Can Platelet-rich Plasma Be Used for Skin Rejuvenation? Evaluation of Effects of Platelet-rich Plasma on Human Dermal Fibroblast

Background Autologous platelet-rich plasma has attracted attention in various medical fields recently, including orthopedic, plastic, and dental surgeries and dermatology for its wound healing ability. Further, it has been used clinically in mesotherapy for skin rejuvenation. Objective In this study, the effects of activated platelet-rich plasma (aPRP) and activated platelet-poor plasma (aPPP) have been investigated on the remodelling of the extracellular matrix, a process that requires activation of dermal fibroblasts, which is essential for rejuvenation of aged skin. Methods Platelet-rich plasma (PRP) and platelet-poor plasma (PPP) were prepared using a double-spin method and then activated with thrombin and calcium chloride. The proliferative effects of aPRP and aPPP were measured by [3H]thymidine incorporation assay, and their effects on matrix protein synthesis were assessed by quantifying levels of procollagen type I carboxy-terminal peptide (PIP) by enzyme-linked immunosorbent assay (ELISA). The production of collagen and matrix metalloproteinases (MMP) was studied by Western blotting and reverse transcriptase-polymerase chain reaction. Results Platelet numbers in PRP increased to 9.4-fold over baseline values. aPRP and aPPP both stimulated cell proliferation, with peak proliferation occurring in cells grown in 5% aPRP. Levels of PIP were highest in cells grown in the presence of 5% aPRP. Additionally, aPRP and aPPP increased the expression of type I collagen, MMP-1 protein, and mRNA in human dermal fibroblasts. Conclusion aPRP and aPPP promote tissue remodelling in aged skin and may be used as adjuvant treatment to lasers for skin rejuvenation in cosmetic dermatology.

• Publication year

  2011

• Venue

  Annals of Dermatology

• Publication date

  2011-11-01

• Fields of study

  Medicine

• Identifiers
  DOI 10.5021/ad.2011.23.4.424PMID 22148008PMCID 3229934
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Face and neck revitalization with platelet-rich plasma (PRP): clinical outcome in a series of 23 consecutively treated patients.

  2010cited by this paper
• Antiwrinkle effect of adipose-derived stem cell: activation of dermal fibroblast by secretory factors.

  2009cited by this paper
• Molecular analysis of aggressive microdermabrasion in photoaged skin.

  2009cited by this paper
• Immunohistochemical expression of matrix metalloproteinases in photodamaged skin by photodynamic therapy

  2009cited by this paper
• Proliferation-Promoting Effect of Platelet-Rich Plasma on Human Adipose–Derived Stem Cells and Human Dermal Fibroblasts

  2008cited by this paper
• Anti-apoptotic role of S100A8 in X-ray irradiated keratinocytes.

  2008cited by this paper
• Effects of platelet rich plasma on colonic anastomosis.

  2008cited by this paper
• Human AB Serum and Thrombin‐Activated Platelet‐Rich Plasma Are Suitable Alternatives to Fetal Calf Serum for the Expansion of Mesenchymal Stem Cells from Adipose Tissue

  2007cited by this paper
• Current opinion about using the platelet-rich gel in orthopaedics and trauma surgery.

  2007cited by this paper
• The in vitro effect of different PRP concentrations on osteoblasts and fibroblasts.

  2006cited by this paper
• Effect of platelet-rich plasma (PRP) concentration on the viability and proliferation of alveolar bone cells: an in vitro study.

  2005cited by this paper
• Review: chemotherapy plus supportive care improves survival and quality of life in advanced or metastatic gastrointestinal cancer more than supportive care alone

  2005cited by this paper
• Platelet-rich plasma: evidence to support its use.

  2004cited by this paper
• Effects of platelet lysates on select bone cell functions.

  2004cited by this paper
• Effects of platelet growth factors on human mesenchymal stem cells and human endothelial cells in vitro.

  2004cited by this paper
• Platelet-rich plasma: evidence to support its use.

  2004cited by this paper
• Effect of Platelet Releasate on Bone Cell Migration and Recruitment In Vitro

  2003cited by this paper
• Reversal of photodamage with topical growth factors: a pilot study

  2003cited by this paper
• Platelets are mitogenic for periosteum‐derived cells

  2003cited by this paper
• Fibroblasts: what's new in cellular biology?

  2003cited by this paper
• Current Applications of Platelet Gels in Facial Plastic Surgery

  2002cited by this paper
• Growth factor levels in the platelet-rich plasma produced by 2 different methods: curasan-type PRP kit versus PCCS PRP system.

  2002cited by this paper
• Effectiveness of platelet releasate for the treatment of diabetic neuropathic foot ulcers.

  2001cited by this paper
• The use of autologous platelet-rich plasma (platelet gel) and autologous platelet-poor plasma (fibrin glue) in cosmetic surgery.

  2001cited by this paper
• Quantification of growth factor levels using a simplified method of platelet-rich plasma gel preparation.

  2000cited by this paper
• Platelet-rich plasma: Growth factor enhancement for bone grafts.

  1998cited by this paper
• Long-term sun exposure alters the collagen of thepapillary dermis

  1996cited by this paper
• Long-term sun exposure alters the collagen of the papillary dermis. Comparison of sun-protected and photoaged skin by northern analysis, immunohistochemical staining, and confocal laser scanning microscopy.

  1996cited by this paper
• Reduced type I and type III procollagens in photodamaged adult human skin.

  1995cited by this paper
• Ultraviolet A irradiation stimulates collagenase production in cultured human fibroblasts.

  1992cited by this paper

• Beyond the clot: how the biomolecular landscape of platelet-rich fibrin directs fibroblast functions

  2026cites this paper
• Exosomes versus Platelet-rich Plasma in Hair Loss Therapy: A Comprehensive Literature Review

  2026cites this paper
• An Autologous, Vascularized and Immunocompetent Tissue Engineered Skin to Highlight Inter‐Individual Variability to Better Understand the Human Wound Healing

  2026cites this paper
• Mitochondria-Targeted Biophysical Priming of Autologous Biologics for Skin Regeneration and Wound Repair

  2026cites this paper
• Platelet-rich plasma enhances anti-apoptotic and paracrine effects of bone marrow mesenchymal stem cells through the PI3K/Akt pathway.

  2026cites this paper
• Effect of Patient Age on Platelet-Rich Plasma (PRP) and Fibrin Treatments for Skin Density and Thickness: A Single-Center Ultrasound Study

  2025cites this paper
• Tecniche adiuvanti di ringiovanimento facciale

  2025cites this paper
• Platelet-Rich Plasma Plus Microneedling for Scar Management: Introduction to an Innovative Technique and Initial Results From 107 Patients

  2025influential citation
• Efficacy of Concentrated Growth Factors in Treating Tympanic Membrane Perforation in Guinea Pigs

  2025cites this paper
• Proficiency of Topical Platelet-Rich Plasma Over Vacuum-Assisted Closure for Chronic Wound Healing: A Clinical Prospective Comparative Study

  2025cites this paper
• Platelet-rich plasma improves caspase-3 of skin graft treated with Tilapia Mozambique bio-dressing

  2025cites this paper
• Is platelet rich plasma a promising treatment for erectile dysfunction? the first 2-year follow up randomized controlled study

  2025cites this paper
• Applications of Platelet-rich Plasma in the Treatment of Dermatological Disorders

  2025influential citation
• Allergic reactions following platelet-rich plasma injections: a rare but relevant concern

  2025cites this paper
• Harnessing Regenerative Science in Aesthetic Surgery: The Biologically Driven Future

  2025cites this paper
• Efficacy of microneedling with platelet-rich plasma versus microneedling with tranexamic acid in melasma – A randomized open-label study

  2025cites this paper
• The Role of Platelet Concentrates and Growth Factors in Facial Rejuvenation: A Systematic Review with Case Series

  2025cites this paper
• A novel approach to non-surgical management of deep second-degree perianal burn in an immobile patient using platelet-rich plasma: A case report

  2025cites this paper
• Multifaceted Roles of Dermal Fibroblasts in Skin Health and Disease.

  2025cites this paper
• Developing a Reproducible Procedure for Optimal Utilization of Platelet‐Rich Plasma (PRP) and Platelet‐Rich Fibrin (PRF) in Aesthetic Treatments: Efficacy Evaluation Using Ultrasound Imaging—A Single‐Center Prospective Open‐Label Randomized Study

  2024cites this paper
• Effects of Blood-Derived Products on Cellular Senescence and Inflammatory Response: A Study on Skin Rejuvenation

  2024cites this paper
• Management of Erectile Dysfunction Using Platelet-Rich Plasma Cavernosal Injections

  2024cites this paper
• Hyperdiluting Calcium Hydroxylapatite With Platelet-Rich Plasma and Hyaluronidase for Improving Neck Laxity and Wrinkle Severity

  2024cites this paper
• Platelet-rich plasma as an adjuvant therapy to fractional ablative carbon dioxide lasers for cutaneous repair: a complementary treatment for atrophic acne scarring

  2024cites this paper
• Platelet-Rich Plasma: Characteristics and Current Review of its Use in Surgery.

  2024cites this paper
• Future Directions About Keloid Scars Based on Pathogenesis and Therapies

  2024cites this paper
• Use of platelet rich plasma for skin rejuvenation

  2024cites this paper
• Evaluating the Efficacy of Combined Platelet‐Rich Plasma and Microneedling for Aesthetic Rejuvenation of the Periorbital Area: A Randomized, Blinded Cohort Study

  2024cites this paper
• Effectiveness of fractional erbium–YAG laser, microneedling, platelet-rich plasma in localized stable vitiligo patients: randomized clinical trial

  2024cites this paper
• Meta-Analysis of Efficacy of Platelet-Rich Plasma Combined with Minoxidil for Androgenetic Alopecia

  2024cites this paper
• USO DE AGREGADOS PLAQUETÁRIOS EM TRATAMENTOS ESTÉTICOS FACIAIS

  2024cites this paper
• Skin boosters: Definitions and varied classifications

  2024cites this paper
• Safety and efficacy evaluation of intracavernosal injection of platelets rich plasma in treatment of vasculogenic erectile dysfunction

  2024cites this paper
• Stem Cells and Regenerative Strategies for Wound Healing: Therapeutic and Clinical Implications

  2024cites this paper
• AGREGADOS PLAQUETÁRIOS NA HARMONIZAÇÃO OROFACIAL

  2024cites this paper
• The History and Current Status of Platelet-Rich Plasma Therapy in Dermatology

  2024cites this paper
• Autolougous platelet concentrates in esthetic medicine

  2024cites this paper
• The Efficacy of the Combination of Microneedling with Jessner’s Plus 35% Trichloroacetic Acid Peel versus the Efficacy of the Combination of Microneedling with Platelet-rich Plasma Therapy for the Treatment of Atrophic Acne Scars: A Comparative Study

  2024cites this paper
• Systematic Review: Platelet-Rich Plasma Use in Facial Rejuvenation

  2023cites this paper
• Comparative study between platelet-rich plasma (PRP) and mechanically emulsified fat grafts in management of chronic wounds.

  2023cites this paper
• Comparison of the effects of 2 frequencies of application of photobiomodulation on facial rejuvenation: Controlled, randomized, and double-blind clinical trial

  2023cites this paper
• Platelet-Rich Plasma (PRP) in Dermatology: Cellular and Molecular Mechanisms of Action

  2023cites this paper
• Effect of homologous platelet rich fibrin matrix and injectable platelet rich fibrin on full thickness skin autograft healing in dogs

  2023cites this paper
• Platelet-rich Plasma Stimulates Collagen Type I Synthesis in the Human Skin: A Placebo-controlledin vivo Study.

  2023cites this paper
• Recent progress and clinical applications of advanced biomaterials in cosmetic surgery

  2023cites this paper
• Platelet‐rich plasma injection versus combined fractional carbon dioxide laser with platelet‐rich plasma in treatment of vitiligo: A comparative study

  2023cites this paper
• Recent advances in platelet-rich plasma and its derivatives: therapeutic agents for tissue engineering and regenerative medicine

  2023cites this paper
• A bibliometric analysis of trends in the application of platelet‐rich plasma in cosmetics research between 2001 and 2022

  2023cites this paper
• Does Platelet-Rich Plasma Promote Facial Rejuvenation? Revising the Latest Evidence in a Narrative Review

  2023cites this paper
• Alopecia Areata: Current Treatments and New Directions

  2023cites this paper
• A Pilot, Single-Blind, Randomized Controlled Study Evaluating the Use of Platelet-Rich Plasma for Hand Skin Rejuvenation

  2023cites this paper
• Understanding skin aging: Exploring anti-aging modalities

  2023cites this paper
• Efficacy of photo‐thermal‐bioactivated platelet‐rich plasma for skin biostimulation in patients not eligible for other medical‐aesthetic treatment: A pilot study

  2023cites this paper
• Impact of autologous platelet concentrates on wound area reduction: A meta‐analysis of randomized controlled trials

  2023cites this paper
• Platelet-rich plasma in the pathologic processes of tendinopathy: a review of basic science studies

  2023cites this paper
• Innovations in Skin and Soft Tissue Aging—A Systematic Literature Review and Market Analysis of Therapeutics and Associated Outcomes

  2023cites this paper
• Application of platelet-rich plasma (PRP) in lips rejuvenation

  2023cites this paper
• Recurrence rates in the treatment of keloids and hypertrophic scars with intralesional triamcinolone combined with other intralesional agents

  2023cites this paper
• Dermal Fibroblasts as the Main Target for Skin Anti-Age Correction Using a Combination of Regenerative Medicine Methods

  2023cites this paper
• Platelet-rich plasma in surgical practice: a review

  2023cites this paper
• Progress in the clinical treatment of keloids

  2023cites this paper
• Morphologic and molecular biologic analyses of the skin rejuvenation effect of the fractional 1064‐nm picosecond laser: An animal study

  2022cites this paper
• A comparative study of efficacy of fractional carbon dioxide laser with and without platelet rich plasma in treatment of facial acne scars

  2022cites this paper
• Platelet-Rich Plasma: Applications in Cosmetic Dermatology

  2022cites this paper
• Autologous platelet concentrates for facial rejuvenation

  2022cites this paper
• Facial rejuvenation by microneedling with irradiated amniotic collagen matrix compared to platelet rich plasma

  2022cites this paper
• Exploration of proper heating protocol for injectable horizontal platelet-rich fibrin gel

  2022cites this paper
• Preparation and Utility of Platelet-Rich Plasma (PRP) for Facial Aging: A Comprehensive Review

  2022cites this paper
• Current approaches for the regeneration and reconstruction of ocular surface in dry eye

  2022cites this paper
• Volumetric Soft Tissue Changes After Using Injectable Platelet-Rich Fibrin (I-PRF) Versus Subepithelial Connective Tissue Graft in Interdental Papillae Defects: A Randomized Controlled Clinical Study

  2022cites this paper
• Platelet Rich Plasma and Its Use in Hair Regrowth: A Review

  2022cites this paper
• Regenerative Medicine Therapy in Malaysia: An Update

  2022cites this paper
• On the issue of obtaining platelet-rich plasma

  2022cites this paper
• Comparison of clinical results of oral tranexamic acid and platelet rich plasma therapies in melasma treatment

  2022cites this paper
• Effect of platelet-rich plasma on skin quality in patients with involution of the neck area

  2022cites this paper
• Nuclear medicine in the management of superficial skin abnormalities and institutional experience.

  2022cites this paper
• Platelet-rich plasma: how safe is it, and can it cause irreversible blindness?

  2022cites this paper
• The Growing Role for Platelet Rich Plasma in Cosmetic Dermatology

  2022cites this paper
• Nonoperative Facial Rejuvenation

  2022cites this paper
• Proficiency of topical platelet-rich plasma with vacuum-assisted closure over platelet-rich plasma alone in diabetic foot ulcers – A clinical, prospective, comparative study

  2022cites this paper
• Role of Platelet-Rich Plasma Therapy as an Adjuvant in Treatment of Melasma

  2022cites this paper
• Use of platelet-rich fibrin a backbone in facial plastic surgery and wound healing: A review of literature

  2022cites this paper
• Comparative histological and immunohistochemical study on the effect of platelet rich plasma/minoxidil, alone or in combination, on hair growth in a rat model of androgenic alopecia.

  2022cites this paper
• TRANSDERMAL THERAPEUTIC SYSTEMS–AN INNOVATIVE METHOD TO PREVENT THE FORMATION OF KELOIDS

  2022cites this paper
• Current regenerative medicine-based approaches for skin regeneration: A review of literature and a report on clinical applications in Japan

  2022cites this paper
• Basic Science of PRP

  2021cites this paper
• Microneedling with injectable platelet-rich fibrin for facial rejuvenation

  2021cites this paper
• Role of Growth Factors-Rich Plasma, Gel and Membrane in Dermal Wound Healing and Injured Tissue Restoration and Regeneration

  2021cites this paper
• Platelet-Rich Plasma in Melasma—A Systematic Review

  2021cites this paper
• A personalized nutrition plan based on genetic profile improves outcomes of facial regeneration with Platelet-Rich Fibrin liquid matrices

  2021cites this paper
• Current Trends in the Development of Wound Dressings, Biomaterials and Devices

  2021cites this paper
• Platelet-rich Plasma and Its Application in Clinical Trial

  2021cites this paper
• Tranexamic acid and platelet rich plasma in the treatment of melasma: efficacy and safety

  2021cites this paper
• Co-administration of platelet-rich plasma and small intestinal submucosa is more beneficial than their individual use in promoting acute skin wound healing.

  2021cites this paper
• The evidence behind platelet-rich plasma as a hair loss treatment

  2021cites this paper
• Utility of platelet-rich plasma in aesthetics.

  2021cites this paper
• Efficacy and Safety of Platelet-Rich Plasma in Melasma: A Systematic Review and Meta-Analysis

  2021cites this paper
• Phenol Croton Oil Peels

  2021cites this paper
• Smoking status and the baseline international index of erectile function score can predict satisfactory response to platelet‐rich plasma in patients with erectile dysfunction: A prospective pilot study

  2021cites this paper
• Efficacy and safety of plasma gel versus platelet-rich plasma in periorbital rejuvenation: a comparative split-face clinical and Antera 3D camera study

  2021cites this paper