Cryofixation, cryosubstitution, cryoembedding for ultrastructural, immunocytochemical and microanalytical studies.

No abstract is available for this paper.

• Publication year

  1994

• Venue

  Micron

• Publication date

  Unknown publication date

• Fields of study

  Biology, Medicine, Materials Science

• Identifiers
  DOI 10.1016/0968-4328(94)90056-6PMID 8069612
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Electron-microscopic demonstration of olfactory-marker protein with protein G-gold in freeze-substituted, Lowicryl K11M-embedded rat olfactory-receptor cells

  2004cited by this paper
• Application of cryosubstitution in neurohormone-and neurotransmitter-immunocytochemistry

  2004cited by this paper
• Programmable freeze‐substitution and cryo‐embedding device

  1993cited by this paper
• Preservation of antigenic reactivity after cryofixation, cryosubstitution, and cryoembedding in Lowicryl HM23: Application to alcohol dehydrogenase in Drosophila fat body

  1993cited by this paper
• Immunodetection of low concentrations of ovalbumin in cryoprocessed quail oviduct cells by semi‐automatic quantitative analysis

  1993cited by this paper
• In situ conservation of diffusible elements in liver cells after cryofixation, cryosubstitution, and low temperature embedding at 193 K in HM23 Lowicryl resin

  1993cited by this paper
• Low-Temperature Microscopy and Analysis

  1992cited by this paper
• The ultrastructure of the chromosome periphery in human cell lines

  1992cited by this paper
• Ultrastructural studies on membrane, cytoskeletal, mucous, and protective compartments in olfaction.

  1992cited by this paper
• Lectins bind differentially to cilia and microvilli of major and minor cell populations in olfactory and nasal respiratory epithelia

  1992cited by this paper
• Comparison of cryopreparation techniques for electron probe microanalysis of cells as exemplified by human erythrocytes.

  1992cited by this paper
• Ultrastructural localization of olfactory transduction components: the G protein subunit Golf alpha and type III adenylyl cyclase.

  1992cited by this paper
• Freeze Substitution and Low-Temperature Embedding

  1992cited by this paper
• An improved cryofixation method: cryoquenching of small tissue blocks during microwave irradiation

  1992cited by this paper
• New applications of multivariate statistical analysis in spectroscopy and microscopy

  1992cited by this paper
• Three‐dimensional imaging of the mucus secretory process in the cryofixed frog respiratory epithelium

  1991cited by this paper
• Freeze-substitution and Lowicryl HM20 embedding of fixed rat brain: suitability for immunogold ultrastructural localization of neural antigens.

  1991cited by this paper
• Versatile controlling system for cryopreparation techniques in electron microscopy.

  1991cited by this paper
• Freeze‐substitution and the preservation of diffusible ions

  1991cited by this paper
• Post-embedding immunoelectron microscopy of tissue processed by rapid freezing and freeze-substitution without chemical fixatives.

  1991cited by this paper
• Suggestions for the quantitative X‐ray microanalysis of thin sections of frozen‐dried and embedded biological tissues

  1991cited by this paper
• (3‐Cryo) methods (cryofixation, cryosubstitution and cryoembedding) for processing of tissues for ultrastructural and immunocytochemical studies. Application to oviduct cells of laying quail

  1991cited by this paper
• Immunoelectron microscopy of acetylcholine receptors and 43 KD protein after rapid freezing, freeze-substitution, and low-temperature embedding in lowicryl K11M.

  1991cited by this paper
• Design and operation of a simple environmental chamber for rapid freezing fixation.

  1991cited by this paper
• Cryopreparation of tissue for clinical applications of X-ray microanalysis.

  1991cited by this paper
• X-ray microanalysis of cell nuclei.

  1991cited by this paper
• The potential of cryofixation and freeze substitution: observations and theoretical considerations

  1991cited by this paper
• Cryofixation, cryosubstitution, cryo‐embedding for visualizing of nuclear ultrastructure and for immunodetection HeLa cells

  1991cited by this paper
• Advantages of fast-freeze fixation followed by freeze-substitution for the preservation of cell integrity.

  1991cited by this paper
• Some applications of cryosubstitution in ultrastructural studies of the cell nucleus

  1991cited by this paper
• Cryopreparation of tissue for clinical applications of X-ray microanalysis.

  1991cited by this paper
• Cryofixation and ultra‐low‐temperature freeze‐drying as a preparative technique for TEM

  1991cited by this paper
• Immunoelectron microscopy of tissues processed by rapid freezing and freeze-substitution fixation without chemical fixatives: application to catalase in rat liver hepatocytes.

  1990cited by this paper
• Cooling rate and ice‐crystal measurement in biological specimens plunged into liquid ethane, propane, and Freon 22

  1990cited by this paper
• Freeze‐substitution without aldehyde or osmium fixatives: ultrastructure and implications for immunocytochemistry

  1990cited by this paper
• Occupational risks of (meth)acrylate compounds in embedding media for electron microscopy

  1990cited by this paper
• A low temperature vacuum embedding procedure for X-ray microanalysis of biological specimens at subcellular level.

  1990cited by this paper
• Morphology of rat exocrine pancreas prepared by anhydrous cryo-procedures.

  1990cited by this paper
• Fine structure of unstained human chromosome fibres dried with no fixative as observed by X‐ray contact microscopy

  1990cited by this paper
• Ultrastructural preservation of nuclei and chromatin: improvement with low‐temperature methods

  1990cited by this paper
• Olfactory and nasal respiratory epithelia, and foliate taste buds visualized with rapid-freeze freeze-substitution and Lowicryl K11M embedding. Ultrastructural and initial cytochemical studies.

  1989cited by this paper
• Electron Probe Microanalysis in Pathology

  1989cited by this paper
• In situ localization of a foreign protein in transgenic plants by immunoelectron microscopy following high pressure freezing: freeze substitution and low temperature embedding

  1989cited by this paper
• Low-temperature Methods

  1989cited by this paper
• Multivariate statistical analysis of electron probe microanalytical data on cell nuclear constituents.

  1989cited by this paper
• Low Temperature Embedding

  1989cited by this paper
• Ensuring the Validity of Results in Biological X-Ray Microanalysis

  1989cited by this paper
• The application of cryo-ultramicrotomy and freeze-substitution in immuno-gold labelling of hybrid proteins in Escherichia coli. A comparison.

  1989cited by this paper
• The organization of myosin and actin in rapid frozen nerve growth cones

  1989cited by this paper
• 5 – Lowicryls and Low-Temperature Embedding for Colloidal Gold Methods

  1989cited by this paper
• Immunocytochemical localization of amylase in gerbil salivary gland acinar cells processed by rapid freezing and freeze-substitution fixation.

  1989cited by this paper
• Optimal preparatory procedures of cryofixation for immunocytochemistry.

  1989cited by this paper
• High pressure freezing comes of age.

  1989cited by this paper
• A Portable Type of Rapid Freezing Device for Metal Contact and Immersion Freezing

  1989cited by this paper
• Improved method for electron microscopy immunocytochemistry of muscle.

  1989cited by this paper
• Freeze-Substitution and Low Temperature Embedding for Analytical Electron Microscopy

  1989cited by this paper
• Influence of fixatives and embedding media on immunolabelling of freeze-substituted cells.

  1989cited by this paper
• Exocytosis of bovine chromaffin granules in Ficoll captured by rapid freezing.

  1989cited by this paper
• The control of temperature during polymerization of Lowicryl K4M: there is a low‐temperature embedding method

  1989cited by this paper
• X-ray microanalysis of freeze-dried and frozen-hydrated cryosections.

  1988cited by this paper
• Frontiers in electron probe microanalysis: application to cell physiology.

  1988cited by this paper
• Low temperature techniques for X-ray microanalysis in pathology: alternatives to cryoultramicrotomy.

  1988cited by this paper
• The state of water in the cell

  1988cited by this paper
• Cryo-electron microscopy of vitrified specimens

  1988cited by this paper
• Cryofixation Without Pretreatment at Ambient Pressure

  1987cited by this paper
• Immunogold Labelling of Cryosections and Cryofractures

  1987cited by this paper
• Freeze-Substitution and Freeze-Drying

  1987cited by this paper
• The fine structure of sublingual gland acinar cells of the Mongolian gerbil, Meriones unguiculatus, processed by rapid freezing followed by freeze-substitution fixation

  1987cited by this paper
• Secretory process of mucus-secreting cells in mouse colonic mucosa studied by rapid freezing and freeze-substitution.

  1987cited by this paper
• Theory and practice of high pressure freezing.

  1987cited by this paper
• Estimation of organelle water fractions from frozen‐dried cryosections

  1987cited by this paper
• Mouse Leydig cells processed by freeze-substitution, with particular reference to the lamellar arrangement of the smooth endoplasmic reticulum.

  1987cited by this paper
• Use of on-section immunolabeling and cryosubstitution for studies of bacterial DNA distribution

  1987cited by this paper
• Combined quick freezing, freeze‐drying, and embedding tissue at low temperature and in low viscosity resins

  1987cited by this paper
• The intracellular distribution of ions and water in rat liver and heart muscle

  1987cited by this paper
• Cryofixation of Diffusible Elements in Cells and Tissues for Electron Probe Microanalysis

  1987cited by this paper
• Physics of Water and Ice: Implications for Cryofixation

  1987cited by this paper
• High Resolution Metal Replication of Freeze-Dried Specimens

  1987cited by this paper
• The occurrence of metals Al, Fe, Ni, Cu, Zn in the nuclei of animal cells: an ultrastructural, in situ, X‐ray microanalytical study

  1987cited by this paper
• Cryotechniques in Biological Electron Microscopy

  1987cited by this paper
• The structure of cytoplasm in directly frozen cultured cells. II. Cytoplasmic domains associated with organelle movements

  1986cited by this paper
• A survey of ultra‐rapid cryofixation methods with particular emphasis on applications to freeze‐fracturing, freeze‐etching, and freeze‐substitution

  1986cited by this paper
• Freeze-dried embedded specimens for biological microanalysis.

  1986cited by this paper
• Z‐Contrast in Biology A Comparison with Other Imaging Modes a

  1986cited by this paper
• Distribution of microtubules within the intestinal terminal web as revealed by quick-freezing and cryosubstitution.

  1986cited by this paper
• Fast cryofixation technique for X‐ray microanalysis

  1986cited by this paper
• Developments of new Lowicryl® resins for embedding biological specimens at even lower temperatures

  1986cited by this paper
• An excursion through the ultrastructural world of quick-frozen pancreatic islets.

  1986cited by this paper
• Temperature control in Lowicryl K4M and glycol methacrylate during polymerization: is there a low‐temperature embedding method?

  1986cited by this paper
• Calcium content of mitochondria and endoplasmic reticulum in liver frozen rapidly in vivo

  1985cited by this paper
• Low Temperature Methods in Biological Electron Microscopy

  1985cited by this paper
• Two mucous cell types revisited after quick‐freezing and cryosubstitution

  1985cited by this paper
• Demonstration of an extensive trans-tubular network continuous with the Golgi apparatus stack that may function in glycosylation.

  1985cited by this paper
• Low temperature embedding with Lowicryl resins: two new formulations and some applications

  1985cited by this paper
• Shape and fine structure of nucleoids observed on sections of ultrarapidly frozen and cryosubstituted bacteria

  1985cited by this paper
• A comparison of subcellular element concentrations in frozen-dried, plastic-embedded, dry-cut sections and frozen-dried cryosections.

  1985cited by this paper
• The structure of cytoplasm in directly frozen cultured cells. I. Filamentous meshworks and the cytoplasmic ground substance

  1984cited by this paper
• Cartilage ultrastructure after high pressure freezing, freeze substitution, and low temperature embedding. I. Chondrocyte ultrastructure--implications for the theories of mineralization and vascular invasion

  1984cited by this paper
• Freeze drying and freeze substitution combined with low temperature-embedding

  1984cited by this paper
• Extraction of lipids during freeze‐substitution of Acholeplasma laidlawii‐cells for electron microscopy

  1984cited by this paper

• Ultraplex microscopy: versatile highly-multiplexed molecular labeling and imaging across scale and resolution

  2024cites this paper
• Guide for collecting and reporting metadata on protocol variables and parameters from slide-based histotechnology assays to enhance reproducibility

  2022cites this paper
• New insights on Laminaria digitata ultrastructure through combined conventional chemical fixation and cryofixation

  2021cites this paper
• Low Temperature Embedding of Chemically Unfixed Biological Material After Cryosorption Freeze-Drying

  2021cites this paper
• Subcellular Localization of Copper—Cellular Bioimaging with Focus on Neurological Disorders

  2020cites this paper
• Essential Methods of Plant Sample Preparation for Light Microscopy.

  2019cites this paper
• Plant Cell Morphogenesis

  2019cites this paper
• Particle arrangements in clay slurries: The case against the honeycomb structure

  2018cites this paper
• Photons in - numbers out: perspectives in quantitative fluorescence microscopy for in situ protein counting

  2018cites this paper
• Electron microscopy approach for the visualization of the epithelial and endothelial glycocalyx.

  2017cites this paper
• Development of an ultrastructure preservation protocol for platelet and fibrin networks utilizing high pressure freezing and subsequent comparison of new and established protocols

  2016cites this paper
• Imaging trace element distributions in single organelles and subcellular features

  2016cites this paper
• Cryo-planing of frozen-hydrated samples using cryo triple ion gun milling (CryoTIGM™).

  2015cites this paper
• Coherent X-Ray Diffractive Imaging on the Single-Cell-Level of Microbial Samples:

  2015influential citation
• Essential methods of plant sample preparation for light microscopy.

  2014cites this paper
• Correlative microscopy methods that maximize specimen fidelity and data completeness, and improve molecular localization capabilities.

  2013cites this paper
• Nanoimaging cells using soft X-ray tomography.

  2013cites this paper
• A simple cryo‐holder facilitates specimen observation under a conventional scanning electron microscope

  2012cites this paper
• Freeze substitution followed by low melting point wax embedding preserves histomorphology and allows protein and mRNA localization techniques

  2011cites this paper
• Applicable technical method for lower temperature freeze-substitution of cryo-fixed cells

  2011cites this paper
• A study on new approaches in coherent x-ray microscopy of biological specimens

  2011cites this paper
• A new cryo-STEM method for imaging food colloids in the scanning electron microscope.

  2010cites this paper
• Electron microscopy of viruses.

  2010cites this paper
• Could a dysfunction of ferritin be a determinant factor in the aetiology of some neurodegenerative diseases?

  2010cites this paper
• Saccharomyces cerevisiae nuclear and nucleolar antigen preservation for immunoelectron microscopy.

  2010cites this paper
• Visualization of proteins in intact cells with a clonable tag for electron microscopy.

  2009cites this paper
• Microautoradiographic localisation of [3H]sucrose and [3H]mannitol in Robinia pseudoacacia pulvinar tissues during phytochrome-mediated nyctinastic closure

  2006cites this paper
• Electron Microscopy in Cell Biology

  2006cites this paper
• Microscopy methods applied to research on cyanobacteria

  2004cites this paper
• An Improved Chemical Fixation Method Suitable for Immunogold Localization of Green Fluorescent Protein in the Golgi Apparatus of Tobacco Bright Yellow (BY-2) Cells

  2003cites this paper
• Electron microscopical enzyme histochemistry on unfixed tissues and cells. Bridging the gap between LM and EM enzyme histochemistry.

  2003cites this paper
• Preparative Techniques for Transmission Electron Microscopy and Confocal Laser Scanning Microscopy of Lichens

  2002cites this paper
• The significance of the Golgi complex in envelopment of bovine herpesvirus 1 (BHV-1) as revealed by cryobased electron microscopy.

  2002cites this paper
• Enhanced resolution of membranes in cultured cells by cryoimmobilization and freeze‐substitution

  2001cites this paper
• Morphologic and functional characterization of caveolae in rat liver hepatocytes

  2001cites this paper
• Comparisons of the resurrection grass, Eragrostis nindensis, with the related desiccation-sensitive species, E. curvula

  2001cites this paper
• The fine structure of acidocalcisomes in Trypanosoma cruzi

  2000cites this paper
• PC12 Cells Have Caveolae That Contain TrkA

  2000cites this paper
• Unusual ultrastructural features in three strains of Cyanothece (cyanobacteria)

  2000influential citation
• Distribution of brominated compounds within the sponge Aplysina aerophoba: coupling of X-ray microanalysis with cryofixation techniques

  2000influential citation
• Freezing and low - temperature embedding

  1999cites this paper
• Calmodulin Binds to p21Cip1 and Is Involved in the Regulation of Its Nuclear Localization*

  1999cites this paper
• A comparison of cryo- versus chemical fixation in the soil green algae Jaagiella.

  1998cites this paper
• Novel Entry Pathway of Bovine Herpesvirus 1 and 5

  1998cites this paper
• Two Types of Virus-Related Particles Are Found during Transmissible Gastroenteritis Virus Morphogenesis

  1998cites this paper
• Optimization of phosphorus localization by EFTEM of nucleic acid containing structures

  1998cites this paper
• Immunodissection of the connective tissue matrix in human skin

  1997cites this paper
• Spray‐freezing freeze substitution (SFFS) of cell suspensions for improved preservation of ultrastructure

  1997cites this paper
• Cryofixation, cryosubstitution, and immunoelectron microscopy: potential role in diagnostic pathology.

  1996cites this paper
• Direct measurement of elemental distributions in insulin-producing cells using nuclear microscopy.

  1996cites this paper
• Cryofixing single cells and multicellular specimens enhances structure and immunocytochemistry for light microscopy

  1996cites this paper
• Multiple labeling in electron microscopy: its application in cardiovascular research.

  1996cites this paper
• Freeze‐Fracture, Deep‐Etch, and Freeze‐Substitution Studies of Olfactory Epithelia, With Special Emphasis on Immunocytochemical Variables

  1995cites this paper
• High-pressure freeze fixation reveals novel features during ontogenesis of the vegetative cell in Ledebouria pollen: an ultrastructural and cytochemical study.

  1995cites this paper
• Colloidal gold post-embedding immunocytochemistry.

  1995cites this paper
• Ion dynamics in cells-preparation for studies of intracellular processes

  1995cites this paper
• Multivariate statistical analysis applied to X-ray spectra and X-ray mapping of liver cell nuclei.

  1994cites this paper
• Compartmentalization of secretory proteins in pancreatic zymogen granules as revealed by immunolabeling on cryo‐fixed and molecular distillation processed tissue *

  1994cites this paper
• Comparative study of freeze‐substitution techniques for x‐ray microanalysis of biological tissue

  1994cites this paper