Cloning and expression of Treponema pallidum (Nichols) antigen genes in Escherichia coli

No abstract is available for this paper.

• Publication year

  1983

• Venue

  Infection and Immunity

• Publication date

  1983-11-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1128/iai.42.2.435-445.1983PMID 6358023PMCID PMC264449
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Enzyme-linked immunosorbent assay for the detection of serum antibody to outer membrane proteins of Treponema pallidum.

  1983cited by this paper
• Murine monoclonal antibodies specific for virulent Treponema pallidum (Nichols)

  1982cited by this paper
• Humoral immune response in human syphilis to polypeptides of Treponema pallidum.

  1982cited by this paper
• Identification of Treponema pallidum antigens: comparison with a nonpathogenic treponeme.

  1982cited by this paper
• Further studies on replication of virulent Treponema pallidum in tissue cultures of Sf1Ep cells

  1982cited by this paper
• In vitro cultivation of Treponema pallidum: independent confirmation

  1982cited by this paper
• Expression of Treponema pallidum antigens in Escherichia coli.

  1982cited by this paper
• Expression of Treponema pallidum antigens in Escherichia coli K-12

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  1981cited by this paper
• Cultivation of Virulent Treponema pallidum in Tissue Culture

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• Transformation of E. coli using homopolymer-linked plasmid chimeras.

  1981cited by this paper
• Plasmid DNA in Treponema pallidum (Nichols): potential for antibiotic resistance by syphilis bacteria.

  1981cited by this paper
• Analysis of serum IgG against Treponema pallidum protein antigens in experimentally infected rabbits.

  1981cited by this paper
• Cloning and Expression of Treponema pallidum Protein Antigens in Escherichia coli

  1981cited by this paper
• Rapid and simple removal of contaminating RNA from plasmid DNA without the use of RNase.

  1981cited by this paper
• Detection of antibody-accessible proteins on the cell surface of Haemophilus influenzae type b

  1981cited by this paper
• Eukaryotic signal sequence transports insulin antigen in Escherichia coli.

  1980cited by this paper
• Molecular characterization of receptor binding proteins and immunogens of virulent Treponema pallidum

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• Surface characterization of virulent Treponema pallidum.

  1980cited by this paper
• On the cloning of eukaryotic total poly(A)-RNA populations in Escherichia coli.

  1980cited by this paper
• Increased amplification of pBR322 plasmid deoxyribonucleic acid in Escherichia coli K-12 strains RR1 and chi1776 grown in the presence of high concentrations of nucleoside

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• Experimental syphilis in the rabbit: passive transfer of immunity with immunoglobulin G from immune serum.

  1979cited by this paper
• Relationship of Treponema pallidum to Acidic Mucopolysaccharides

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• Simple method for identification of plasmid-coded proteins

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• DNA-directed in vitro synthesis of beta-galactosidase. Purification and characterization of stimulatory factors in an ascites extract.

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• A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

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• Genetics of Treponema: relationship between Treponema pallidum and five cultivable treponemes

  1978cited by this paper
• Mucopolysaccharide Material Resulting from the Interaction of Treponema pallidum (Nichols Strain) with Cultured Mammalian Cells

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  1978cited by this paper
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  1976cited by this paper
• A colony bank containing synthetic CoI EI hybrid plasmids representative of the entire E. coli genome

  1976cited by this paper
• Demonstration of extracellular material at the surface of pathogenic T. pallidum cells.

  1976cited by this paper
• Purification and properties of a soluble factor required for the deoxyribonucleic acid-directed in vitro synthesis of beta-galactosidase.

  1975cited by this paper
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  1975cited by this paper
• Humoral immunity in experimental syphilis. I. The demonstration of resistance conferred by passive immunization.

  1975cited by this paper
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  1973cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

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• Immunogenic properties of the protein component of Treponema pallidum.

  1969cited by this paper
• Immunity in Experimental Syphilis IV. Serological Reactivity of Antigens Extracted from γ-Irradiated Treponema pallidum and Treponema reiteri

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• Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific Activity

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• The relationship of treponemal immobilizing antibody to immunity in syphilis.

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• Protective antibodies in the serum of syphilitic patients.

  1948cited by this paper
• Immunity in Syphilis

  1927cited by this paper

• Molecular and Immunological Strategies Against Treponema pallidum Infections

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• Sexually Transmitted Infections: Advances in Understanding and Management

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• The Treponema pallidum Outer Membrane.

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• Advances and applications of molecular cloning in clinical microbiology

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• Chapter 59 – Syphilis

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• Crystal Structure of the 47-kDa Lipoprotein of Treponema pallidum Reveals a Novel Penicillin-binding Protein*

  2002cites this paper
• Expression in Escherichia coli of flaB, the gene coding for a periplasmic flagellin of Leptospira interrogans serovar pomona.

  1999cites this paper
• A mgl-like operon in Treponema pallidum, the syphilis spirochete.

  1996cites this paper
• Purification of Treponema pallidum, Nichols strain, by two-step column chromatography.

  1995cites this paper
• Similarity between the 38-kilodalton lipoprotein of Treponema pallidum and the glucose/galactose-binding (MglB) protein of Escherichia coli

  1994influential citation
• The 47-kDa major lipoprotein immunogen of Treponema pallidum is a penicillin-binding protein with carboxypeptidase activity.

  1994cites this paper
• Polypeptides of Treponema pallidum: progress toward understanding their structural, functional, and immunologic roles. Treponema Pallidum Polypeptide Research Group.

  1993cites this paper
• Lipid modification of the 17-kilodalton membrane immunogen of Treponema pallidum determines macrophage activation as well as amphiphilicity

  1993cites this paper
• Polypeptides of Treponema pallidum: progress toward understanding their structural, functional, and immunologic roles. Treponema Pallidum Polypeptide Research Group

  1993cites this paper
• Molecular biology of Treponema pallidum.

  1992cites this paper
• Molecular cloning and characterization of DNA from human intestinal spirochetes

  1992cites this paper
• Analysis of the N-terminal region of the 47-kilodalton integral membrane lipoprotein of Treponema pallidum

  1992influential citation
• Ocular syphilis.

  1992cites this paper
• Molecular cloning, partial purification, and characterization of a haemin‐binding lipoprotein from Haemophilus influenzae type b

  1991cites this paper
• Phagocytosis of opsonized Treponema pallidum subsp. pallidum proceeds slowly

  1990cites this paper
• Molecular and antigenic analysis of treponemes.

  1990cites this paper
• The 34-kilodalton membrane immunogen of Treponema pallidum is a lipoprotein

  1990cites this paper
• Expression in Escherichia coli of the 37-kilodalton endoflagellar sheath protein of Treponema pallidum by use of the polymerase chain reaction and a T7 expression system

  1990cites this paper
• Cloning and expression of Treponema pallidum antigens in Escherichia coli.

  1989cites this paper
• Treponema pallidum subspecies pallidum (Nichols) and Treponema pallidum subspecies pertenue (CDC 2575) differ in at least one nucleotide: comparison of two homologous antigens.

  1989cites this paper
• Outer membrane ultrastructure explains the limited antigenicity of virulent Treponema pallidum.

  1989cites this paper
• Sequence analysis of the 47-kilodalton major integral membrane immunogen of Treponema pallidum

  1989cites this paper
• Sensitivity and specificity of an enzyme-linked immunosorbent assay using the recombinant DNA-derived Treponema pallidum protein TmpA for serodiagnosis of syphilis and the potential use of TmpA for assessing the effect of antibiotic therapy

  1989cites this paper
• Acylation of the 47-kilodalton major membrane immunogen of Treponema pallidum determines its hydrophobicity

  1989cites this paper
• Molecular cloning and DNA sequence analysis of the 37-kilodalton endoflagellar sheath protein gene of Treponema pallidum

  1989cites this paper
• Overproduction and purification of Treponema pallidum recombinant-DNA-derived proteins TmpA and TmpB and their potential use in serodiagnosis of syphilis

  1989cites this paper
• Characterization of lipid-modified immunogenic proteins of Treponema pallidum expressed in Escherichia coli.

  1989cites this paper
• The development of biosynthetic vaccines.

  1989cites this paper
• Immunogenicity of three recombinant Treponema pallidum antigens examined in guinea pigs.

  1989cites this paper
• Molecular cloning and characterization of the 15-kilodalton major immunogen of Treponema pallidum

  1989influential citation
• Molecular approaches to improved syphilis serodiagnosis

  1989cites this paper
• Immunity to Syphilitic Infection

  1988cites this paper
• Genetic and physicochemical characterization of the recombinant DNA-derived 47-kilodalton surface immunogen of Treponema pallidum subsp. pallidum

  1988cites this paper
• Immunization of rabbits with recombinant Treponema pallidum surface antigen 4D alters the course of experimental syphilis.

  1988cites this paper
• IMMUNIZATION OF RABBITS SURFACE ANTIGEN 4D ALTERS WITH RECOMBINANT Treponema patlidurn THE COURSE OF EXPERIMENTAL

  1988cites this paper
• Identification and localization of integral membrane proteins of virulent Treponema pallidum subsp. pallidum by phase partitioning with the nonionic detergent triton X-114

  1988cites this paper
• Isolation and characterization of recombinant Escherichia coli clones secreting a 24-kilodalton antigen of Treponema pallidum

  1988cites this paper
• Identity of Treponema pallidum subsp. pallidum polypeptides: Correlation of sodium dodecyl sulfate‐polyacrylamide gel electrophoresis results from different laboratories

  1987cites this paper
• Identification and purification of a recombinant Treponema pallidum basic membrane protein antigen expressed in Escherichia coli

  1987cites this paper
• Cloning and expression of treponema pallidum common antigen (Tp-4) in Escherichia coli K12.

  1987cites this paper
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  1987cites this paper
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  1987cites this paper
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  1987cites this paper
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  1987cites this paper
• The eighth C. L. Oakley lecture. Pathogenicity and immunobiology of Treponema pallidum.

  1987cites this paper
• Antigenic interrelationship between endoflagella of Treponema phagedenis biotype Reiter and Treponema pallidum (Nichols): molecular characterization of endoflagellar proteins

  1986cites this paper
• Serological characterization and gene localization of an Escherichia coli-expressed 37-kilodalton Treponema pallidum antigen

  1986cites this paper
• The surface of virulent Treponema pallidum: resistance to antibody binding in the absence of complement and surface association of recombinant antigen 4D

  1986cites this paper
• Development of increased serum immunoblot reactivity against a 45,000-dalton polypeptide of Treponema pallidum (Nichols) correlates with establishment of chancre immunity in syphilitic rabbits

  1986cites this paper
• Cloning and expression of the major 47-kilodalton surface immunogen of Treponema pallidum in Escherichia coli

  1986cites this paper
• Immunochemical characterization and purification of Treponema pallidum antigen TpD expressed by Escherichia coli K12.

  1986cites this paper
• Monoclonal antibody selection and analysis of a recombinant DNA-derived surface immunogen of Treponema pallidum expressed in Escherichia coli

  1986cites this paper
• Isolation of a Treponema pallidum gene encoding immunodominant outer envelope protein P6, which reacts with sera from patients at different stages of syphilis

  1986cites this paper
• Molecular and immunochemical analysis of Treponema pallidum

  1986cites this paper
• Genetic characterization and partial sequence determination of a Treponema pallidum operon expressing two immunogenic membrane proteins in Escherichia coli

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• Cloning and surface expression in Escherichia coli of a structural gene encoding a surface protein of Haemophilus influenzae type b

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  1985cites this paper
• Cellular and extracellular protein antigens of Treponema pallidum synthesized during in vitro incubation of freshly extracted organisms

  1985cites this paper
• Purification and characterization of a cloned protease-resistant Treponema pallidum-specific antigen

  1984cites this paper
• Sensitivity and specificity of monoclonal antibodies directed against antigenic determinants of Treponema pallidum Nichols in the diagnosis of syphilis

  1984cites this paper
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  1984cites this paper
• Antigenic complexity of Treponema pallidum: antigenicity and surface localization of major polypeptides.

  1984cites this paper
• The impact of new cloning techniques on the diagnosis and treatment of infectious diseases.

  1984cites this paper
• Cloning and Expression of Treponernu pullidum Common Antigen ( Tp-4 ) in Escherichia coli K 12

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• Pathogenicity and immunobiology of Treponema

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