Parallel multiplicative target screening against divergent bacterial replicases: identification of specific inhibitors with broad spectrum potential.

No abstract is available for this paper.

• Publication year

  2010

• Venue

  Biochemistry

• Publication date

  2010-03-23

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1021/bi9020764PMID 20184361PMCID PMC2849275
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Quinazolin-2-ylamino-quinazolin-4-ols as novel non-nucleoside inhibitors of bacterial DNA polymerase III.

  2009cited by this paper
• Structure of a small-molecule inhibitor of a DNA polymerase sliding clamp

  2008cited by this paper
• A method to assay inhibitors of DNA polymerase IIIC activity.

  2008cited by this paper
• Exchange of DNA polymerases at the replication fork of bacteriophage T7

  2007cited by this paper
• The role of mitochondria in pharmacotoxicology: a reevaluation of an old, newly emerging topic.

  2007cited by this paper
• Late stage antibacterial drugs in the clinical pipeline.

  2007cited by this paper
• Dihydrofolate reductase inhibitors as antibacterial agents.

  2006cited by this paper
• Staphylococcus aureus Helicase but Not Escherichia coli Helicase Stimulates S. aureus Primase Activity and Maintains Initiation Specificity

  2006cited by this paper
• Crystal structure of the catalytic alpha subunit of E. coli replicative DNA polymerase III.

  2006cited by this paper
• A novel processive mechanism for DNA synthesis revealed by structure, modeling and mutagenesis of the accessory subunit of human mitochondrial DNA polymerase.

  2006cited by this paper
• The structure of T. aquaticus DNA polymerase III is distinct from eukaryotic replicative DNA polymerases.

  2006cited by this paper
• Determination of Selectivity and Efficacy of Fatty Acid Synthesis Inhibitors*

  2005cited by this paper
• A homogeneous, high-throughput fluorescence resonance energy transfer-based DNA polymerase assay.

  2005cited by this paper
• Interaction between the T4 helicase-loading protein (gp59) and the DNA polymerase (gp43): a locking mechanism to delay replication during replisome assembly.

  2005cited by this paper
• Cellular DNA replicases: components and dynamics at the replication fork.

  2005cited by this paper
• Bacterial topoisomerase inhibitors: quinolone and pyridone antibacterial agents.

  2005cited by this paper
• Mechanism of Loading the Escherichia coli DNA Polymerase III Sliding Clamp

  2004cited by this paper
• DNA polymerase gamma, the mitochondrial replicase.

  2004cited by this paper
• Small molecule modulation of the human chromatid decatenation checkpoint.

  2003cited by this paper
• Development and Use of a High-Throughput Bacterial DNA Gyrase Assay to Identify Mammalian Topoisomerase II Inhibitors with Whole-Cell Anticancer Activity

  2003cited by this paper
• Mechanism of loading the Escherichia coli DNA polymerase III beta sliding clamp on DNA. Bona fide primer/templates preferentially trigger the gamma complex to hydrolyze ATP and load the clamp.

  2003cited by this paper
• Competitive processivity‐clamp usage by DNA polymerases during DNA replication and repair

  2003cited by this paper
• Mechanism of Loading the Escherichia coli DNA Polymerase III β Sliding Clamp on DNA

  2003cited by this paper
• Chromosomal replicases as asymmetric dimers: studies of subunit arrangement and functional consequences

  2003cited by this paper
• Evidence that monastrol is an allosteric inhibitor of the mitotic kinesin Eg5.

  2002cited by this paper
• A common mechanism underlying promiscuous inhibitors from virtual and high-throughput screening.

  2002cited by this paper
• An expanded view of bacterial DNA replication

  2002cited by this paper
• Evolution of DNA Polymerase Families: Evidences for Multiple Gene Exchange Between Cellular and Viral Proteins

  2002cited by this paper
• PolC-type polymerase III of Streptococcus pyogenes and its use in screening for chemical inhibitors.

  2002cited by this paper
• tau binds and organizes Escherichia coli replication through distinct domains. Partial proteolysis of terminally tagged tau to determine candidate domains and to assign domain V as the alpha binding domain.

  2001cited by this paper
• τ Binds and Organizes Escherichia coli Replication Proteins through Distinct Domains

  2001cited by this paper
• The DNA polymerase III holoenzyme: an asymmetric dimeric replicative complex with leading and lagging strand polymerases.

  2001cited by this paper
• Crystal Structure of the Processivity Clamp Loader Gamma ( ) Complex of E. coli DNA Polymerase III

  2001cited by this paper
• ATP Utilization by Yeast Replication Factor C

  2001cited by this paper
• A simple, high-resolution method for establishing DNA binding affinity and sequence selectivity.

  2001cited by this paper
• Crystal structure of the processivity clamp loader gamma (gamma) complex of E. coli DNA polymerase III.

  2001cited by this paper
• ATP Utilization by Yeast Replication Factor C I. ATP-MEDIATED INTERACTION WITH DNA AND WITH PROLIFERATING CELL NUCLEAR ANTIGEN*

  2001cited by this paper
• A homogeneous method to measure aminoacyl-tRNA synthetase aminoacylation activity using scintillation proximity assay technology.

  2000cited by this paper
• DNA replication in the third domain (of life).

  2000cited by this paper
• In Vitro Antimicrobial Activities of Novel Anilinouracils Which Selectively Inhibit DNA Polymerase III of Gram-Positive Bacteria

  2000cited by this paper
• The DNA Replication Machine of a Gram-positive Organism*

  2000cited by this paper
• A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays

  1999cited by this paper
• Trading places on DNA--a three-point switch underlies primer handoff from primase to the replicative DNA polymerase.

  1999cited by this paper
• Fluorometric Assay for DNA Polymerases and Reverse Transcriptase

  1999cited by this paper
• A robotics-based automated assay for inorganic and organic phosphates.

  1999cited by this paper
• Devoted to the lagging strand—the χ subunit of DNA polymerase III holoenzyme contacts SSB to promote processive elongation and sliding clamp assembly

  1998cited by this paper
• The DNA replication fork in eukaryotic cells.

  1998cited by this paper
• The χψ Subunits of DNA Polymerase III Holoenzyme Bind to Single-stranded DNA-binding Protein (SSB) and Facilitate Replication of an SSB-coated Template*

  1998cited by this paper
• An automated high throughput filtration assay: application to polymerase inhibitor identification.

  1997cited by this paper
• Gram-Positive Bacteria

  1997cited by this paper
• Synthesis and antimicrobial activity of some bis-quinazoline derivatives.

  1997cited by this paper
• Mitochondrial Injury

  1997cited by this paper
• Fluorometric assay for DNA polymerases and reverse transcriptase.

  1996cited by this paper
• Biotin Tagging Deletion Analysis of Domain Limits Involved in Protein-Macromolecular Interactions

  1996cited by this paper
• Identification of the β-binding Domain of the α Subunit of Escherichia coli Polymerase III Holoenzyme*

  1996cited by this paper
• Purification and cloning of a soluble ATP-diphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum).

  1996cited by this paper
• Coupling of a replicative polymerase and helicase: a tau-DnaB interaction mediates rapid replication fork movement.

  1996cited by this paper
• In Vivo Assembly of Overproduced DNA Polymerase III

  1996cited by this paper
• Escherichia coli DNA polymerase III holoenzyme subunits alpha, beta, and gamma directly contact the primer-template.

  1995cited by this paper
• Kinetic analysis of nucleotide incorporation and misincorporation by Klenow fragment of Escherichia coli DNA polymerase I.

  1995cited by this paper
• SB 205952, a novel semisynthetic monic acid analog with at least two modes of action

  1995cited by this paper
• Escherichia coli DNA Polymerase III Holoenzyme Subunits α, β, and γ Directly Contact the Primer-Template (*)

  1995cited by this paper
• Melting of a DNA helix terminus within the active site of a DNA polymerase.

  1994cited by this paper
• DNA polymerase III accessory proteins. I. holA and holB encoding delta and delta'.

  1993cited by this paper
• Identification, isolation, and overexpression of the gene encoding the psi subunit of DNA polymerase III holoenzyme

  1993cited by this paper
• DNA polymerase III accessory proteins. III. holC and holD encoding chi and psi.

  1993cited by this paper
• Compilation, alignment, and phylogenetic relationships of DNA polymerases.

  1993cited by this paper
• Glutamate overcomes the salt inhibition of DNA polymerase III holoenzyme.

  1989cited by this paper
• Proofreading by DNA polymerase III of Escherichia coli depends on cooperative interaction of the polymerase and exonuclease subunits.

  1987cited by this paper
• Chemical characterization and purification of the beta subunit of the DNA polymerase III holoenzyme from an overproducing strain.

  1986cited by this paper
• Size classes of products synthesized processively by two subassemblies of Escherichia coli DNA polymerase III holoenzyme.

  1982cited by this paper
• Size classes of products synthesized processively by DNA polymerase III and DNA polymerase III holoenzyme of Escherichia coli.

  1981cited by this paper
• Purification and characterization of the beta subunit of the DNA polymerase III holoenzyme of Escherichia coli.

  1980cited by this paper
• Cloning of a functional replication origin of phage G4 into the genome of phage M13.

  1979cited by this paper
• A quantitative assay for bacterial RNA polymerases.

  1979cited by this paper
• The RNA primer synthesized by primase to initiate phage G4 DNA replication.

  1978cited by this paper
• A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.

  1969cited by this paper

• Life at the replication fork: A scientific and personal journey

  2024cites this paper
• Inhibition of Replication Fork Formation and Progression: Targeting the Replication Initiation and Primosomal Proteins

  2023cites this paper
• The RecD2 helicase balances RecA activities

  2022cites this paper
• Novel Antibiotics Targeting Bacterial Replicative DNA Polymerases

  2020cites this paper
• DNA Replication, Chemical Biology of

  2018cites this paper
• The Macromolecular Machines that Duplicate the Escherichia coli Chromosome as Targets for Drug Discovery

  2018cites this paper
• Initiation Complex Formation, Mechanism of

  2018cites this paper
• Recent Advances in Helicobacter pylori Replication: Possible Implications in Adaptation to a Pathogenic Lifestyle and Perspectives for Drug Design.

  2017cites this paper
• Optimization of a Luminescence-Based High-Throughput Screening Assay for Detecting Apyrase Activity

  2017cites this paper
• The β2 clamp in the Mycobacterium tuberculosis DNA polymerase III αβ2ε replicase promotes polymerization and reduces exonuclease activity

  2016cites this paper
• Mechanism of Initiation Complex Formation

  2015cites this paper
• Chemical Biology of DNA Replication

  2014cites this paper
• Nucleotide Metabolism and DNA Replication

  2014cites this paper
• Investigating protein-DNA interactions at replication forks by photo-crosslinking

  2013cites this paper
• A structural role for the PHP domain in E. coli DNA polymerase III

  2013cites this paper
• A bacterial toxin inhibits DNA replication elongation through a direct interaction with the β sliding clamp.

  2013cites this paper
• The PriA Replication Restart Protein Blocks Replicase Access Prior to Helicase Assembly and Directs Template Specificity through Its ATPase Activity*

  2012cites this paper
• A small molecule inhibitor of Pot1 binding to telomeric DNA.

  2012cites this paper
• Architecture and Conservation of the Bacterial DNA Replication Machinery, an Underexploited Drug Target

  2012cites this paper
• Bacterial replicases and related polymerases.

  2011cites this paper
• Binding inhibitors of the bacterial sliding clamp by design.

  2011cites this paper
• Breaking the rules: bacteria that use several DNA polymerase IIIs

  2011influential citation
• DNA replicases from a bacterial perspective.

  2011cites this paper
• Small‐molecule Inhibitors of Dna Polymerase Function Weitere Publikationen: 4.4.2) Side‐by‐side Comparison of Sm1 and Sm49 with Reported Inhibitors ...................38 5) Synthesis of 4'‐c‐alkylated‐5‐iodo‐2'‐deoxypyrimidine Nucleosides as Potential Antiviral Drugs and Synthetic Building Blocks ..

  year unknowncites this paper