid Exchange Proteins Isolated from Small Intestinal Brush Border Membrane (*)

  1995cites this paper
• Colloidal gold post-embedding immunocytochemistry.

  1995cites this paper
• Transmission electron microscopy and scanning probe microscopy.

  1994cites this paper
• Visualization of elicitor-binding loci at the plant cell surface

  1994influential citation
• EVALUATION OF DOUBLE IMMUNOLABELING METHODS FOR EPITOPE MAPPING ON ISOLATED Na, K-ATPase MEMBRANES

  1994cites this paper
• Postembedding Light and Electron Microscopic Immunocytochemistry in Pineal Photoneuroendocrinology

  1994cites this paper
• Histological evaluation of the zonation of colloidal gold uptake by the rat liver.

  1993cites this paper
• Labelling Reactions for Immunocytochemistry

  1993cites this paper
• Suitability of different protein A-gold markers for immunogold-silver staining in paraffin sections.

  1993cites this paper
• Colloidal gold and its application in cell biology.

  1992cites this paper
• Microwave-aided binding of gold-protein-ligand (GPL) complexes. Light microscopic observations in the rat brain.

  1992cites this paper
• 4 – Polar or Apolar Lowicryl Resin for Immunolabeling?

  1991cites this paper
• Resinless section immunogold electron microscopy of karyo-cytoskeletal frameworks of eukaryotic cells cultured in vitro. Absence of a salt-stable nuclear matrix from mouse plasmacytoma MPC-11 cells.

  1991cites this paper
• Molecular differentiation in pea powdery-mildew haustoria

  1991cites this paper
• Intracellular localization of glycosyl-phosphatidylinositol-anchored CD67 and FcRIII (CD16) in affected neutrophil granulocytes of patients with paroxysmal nocturnal hemoglobinuria.

  1991cites this paper
• Immunogold labelling in combination with cryoultramicrotomy, freeze‐etching, and label‐fracture

  1991cites this paper
• Lectinocytochemical specificity in human eosinophils and neutrophils: a reexamination.

  1991cites this paper
• Glomerular epithelial cell endocytosis of immune deposits in human lupus nephritis.

  1991cites this paper
• Immunocytochemical localization of nuclear antigens in the unicellular green alga,Chlamydomonas reinhardtii, processed by cryofixation and freeze-substitution

  1991cites this paper
• On the origin of the FcRIII (CD16)-containing vesicle population in human neutrophil granulocytes.

  1991cites this paper
• 10 – Location and Identification of Colloidal Gold Particles with an Energy-Dispersive Analyzer

  1991cites this paper
• Glycinergic contacts in the outer plexiform layer of the Xenopus laevis retina characterized by antibodies to glycine, GABA, and glycine receptors

  1990cites this paper
• Localization of the low-Mr subunit of cytochrome b558 in human blood phagocytes by immunoelectron microscopy.

  1990cites this paper
• Immuno- and affinity probes for electron microscopy: a review of labeling and preparation techniques.

  1990cites this paper
• Prevention of nonspecific binding of avidin.

  1990cites this paper
• Non-specific binding of immunoglobulins and protein A-Gold complex with cytoplasmic granules of human mast cells.

  1990cites this paper
• Intracellular localization and de novo synthesis of FcRIII in human neutrophil granulocytes.

  1990cites this paper
• Intracellular localization and de novo synthesis of FcRIII in human neutrophil granulocytes

  1990cites this paper
• Immunocytochemistry of the cell nucleus.

  1990cites this paper
• Quantitative studies of keratin expression with the post-embedding immunogold labeling method.

  1989cites this paper
• An immunogold-silver staining method for detection of cell surface antigens in cell smears.

  1989cites this paper
• 11 – Strategies in Colloidal Gold Labeling of Cell Surfaces and Cytoskeletal Elements of Cultured Cells

  1989cites this paper
• Factors affecting efficiency of colloidal gold staining: pH-dependent stability of protein-gold conjugates.

  1989cites this paper
• 18 – Factors Affecting the Staining with Colloidal Gold

  1989cites this paper
• Immunoelectron microscopy of rhodopsin and vitamin A in the pineal organ and lateral eye of the lamprey.

  1989cites this paper
• Ultrastructural localization of lectin-binding sites and laminin-like immunoreactivity in glial cells and neurites growing out from explant cultures of the central nervous system of embryonic locusts

  1989cites this paper
• Immunolabeling with the protein A-gold technique: an overview.

  1989cites this paper
• Microscopical, Biochemical, and Immunological Studies of the Immune Defense System of the Horseshoe Crab, Limulus polyphemus.

  1989cites this paper
• Coated Pits and Vesicles Transfer Plasma Components to Platelet Granules

  1989cites this paper
• Immunoelectron microscopy of lipopolysaccharide in Chlamydia trachomatis

  1989cites this paper
• Removal of background label in immunocytochemistry with the apolar Lowicryls by using washed protein A-gold-precoupled antibodies in a one-step procedure.

  1989cites this paper
• A new approach to study mucoadhesion: colloidal gold staining

  1989cites this paper
• 9 – Silver-Enhanced Colloidal Gold Method

  1989cites this paper
• 15 – Colloidal Gold for Microbiological Immunocytochemistry

  1989cites this paper
• Light and electron microscopic demonstration of extracellular immunoglobulin deposition in renal tissue.

  1989cites this paper
• Immunocytochemical Localization of the Vacuolar H-ATPase in Maize Root Tip Cells.

  1989cites this paper
• Postembedding labeling on Lowicryl K4M tissue sections: detection and modification of cellular components.

  1989cites this paper
• Reevaluation of fibronectin-collagen interactions in tissues: an immunocytochemical and immunochemical study.

  1988cites this paper
• Immunogold quantitation of laminin, type IV collagen, and heparan sulfate proteoglycan in a variety of basement membranes.

  1988cites this paper
• Location and identification of colloidal gold particles on the cell surface with a scanning electron microscope and energy dispersive analyzer.

  1988cites this paper
• Bioapplication of colloidal gold in microbiological immunocytochemistry.

  1988cites this paper
• Selective binding of colloidal gold-protein conjugates to secretory granules of granular skin glands and myelin sheaths in the skin of Xenopus laevis.

  1988cites this paper
• Surface membrane clearing of receptor-ligand complexes in human blood platelets.

  1987cites this paper
• Pitfalls of immunogold labeling: analysis by light microscopy, transmission electron microscopy, and photoelectron microscopy.

  1987cites this paper