Use of coherent control for selective two-photon fluorescence microscopy in live organisms.

We demonstrate selective fluorescence We demonstrate selective fluorescence excitation of specific molecular species in live organisms by using coherent control of two-photon excitation. We have acquired quasi-simultaneous images in live fluorescently-labeled Drosophila embryos by rapid switching between appropriate pulse shapes. Linear combinations of these images demonstrate that a high degree of fluorophore selectivity is attainable through phase-shaping. Broadband phase-shaped excitation opens up new possibilities for single-laser, multiplex, in-vivo fluorescence microscopy.

• Publication year

  2006

• Venue

  Optics Express

• Publication date

  2006-01-23

• Fields of study

  Biology, Materials Science, Physics, Engineering, Medicine

• Identifiers
  DOI 10.1364/OPEX.14.000759PMID 19503394
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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