WRKY transcription factors (TFs) play an important role in plant responses to biotic and abiotic stress as well as in plant growth and development. In the present study, bioinformatics methods were used to identify members of the WRKY transcription factor family in the Musa acuminata (DH-Pahang) genome (version 2). A total of 164 MaWRKYs were identified and phylogenetic analysis showed that MaWRKYs could be categorized into three subfamilies. Overall, the 162 MaWRKYs were distributed on 11 chromosomes, and 2 genes were not located on the chromosome. There were 31 collinear genes from segmental duplication and 7 pairs of genes from tandem duplication. RNA-sequencing was used to analyze the expression profiles of MaWRKYs in different fruit development, ripening stages, under various abiotic and biotic stressors. Most of the MaWRKYs showed a variety of expression patterns in the banana fruit development and ripening stages. Some MaWRKYs responded to abiotic stress, such as low temperature, drought, and salt stress. Most differentially expressed MaWRKYs were downregulated during banana’s response to Foc TR4 infection, which plays an important role in physiological regulation to stress. Our findings indicate that MaWRKY21 directly binds to the W-box of the MaICS promoter to decrease MaICS transcription and then reduce the enzyme activity. These studies have improved our understanding of the molecular basis for the development and stress resistance of an important banana variety.
Genome-Wide Analysis of the Banana WRKY Transcription Factor Gene Family Closely Related to Fruit Ripening and Stress
C. Jia,Zhuo Wang,Jingyi Wang,Hongxia Miao,Jian-bin Zhang,Biyu Xu,Juhua Liu,Zhiqiang Jin,Jihong Liu
Published 2022 in Plants
ABSTRACT
PUBLICATION RECORD
- Publication year
2022
- Venue
Plants
- Publication date
2022-02-28
- Fields of study
Biology, Medicine, Environmental Science
- Identifiers
- External record
- Source metadata
Semantic Scholar, PubMed
CITATION MAP
EXTRACTION MAP
CLAIMS
CONCEPTS
- fruit development and ripening stages
The developmental sampling series spanning banana fruit maturation and ripening used for expression profiling.
Aliases: banana fruit stages
- maics promoter
The promoter region upstream of the MaICS gene that was tested for transcription-factor binding.
Aliases: MaICS upstream region
- mawrky21
A specific banana WRKY protein examined for its interaction with the MaICS promoter.
Aliases: banana WRKY21
- mawrky subfamilies
The three phylogenetic groups used to organize the identified banana WRKY proteins.
Aliases: MaWRKY groups
- mawrky transcription factors
Banana WRKY-family proteins encoded in Musa acuminata that were surveyed as candidate transcription factors.
Aliases: banana WRKY transcription factors
- musa acuminata (dh-pahang) genome version 2
The reference banana genome assembly used to identify WRKY genes in this study.
Aliases: DH-Pahang genome v2, Musa acuminata genome version 2
- stress response conditions
The low-temperature, drought, salt, and Foc TR4 challenge conditions used in expression analyses.
Aliases: abiotic and biotic stress treatments, stress treatments
- w-box
A short DNA motif recognized by WRKY transcription factors within target promoters.
REFERENCES
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