Biosynthesis of (R)‐(+)‐perillyl alcohol by Escherichia coli expressing neryl pyrophosphate synthase

(R)‐(+)‐perillyl alcohol is widely used in agricultural and anticarcinogenic fields. Microbial production of (R)‐(+)‐perillyl alcohol was investigated in this study. We optimized biosynthesis of (R)‐(+)‐perillyl alcohol in Escherichia coli by using neryl pyrophosphate synthase and NADPH regeneration. Engineering neryl pyrophosphate (NPP)‐supplied pathway resulted in a 4‐fold improvement of (R)‐(+)‐perillyl alcohol titer. Subsequently, combined engineering of p‐cymene monooxygenase (CymA) expression and module for NADPH regeneration exhibited a 15.4‐fold increase of titer over the initial strain S02. Finally, 453 mg/L (R)‐(+)‐perillyl alcohol was achieved in fed‐batch fermentation, which is the highest (R)‐(+)‐perillyl alcohol titer in E. coli.

• Publication year

  2022

• Venue

  Engineering in Life Sciences

• Publication date

  2022-02-13

• Fields of study

  Agricultural and Food Sciences, Medicine, Chemistry

• Identifiers
  DOI 10.1002/elsc.202100135PMID 35573132PMCID 9077825
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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