Colloidal gold post-embedding immunocytochemistry.

No abstract is available for this paper.

• Publication year

  1995

• Venue

  Progress in Histochemistry and Cytochemistry

• Publication date

  1995-08-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0079-6336(11)80027-6PMID 7480783
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The use of the avidin-biotin complex as a tool in molecular biology.

  2006cited by this paper
• Double labelling of cell surface antigens with colloidal gold markers

  2005cited by this paper
• Ultrastructural localization of actin in muscle, epithelial and secretory cells by applying the protein A-gold immunocytochemical technique

  2005cited by this paper
• Concentration of amylase along its secretory pathway in the pancreatic acinar cell as revealed by high resolution immunocytochemistry

  2005cited by this paper
• The potential of the immunogold-silver staining method for paraffin sections

  2004cited by this paper
• Simultaneous demonstration of multiple antigens by indirect immunofluorescence or immunogold staining

  2004cited by this paper
• Potency of microwave irradiation during fixation for electron microscopy

  2004cited by this paper
• Prevention of non-specific interactions of gold-labeled reagents on tissue sections

  2004cited by this paper
• New sensitive light microscopical detection of colloidal gold on ultrathin sections by reflection contrast microscopy

  2004cited by this paper
• Early functional and morphologic vascular renal consequences of the diabetic state

  2004cited by this paper
• “Thiocyanate gold”: small (2–3 nm) colloidal gold for affinity cytochemical labeling in electron microscopy

  2004cited by this paper
• Controlled growth of colloidal gold particles and implications for labelling efficiency

  2004cited by this paper
• The use of avidin-gold complex for light microscopic localization of lectin receptors

  2004cited by this paper
• Resin embedment of organs and postembedment localization of antigens by immunoperoxidase methods

  2004cited by this paper
• Ultrastructural detection of lectin receptors by cytochemical affinity reaction using mannan-iron complex

  2004cited by this paper
• Simultaneous triple-immunogold staining of virus and host cell antigens with monoclonal antibodies of virus and host cell antigens in ultrathin cryosections

  2004cited by this paper
• Ultrastructural localization of extracellular immunoglobulins in immune-complex-mediated glomerulonephritis

  2004cited by this paper
• Simplified purification and testing of colloidal gold probes

  2004cited by this paper
• An Optimized Protocol for In Situ Hybridization Using PCR-Generated 33P-Labeled Riboprobes

  1996cited by this paper
• Immunocytochemical investigation of the in vivo endocytosis by renal tubular epithelial cells

  1995cited by this paper
• Presence of tumor necrosis factor alpha and interleukin-6 in renal mesangial cells of lupus nephritis patients.

  1995cited by this paper
• Possibilities of false immunocytochemical results generated by the use of monoclonal antibodies: the example of the anti-proinsulin antibody.

  1995cited by this paper
• Biochemical and morpho-cytochemical evidence for the intestinal absorption of insulin in control and diabetic rats. Comparison between the effectiveness of duodenal and colon mucosa

  1994cited by this paper
• Molecular chaperones in pancreatic tissue: the presence of cpn10, cpn60 and hsp70 in distinct compartments along the secretory pathway of the acinar cells.

  1994cited by this paper
• A negative contrast stain for ultra‐thin frozen sections

  1994cited by this paper
• A comparison of heating modes in rapid fixation techniques for electron microscopy.

  1994cited by this paper
• Filipin-sensitive caveolae-mediated transport in endothelium: reduced transcytosis, scavenger endocytosis, and capillary permeability of select macromolecules

  1994cited by this paper
• Cryofixation combined with physical dehydration for quantitative immunoelectron cytochemistry.

  1994cited by this paper
• Cryofixation, cryosubstitution, cryoembedding for ultrastructural, immunocytochemical and microanalytical studies.

  1994cited by this paper
• Compartmentalization of secretory proteins in pancreatic zymogen granules as revealed by immunolabeling on cryo‐fixed and molecular distillation processed tissue *

  1994cited by this paper
• Preservation and contrast without osmication or section staining

  1994cited by this paper
• Methods of microwave fixation for microscopy. A review of research and clinical applications: 1970-1992.

  1994cited by this paper
• Improved method for post-embedding cytochemistry using reduced osmium and LR white resin.

  1994cited by this paper
• Cryofixation and cryosubstitution: a useful alternative in the analyses of cellular fine structure.

  1993cited by this paper
• Poly-L-lysine-gold probe for the detection of anionic sites in normal glomeruli and in idiopathic and experimentally-induced nephrosis. A comparative ultrastructural study.

  1993cited by this paper
• Immunodetection of low concentrations of ovalbumin in cryoprocessed quail oviduct cells by semi‐automatic quantitative analysis

  1993cited by this paper
• Fundamental principles of in situ hybridization.

  1993cited by this paper
• Ultrastructural localization of DNA in immune deposits of human lupus nephritis.

  1993cited by this paper
• Scanning tunneling microscopy of colloidal gold beads

  1993cited by this paper
• Freeze substitution after fast‐freeze fixation in preparation for immunocytochemistry

  1993cited by this paper
• A new polychrome stain and simultaneous methods of histological, histochemical and immunohistochemical stainings performed on semithin sections of Bioacryl-embedded human tissues

  1993cited by this paper
• Preservation of antigenic reactivity after cryofixation, cryosubstitution, and cryoembedding in Lowicryl HM23: Application to alcohol dehydrogenase in Drosophila fat body

  1993cited by this paper
• Pathway of insulin in pancreatic tissue on its release by the B-cell.

  1993cited by this paper
• Impact of freeze substitution on biological electron microscopy

  1993cited by this paper
• Hapten‐tagged plasma proteins as immunocytochemical probes for the study of vascular permeability

  1992cited by this paper
• Ultrastructural localization of endogenous albumin in human aortic tissue by protein A-gold immunocytochemistry.

  1992cited by this paper
• A new acrylic resin formulation: a useful tool for histological, ultrastructural, and immunocytochemical investigations.

  1992cited by this paper
• Freeze Substitution and Low-Temperature Embedding

  1992cited by this paper
• Improved accuracy in diagnostic immunohistochemistry, lectin histochemistry and in situ hybridization using a gold-labeled horseradish peroxidase antibody and silver intensification.

  1992cited by this paper
• Transendothelial transport of serum albumin: a quantitative immunocytochemical study

  1992cited by this paper
• The use of osmicated tissues for Lowicryl K4M embedding.

  1992cited by this paper
• Colloidal gold and its application in cell biology.

  1992cited by this paper
• Immunocytochemical localization of surfactant protein D (SP-D) in type II cells, Clara cells, and alveolar macrophages of rat lung.

  1992cited by this paper
• Immunocytochemical study of glomerular permeability to anionic, neutral and cationic albumins.

  1992cited by this paper
• Recent advances in the polymerase chain reaction

  1991cited by this paper
• The potential of cryofixation and freeze substitution: observations and theoretical considerations

  1991cited by this paper
• Surfactant protein A is localized at the corners of the pulmonary tubular myelin lattice.

  1991cited by this paper
• Immunocytochemical localization of apolipoprotein A‐I using polyclonal antibodies raised against the formaldehyde‐modified antigen

  1991cited by this paper
• Cryofixation and ultra‐low‐temperature freeze‐drying as a preparative technique for TEM

  1991cited by this paper
• 5 – Yield of Immunolabel Compared to Resin Sections and Thawed Cryosections

  1991cited by this paper
• Protein AG-gold complex: an alternative probe in immunocytochemistry.

  1991cited by this paper
• 16 – Colloidal Gold Labeling for Diagnostic Pathology

  1991cited by this paper
• Commercial preparations of colloidal gold-antibody complexes frequently contain free active antibody.

  1991cited by this paper
• Cryofixation, cryosubstitution, cryo‐embedding for visualizing of nuclear ultrastructure and for immunodetection HeLa cells

  1991cited by this paper
• 17 – Immunogold – Silver Staining Using Microwave Irradiation

  1991cited by this paper
• Post-embedding immunoelectron microscopy of tissue processed by rapid freezing and freeze-substitution without chemical fixatives.

  1991cited by this paper
• Cell walls of normal and lysozyme-damaged blastoconidia of Candida albicans: localization of surface factor 4 antigen and vicinal-glycol staining

  1991cited by this paper
• Microwave energy fixation of plant tissue: an alternative approach that provides excellent preservation of ultrastructure and antigenicity.

  1991cited by this paper
• 18 – A Correlative Approach to Colloidal Gold Labeling with Video-Enhanced Light Microscopy, Low-Voltage Scanning Electron Microscopy, and High-Voltage Electron Microscopy

  1991cited by this paper
• Advantages of fast-freeze fixation followed by freeze-substitution for the preservation of cell integrity.

  1991cited by this paper
• 12 – Preparation and Application of Insulin – Gold Complex

  1991cited by this paper
• Immunoelectron microscopy of tissues processed by rapid freezing and freeze-substitution fixation without chemical fixatives: application to catalase in rat liver hepatocytes.

  1990cited by this paper
• Double and triple immunocytochemical labelling at the light microscope level in histopathology

  1990cited by this paper
• Small colloidal gold conjugated to Fab fragments or to immunoglobulin G as high-resolution labels for electron microscopy: a technical overview.

  1990cited by this paper
• An enhanced method for post-embedding immunocytochemical staining which preserves cell membranes.

  1990cited by this paper
• New aspects of bacterial ultrastructure as revealed by modern acrylics for electron microscopy.

  1990cited by this paper
• Immuno- and affinity probes for electron microscopy: a review of labeling and preparation techniques.

  1990cited by this paper
• Subcellular Localization of Chitinase and of Its Potential Substrate in Tomato Root Tissues Infected by Fusarium oxysporum f. sp. radicis-lycopersici.

  1990cited by this paper
• Quantitative aspects of labelling colloidal gold with proteins in immunocytochemistry

  1990cited by this paper
• NEW TISSUE FIXATION METHOD FOR CYTOCHEMISTRY USING MICROWAVE IRRADIATION

  1990cited by this paper
• Endocytosis mediated by the mannose receptor in liver endothelial cells. An immunocytochemical study.

  1990cited by this paper
• Freeze‐substitution without aldehyde or osmium fixatives: ultrastructure and implications for immunocytochemistry

  1990cited by this paper
• Routine use of backscattered electron imaging to visualize cytochemical and autoradiographic reactions in semi-thin plastic sections.

  1990cited by this paper
• Morpho-cytochemical and biochemical evidence for insulin absorption by the rat ileal epithelium

  1990cited by this paper
• Contact dermatitis from acrylate and methacrylate compounds in Lowicryl® embedding media for electron microscopy

  1990cited by this paper
• Quantitative immunocytochemical studies of endogenous albumin in rat aortic endothelial and mesothelial cells

  1990cited by this paper
• Immunolabeling efficiency of protein A-gold complexes.

  1990cited by this paper
• Sensitive solid-phase immune electron microscopy double-antibody technique with gold-immunoglobulin G complexes for detecting rotavirus in cell culture and feces

  1990cited by this paper
• Occupational risks of (meth)acrylate compounds in embedding media for electron microscopy

  1990cited by this paper
• Immunolocalization in three dimensions: immunogold staining of cytoskeletal and nuclear matrix proteins in resinless electron microscopy sections.

  1990cited by this paper
• Distribution of endogenous albumin in the glomerular wall of proteinuric patients.

  1990cited by this paper
• Physics of microwave technology in histochemistry

  1990cited by this paper
• Analysis of colloidal gold probes by isoelectric focusing in agarose gels.

  1990cited by this paper
• Localization and quantification of hormones, ligands, and mRNA with affinity-gold probes.

  1989cited by this paper
• Use of dinitrophenol-IgG conjugates to detect sparse antigens by immunogold labeling.

  1989cited by this paper
• Ultrastructural localization of insulin and C-peptide antigenic sites in rat pancreatic B cell obtained by applying the quantitative high-resolution protein A-gold approach.

  1989cited by this paper
• Use of poly(vinylpyrrolidone) and poly(vinyl alcohol) for cryoultramicrotomy

  1989cited by this paper
• Immunolabeling with the protein A-gold technique: an overview.

  1989cited by this paper
• Immunoelectron microscopic diagnosis in nephropathology.

  1989cited by this paper
• Ultrastructural localization of viral DNA in thin sections of herpes simplex virus type 1 infected cells by in situ hybridization.

  1989cited by this paper

• Immunoelectron microscopy: a comprehensive guide from sample preparation to high-resolution imaging

  2025cites this paper
• Techniques for Selective Labeling of Molecules and Subcellular Structures for Cryo-Electron Tomography

  2025cites this paper
• Interactome Mapping Reveals the Evolutionary History of the Nuclear Pore Complex

  2016cites this paper
• Trafficking Dynamics of PCSK9-Induced LDLR Degradation: Focus on Human PCSK9 Mutations and C-Terminal Domain

  2016cites this paper
• GRP94 Regulates Circulating Cholesterol Levels through Blockade of PCSK9-Induced LDLR Degradation.

  2015cites this paper
• Refinement of collagen-mineral interaction: a possible role for osteocalcin in apatite crystal nucleation, growth and development.

  2015cites this paper
• Comparison of the capacity of enamel matrix derivative gel and enamel matrix derivative in liquid formulation to adsorb to bone grafting materials.

  2015cites this paper
• Characterisation of secretory calcium-binding phosphoprotein-proline-glutamine-rich 1: a novel basal lamina component expressed at cell-tooth interfaces

  2014cites this paper
• Regulation of P-glycoprotein by Nuclear Receptors at the Blood-brain Barrier: Relevance to Human Immunodeficiency Virus-1 Pharmacotherapy

  2014cites this paper
• Perspectives on low voltage transmission electron microscopy as applied to cell biology

  2014cites this paper
• ARF1 regulates the Rho/MLC pathway to control EGF-dependent breast cancer cell invasion

  2014cites this paper
• Low Voltage Transmission Electron Microscopy in Cell Biology

  2013cites this paper
• Immunocytochemistry Introduction of a Novel HRP Substrate-Nanogold Probe for Signal Amplification in

  2013cites this paper
• ARF 1 regulates the Rho / MLC pathway to control EGF-dependent breast cancer cell invasion

  2013cites this paper
• Environmental scanning electron microscopy gold immunolabeling in cell biology.

  2013cites this paper
• Conformation and activity of glucose oxidase on homogeneously coated and nanostructured surfaces.

  2013cites this paper
• Development of a colorimetric protein array for the detection of sepsis

  2012cites this paper
• Poly(acrylic acid)-poly(ethylene glycol) layers on positively charged surface coatings: molecular structure, protein resistance, and application to single protein deposition.

  2012cites this paper
• Odontogenic ameloblast-associated and amelotin are novel basal lamina components

  2012cites this paper
• Adsorption of enamel matrix proteins to a bovine-derived bone grafting material and its regulation of cell adhesion, proliferation, and differentiation.

  2012cites this paper
• Compartmentalization of Pancreatic Secretory Zymogen Granules as Revealed by Low-Voltage Transmission Electron Microscopy

  2011cites this paper
• Psammomys obesus, a particularly important animal model for the study of the human diabetic nephropathy

  2011cites this paper
• Receptor-Mediated Transcytosis of Leptin through Human Intestinal Cells In Vitro

  2010cites this paper
• Expression profile of the embryonic markers nanog, OCT‐4, SSEA‐1, SSEA‐4, and frizzled‐9 receptor in human periodontal ligament mesenchymal stem cells

  2010influential citation
• Cross-talk between adipose and gastric leptins for the control of food intake and energy metabolism.

  2010cites this paper
• Localization, function and regulation of the two intestinal fatty acid-binding protein types

  2009cites this paper
• Association of AMP-activated Protein Kinase Subunits With Glycogen Particles as Revealed In Situ by Immunoelectron Microscopy

  2009cites this paper
• Up‐regulation of P‐glycoprotein by HIV protease inhibitors in a human brain microvessel endothelial cell line

  2009cites this paper
• Immunocytochemistry of matrix proteins in calcified tissues: functional biochemistry on section.

  2009cites this paper
• Modulation of intestinal cholesterol absorption by high glucose levels: impact on cholesterol transporters, regulatory enzymes, and transcription factors.

  2008cites this paper
• Cell surface protein detection with immunogold labelling in ESEM: Optimisation of the method and semi‐quantitative analysis

  2008cites this paper
• Glomerular CD34 Expression in Short- and Long-term Diabetes

  2008cites this paper
• Low‐voltage transmission electron microscopy reveals SV40 viral particles within secretory granules in pancreatic cells

  2008cites this paper
• Ameloblastin and amelogenin share a common secretory pathway and are co-secreted during enamel formation.

  2008cites this paper
• Pancreatic Acinar and Islet Cell Infection by Low-Dose SV40 Administration

  2008cites this paper
• Visualization of nuclear organization by ultrastructural cytochemistry.

  2008cites this paper
• Adiponectin stimulates phosphorylation of AMP-activated protein kinase α in renal glomeruli

  2008cites this paper
• In Vivo Functional Analysis of Polyglutamic Acid Domains in Recombinant Bone Sialoprotein

  2007cites this paper
• Biological role, protein expression, subcellular localization, and oxidative stress response of paraoxonase 2 in the intestine of humans and rats.

  2007cites this paper
• Bactericidal efficacy of liposomal aminoglycosides against Burkholderia cenocepacia.

  2007cites this paper
• Localization of P-glycoprotein at the nuclear envelope of rat brain cells.

  2007cites this paper
• Leptin secretion by white adipose tissue and gastric mucosa.

  2007cites this paper
• Transcytosis of gastric leptin through the rat duodenal mucosa.

  2007cites this paper
• Expression and localization of MT1-MMP and furin in the glomerular wall of short- and long-term diabetic rats.

  2006cites this paper
• On the nature of obligate intracellular symbiosis of rickettsiae — Rickettsia prowazekii cells import mitochondrial porin

  2006cites this paper
• Pattern of mineralization after regenerative periodontal therapy with enamel matrix proteins.

  2006cites this paper
• Alteration of the pancreatic endocrine component in the early stage of acute necrotic pancreatitis in rats.

  2006cites this paper
• Localization and role of NPC1L1 in cholesterol absorption in human intestine Published, JLR Papers in Press, July 7, 2006.

  2006cites this paper
• Leptin biosynthetic pathway in white adipocytes.

  2006cites this paper
• Internalization and Transcytosis of Pancreatic Enzymes by the Intestinal Mucosa

  2006cites this paper
• Secretion of soluble leptin receptors by exocrine and endocrine cells of the gastric mucosa.

  2006cites this paper
• In Situ Localization of P-glycoprotein (ABCB1) in Human and Rat Brain

  2006cites this paper
• Regionally specific distribution of the binding of anti‐glutamine synthetase and anti‐S100 antibodies and of Datura stramonium lectin in glial domains of the optic lobe of the giant prawn

  2006cites this paper
• Mechanism of Enhanced Activity of Liposome-Entrapped Aminoglycosides against Resistant Strains of Pseudomonas aeruginosa

  2006cites this paper
• Immunocytochemical Study of Amelogenin Deposition during the Early Odontogenesis of Molars in Alendronate-treated Newborn Rats

  2006cites this paper
• Malonyl‐CoA decarboxylase is present in the cytosolic, mitochondrial and peroxisomal compartments of rat hepatocytes

  2005cites this paper
• Endocrine and Exocrine Secretion of Leptin by the Gastric Mucosa

  2005influential citation
• Alteration of chaperonin60 and pancreatic enzyme in pancreatic acinar cell under pathological condition.

  2005cites this paper
• Sequence of protein expression of bone sialoprotein and osteopontin at the developing interface between repair cementum and dentin in human deciduous teeth

  2005cites this paper
• Differential polymerization of the two main protein components of dragline silk during fibre spinning

  2005cites this paper
• Developmental appearance of dentin matrix protein 1 during the early dentinogenesis in rat molars as identified by high-resolution immunocytochemistry

  2005cites this paper
• Expression de PSA-NCAM dans le système nerveux entérique chez le rat au cours du développement et au cours de la réponse inflammatoire

  2005cites this paper
• Evaluation of Pancreatic Amylase mRNA upon Cholinergic Stimulation of Secretion

  2005influential citation
• Identification of microsomal triglyceride transfer protein in intestinal brush-border membrane.

  2004cites this paper
• The proprotein convertase furin colocalizes with caveolin-1 in the Golgi apparatus and endosomes of hepatocytes

  2004influential citation
• Nanotexturing of titanium-based surfaces upregulates expression of bone sialoprotein and osteopontin by cultured osteogenic cells.

  2004cites this paper
• Sorting of Furin in Polarized Epithelial and Endothelial Cells: Expression Beyond the Golgi Apparatus

  2004cites this paper
• Type VI collagen in glomeruli of short- and long-term experimental diabetic rats

  2004cites this paper
• Microwave Irradiation of Ethanol-fixed Bone Improves Preservation, Reduces Processing Time, and Allows Both Light and Electron Microscopy on the Same Sample

  2004cites this paper
• Ontogeny, immunolocalisation, distribution and function of SR-BI in the human intestine

  2004cites this paper
• Cellular localization and functional expression of P‐glycoprotein in rat astrocyte cultures

  2004cites this paper
• Rapid in vivo transport of proteins from digested allergen across pre-sensitized gut.

  2004cites this paper
• Hertwig's epithelial root sheath, enamel matrix proteins, and initiation of cementogenesis in porcine teeth.

  2004cites this paper
• Atomic force spectroscopy-based study of antibody pesticide interactions for characterization of immunosensor surface.

  2004cites this paper
• Immunogold Detection of Co-localized Neuropeptides: Methodological Aspects

  2004cites this paper
• Redistribution of Integrins in Tubular Epithelial Cells during Diabetic Glycogen Nephrosis

  2004cites this paper
• A Tracer Study with Systemically and Locally Administered Dinitrophenylated Osteopontin

  2004cites this paper
• Expression differences in mitochondrial and secretory chaperonin 60 (Cpn60) in pancreatic acinar cells

  2003influential citation
• A solid-phase method for evaluation of gold conjugate used in quantitative detection of antigen by immunogold-labeling electron microscopy.

  2003cites this paper
• Furin interacts with proMT1-MMP and integrin αV at specialized domains of renal cell plasma membrane

  2003cites this paper
• Angiotensinogen localization and secretion in the rat pancreas.

  2003cites this paper
• Morphofunctional Studies of the Glomerular Wall in Mice Lacking Entactin-1

  2003cites this paper
• Apoptosis of Tubular Epithelial Cells in Glycogen Nephrosis During Diabetes

  2003cites this paper
• Circulating bile salt-dependent lipase originates from the pancreas via intestinal transcytosis.

  2003cites this paper
• A new porous hydroxyapatite for promotion of bone regeneration in maxillary sinus augmentation: clinical and histologic study in humans.

  2003cites this paper
• Ultrastructural and cytochemical study of cell wall modification by lactoferrin, lactoferricin and penicillin G against Staphylococcus aureus.

  2003cites this paper
• The normal liver harbors the vitamin D nuclear receptor in nonparenchymal and biliary epithelial cells

  2003cites this paper
• Immunocytochemical characterization of ectopic enamel deposits and cementicles in human teeth.

  2003cites this paper
• Quantitative Immunogold Labeling of Bone Sialoprotein and Osteopontin in Methylmethacrylate-embedded Rat Bone

  2003influential citation
• Circulating Glycated Albumin and Glomerular Anionic Charges

  2003cites this paper
• Morpho-functional studies of the blood-brain barrier in streptozotocin-induced diabetic rats

  2002cites this paper
• Optimization of Immunogold Labeling TEM

  2002cites this paper
• Immunodetection of Osteopontin at Sites of Resorption in the Pulp of Rat Molars

  2002cites this paper
• Pre‐screening for antigen detectability in cells: a TEM‐based solid phase digital immunogold detection method utilizing ultra low volumes of reagents

  2002cites this paper
• 28 Electron microscopy in cellular microbiology: Ultrastructural methods for the study of cell surface antigens and pathogenic host—Cell interactions

  2002cites this paper
• Functional expression and localization of P‐glycoprotein at the blood brain barrier

  2002cites this paper
• Morphological and cytochemical aspects of capillary permeability

  2002influential citation
• Localization of Microsomal Triglyceride Transfer Protein in the Golgi

  2002cites this paper
• Intracellular localization of the particulate methane monooxygenase and methanol dehydrogenase in Methylomicrobium album BG8

  2002cites this paper
• Pituitary Adenoma in Carney Complex: An Immunohistochemical, Ultrastructural, and Immunoelectron Microscopic Study

  2002cites this paper