Non-ionic hybrid detergents for protein delipidation.

Non-ionic detergents are important tools for the investigation of interactions between membrane proteins and lipid membranes. Recent studies led to the question whether the ability to capture protein-lipid interactions depends on the properties of detergents or their concentration in purification buffers. To address this question, we present the synthesis of an asymmetric, hybrid detergent that combines the head groups of detergents with opposing delipidating properties. We discuss detergent properties and protein purification outcomes to reveal whether the properties of detergent micelles or the detergent concentration in purification buffers drive membrane protein delipidation. We anticipate that our findings will enable the development of rationally design detergents for future applications in membrane protein research.

• Publication year

  2022

• Venue

  Biochimica et Biophysica Acta - Biomembranes

• Publication date

  2022-05-01

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1016/j.bbamem.2022.183958PMID 35551920
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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