Comprehensive analysis of metabolome and transcriptome reveals the mechanism of color formation in different leave of Loropetalum Chinense var. Rubrum

Background Loropetalum chinense var. rubrum (L. chinense var. rubrum) is a precious, coloured-leaf native ornamental plant in the Hunan Province. We found an L. chinense var. rubrum tree with three different leaf colours: GL (green leaf), ML (mosaic leaf), and PL (purple leaf). The mechanism of leaf coloration in this plant is still unclear. Therefore, this study aimed to identify the metabolites and genes involved in determining the colour composition of L. chinense var. rubrum leaves, using phenotypic/anatomic observations, pigment content detection, and comparative metabolomics and transcriptomics. Results We observed that the mesophyll cells in PL were purple, while those in GL were green and those in ML were a mix of purple-green. The contents of chlorophyll a, b, carotenoids, and total chlorophyll in PL and ML were significantly lower than those in GL. While the anthocyanin content in PL and ML was significantly higher than that in GL. The metabolomics results showed the differences in the content of cyanidin 3- O -glucoside, delphinidin 3- O -glucoside, cyanidin 3,5- O -diglucoside, pelargonidin, and petunidin 3,5-diglucoside in ML, GL, and PL were significant. Considering that the change trend of anthocyanin content change was consistent with the leaf colour difference, we speculated that these compounds might influence the colour of L. chinense var. rubrum leaves. Using transcriptomics, we finally identified nine differentially expressed structural genes (one ANR ( ANR1217 ); four CYP75A s ( CYP75A 1815, CYP75A 2846, CYP75A 2909, and CYP75A 1716); four UFGT s ( UFGT 1876, UFGT 1649, UFGT 1839, and UFGT 3273) and nine transcription factors (two MYB s ( MYB 1057 and MYB 1211), one MADS-box ( MADS 1235), two AP2-likes ( AP2-like 1779 and AP2-like2 234), one bZIP ( bZIP 3720), two WD 40s ( WD 2173 and WD 1867) and one bHLH ( bHLH 1631) that might be related to flavonoid biosynthesis and then impacted the appearance of colour in L. chinense var. rubrum leaves. Conclusion This study revealed potential molecular mechanisms associated with leaf coloration in L. chinense var. rubrum by analyzing differential metabolites and genes related to the anthocyanin biosynthesis pathway. It also provided a reference for research on leaf colour variation in other ornamental plants.

• Publication year

  2023

• Venue

  BMC Plant Biology

• Publication date

  2023-03-08

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1186/s12870-023-04143-9PMID 36882694PMCID 9993627
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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