A study of iterative type II polyketide synthases, using bacterial genes cloned from soil DNA: a means to access and use genes from uncultured microorganisms

No abstract is available for this paper.

• Publication year

  1997

• Venue

  Journal of Bacteriology

• Publication date

  1997-12-01

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1128/jb.179.23.7360-7368.1997PMID 9393700PMCID PMC179686
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Genetic complementation ofStreptomyces tendae deficient in nikkomycin production

  2004cited by this paper
• ANTIBIOTICS FROM GENETICALLY ENGINEERED MICROORGANISMS

  1997cited by this paper
• Minimal Streptomyces sp. strain C5 daunorubicin polyketide biosynthesis genes required for aklanonic acid biosynthesis

  1997cited by this paper
• Domain Analysis of the Molecular Recognition Features of Aromatic Polyketide Synthase Subunits*

  1997cited by this paper
• RATIONAL DESIGN AND ENGINEERED BIOSYNTHESIS OF A NOVEL 18-CARBON AROMATIC POLYKETIDE

  1997cited by this paper
• A gene cluster involved in nogalamycin biosynthesis fromStreptomyces nogalater: sequence analysis and complementation of early-block mutations in the anthracycline pathway

  1996cited by this paper
• Engineered biosynthesis of peptide antibiotics.

  1996cited by this paper
• Twenty-five coregulated transcripts define a sterigmatocystin gene cluster in Aspergillus nidulans.

  1996cited by this paper
• Deciphering the mechanism for the assembly of aromatic polyketides by a bacterial polyketide synthase.

  1996cited by this paper
• Characterization of Streptomyces argillaceus genes encoding a polyketide synthase involved in the biosynthesis of the antitumor mithramycin.

  1996cited by this paper
• Rational design of peptide antibiotics by targeted replacement of bacterial and fungal domains.

  1995cited by this paper
• Polyketide synthase gene manipulation: a structure-function approach in engineering novel antibiotics.

  1995cited by this paper
• Functional analysis of putative beta-ketoacyl:acyl carrier protein synthase and acyltransferase active site motifs in a type II polyketide synthase of Streptomyces glaucescens

  1995cited by this paper
• A Sorangium cellulosum (myxobacterium) gene cluster for the biosynthesis of the macrolide antibiotic soraphen A: cloning, characterization, and homology to polyketide synthase genes from actinomycetes

  1995cited by this paper
• Repositioning of a domain in a modular polyketide synthase to promote specific chain cleavage.

  1995cited by this paper
• Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.

  1995cited by this paper
• The Streptomyces glaucescens tcmKL polyketide synthase and tcmN polyketide cyclase genes govern the size and shape of aromatic polyketides

  1995cited by this paper
• ENGINEERED BIOSYNTHESIS OF NOVEL POLYKETIDES : ANALYSIS OF TCMN FUNCTION IN TETRACENOMYCIN BIOSYNTHESIS

  1995cited by this paper
• The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin.

  1995cited by this paper
• Heterologous expression of an engineered biosynthetic pathway: functional dissection of type II polyketide synthase components in Streptomyces species

  1995cited by this paper
• Rational design of aromatic polyketide natural products by recombinant assembly of enzymatic subunits

  1995cited by this paper
• Manipulation of macrolide ring size by directed mutagenesis of a modular polyketide synthase

  1995cited by this paper
• Cloning and characterization of a polyketide synthase gene from Streptomyces fradiae Tü2717, which carries the genes for biosynthesis of the angucycline antibiotic urdamycin A and a gene probably involved in its oxygenation

  1995cited by this paper
• Cloning, sequencing, and analysis of the griseusin polyketide synthase gene cluster from Streptomyces griseus

  1994cited by this paper
• Correlation of the Avermectin Polyketide Synthase Genes to the Avermectin Structure: Implications for Designing Novel Avermectins

  1994cited by this paper
• Characterization of the Streptomyces peucetius ATCC 29050 genes encoding doxorubicin polyketide synthase.

  1994cited by this paper
• Isolation and sequence analysis of polyketide synthase genes from the daunomycin-producing Streptomyces sp. strain C5

  1994cited by this paper
• Engineered biosynthesis of novel polyketides: evidence for temporal, but not regiospecific, control of cyclization of an aromatic polyketide precursor.

  1994cited by this paper
• Cloning and nucleotide sequence of a region of the Kibdelosporangium aridum genome homologous to polyketide biosynthetic genes.

  1994cited by this paper
• Sequences of the oxytetracycline polyketide synthase-encoding otc genes from Streptomyces rimosus.

  1994cited by this paper
• ENGINEERED BIOSYNTHESIS OF A TRIKETIDE LACTONE FROM AN INCOMPLETE MODULAR POLYKETIDE SYNTHASE

  1994cited by this paper
• Strategies for the discovery of secondary metabolites from marine bacteria: ecological perspectives.

  1994cited by this paper
• Engineered biosynthesis of novel polyketides: influence of a downstream enzyme on the catalytic specificity of a minimal aromatic polyketide synthase.

  1994cited by this paper
• Engineered Biosynthesis of Novel Polyketides: actVII and actIV Genes Encode Aromatase and Cyclase Enzymes, Respectively

  1994cited by this paper
• Cloning, sequencing and deduced functions of a cluster of Streptomyces genes probably encoding biosynthesis of the polyketide antibiotic frenolicin.

  1994cited by this paper
• Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230.

  1994cited by this paper
• Polyketide synthesis: prospects for hybrid antibiotics.

  1993cited by this paper
• Saccharopolyspora hirsuta 367 encodes clustered genes similar to ketoacyl synthase, ketoacyl reductase, acyl carrier protein, and biotin carboxyl carrier protein

  1993cited by this paper
• Engineered biosynthesis of novel polyketides.

  1993cited by this paper
• A hydroxylase-like gene product contributes to synthesis of a polyketide spore pigment in Streptomyces halstedii

  1993cited by this paper
• The tcmVI region of the tetracenomycin C biosynthetic gene cluster of Streptomyces glaucescens encodes the tetracenomycin F1 monooxygenase, tetracenomycin F2 cyclase, and, most likely, a second cyclase

  1993cited by this paper
• Nucleotide sequences and heterologous expression of tcmG and tcmP, biosynthetic genes for tetracenomycin C in Streptomyces glaucescens

  1993cited by this paper
• Functional replacement of genes for individual polyketide synthase components in Streptomyces coelicolor A3(2) by heterologous genes from a different polyketide pathway

  1992cited by this paper
• Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.

  1992cited by this paper
• Characterisation of actI-homologous DNA encoding polyketide synthase genes from the monensin producer Streptomyces cinnamonensis

  1992cited by this paper
• Nucleotide sequence and deduced functions of a set of cotranscribed genes of Streptomyces coelicolor A3(2) including the polyketide synthase for the antibiotic actinorhodin.

  1992cited by this paper
• Analysis of a polyketide synthesis-encoding gene cluster of Streptomyces curacoi.

  1992cited by this paper
• Nucleotide sequence of the tcmII-tcmIV region of the tetracenomycin C biosynthetic gene cluster of Streptomyces glaucescens and evidence that the tcmN gene encodes a multifunctional cyclase-dehydratase-O-methyl transferase

  1992cited by this paper
• The polyketide metabolites

  1991cited by this paper
• Modular organization of genes required for complex polyketide biosynthesis.

  1991cited by this paper
• An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea

  1990cited by this paper
• The multifunctional 6-methylsalicylic acid synthase gene of Penicillium patulum. Its gene structure relative to that of other polyketide synthases.

  1990cited by this paper
• Bioactive microbial products: focus upon mechanism of action.

  1990cited by this paper
• Spore colour in Streptomyces coelicolor A3(2) involves the developmentally regulated synthesis of a compound biosynthetically related to polyketide antibiotics

  1990cited by this paper
• High diversity in DNA of soil bacteria

  1990cited by this paper
• Analysis of the nucleotide sequence of the Streptomyces glaucescens tcmI genes provides key information about the enzymology of polyketide antibiotic biosynthesis.

  1989cited by this paper
• A Laboratory manual

  1989cited by this paper
• Structure and deduced function of the granaticin‐producing polyketide synthase gene cluster of Streptomyces violaceoruber Tü22.

  1989cited by this paper
• Cloning of genes governing the deoxysugar portion of the erythromycin biosynthesis pathway in Saccharopolyspora erythraea (Streptomyces erythreus)

  1989cited by this paper
• β-ketoacyl-ACP synthase I of Escherichia coli: Nucleotide sequence of thefabB gene and identification of the cerulenin binding residue

  1988cited by this paper
• DNA Probe Method for the Detection of Specific Microorganisms in the Soil Bacterial Community

  1988cited by this paper
• The neighbor-joining method: a new method for reconstructing phylogenetic trees.

  1987cited by this paper
• Cloning and heterologous expression of a gene cluster for the biosynthesis of tetracenomycin C, the anthracycline antitumor antibiotic of Streptomyces glaucescens.

  1987cited by this paper
• Isolation of tetracenomycin C-nonproducing Streptomyces glaucescens mutants

  1986cited by this paper
• Biosynthesis of fredericamycin A, a new antitumor antibiotic.

  1985cited by this paper
• Genetic manipulation of Streptomyces : a laboratory manual

  1985cited by this paper
• CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP

  1985cited by this paper
• Biotechnology of industrial antibiotics

  1984cited by this paper
• A comprehensive set of sequence analysis programs for the VAX

  1984cited by this paper
• Plasmids, recombination and chromosome mapping in Streptomyces lividans 66.

  1983cited by this paper
• Experimental Microbial Ecology

  1970cited by this paper
• Curamycin—I: Isolation and characterization of some hydrolysis products

  1961cited by this paper

• Insights into the phylogenetic diversity, biological activities, and biosynthetic potential of mangrove rhizosphere Actinobacteria from Hainan Island

  2023cites this paper
• Cultivable bacteria associated with Caribbean octocorals are active against coral pathogens but exhibit variable bioactivity when grown under different temperature conditions

  2022cites this paper
• Optimization of Degenerate PCR Conditions for Reducing Error Rates in Detection of PKS and NRPS Gene groups in Actinomycetes

  2022cites this paper
• Great diversity of KSα sequences from bat-associated microbiota suggests novel sources of uncharacterized natural products

  2022cites this paper
• Insights into the Variation in Bioactivities of Closely Related Streptomyces Strains from Marine Sediments of the Visayan Sea against ESKAPE and Ovarian Cancer

  2021cites this paper
• The natural product biosynthesis potential of the microbiomes of Earth – Bioprospecting for novel anti-microbial agents in the meta-omics era

  2021cites this paper
• Biotechnology.

  2020cites this paper
• Overview of the Antimicrobial Compounds Produced by Members of the Bacillus subtilis Group

  2019cites this paper
• Metagenomics

  2019cites this paper
• Bioactivity and genome guided isolation of a novel antimicrobial protein from Thalassomonas viridans

  2019influential citation
• Single-Cell Metagenomics

  2019cites this paper
• Microbes as a novel source of secondary metabolite products of industrial significance

  2019cites this paper
• Metagenomics for Novel Enzymes: A Current Perspective

  2019cites this paper
• Isolation and biochemical characterization of a metagenome-derived 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase gene from subtropical marine mangrove wetland sediments

  2019cites this paper
• Metagenomics a modern approach to reveal the secrets of unculturable microbes

  2019cites this paper
• Discovery of biomolecules from South Africanmarine sponge symbionts throughMetagenomic studies

  2018cites this paper
• Chapter 14 – Novel Biological Resources Screened From Uncultured Bacteria by a Metagenomic Method

  2018cites this paper
• Bacterial Diversity and Phylogenetic Analysis of Type II Polyketide Synthase Gene from Manao-Pee Cave, Thailand

  2018influential citation
• New approaches to antimicrobial discovery

  2017cites this paper
• AN INTEGRATED BIOINFORMATIC/EXPERIMENTAL APPROACH FOR DISCOVERING NOVEL TYPE II POLYKETIDES ENCODED IN ACTINOBACTERIAL GENOMES

  2017influential citation
• Genomic and functional techniques to mine the microbiome for novel antimicrobials and antimicrobial resistance genes

  2017cites this paper
• Using natural products for drug discovery: the impact of the genomics era

  2017cites this paper
• Current Advances in Biotechnology-Driven Marine Microbial Metagenomics

  2016cites this paper
• Bacteria associated with lionfish (Pterois volitans/miles complex) exhibit antibacterial activity against known fish pathogens

  2016cites this paper
• Culture-independent discovery of natural products from soil metagenomes

  2016cites this paper
• Novel Cellulases through culture-dependent and metagenomics based approaches: Discovery and Characterization

  2016cites this paper
• Environmental Microbiology: Fundamentals and Applications

  2015cites this paper
• Some Historical Elements of Microbial Ecology

  2015cites this paper
• Acylation of Biomolecules in Prokaryotes: a Widespread Strategy for the Control of Biological Function and Metabolic Stress

  2015cites this paper
• Mining the metabiome: identifying novel natural products from microbial communities.

  2014cites this paper
• Arixanthomycins A–C: Phylogeny-Guided Discovery of Biologically Active eDNA-Derived Pentangular Polyphenols

  2014cites this paper
• Mining Soil Metagenomes to Better Understand the Evolution of Natural Product Structural Diversity: Pentangular Polyphenols as a Case Study

  2014cites this paper
• Développement et premières applications d'une méthode de tri de cellules bactériennes par marquage de l'ADN avec des nanoparticules magnétiques pour l'étude de la diversité bactérienne environnementale et des transferts horizontaux de gènes in situ

  2013cites this paper
• Metagenomics: Techniques, Applications and Challenges

  2013cites this paper
• Cultural and metagenomic approaches for isolation of genes encoding enzymes involved in hydrolysis of cellulose

  2013cites this paper
• Arimetamycin A: improving clinically relevant families of natural products through sequence-guided screening of soil metagenomes.

  2013cites this paper
• Antibacterial Discovery: Problems and Possibilities

  2013cites this paper
• Functional Analysis of Environmental DNA-Derived Microviridins Provides New Insights into the Diversity of the Tricyclic Peptide Family

  2013cites this paper
• Acetoacetyl‐CoA synthetase activity is controlled by a protein acetyltransferase with unique domain organization in Streptomyces lividans

  2013cites this paper
• Novel Molecules from Environmental DNA-Derived Type II Polyketide Biosynthetic Gene Clusters

  2013cites this paper
• Platforms for antibiotic discovery

  2013cites this paper
• Insight into the molecular basis of aromatic polyketide cyclization: crystal structure and in vitro characterization of WhiE-ORFVI.

  2012cites this paper
• Metagenomics in Streptomyces lividans reveals host-dependent functional expression 1 2 3 Running Title : Functional metagenomics in Streptomyces lividans 4 5

  2012cites this paper
• Discovery of Enzymes

  2012cites this paper
• Metagenomic Analysis of Streptomyces lividans Reveals Host-Dependent Functional Expression

  2012cites this paper
• Efforts Toward Production of Novel Natural Products From Uncultured Soil Microbes

  2012cites this paper
• Functional Intestinal Metagenomics

  2011cites this paper
• Methods and options for the heterologous production of complex natural products.

  2011cites this paper
• From Prospecting to Product – Industrial Metagenomics Is Coming of Age

  2011cites this paper
• Approaches to capturing and designing biologically active small molecules produced by uncultured microbes.

  2011cites this paper
• Polyketide synthase pathways identi¢ed from a metagenomic library are derived from soil Acidobacteria

  2011cites this paper
• Strategies for discovery and improvement of enzyme function: state of the art and opportunities

  2011cites this paper
• Functional characterization of a new holin-like antibacterial protein coding gene tmp1 from goat skin surface metagenome

  2011cites this paper
• Chapter three - Quo vadis Soil Organic Matter Research?: A Biological Link to the Chemistry of Humification

  2011cites this paper
• Polyketide synthase pathways identified from a metagenomic library are derived from soil Acidobacteria.

  2011cites this paper
• Natural Product Biosynthesis In Uncultured Bacteria

  2011cites this paper
• Drug Discovery and Development by Combinatorial Biosynthesis

  2011cites this paper
• Composition of dissolved organic matter in groundwater

  2011cites this paper
• Quo vadis Soil Organic Matter Research

  2011cites this paper
• SELEX-Based Identification of Synthetic Binding Sites for Streptomyces acidiscabies 84.104 γ-Butyrolactone Receptors: SabS and SabR.

  2011cites this paper
• Special issue of The Journal of Antibiotics dedicated to the late Professor C Richard Hutchinson.

  2011cites this paper
• Functional analysis of environmental DNA-derived type II polyketide synthases reveals structurally diverse secondary metabolites

  2011cites this paper
• Special issue of The Journal of Antibiotics dedicated to the late Professor C Richard Hutchinson

  2011cites this paper
• Tailoring enzyme-rich environmental DNA clones: a source of enzymes for generating libraries of unnatural natural products.

  2010cites this paper
• Discovery and Characterization of Novel Antibiotic Synthesis and Resistance Determinants from Diverse Microbial Metagenomes

  2010cites this paper
• Expanding Small-Molecule Functional Metagenomics through Parallel Screening of Broad-Host-Range Cosmid Environmental DNA Libraries in Diverse Proteobacteria

  2010cites this paper
• Cloning large natural product gene clusters from the environment: Piecing environmental DNA gene clusters back together with TAR

  2010cites this paper
• Unlocking Environmental DNA Derived Gene Clusters Using a Metagenomics Approach

  2010cites this paper
• Pure culture and metagenomic approaches to investigate cellulose and xylan degradation

  2010cites this paper
• Fluostatins produced by the heterologous expression of a TAR reassembled environmental DNA derived type II PKS gene cluster.

  2010cites this paper
• SCREENING OF SECONDARY METABOLITE-PRODUCING BACTERIA ASSOCIATED WITH CORALS USING 16S rDNA-BASED APPROACH

  2010cites this paper
• An environmental DNA-derived type II polyketide biosynthetic pathway encodes the biosynthesis of the pentacyclic polyketide erdacin.

  2009cites this paper
• Characterization of biosynthetic and catabolic pathways of Bacillus subtilis strain 168.

  2009influential citation
• Bioprospecção de genes biossintéticos de policetídeos em DNA metagenômico de solo de Mata Atlântica.

  2009cites this paper
• Detection of expressed fungal Type I polyketide synthase genes in a forest soil

  2009cites this paper
• Metagenomics: Concept, methodology, ecological inference and recent advances

  2009cites this paper
• Detection and Expression of Biosynthetic Genes in Actinobacteria

  2009influential citation
• Polyketide Synthase Pathway Discovery from Soil Metagenomic Libraries

  2009cites this paper
• Metagenomic approaches to natural products from free-living and symbiotic organisms.

  2009cites this paper
• Novel genes retrieved from environmental DNA by polymerase chain reaction: current genome-walking techniques for future metagenome applications.

  2009cites this paper
• Detection of Antibiotics Produced by Soil and Rhizosphere Microbes In Situ

  2008cites this paper
• Characterization of a Gene Conferring Red Fluorescence Isolated from an Environmental DNA Library Constructed from Soil Bacteria

  2008cites this paper
• Identification of genes coding for hydrolytic dehalogenation in the metagenome derived from a denitrifying 4-chlorobenzoate degrading consortium.

  2008cites this paper
• A procedure for the metagenomics exploration of disease-suppressive soils.

  2008cites this paper
• Marine metagenomics: strategies for the discovery of novel enzymes with biotechnological applications from marine environments

  2008cites this paper
• Microbial biocontrol of the pathogen Phytophthora citricola in the rhizosphere of European beech (Fagus sylvatica L.)

  2008cites this paper
• Isolation and Characterization of a Novel Thermostable Neopullulanase-Like Enzyme from a Hot Spring in Thailand

  2008cites this paper
• Genetic potential of lichen-forming fungi in polyketide biosynthesis

  2008cites this paper
• Metagenomics and Antibiotic Discovery from Uncultivated Bacteria

  2008cites this paper
• Metagenomics: Future of microbial gene mining

  2008cites this paper
• Forest Soil Metagenome Gene Cluster Involved in Antifungal Activity Expression in Escherichia coli

  2007cites this paper
• Construction of soil environmental DNA cosmid libraries and screening for clones that produce biologically active small molecules

  2007cites this paper
• Approches Métagénomiques en Microbiologie du Sol: Optimisation des Techniques Conventionnelles et Développement d'une Approche Alternative par Capture de Gènes dans une Bactérie Réceptrice.

  2007cites this paper
• Natural products from isnA-containing biosynthetic gene clusters recovered from the genomes of cultured and uncultured bacteria.

  2007cites this paper
• Analyses of the structural organization of unidentified open reading frames from metagenome.

  2007cites this paper
• Characterization of a novel cyclomaltodextrinase expressed from environmental DNA isolated from Bor Khleung hot spring in Thailand.

  2006cites this paper
• Characterization of two novel lipase genes isolated directly from environmental sample

  2006cites this paper
• Metagenomics: Access to Unculturable Microbes in the Environment

  2006cites this paper
• Environmental Whole-Genome Amplification To Access Microbial Populations in Contaminated Sediments

  2006cites this paper
• Microbial diversity and gene mining in Antarctic Dry Valley mineral soils

  2006cites this paper