Cloning and expression of the major 47-kilodalton surface immunogen of Treponema pallidum in Escherichia coli

No abstract is available for this paper.

• Publication year

  1986

• Venue

  Infection and Immunity

• Publication date

  1986-11-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1128/iai.54.2.500-506.1986PMID 3021631PMCID PMC260189
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Molecular specificities of monoclonal antibodies directed against virulent Treponema pallidum

  1986cited by this paper
• Characterization of the Humoral Immune Response of the Rabbit to Antigens of Treponema pallidum after Experimental Infection and Therapy

  1986influential reference
• Development of increased serum immunoblot reactivity against a 45,000-dalton polypeptide of Treponema pallidum (Nichols) correlates with establishment of chancre immunity in syphilitic rabbits

  1986cited by this paper
• Monoclonal antibody selection and analysis of a recombinant DNA-derived surface immunogen of Treponema pallidum expressed in Escherichia coli

  1986cited by this paper
• Monoclonal antibodies to Treponema pallidum: recognition of a major polypeptide antigen.

  1985cited by this paper
• Characterization of monoclonal antibodies to Treponema pallidum.

  1985influential reference
• Detection of Treponema pallidum in lesion exudate with a pathogen-specific monoclonal antibody

  1985cited by this paper
• Genetic characterization and partial sequence determination of a Treponema pallidum operon expressing two immunogenic membrane proteins in Escherichia coli

  1985cited by this paper
• Antigens of Treponema pallidum recognized by IgG and IgM antibodies during syphilis in humans.

  1985cited by this paper
• The outer membrane of Treponema pallidum: biological significance and biochemical properties.

  1985cited by this paper
• A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.

  1985cited by this paper
• Monoclonal antibody with hemagglutination, immobilization, and neutralization activities defines an immunodominant, 47,000 mol wt, surface-exposed immunogen of Treponema pallidum (Nichols)

  1984cited by this paper
• Sensitivity and specificity of monoclonal antibodies directed against antigenic determinants of Treponema pallidum Nichols in the diagnosis of syphilis

  1984cited by this paper
• Purification and characterization of a cloned protease-resistant Treponema pallidum-specific antigen

  1984cited by this paper
• Molecular analysis of immunoglobulins M and G immune response to protein antigens of Treponema pallidum in human syphilis

  1984cited by this paper
• Antigenic cross-reactivity between Treponema pallidum and other pathogenic members of the family Spirochaetaceae

  1984cited by this paper
• Antigenic complexity of Treponema pallidum: antigenicity and surface localization of major polypeptides.

  1984cited by this paper
• Purification of Treponema pallidum, Nichols Strain, by Percoll Density Gradient Centrifugation

  1984cited by this paper
• Monoclonal antibody analysis of specific antigenic similarities among pathogenic Treponema pallidum subspecies

  1984cited by this paper
• Quantitative isolation of DNA restriction fragments from low-melting agarose by Elutip-d affinity chromatography.

  1983cited by this paper
• The biology, pathology, and immunology of syphilis.

  1983cited by this paper
• Cloning and expression of Treponema pallidum (Nichols) antigen genes in Escherichia coli

  1983cited by this paper
• Identification and preliminary characterization of Treponema pallidum protein antigens expressed in Escherichia coli

  1983cited by this paper
• Comparison of major protein antigens and protein profiles of Treponema pallidum and Treponema pertenue

  1983cited by this paper
• Molecular basis of immunological cross-reactivity between Treponema pallidum and Treponema pertenue

  1983cited by this paper
• Identification of Treponema pallidum antigens: comparison with a nonpathogenic treponeme.

  1982cited by this paper
• Murine monoclonal antibodies specific for virulent Treponema pallidum (Nichols)

  1982cited by this paper
• Humoral immune response in human syphilis to polypeptides of Treponema pallidum.

  1982cited by this paper
• Rapid and simple removal of contaminating RNA from plasmid DNA without the use of RNase.

  1981cited by this paper
• Plasmid DNA in Treponema pallidum (Nichols): potential for antibiotic resistance by syphilis bacteria.

  1981cited by this paper
• Surface characterization of virulent Treponema pallidum.

  1980cited by this paper
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• The conjugation system of F-like plasmids.

  1980cited by this paper
• Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose.

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• Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film

  1977cited by this paper
• Humoral immunity in experimental syphilis. II. The relationship of neutralizing factors in immune serum to acquired resistance.

  1976cited by this paper
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• Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific Activity

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• Extracellular Loops of the Treponema pallidum FadL Orthologs TP0856 and TP0858 Elicit IgG Antibodies and IgG+-Specific B-Cells in the Rabbit Model of Experimental Syphilis

  2022cites this paper
• Sexually Transmitted Infections: Advances in Understanding and Management

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• Molecular and Immunological Strategies Against Treponema pallidum Infections

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• The Jarisch-Herxheimer reaction: revisited.

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• The JarischeHerxheimer reaction: Revisited

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• Evaluation of a colloidal gold immunochromatography assay in the detection of Treponema pallidum specific IgM antibody in syphilis serofast reaction patients: a serologic marker for the relapse and infection of syphilis.

  2011cites this paper
• Development of a colloidal gold-immunochromatography assay to detect immunoglobulin G antibodies to Treponema pallidum with TPN17 and TPN47.

  2010cites this paper
• Chapter 59 – Syphilis

  2009cites this paper
• Profile of anti-Tp47 antibodies in patients with positive serology for syphilis analized by Western Blot.

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• Recombinant antigen-based immuno-slot blot method for serodiagnosis of syphilis.

  2004cites this paper
• Etiology of Genital Ulcer Disease and Association With Human Immunodeficiency Virus Infection in Two Tanzanian Cities

  2003cites this paper
• Crystal Structure of the 47-kDa Lipoprotein of Treponema pallidum Reveals a Novel Penicillin-binding Protein*

  2002cites this paper
• Immunization with Treponema pallidum outer membrane vesicles induces high-titer complement-dependent treponemicidal activity and aggregation of T. pallidum rare outer membrane proteins (TROMPs).

  1999cites this paper
• Expression in Escherichia coli of flaB, the gene coding for a periplasmic flagellin of Leptospira interrogans serovar pomona.

  1999cites this paper
• pallidum Activity and Aggregation of Complement-Dependent Treponemicidal High-TiterOuter Membrane Vesicles Induces Treponema pallidum Immunization with

  1999cites this paper
• Reactivity of recombinant treponema pallidum (r‐Tp) antigens with anti‐Tp antibodies in human syphilitic sera evaluated by ELISA

  1997cites this paper
• Immunological studies on venereal spirochetosis of rabbits (rabbit syphilis).

  1996cites this paper
• Molecular mimicry between an immunodominant amino acid motif on the 47-kDa lipoprotein of Treponema pallidum (Tpp47) and multiple repeats of analogous sequences in fibronectin.

  1996cites this paper
• Purification of Treponema pallidum, Nichols strain, by two-step column chromatography.

  1995cites this paper
• Recombinant antigen-based enzyme immunoassay for screening of Treponema pallidum antibodies in blood bank routine

  1995cites this paper
• Physical map of the genome of Treponema pallidum subsp. pallidum (Nichols)

  1995cites this paper
• Polypeptides of Treponema pallidum: progress toward understanding their structural, functional, and immunologic roles. Treponema Pallidum Polypeptide Research Group.

  1993cites this paper
• Polypeptides of Treponema pallidum: progress toward understanding their structural, functional, and immunologic roles. Treponema Pallidum Polypeptide Research Group

  1993cites this paper
• The Genus Treponema

  1992cites this paper
• Analysis of the N-terminal region of the 47-kilodalton integral membrane lipoprotein of Treponema pallidum

  1992cites this paper
• Molecular biology of Treponema pallidum.

  1992cites this paper
• Molecular and Cell Biology of Sexually Transmitted Diseases

  1992cites this paper
• Sensitive detection of Treponema pallidum by using the polymerase chain reaction

  1991cites this paper
• Chemical and biological activities of a 64-kilodalton outer sheath protein from Treponema denticola strains

  1991cites this paper
• Molecular and antigenic analysis of treponemes.

  1990cites this paper
• The 34-kilodalton membrane immunogen of Treponema pallidum is a lipoprotein

  1990cites this paper
• Immunogenic integral membrane proteins of Borrelia burgdorferi are lipoproteins

  1990cites this paper
• Evaluation of immunoglobulin M western blot analysis in the diagnosis of congenital syphilis

  1990cites this paper
• Primary structure of an oligomeric antigen of Treponema pallidum

  1989cites this paper
• Molecular characterization of the pathogen-specific, 34-kilodalton membrane immunogen of Treponema pallidum

  1989influential citation
• Cloning and expression of Treponema pallidum antigens in Escherichia coli.

  1989cites this paper
• Acylation of the 47-kilodalton major membrane immunogen of Treponema pallidum determines its hydrophobicity

  1989cites this paper
• Sequence analysis of the 47-kilodalton major integral membrane immunogen of Treponema pallidum

  1989influential citation
• Molecular analysis of the fetal IgM response to Treponema pallidum antigens: implications for improved serodiagnosis of congenital syphilis.

  1989cites this paper
• Molecular approaches to improved syphilis serodiagnosis

  1989cites this paper
• Major integral membrane protein immunogens of Treponema pallidum are proteolipids

  1989cites this paper
• The development of biosynthetic vaccines.

  1989cites this paper
• Genetic and physicochemical characterization of the recombinant DNA-derived 47-kilodalton surface immunogen of Treponema pallidum subsp. pallidum

  1988cites this paper
• Identification and localization of integral membrane proteins of virulent Treponema pallidum subsp. pallidum by phase partitioning with the nonionic detergent triton X-114

  1988cites this paper
• Selective release of the Treponema pallidum outer membrane and associated polypeptides with Triton X-114

  1988cites this paper
• Host-Parasite Relationships in Sexually Transmitted Diseases

  1988cites this paper
• Identification, cloning, and purification of protein antigens of Treponema pallidum.

  1988influential citation
• Expression of Antigen Genes of Leptospira interrogans Serovar canicola in Escherichia coli

  1988cites this paper
• Isolation and characterization of recombinant Escherichia coli clones secreting a 24-kilodalton antigen of Treponema pallidum

  1988cites this paper
• Identity of Treponema pallidum subsp. pallidum polypeptides: Correlation of sodium dodecyl sulfate‐polyacrylamide gel electrophoresis results from different laboratories

  1987cites this paper