A Proton Pump ATPase with Testis-specific E1-Subunit Isoform Required for Acrosome Acidification*

The vacuolar-type H+-ATPases (V-ATPases) are a family of multimeric proton pumps involved in a wide variety of physiological processes. We have identified two novel mouse genes, Atp6e1 and Atp6e2, encoding testis-specific (E1) and ubiquitous (E2) V-ATPase subunit E isoforms, respectively. TheE1 transcript appears about 3 weeks after birth, corresponding to the start of meiosis, and is expressed specifically in round spermatids in seminiferous tubules. Immunohistochemistry with isoform-specific antibodies revealed that the V-ATPase withE1 and a2 isoforms is located specifically in developing acrosomes of spermatids and acrosomes in mature sperm. In contrast, the E2 isoform was expressed in all tissues examined and present in the perinuclear compartments of spermatocytes. The E1 isoform exhibits 70% identity with theE2, and both isoforms functionally complemented a null mutation of the yeast counterpart VMA4, indicating that they are bona fide V-ATPase subunits. The chimeric enzymes showed slightly lower K m ATP than yeast V-ATPase. Consistent with the temperature-sensitive growth of Δvma4-expressing E1 isoform, vacuolar membrane vesicles exhibited temperature-sensitive coupling between ATP hydrolysis and proton transport. These results suggest thatE1 isoform is essential for energy coupling involved in acidification of acrosome.

• Publication year

  2002

• Venue

  Journal of Biological Chemistry

• Publication date

  2002-05-17

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/JBC.M111567200PMID 11872743
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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