Redox-dependent conformational changes in cytochrome C oxidase suggest a gating mechanism for proton uptake.

No abstract is available for this paper.

• Publication year

  2009

• Venue

  Biochemistry

• Publication date

  2009-06-16

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1021/bi9001387PMID 19397279PMCID PMC2720787
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2007influential reference
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  2006influential reference
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  2001cited by this paper
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  2001cited by this paper
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  1999cited by this paper
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  1998cited by this paper
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  1998cited by this paper
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  1998cited by this paper
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  1998cited by this paper
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  1998cited by this paper
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  1998cited by this paper
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  1997cited by this paper
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  1997cited by this paper
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  1997cited by this paper
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  1997cited by this paper
• Processing of X-ray diffraction data collected in oscillation mode.

  1997cited by this paper
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  1996cited by this paper
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  1995cited by this paper
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  1995cited by this paper
• The low ionic strength crystal structure of horse cytochrome c at 2.1 A resolution and comparison with its high ionic strength counterpart.

  1995cited by this paper
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  1994cited by this paper
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  1994cited by this paper
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  1992cited by this paper
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  1981cited by this paper
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  1981cited by this paper
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  1977cited by this paper
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  1972cited by this paper

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