Developmental expression of murine extra-embryonic endodermal cytoskeletal proteins.

Murine extra-embryonic endodermal cell lines derived from either teratocarcinomas or mouse embryos contain a cytoskeletal protein (Endo A) of Mr = 55,000. Endo A was immunoprecipitated from [35S]methionine-labeled lysates of three parietal endodermal cell lines, A presumptive visceral endodermal cell line, and a fetal hepatoma cell line, but not from fibroblasts, myoblasts, erythroleukemic cells, neuroblastoma cells, keratinocytes, or embryonal carcinoma cells. Embryonal carcinoma cells induced to differentiate by exposure to retinoic acid synthesized increased amounts of Endo A approximately 48 h after exposure to the inducer. Two-dimensional gel analysis of immunoprecipitated samples confirmed that Endo A is distinct from vimentin and murine keratinocyte proteins recognized by two different keratin antisera. Comparison by two-dimensional gel electrophoresis of immunoprecipitated Endo A labeled with either [35S]methionine or [32P]orthophosphate indicated that the multiple forms of Endo A resolved by isoelectric focusing were due, at least in part, to phosphorylation. Serine was identified as the phosphorylated amino acid. Endo A was the only major antigenic protein found in a parietal endodermal cell line which was recognized by a monoclonal antibody prepared by other investigators against trophoblast cytoskeletons. The results indicate that Endo A, like the previously described Endo B protein, is distinct from other cytoskeletal proteins and will be useful as a marker of the differentiation of murine embryonal carcinoma cells to extra-embryonic endoderm.

• Publication year

  1982

• Venue

  Journal of Biological Chemistry

• Publication date

  1982-04-10

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/s0021-9258(18)34793-8PMID 6174520
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Reactivity of monoclonal antibodies against intermediate filament proteins during embryonic development.

  1981cited by this paper
• Identification and immunoprecipitation of cytoskeletal proteins from murine extra-embryonic endodermal cells.

  1981cited by this paper
• Comparison of the proteins of two immunologically distinct intermediate-sized filaments by amino acid sequence analysis: desmin and vimentin.

  1981cited by this paper
• Intermediate filaments as mechanical integrators of cellular space

  1980cited by this paper
• The nuclear envelope lamina is reversibly depolymerized during mitosis.

  1980cited by this paper
• Intermediate filaments of baby hamster kidney (BHK-21) cells and bovine epidermal keratinocytes have similar ultrastructures and subunit domain structures.

  1980cited by this paper
• Demonstration of structural polymorphism among HLA-DR light chains by two-dimensional gel electrophoresis

  1980cited by this paper
• Hormonal induction of differentiation in teratocarcinoma stem cells: generation of parietal endoderm by retinoic acid and dibutyryl cAMP.

  1980cited by this paper
• Transforming gene product of Rous sarcoma virus phosphorylates tyrosine

  1980cited by this paper
• Monoclonal antibodies against trophectoderm-specific markers during mouse blastocyst formation.

  1980cited by this paper
• Studies on the effect of retinoids on the differentiation of teratocarcinoma stem cells in vitro and in vivo.

  1980cited by this paper
• Identification of murine extra-embryonic endodermal cells by reaction with teratocarcinoma basement membrane antiserum.

  1980cited by this paper
• Antibodies as probes of cellular differentiation and cytoskeletal organization in the mouse blastocyst.

  1980cited by this paper
• Identification of noncollagenous basement membrane glycopolypeptides synthesized by mouse parietal entoderm and an entodermal cell line.

  1980cited by this paper
• High molecular weight extracellular proteins synthesized by endoderm cells derived from mouse teratocarcinoma cells and normal extraembryonic membranes.

  1980cited by this paper
• H1 histone and nucleosome repeat length alterations associated with the in vitro differentiation of murine embryonal carcinoma cells to extra-embryonic endoderm.

  1980cited by this paper
• The localization and synthesis of some collagen types in developing mouse embryos.

  1979cited by this paper
• Stimulation of differentiation of several murine embryonal carcinoma cell lines by retinoic acid.

  1979cited by this paper
• Properties of a basement membrane-related glycoprotein synthesized in culture by a mouse embryonal carcinoma-derived cell line.

  1979cited by this paper
• Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate.

  1979cited by this paper
• The induction of antigenic changes in a teratocarcinoma stem cell line (F9) by retinoic acid.

  1979cited by this paper
• Different intermediate-sized filaments distinguished by immunofluorescence microscopy.

  1978cited by this paper
• Antibody to prekeratin. Decoration of tonofilament like arrays in various cells of epithelial character.

  1978cited by this paper
• Stimulation of the clonal growth and differentiation of feeder layer dependent mouse embryonal carcinoma cells by beta-mercaptoethanol.

  1978cited by this paper
• An electrofocusing system for the analysis of proteins and their genetic variants.

  1978cited by this paper
• Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1).

  1978cited by this paper
• The induction of differentiation in teratocarcinoma stem cells by retinoic acid.

  1978cited by this paper
• Keratin filaments of cultured human epidermal cells. Formation of intermolecular disulfide bonds during terminal differentiation.

  1978cited by this paper
• 10 nm filaments in normal and transformed cells.

  1978cited by this paper
• Immunocytochemical localization of the major polypeptides of the nuclear pore complex-lamina fraction. Interphase and mitotic distribution

  1978cited by this paper
• Morphological and biochemical observations on cells derived from the in vitro differentiation of the embryonal carcinoma cell line PCC4-F.

  1977cited by this paper
• Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

  1977cited by this paper
• Teratocarcinoma differentiation: plasminogen activator activity associated with embryoid body formation.

  1977cited by this paper
• Clonal lines of teratocarcinoma cells in vitro: Differentiation and cytogenetic characteristics

  1976cited by this paper
• Plasminogen activator in early embryogenesis: enzyme production by trophoblast and parietal endoderm.

  1976cited by this paper
• An octamer of histones in chromatin and free in solution.

  1975cited by this paper
• Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro.

  1975cited by this paper
• Neoplastic differentiation: Characteristics of cell lines derived from a murine teratocarcinoma

  1974cited by this paper
• Removal of proteases from DNase I by chromatography over agarose with covalently attached lima bean protease inhibitor.

  1974cited by this paper
• Alkaline phosphatase activity in mouse teratoma.

  1973cited by this paper
• Fluorescent monitoring of SDS gel electrophoresis.

  1971cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper
• RETENTION OF MULTIPLE DEVELOPMENTAL POTENTIALITIES BY CELLS OF A MOUSE TESTICULAR TERATOCARCINOMA DURING PROLONGED CULTURE in vitro AND THEIR EXTINCTION UPON HYBRIDIZATION WITH CELLS OF PERMANENT LINES.

  1967cited by this paper

• Phosphorylation and Reorganization of Keratin Networks: Implications for Carcinogenesis and Epithelial Mesenchymal Transition

  2015cites this paper
• Keratins in colorectal epithelial function and disease

  2012cites this paper
• Intermediate filaments: a historical perspective.

  2007influential citation
• TRIM28 mediates primer binding site-targeted silencing of murine leukemia virus in embryonic cells.

  2007cites this paper
• Type II keratins precede type I keratins during early embryonic development.

  2005cites this paper
• Concerted gene duplications in the two keratin gene families

  2005cites this paper
• The bHLH protein MyoR inhibits the differentiation of early embryonic endoderm.

  2004cites this paper
• Expression of the nidogen-binding site of the laminin γ1 chain disturbs basement membrane formation and maintenance in F9 embryoid bodies

  2003cites this paper
• Revised MBC # 01-12-0591 Type-II keratins are phosphorylated on a unique motif during stress and mitosis in tissues and cultured cells

  2002cites this paper
• Type II keratins are phosphorylated on a unique motif during stress and mitosis in tissues and cultured cells.

  2002cites this paper
• Phosphorylation of cytokeratin 8 and 18 in human vascular smooth muscle cells of atherosclerotic lesions and umbilical cord vessels

  2001cites this paper
• The significance of the laminin/nidogen-1 interaction for basement membrane formation and stability in embryoid bodies

  2001cites this paper
• DNA microarray analyses of genes regulated during the differentiation of embryonic stem cells

  2000cites this paper
• Characterization of the Major Physiologic Phosphorylation Site of Human Keratin 19 and Its Role in Filament Organization*

  1999cites this paper
• Pervanadate-mediated tyrosine phosphorylation of keratins 8 and 19 via a p38 mitogen-activated protein kinase-dependent pathway.

  1999cites this paper
• Desmoplakin Is Required Early in Development for Assembly of Desmosomes and Cytoskeletal Linkage

  1998influential citation
• Caspase Cleavage of Keratin 18 and Reorganization of Intermediate Filaments during Epithelial Cell Apoptosis

  1997cites this paper
• Methylation of an ETS site in the intron enhancer of the keratin 18 gene participates in tissue-specific repression

  1997cites this paper
• Phosphorylation of Human Keratin 8 in Vivo at Conserved Head Domain Serine 23 and at Epidermal Growth Factor-stimulated Tail Domain Serine 431*

  1997cites this paper
• Two‐dimensional gel analysis of glandular keratin intermediate filament phosphorylation

  1996cites this paper
• Oncogenic regulation and function of keratins 8 and 18

  1996cites this paper
• 14-3-3 proteins associate with phosphorylated simple epithelial keratins during cell cycle progression and act as a solubility cofactor

  1996cites this paper
• Implications of intermediate filament protein phosphorylation

  1996cites this paper
• Constitutive expression of fibroblast growth factor-4 does not alter the growth or the differentiation of embryonal carcinoma cells.

  1996cites this paper
• Association of vimentin intermediate filaments with the centrosome.

  1995cites this paper
• Targeted disruption of vinculin genes in F9 and embryonic stem cells changes cell morphology, adhesion, and locomotion.

  1995cites this paper
• Retinoic acid provokes metaplasia of epithelium formed in vitro by adult human epidermal keratinocytes.

  1995cites this paper
• Structural and biological consequences of increased vimentin expression in simple epithelial cell types.

  1995cites this paper
• What can be learned from intermediate filament gene regulation in the mouse embryo.

  1995cites this paper
• Use of avian cytokines in mammalian embryonic stem cell culture.

  1994cites this paper
• Fate of a headless vimentin protein in stable cell cultures: soluble and cytoskeletal forms.

  1994cites this paper
• Radiation-induced apoptosis in F9 teratocarcinoma cells.

  1994cites this paper
• cis regulation of the keratin 18 gene in transgenic mice

  1993cites this paper
• cisRegulation oftheKeratin 18GeneinTransgenic Mice

  1993cites this paper
• Modifiers of radiation-induced apoptosis.

  1993cites this paper
• Identification of differentiation-dependent DNase I-hypersensitive sites in the mouse EndoA gene.

  1993cites this paper
• A comparative-analysis of the activity of retinoic Acid and cyclic-amp in the induction of differentiation of embryonal carcinoma-cells.

  1993cites this paper
• Expression patterns of vHNF1 and HNF1 homeoproteins in early postimplantation embryos suggest distinct and sequential developmental roles.

  1992cites this paper
• Distribution of desmosomal proteins in F9 embryonal carcinoma cells and epithelial cell derivatives.

  1992cites this paper
• JunB does not inhibit the induction of c‐Jun during the retinoic acid induced differentiation of F9 cells

  1992cites this paper
• Negative regulation of the major histocompatibility complex class I promoter in embryonal carcinoma cells.

  1991cites this paper
• The promoter of the endo A cytokeratin gene is activated by a 3' downstream enhancer.

  1991cites this paper
• Polarized and functional epithelia can form after the targeted inactivation of both mouse keratin 8 alleles

  1991cites this paper
• Gene expression of cytokeratin endo A and endo B during embryogenesis and in adult tissues of mouse.

  1991cites this paper
• XK endo B is preferentially expressed in several induced embryonic tissues during the development of Xenopus laevis.

  1990cites this paper
• Activation of an intron enhancer within the keratin 18 gene by expression of c-fos and c-jun in undifferentiated F9 embryonal carcinoma cells.

  1990cites this paper
• Differential regulation of keratin 8 and 18 messenger RNAs in differentiating F9 cells.

  1990cites this paper
• Differential keratin gene expression during the differentiation of the cement gland of Xenopus laevis.

  1990cites this paper
• Characteristics of embryonic stem cell differentiation: a comparison with two embryonal carcinoma cell lines.

  1990cites this paper
• Epithelial-specific keratin gene expression: identification of a 300 base-pair controlling segment.

  1990cites this paper
• Glycoconjugates and cell surface interactions in pre- and peri-implantation mammalian embryonic development.

  1990cites this paper
• Cytokeratin gene expression in hepatoma hybrid cells: Evidence for regulation incis

  1989cites this paper
• Mechanism of Blastocyst Formation of the Mouse Embryo

  1989cites this paper
• Cytokeratin filaments are present in golden hamster oocytes and early embryos.

  1989cites this paper
• Posttranslational regulation of keratins: degradation of mouse and human keratins 18 and 8

  1989cites this paper
• Exogenous c-myc gene overexpression interferes with early events in F9 cell differentiation.

  1989cites this paper
• Complete sequence of the mouse type-II keratin EndoA: its amino-terminal region resembles mitochondrial signal peptides.

  1988cites this paper
• Sequence analysis of murine cytokeratin endo A (no. 8) cDNA. Evidence for mRNA species initiated upstream of the normal 5' end in PCC4 cells.

  1988cites this paper
• Xenopus endo B is a keratin preferentially expressed in the embryonic notochord.

  1988cites this paper
• Evolutionary Trees of Intermediate Filament Proteins

  1988cites this paper
• Identification of the gene coding for the Endo B murine cytokeratin and its methylated, stable inactive state in mouse nonepithelial cells.

  1988cites this paper
• Cloning of the human keratin 18 gene and its expression in nonepithelial mouse cells

  1988cites this paper
• Disruption of the cytokeratin filament network in the preimplantation mouse embryo.

  1988cites this paper
• Embryonic expression of the human 40-kD keratin: evidence from a processed pseudogene sequence.

  1988cites this paper
• Suppression of endo B cytokeratin by its antisense RNA inhibits the normal coexpression of endo A cytokeratin.

  1987cites this paper
• Preparation and properties of monoclonal and polyclonal antibodies to mouse epidermal growth factor (EGF) receptors: evidence for cryptic EGF receptors in embryonal carcinoma cells.

  1987cites this paper
• Differential distribution of cytokeratins after microinjection of anti-cytokeratin monoclonal antibodies.

  1987cites this paper
• Organ colonization pattern of retinoic acid-treated and -untreated mouse embryonal carcinoma F9 cells.

  1987cites this paper
• An adhesion-defective variant of F9 embryonal carcinoma cells fails to differentiate into visceral endoderm.

  1987cites this paper
• Lamellar cells of sensory receptors and perineural cells of nerve endings of pig skin contain cytokeratins

  1987cites this paper
• Expression of new proteins of the intermediate filament protein family in differentiating F9 embryonal carcinoma cell cytoskeleton.

  1987cites this paper
• A labile inhibitor blocks endo A gene transcription in murine undifferentiated embryonal carcinoma cells.

  1987cites this paper
• Isolation of a clone of F9 teratocarcinoma cells “naturally” resistant to G418

  1987cites this paper
• Characterization of intermediate filaments expressed by Ewing tumor cell lines.

  1987cites this paper
• Cloning of cDNA and amino acid sequence of a cytokeratin expressed in oocytes of Xenopus laevis.

  1986cites this paper
• Murine endodermal cytokeratins Endo A and Endo B are localized in the same intermediate filament.

  1986cites this paper
• Spontaneous differentiation of murine teratocarcinoma stem cells at low temperature

  1986cites this paper
• Mesenchymal-epithelial conversions induced by 5-azacytidine: appearance of cytokeratin Endo-A messenger RNA.

  1986cites this paper
• Comparison of mouse and human keratin 18: A component of intermediate filaments expressed prior to implantation

  1986cites this paper
• Evidence for the existence of an early common biochemical pathway in the differentiation of F9 cells into visceral or parietal endoderm: modulation by cyclic AMP.

  1986cites this paper
• Cytokeratin expression in simple epithelia. III. Detection of mRNAs encoding human cytokeratins nos. 8 and 18 in normal and tumor cells by hybridization with cDNA sequences in vitro and in situ.

  1986cites this paper
• Modulation of protein biosynthesis during early stages of differentiation in retinoic acid treated F9 teratocarcinoma cells.

  1986cites this paper
• Studies of developmental abnormalities at the molecular level of mouse embryos homozygous for the t12 lethal mutation.

  1986cites this paper
• Comparison of mouse and human keratin 18: a component of intermediate filaments expressed prior to implantation.

  1986cites this paper
• Cytokeratin expression in simple epithelia. I. Identification of mRNA coding for human cytokeratin no. 18 by a cDNA clone.

  1986cites this paper
• The immunological approach to the study of preimplantation mammalian development.

  1986cites this paper
• Intermediate filaments of the midgestation rat trophoblast giant cell.

  1986cites this paper
• Manipulation of Mammalian Development

  1986cites this paper
• Molecular cloning and characterization of the Endo B cytokeratin expressed in preimplantation mouse embryos.

  1986cites this paper
• Immunochemistry of Human Teratocarcinoma Stem Cells

  1985cites this paper
• Co-expression of specific acid and basic cytokeratins in teratocarcinoma-derived fibroblasts treated with 5-azacytidine.

  1985cites this paper
• One gene and one pseudogene for the cytokeratin endo A.

  1985cites this paper
• cDNA Cloning of an Extraembryonic Endodermal Intermediate Filament Protein a

  1985cites this paper
• Intermediate Filament Proteins of Mid‐Gestation Trophoblast Giant Cells Are Simple Epithelial Cytokeratins a

  1985cites this paper
• Modulation of specific protein expression in teratocarcinoma cell aggregates by antibodies affecting cell-cell interactions.

  1985cites this paper
• Roles of extracellular matrix components in differentiating teratocarcinoma cells.

  1985cites this paper
• Intermediate Filaments

  1985cites this paper
• Merkel cells express desmosomal proteins and cytokeratins.

  1985cites this paper
• Expression of the cytokeratin endo A gene during early mouse embryogenesis.

  1985cites this paper
• Preimplantation mouse embryos and liver express the same type I keratin gene product.

  1985cites this paper