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GAPDH facilitates homologous recombination repair by stabilizing RAD51 in an HDAC1‐dependent manner

Munan Shi,Jiajia Hou,Weichu Liang,Qianwen Li,Shan Shao,Shusheng Ci,Chuanjun Shu,Xingqi Zhao,Shanmeizi Zhao,Miaoling Huang,Cong-ye Wu,Zhigang Hu,Lingfeng He,Zhigang Guo,Feiyan Pan

Published 2023 in EMBO Reports

ABSTRACT

Homologous recombination (HR), a form of error‐free DNA double‐strand break (DSB) repair, is important for the maintenance of genomic integrity. Here, we identify a moonlighting protein, glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH), as a regulator of HR repair, which is mediated through HDAC1‐dependent regulation of RAD51 stability. Mechanistically, in response to DSBs, Src signaling is activated and mediates GAPDH nuclear translocation. Then, GAPDH directly binds with HDAC1, releasing it from its suppressor. Subsequently, activated HDAC1 deacetylates RAD51 and prevents it from undergoing proteasomal degradation. GAPDH knockdown decreases RAD51 protein levels and inhibits HR, which is re‐established by overexpression of HDAC1 but not SIRT1. Notably, K40 is an important acetylation site of RAD51, which facilitates stability maintenance. Collectively, our findings provide new insights into the importance of GAPDH in HR repair, in addition to its glycolytic activity, and they show that GAPDH stabilizes RAD51 by interacting with HDAC1 and promoting HDAC1 deacetylation of RAD51.

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