Yeast RNA polymerase II subunit RPB9 is essential for growth at temperature extremes.

The Saccharomyces cerevisiae RNA polymerase II subunit gene RPB9 was isolated and sequenced. RPB9 is a single copy gene on chromosome VII. The RPB9 sequence predicts a protein of 122 amino acids with a molecular mass of 14,200 Da. The yeast RPB9 subunit is similar in size and sequence to a protein encoded by DNA adjacent to the suppressor of the Hairy Wing gene in Drosophila melanogaster. Deletion of the RPB9 gene produced cells that were heat- and cold-sensitive. The RPB9 subunit, like the previously described RNA polymerase II subunit RPB4, is not essential for synthesis of mRNA, but is required for normal cell growth over a wide temperature range.

• Publication year

  1991

• Venue

  Journal of Biological Chemistry

• Publication date

  1991-10-05

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/s0021-9258(18)55171-1PMID 1918023
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• The yeast RPB9 subunit is similar in size and sequence to a Drosophila melanogaster protein encoded by DNA adjacent to the suppressor of the Hairy Wing gene.

  Confidence 0.88

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• RPB9 is not essential for mRNA synthesis but is required for normal cell growth over a wide temperature range.

  Confidence 0.97

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• Deletion of RPB9 produces heat- and cold-sensitive cells.

  Confidence 0.95

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• RPB9 is a single-copy gene on chromosome VII and encodes a protein of 122 amino acids with an अनुमानित molecular mass of 14,200 Da.

  Confidence 0.98

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review

• 122 amino acids

  protein length

  The predicted length of the RPB9-encoded protein in amino acid residues.

  Aliases: 122 aa

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• 14,200 da

  molecular mass

  The predicted molecular mass of the RPB9-encoded protein.

  Aliases: 14.2 kDa

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• chromosome vii

  chromosome

  The yeast chromosome reported as the genomic location of the RPB9 gene.

  Aliases: chromosome 7

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• drosophila melanogaster

  organism

  The fruit fly species used for the cross-species sequence comparison involving a related protein.

  Aliases: fruit fly

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• heat- and cold-sensitive

  phenotype

  A temperature-dependent growth phenotype observed after deletion of RPB9.

  Aliases: temperature-sensitive

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• mrna synthesis

  cellular process

  The cellular process of messenger RNA production by RNA polymerase II referenced in the gene-function assessment.

  Aliases: messenger RNA synthesis

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• normal cell growth over a wide temperature range

  phenotype

  The growth behavior of yeast cells across broad temperatures that depends on RPB9.

  Aliases: growth over a wide temperature range

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• protein adjacent to the suppressor of the hairy wing gene

  protein

  A Drosophila protein encoded by DNA next to the suppressor of the Hairy Wing gene that is compared with yeast RPB9.

  Aliases: SHW-adjacent protein

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• rpb9 gene

  gene

  The Saccharomyces cerevisiae gene that encodes the RNA polymerase II subunit RPB9.

  Aliases: RPB9

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review

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