MiR-519d impedes cisplatin-resistance in breast cancer stem cells by down-regulating the expression of MCL-1

Cancer stem cells are considered as the cell population which is responsible for chemoresistance and treatment failure in breast cancer patients. Therefore, it is urgent to explore the mechanism by which cancer stem cells survive under the treatment of chemotherapeutic drugs such as cisplatin. In this paper, we demonstrated significant decrease of miR-519d in breast cancer stem cells by quantitative RT-PCR analysis. Furthermore, we found the enforced expression of miR-519d in T-47D-cancer stem cells significantly increased their sensitivity to cisplatin through the apoptosis pathway. In addition, the gene of MCL-1, which is a member of pro-apoptotic Bcl-2 family, was found to be the target of miR-519d in T-47D-cancer stem cells. Our date demonstrated that enforced miR-519d expression enhanced the cisplatin-induced apoptosis through the MCL-1 dependent mitochondria pathway in breast cancer stem cells. Taken together, the present study suggests that miR-519d reduces chemoresistance in breast cancer stem cells, and understanding of miR-519d may be helpful for increasing the efficacy of chemotherapy.

• Publication year

  2017

• Venue

  OncoTarget

• Publication date

  2017-02-28

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.18632/oncotarget.15781PMID 28423543PMCID 5400641
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• miR-519d enhances cisplatin-induced apoptosis through an MCL-1-dependent mitochondria pathway in breast cancer stem cells.

  Confidence 0.93

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewq (76h6bfydm6) review
• MCL-1 is identified as a miR-519d target in T-47D cancer stem cells.

  Confidence 0.97

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewq (76h6bfydm6) review
• Enforced expression of miR-519d increases cisplatin sensitivity in T-47D cancer stem cells through an apoptosis pathway.

  Confidence 0.95

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewq (76h6bfydm6) review
• miR-519d is significantly decreased in breast cancer stem cells.

  Confidence 0.98

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewq (76h6bfydm6) review

• apoptosis pathway

  pathway

  A programmed cell-death signaling pathway associated with drug response in the experiments.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewq (76h6bfydm6) review
• breast cancer stem cells

  cell population

  A breast cancer subpopulation with stem-cell-like properties used as the resistant cell model.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewq (76h6bfydm6) review
• cisplatin

  drug

  A platinum-based chemotherapeutic drug used to assess drug sensitivity and apoptosis.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewq (76h6bfydm6) review
• mcl-1

  gene, protein

  A Bcl-2 family gene and protein involved in apoptosis regulation and analyzed in the breast cancer stem cell model.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewq (76h6bfydm6) review
• mir-519d

  microRNA

  A microRNA examined for its role in regulating cisplatin response in breast cancer stem cells.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewq (76h6bfydm6) review
• mitochondria pathway

  pathway

  The mitochondrial apoptosis signaling route implicated in cisplatin-induced cell death.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewq (76h6bfydm6) review

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