Molecular characterization of exosome-like vesicles from breast cancer cells

BackgroundMembrane vesicles released by neoplastic cells into extracellular medium contain potential of carrying arrays of oncogenic molecules including proteins and microRNAs (miRNA). Extracellular (exosome-like) vesicles play a major role in cell-to-cell communication. Thus, the characterization of proteins and miRNAs of exosome-like vesicles is imperative in clarifying intercellular signaling as well as identifying disease markers.MethodsExosome-like vesicles were isolated using gradient centrifugation from MCF-7 and MDA-MB 231 cultures. Proteomic profiling of vesicles using liquid chromatography-mass spectrometry (LC-MS/MS) revealed different protein profiles of exosome-like vesicles derived from MCF-7 cells (MCF-Exo) than those from MDA-MB 231 cells (MDA-Exo).ResultsThe protein database search has identified 88 proteins in MDA-Exo and 59 proteins from MCF-Exo. Analysis showed that among all, 27 proteins were common between the two exosome-like vesicle types. Additionally, MDA-Exo contains a higher amount of matrix-metalloproteinases, which might be linked to the enhanced metastatic property of MDA-MB 231 cells. In addition, microarray analysis identified several oncogenic miRNA between the two types vesicles.ConclusionsIdentification of the oncogenic factors in exosome-like vesicles is important since such vesicles could convey signals to non-malignant cells and could have an implication in tumor progression and metastasis.

• Publication year

  2014

• Venue

  BMC Cancer

• Publication date

  2014-01-27

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1186/1471-2407-14-44PMID 24468161PMCID 3936808
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The Oncogenic Role of microRNA-130a/301a/454 in Human Colorectal Cancer via Targeting Smad4 Expression

  2013cited by this paper
• The miR-106b-25 cluster targets Smad7, activates TGF-β signaling, and induces EMT and tumor initiating cell characteristics downstream of Six1 in human breast cancer

  2012cited by this paper
• The Oncogenic Role of miR-155 in Breast Cancer

  2012cited by this paper
• Proteomic analysis of exosome-like vesicles derived from breast cancer cells.

  2012cited by this paper
• MicroRNA-301 mediates proliferation and invasion in human breast cancer.

  2011cited by this paper
• Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers

  2011cited by this paper
• MiR-34a Expression Has an Effect for Lower Risk of Metastasis and Associates with Expression Patterns Predicting Clinical Outcome in Breast Cancer

  2011cited by this paper
• Exosome Secretion: Molecular Mechanisms and Roles in Immune Responses

  2011cited by this paper
• Exosomes: extracellular organelles important in intercellular communication.

  2010cited by this paper
• Mammalian microRNAs: experimental evaluation of novel and previously annotated genes.

  2010cited by this paper
• Circulating tumour-derived microvesicles in plasma of gastric cancer patients

  2010cited by this paper
• Selective Release of MicroRNA Species from Normal and Malignant Mammary Epithelial Cells

  2010influential reference
• Circulating microRNAs as Novel Minimally Invasive Biomarkers for Breast Cancer

  2010cited by this paper
• Relevance of circulating tumor cells, extracellular nucleic acids, and exosomes in breast cancer

  2010cited by this paper
• Structural heterogeneity and protein composition of exosome‐like vesicles (prostasomes) in human semen

  2009cited by this paper
• Large-scale proteomics and phosphoproteomics of urinary exosomes.

  2009cited by this paper
• Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).

  2009cited by this paper
• Absolute quantification of microRNAs by using a universal reference.

  2009cited by this paper
• Calmodulin modulates Akt activity in human breast cancer cell lines

  2009cited by this paper
• Exosomes: proteomic insights and diagnostic potential

  2009cited by this paper
• Proteomics of MUC1‐containing lipid rafts from plasma membranes and exosomes of human breast carcinoma cells MCF‐7

  2009cited by this paper
• MicroRNA miR-328 Regulates Zonation Morphogenesis by Targeting CD44 Expression

  2008cited by this paper
• Proteomic profiling of exosomes: Current perspectives

  2008cited by this paper
• Glioblastoma microvesicles transport RNA and protein that promote tumor growth and provide diagnostic biomarkers

  2008cited by this paper
• Exosomal microRNA: a diagnostic marker for lung cancer.

  2008cited by this paper
• Intercellular transfer of the oncogenic receptor EGFRvIII by microvesicles derived from tumour cells

  2008cited by this paper
• Exosomes with Immune Modulatory Features Are Present in Human Breast Milk1

  2007cited by this paper
• Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells

  2007cited by this paper
• Loss of DNA methylation and histone H4 lysine 20 trimethylation in human breast cancer cells is associated with aberrant expression of DNA methyltransferase 1, Suv4-20h2 histone methyltransferase and methyl-binding proteins

  2006cited by this paper
• Association of citrullinated proteins with synovial exosomes.

  2006cited by this paper
• Exosomes: from biogenesis and secretion to biological function.

  2006cited by this paper
• RNA integrity and the effect on the real-time qRT-PCR performance.

  2006cited by this paper
• Transforming Growth Factor β Receptor Type I and Transforming Growth Factor β1 Polymorphisms Are Not Associated with Postmenopausal Breast Cancer

  2006cited by this paper
• Relevance of Breast Cancer Cell Lines as Models for Breast Tumours: An Update

  2004cited by this paper
• Exosomes secreted from monocyte-derived dendritic cells support in vitro naive CD4+ T cell survival through NF-(kappa)B activation.

  2004cited by this paper
• BMC Cancer

  2003cited by this paper
• TM4: a free, open-source system for microarray data management and analysis.

  2003influential reference
• Matrix-degrading proteinases are shed in membrane vesicles by ovarian cancer cells in vivo and in vitro

  1999cited by this paper
• Selective localization of matrix metalloproteinase 9, beta1 integrins, and human lymphocyte antigen class I molecules on membrane vesicles shed by 8701-BC breast carcinoma cells.

  1998cited by this paper
• The amount and proteolytic content of vesicles shed by human cancer cell lines correlates with their in vitro invasiveness.

  1998cited by this paper
• Cluster analysis and display of genome-wide expression patterns.

  1998cited by this paper

• Identification of DYRK1b as a novel regulator of small extracellular vesicle release using a high throughput nanoscale flow cytometry screening platform.

  2025cites this paper
• Exosomal MicroRNA: Diagnostic Potential and Role in Breast Cancer Dissemination

  2025cites this paper
• Exosomal lncRNA MIR210HG derived from hypoxic TAMs promotes the metastasis of triple negative breast cancer.

  2025cites this paper
• The impact of extracellular vesicles on breast cancer metastasis and therapeutics: genetic considerations.

  2025cites this paper
• Human osteosarcoma cell secretome impairs neonatal mouse calvarial osteogenic cells functions and modifies the nanoparticles-derived protein profile.

  2025cites this paper
• Review projecting shikonin as a therapeutic candidate in female carcinomas: a preclinical perspective

  2025cites this paper
• Elevated extracellular vesicular Nm23-H1 subdues the pro-migratory potential of breast cancer cell-derived extracellular vesicles.

  2024cites this paper
• vCPP2319 interacts with metastatic breast cancer extracellular vesicles (EVs) and transposes a human blood-brain barrier model

  2024cites this paper
• Endosomal protein DENND10/FAM45A integrates extracellular vesicle release with cancer cell migration

  2024cites this paper
• Extracellular vesicle proteins as breast cancer biomarkers: Mass spectrometry‐based analysis

  2024cites this paper
• Decoding the secrets of small extracellular vesicle communications: exploring the inhibition of vesicle-associated pathways and interception strategies for cancer treatment.

  2024cites this paper
• Isolation of Extracellular Vesicles Using Formulas to Adapt Centrifugation to Different Centrifuges.

  2024cites this paper
• The role and underlying mechanism of dental pulp stem cell-derived exosomal miR-31 in the treatment of osteoarthritis by targeting mTOR to enhance chondrocyte autophagy levels

  2023cites this paper
• Extracellular vesicles-associated miRNAs in triple-negative breast cancer: From tumor biology to clinical relevance.

  2023cites this paper
• Research trends and hotspots in prostate cancer associated exosome: a bibliometric analysis

  2023cites this paper
• Deciphering the Functional Status of Breast Cancers through the Analysis of Their Extracellular Vesicles

  2023cites this paper
• Development and Characterization of a Gel Formulation Containing Golden Cherry Exosomes (Physalis minima) as a Potential Anti-Photoaging.

  2023cites this paper
• Lysine Acetylome of Breast Cancer-Derived Small Extracellular Vesicles Reveals Specific Acetylation Patterns for Metabolic Enzymes

  2023cites this paper
• Comparative study of size exclusion chromatography for isolation of small extracellular vesicle from cell-conditioned media, plasma, urine, and saliva

  2023cites this paper
• Fibroblasts as Turned Agents in Cancer Progression

  2023cites this paper
• Animal models of bone metastatic prostate cancer

  2023cites this paper
• Exosomes—Nature’s Lipid Nanoparticles, a Rising Star in Drug Delivery and Diagnostics

  2022cites this paper
• Unfolded protein response and angiogenesis in malignancies.

  2022cites this paper
• Protein disulfide isomerase A3 activity promotes extracellular accumulation of proteins relevant to basal breast cancer outcomes in human MDA-MB-A231 breast cancer cells

  2022cites this paper
• The transmission and toxicity of polymer-bound doxorubicin-containing exosomes derived from human adenocarcinoma cells.

  2022cites this paper
• Detection of HPV RNA in Extracellular Vesicles from Neuroendocrine Cervical Cancer Cells

  2022cites this paper
• Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems

  2022cites this paper
• Exosome-driven liquid biopsy for breast cancer: Recent advances in isolation, biomarker identification and detection

  2022cites this paper
• The Chaperone System in Breast Cancer: Roles and Therapeutic Prospects of the Molecular Chaperones Hsp27, Hsp60, Hsp70, and Hsp90

  2022cites this paper
• Exosomal microRNAs in diabetic heart disease

  2022cites this paper
• Aspects of Biological Replication and Evolution Independent of the Central Dogma: Insights from Protein-Free Vesicular Transformations and Protein-Mediated Membrane Remodeling

  2022cites this paper
• Exosomal microRNAs synergistically trigger stromal fibroblasts in breast cancer

  2022cites this paper
• Phosphoproteomic Analysis of Breast Cancer-Derived Small Extracellular Vesicles Reveals Disease-Specific Phosphorylated Enzymes

  2022cites this paper
• A Label-Free Electrical Impedance Spectroscopy for Detection of Clusters of Extracellular Vesicles Based on Their Unique Dielectric Properties

  2022cites this paper
• Applications of Extracellular Vesicles in Triple-Negative Breast Cancer

  2022cites this paper
• Potential Application of Exosomes in Vaccine Development and Delivery

  2022cites this paper
• Molecular insights of metastasis and cancer progression derived using 3D cancer spheroid co-culture in vitro platform.

  2021cites this paper
• Tumor-Derived Exosomal Non-Coding RNAs: The Emerging Mechanisms and Potential Clinical Applications in Breast Cancer

  2021cites this paper
• Exosomes: A Forthcoming Era of Breast Cancer Therapeutics

  2021cites this paper
• Perspective: Why and How Ubiquitously Distributed, Vascular-Associated, Pluripotent Stem Cells in the Adult Body (vaPS Cells) Are the Next Generation of Medicine

  2021cites this paper
• Exosomal lncRNA LINC01711 facilitates metastasis of esophageal squamous cell carcinoma via the miR-326/FSCN1 axis

  2021cites this paper
• Quantitative Nanomechanical Analysis of Small Extracellular Vesicles for Tumor Malignancy Indication

  2021cites this paper
• CD151 enrichment in exosomes of luminal androgen receptor breast cancer cell line contributes to cell invasion.

  2021cites this paper
• Breast adenocarcinoma-derived exosomes lower first-contact de-adhesion strength of adenocarcinoma cells to brain endothelial layer.

  2021cites this paper
• Exosomes in ovarian cancer ascites promote epithelial–mesenchymal transition of ovarian cancer cells by delivery of miR-6780b-5p

  2021cites this paper
• Cancer-derived exosomal miR-7641 promotes breast cancer progression and metastasis

  2021cites this paper
• The characterization of exosomes from fibrosarcoma cell and the useful usage of Dynamic Light Scattering (DLS) for their evaluation

  2021cites this paper
• Engineering of Extracellular Vesicles as Nano Therapy for Breast Cancer

  2021cites this paper
• Solid-phase extraction of exosomes from diverse matrices via a polyester capillary-channeled polymer (C-CP) fiber stationary phase in a spin-down tip format

  2020cites this paper
• Resistive-Pulse Sensing Inside Single Living Cells.

  2020cites this paper
• Breast cancer‐derived exosomes: Tumor progression and therapeutic agents

  2020influential citation
• Exosomes and exosomal RNAs in breast cancer: A status update.

  2020cites this paper
• The role of exosomes in metastasis and progression of melanoma.

  2020cites this paper
• Sex-Specific Differences in Extracellular Vesicle Protein Cargo in Synovial Fluid of Patients with Osteoarthritis

  2020cites this paper
• Cancer Extracellular Vesicles: Next-Generation Diagnostic and Drug Delivery Nanotools

  2020cites this paper
• Conferring receptors on recipient cells with extracellular vesicles for targeted drug delivery

  2020cites this paper
• Extracellular Vesicle-Mediated Purinergic Signaling Contributes to Host Microenvironment Plasticity and Metastasis in Triple Negative Breast Cancer

  2020cites this paper
• Advances in study on exosomes and their applications in kidney diseases.

  2020cites this paper
• The Biology of Exosomes in Breast Cancer Progression: Dissemination, Immune Evasion and Metastatic Colonization

  2020cites this paper
• Extracellular vesicles in infectious diseases caused by protozoan parasites in buffaloes

  2020cites this paper
• Molecular and functional extracellular vesicle analysis using nanopatterned microchips monitors tumor progression and metastasis

  2020cites this paper
• Targeting TRAF3IP2, Compared to Rab27, is More Effective in Suppressing the Development and Metastasis of Breast Cancer

  2020cites this paper
• Tumor-derived extracellular vesicles: reliable tools for Cancer diagnosis and clinical applications

  2019cites this paper
• Breast Tumor Microenvironment Can Transform Naive Mesenchymal Stem Cells into Tumor-Forming Cells in Nude Mice

  2019cites this paper
• 3D cell culture stimulates the secretion of in vivo like extracellular vesicles

  2019cites this paper
• Exosomal Transport of Hepatocyte‐Derived Drug‐Modified Proteins to the Immune System

  2019cites this paper
• [Research progress of exosomes in epithelial-mesenchymal transition].

  2019cites this paper
• Fundamentals of Stem Cells: Why and How Patients' Own Adult Stem Cells Are the Next Generation of Medicine

  2019cites this paper
• Emerging roles of exosomal miRNAs in breast cancer drug resistance

  2019cites this paper
• The Role of Exosomal MicroRNAs in the Tumor Microenvironment of Breast Cancer

  2019cites this paper
• DNA analysis of low- and high-density fractions defines heterogeneous subpopulations of small extracellular vesicles based on their DNA cargo and topology

  2019cites this paper
• Extracellular Vesicles From Osteotropic Breast Cancer Cells Affect Bone Resident Cells

  2019cites this paper
• Exosomes and cancer: From oncogenic roles to therapeutic applications

  2019cites this paper
• Exploring the key communicator role of exosomes in cancer microenvironment through proteomics

  2019cites this paper
• Glioma-associated mesenchymal stem cells have profound effects on brain tumors

  2019cites this paper
• 3D cell culture stimulates the secretion of in vivo like exosomes

  2019cites this paper
• Exosomal transport of hepatocyte-derived drug-modified proteins to the immune system

  2019cites this paper
• Proteome profiling of extracellular vesicles captured with the affinity peptide Vn96: comparison of Laemmli and TRIzol© protein-extraction methods

  2018influential citation
• Tumor‐derived exosomes: Potential biomarker or therapeutic target in breast cancer?

  2018cites this paper
• Annexin A1 May Induce Pancreatic Cancer Progression as a Key Player of Extracellular Vesicles Effects as Evidenced in the In Vitro MIA PaCa-2 Model System

  2018cites this paper
• Extracellular Vesicles and Cancer: Caveat Lector

  2018cites this paper
• Extracellular Vesicles, Ageing, and Therapeutic Interventions

  2018cites this paper
• Large extracellular vesicles carry most of the tumour DNA circulating in prostate cancer patient plasma

  2018cites this paper
• A Ratiometric Fluorescent Bioprobe Based on Carbon Dots and Acridone Derivate for Signal Amplification Detection Exosomal microRNA.

  2018cites this paper
• Roles of Extracellular Vesicles in Metastatic Breast Cancer

  2018cites this paper
• Exosomes in cancer: small vesicular transporters for cancer progression and metastasis, biomarkers in cancer therapeutics

  2018cites this paper
• Secretome within the bone marrow microenvironment: A basis for mesenchymal stem cell treatment and role in cancer dormancy.

  2018cites this paper
• Exosome-Mediated Transfer of Cancer Cell Resistance to Antiestrogen Drugs

  2018cites this paper
• Exosome: Function and Role in Cancer Metastasis and Drug Resistance

  2018cites this paper
• Extracellular NME proteins: a player or a bystander?

  2018cites this paper
• Exosome-Based Cell-Cell Communication in the Tumor Microenvironment

  2018cites this paper
• Exosomal microRNA: a novel biomarker for breast cancer.

  2018cites this paper
• Secretomes reveal several novel proteins as well as TGF-β1 as the top upstream regulator of metastatic process in breast cancer

  2018cites this paper
• Translational models of prostate cancer bone metastasis

  2018cites this paper
• Isolation and analysis of a E0771 breast cancer subline with specific metastatic abilities to the lungs

  2018cites this paper
• Mesenchymal Stem Cell-Derived Exosomes: Immunomodulatory Evaluation in an Antigen-Induced Synovitis Porcine Model

  2017cites this paper
• Post‐cardiac arrest level of free‐plasma DNA and DNA‐histone complexes

  2017cites this paper
• Tumor-derived exosomes : potential biomarker or therapeutic target in breast cancer ? † Running title : exosomes in breast cancer

  2017cites this paper
• Role of MIR-29B-1 and MIR-29A in endocrine-resistant breast cancer.

  2017cites this paper
• Exosome: emerging biomarker in breast cancer

  2017cites this paper