Ion-pair reversed-phase high-performance liquid chromatography analysis of oligonucleotides: retention prediction.

No abstract is available for this paper.

• Publication year

  2002

• Venue

  Journal of Chromatography A

• Publication date

  2002-06-01

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1016/S0021-9673(02)00306-0PMID 12134814
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• High-throughput biopolymer desalting by solid-phase extraction prior to mass spectrometric analysis.

  2001cited by this paper
• Genotyping of two mutations in the HFE gene using single-base extension and high-performance liquid chromatography.

  2001cited by this paper
• Analysis and purification of synthetic oligonucleotides by reversed-phase high-performance liquid chromatography with photodiode array and mass spectrometry detection.

  2001cited by this paper
• A kinetic study of mass transfer in reversed-phase liquid chromatography on a C18-silica gel

  2000cited by this paper
• A system for specific, high-throughput genotyping by allele-specific primer extension on microarrays.

  2000cited by this paper
• Kinetic study of the mass transfer of bovine serum albumin in anion-exchange chromatography.

  2000cited by this paper
• Allelic discrimination by denaturing high-performance liquid chromatography.

  2000cited by this paper
• Large-scale purification of antisense oligonucleotides by high-performance membrane adsorber chromatography.

  2000cited by this paper
• Sheath liquid effects in capillary high-performance liquid chromatography-electrospray mass spectrometry of oligonucleotides.

  2000cited by this paper
• DNA sequencing by capillary array electrophoresis and microfabricated array systems

  2000cited by this paper
• Superficially porous silica microspheres for fast high-performance liquid chromatography of macromolecules.

  2000cited by this paper
• Purification of crude DNA oligonucleotides by solid-phase extraction and reversed-phase high-performance liquid chromatography.

  2000cited by this paper
• Homogeneous genotyping assays for single nucleotide polymorphisms with fluorescence resonance energy transfer detection.

  1999cited by this paper
• Isocratic separations on thin glycidyl methacrylate–ethylenedimethacrylate monoliths

  1999cited by this paper
• Reliable noninvasive genotyping based on excremental PCR of nuclear DNA purified with a magnetic bead protocol

  1999cited by this paper
• Design strategies and performance of custom DNA sequencing primers.

  1999cited by this paper
• Analysis of nucleic acids by capillary ion-pair reversed-phase HPLC coupled to negative-ion electrospray ionization mass spectrometry.

  1999cited by this paper
• High-throughput single nucleotide polymorphism genotyping by fluorescent 5' exonuclease assay.

  1999cited by this paper
• Study of phosphorothioate-modified oligonucleotide resistance to 3'-exonuclease using capillary electrophoresis.

  1998cited by this paper
• Intranuclear diffusion and hybridization state of oligonucleotides measured by fluorescence correlation spectroscopy in living cells.

  1998cited by this paper
• Micropellicular stationary phases for high-performance liquid chromatography of double-stranded DNA.

  1998cited by this paper
• Mass spectrometry from miniaturized arrays for full comparative DNA analysis

  1997cited by this paper
• DNA sequencing by capillary electrophoresis

  1997cited by this paper
• New procedure for the use of high-performance liquid chromatography–electrospray ionization mass spectrometry for the analysis of nucleotides and oligonucleotides

  1997cited by this paper
• Model discrimination and estimation of the intraparticle mass transfer parameters for the adsorption of bovine serum albumin onto porous adsorbent particles by the use of experimental frontal analysis data.

  1997cited by this paper
• Ion-pair reversed-phase high-performance liquid chromatography of adenine nucleotides and nucleoside using triethylamine as a counterion.

  1997cited by this paper
• Analysis of Oligonucleotides by HPLC-Electrospray Ionization Mass Spectrometry.

  1997cited by this paper
• Multi-facility survey of oligonucleotide synthesis and an examination of the performance of unpurified primers in automated DNA sequencing.

  1996cited by this paper
• Rapid DNA sequencing of more than 1000 bases per run by capillary electrophoresis using replaceable linear polyacrylamide solutions.

  1996cited by this paper
• The use of elevated column temperature to extend DNA sequencing read lengths in capillary electrophoresis with replaceable polymer matrices

  1996cited by this paper
• High-resolution analysis and purification of synthetic oligonucleotides with strong anion-exchange HPLC.

  1995cited by this paper
• Principles and methods for the analysis and purification of synthetic deoxyribonucleotides by high-performance liquid chromatography

  1995cited by this paper
• Sequencing of oligonucleotides using high performance liquid chromatography and electrospray mass spectrometry.

  1995cited by this paper
• Analysis of oligo(deoxynucleoside phosphorothioate)s and their diastereomeric composition.

  1995cited by this paper
• High-resolution analysis and purification of synthetic oligonucleotides with strong anion-exchange HPLC.

  1995cited by this paper
• Analysis of oligonucleotides using coupled high performance liquid chromatography-electrospray mass spectrometry

  1994cited by this paper
• Effects of linear polyacrylamide concentrations and applied voltages on the separation of oligonucleotides and DNA sequencing fragments by capillary electrophoresis.

  1994cited by this paper
• Prediction of the behaviour of oligonucleotides in high-performance liquid chromatography and capillary electrophoresis.

  1993cited by this paper
• Denaturing HPLC purification of tritylated oligonucleotides using tetraethylammonium hydroxide.

  1993cited by this paper
• Denaturing HPLC purification of tritylated oligonucleotides using tetraethylammonium hydroxide.

  1993cited by this paper
• High-resolution liquid chromatography of DNA fragments on non-porous poly(styrene-divinylbenzene) particles.

  1993cited by this paper
• High-resolution liquid chromatography of oligonucleotides on nonporous alkylated styrene-divinylbenzene copolymers.

  1993cited by this paper
• High-performance liquid chromatographic separation of detritylated oligonucleotides on highly cross-linked poly-(styrene-divinylbenzene) particles.

  1992cited by this paper
• Extension of the electrostatic retention model of reversed-phase ion-pair chromatography to include the simultaneous effect of the organic modifier and the pairing ion

  1990cited by this paper
• Convenient purification of tritylated and detritylated oligonucleotides up to 100-mer.

  1990cited by this paper
• Computer-assisted retention prediction system for oligonucleotides in gradient anion-exchange chromatography.

  1988cited by this paper
• Solid supported hydrolysis of apurinic sites in synthetic oligonucleotides for rapid and efficient purification on reverse-phase cartridges.

  1988cited by this paper
• Reversed-phase ion-pair chromatography of oligodeoxyribonucleotides.

  1987cited by this paper
• A reversed-phase liquid chromatographic columns for the analysis of single-stranded oligo-DNAs

  1987cited by this paper
• A general method for the purification of synthetic oligodeoxyribonucleotides containing strong secondary structure by reversed-phase high-performance liquid chromatography on PRP-1 resin.

  1987cited by this paper
• Use of a C4 column for reversed-phase high-performance liquid chromatographic purification of synthetic oligonucleotides

  1985cited by this paper
• Reversed-phase high-performance liquid chromatographic separation of diastereomeric phosphorothioate analogues of oligodeoxyribonucleotides and other backbone-modified congeners of DNA.

  1985cited by this paper
• Reverse-phase polystyrene column for purification and analysis of DNA oligomers.

  1984cited by this paper
• The use of multidimensional chromatography for the isolation of synthetic oligodeoxyribonucleotides on a preparative scale

  1984cited by this paper
• Comparison of several high-performance liquid chromatography techniques for the separation of oligodeoxynucleotides according to their chain lengths

  1983cited by this paper
• Gradient elution in reversed-phase HPLC-separation of macromolecules

  1983cited by this paper
• High-performance liquid chromatography of the oligonucleotides

  1981cited by this paper
• Retention mechamism for reversed-phase ion-pair liquid chromatography

  1979cited by this paper
• High-pressure liquid chromatography in polynucleotide synthesis.

  1978cited by this paper

• Recent advances in the analysis of the evolutionary RNA based therapeutics; a review.

  2026cites this paper
• RNA sample preparation strategies for mass spectrometry sequencing of the epitranscriptome and therapeutic RNAs: A review.

  2026cites this paper
• Modern LC-MS strategies for comprehensive metabolite identification of therapeutic oligonucleotides.

  2026cites this paper
• The use of multiple liquid chromatography methods augmented by phosphorus-31 nuclear magnetic resonance to characterize the diastereomer composition in synthetic oligonucleotides

  2025cites this paper
• sgRNA Single-Nucleotide Resolution by Ion-Pairing Reversed-Phase Chromatography.

  2025cites this paper
• Considerations on advanced ion pairing co-gradients for ion-pair reversed phase liquid chromatography separations of oligonucleotides.

  2025cites this paper
• Direct RNA Modification Mapping: Technological Advances, Gaps, and Emerging Trends

  2025cites this paper
• Prediction of retention time in larger antisense oligonucleotide datasets using machine learning

  2025influential citation
• A platform method for simultaneous quantification of lipid and nucleic acid components in lipid nanoparticles.

  2025cites this paper
• A Roadmap guide on bioanalysis challenges and practical solutions for accurate quantification of oligonucleotide-based novel therapeutic modalities using LC-MS.

  2025cites this paper
• Enhanced Protein Precipitation with Ammonia Enables Rapid, Universal Extraction of Oligonucleotides for Bioanalysis

  2025cites this paper
• Impact of nucleotide hydrophobicity on oligonucleotides separation in liquid chromatography.

  2025cites this paper
• Separation of deaminated impurities from the desired oligonucleotides using supercritical fluid chromatography.

  2025cites this paper
• Mass photometry as a fast, facile characterization tool for direct measurement of mRNA length

  2025cites this paper
• Full comprehensive ion-pair-mediated anion exchange chromatography × ion-pair reversed-phase liquid chromatography fingerprinting of digested messenger ribonucleic acid drug substances

  2025cites this paper
• Current trends in liquid chromatography-mass spectrometry analysis of small interfering RNAs: A short review.

  2025cites this paper
• Online Nucleotide Mapping of mRNAs.

  2024cites this paper
• Ion-pair reversed-phase chromatography analysis of oligonucleotides using ultra-short (20 x 2.1 mm) columns. Tutorial

  2024cites this paper
• Stationary phase effects in hydrophilic interaction liquid chromatographic separation of oligonucleotides.

  2024cites this paper
• Understanding the fundamentals of the on-off retention mechanism of oligonucleotides and their application to high throughput analysis.

  2024cites this paper
• Weak to strong ion-pair gradients to expand the selectivity of oligonucleotide separations in reversed phase liquid chromatography - a proof of concept

  2024cites this paper
• Improvement of oligonucleotide separation using a repetto high-performance liquid chromatography recycling approach.

  2024cites this paper
• Impact of mobile and stationary phases on siRNA duplex stability in liquid chromatography.

  2024cites this paper
• Mixed-mode separation of antisense oligonucleotides using a single column with complementary anion-exchange and hydrophobic interaction chromatography approaches.

  2024cites this paper
• Mobile Phase Aging and Its Impact on Electrospray Ionization of Oligonucleotides

  2023cites this paper
• High-Throughput Chromatographic Separation of Oligonucleotides: A Proof of Concept Using Ultra-Short Columns

  2023cites this paper
• SCX-tip-aided LC-MS detection of active ricin via oligonucleotide substrates for depurination kinetics.

  2023cites this paper
• Characterization of antisense oligonucleotide and guide ribonucleic acid diastereomers by hydrophilic interaction liquid chromatography coupled to mass spectrometry.

  2023cites this paper
• Advances in nucleic acid sample preparation for electrospray ionization and matrix-assisted laser desorption ionization mass spectrometry

  2023cites this paper
• Polybutylene terephthalate-based stationary phase for ion-pair-free reversed-phase liquid chromatography of small interfering RNA. Part 1: Direct coupling with mass spectrometry.

  2023cites this paper
• Ion-pair reversed-phase and hydrophilic interaction chromatography methods for analysis of phosphorothioate oligonucleotides.

  2023cites this paper
• The Role of Separation Techniques in the Analysis of mRNA Therapeutic Drug Substances and Drug Products

  2023cites this paper
• Optimization of orthogonal separations for the analysis of oligonucleotides using 2D-LC.

  2023cites this paper
• Comprehensive evaluation of zwitterionic hydrophilic liquid chromatography stationary phases for oligonucleotide characterization.

  2023cites this paper
• Separation of therapeutic oligonucleotides using ion-pair reversed-phase chromatography based on fundamental separation science

  2023cites this paper
• Evaluating orthogonality between ion-pair reversed phase, anion exchange, and hydrophilic interaction liquid chromatography for the separation of synthetic oligonucleotides.

  2023cites this paper
• Polybutylene terephthalate-based stationary phase for ion-pair-free reversed-phase liquid chromatography of small interfering RNA. Part 2: Use for selective comprehensive two-dimensional liquid chromatography.

  2023cites this paper
• Detection of low-frequency mutations in clinical samples by increasing mutation abundance via the excision of wild-type sequences

  2023cites this paper
• Liquid Chromatography Methods for Analysis of mRNA Poly(A) Tail Length and Heterogeneity

  2023cites this paper
• LC–MS-Based Direct Quantification of MicroRNAs in Rat Blood

  2023cites this paper
• Development of an ion pairing reversed-phase liquid chromatography-mass spectrometry method for characterization of clustered regularly interspaced short palindromic repeats guide ribonucleic acid.

  2022cites this paper
• Prediction of peptides retention behavior in reversed‐phase liquid chromatography based on their hydrophobicity

  2022influential citation
• nSalt gradient and ion-pair mediated anion exchange of intact messenger ribonucleic acids

  2022cites this paper
• Assay, Purity, and Impurity Profile of Phosphorothioate Oligonucleotide Therapeutics by Ion Pair–HPLC–MS

  2022cites this paper
• Characterization of Impurities in Therapeutic RNAs at the Single Nucleotide Level.

  2022cites this paper
• Challenges and emerging trends in liquid chromatography-based analyses of mRNA pharmaceuticals

  2022cites this paper
• Effect of ion-pairing reagent hydrophobicity on liquid chromatography and mass spectrometry analysis of oligonucleotides.

  2022cites this paper
• The impact of low adsorption surfaces for the analysis of DNA and RNA oligonucleotides.

  2022cites this paper
• Phosphorothioate oligonucleotides separation in ion-pairing reversed-phase liquid chromatography: Effect of ion-pairing system.

  2022cites this paper
• Oligonucleotides Isolation and Separation—A Review on Adsorbent Selection

  2022influential citation
• Building machine-learning-based models for retention time and resolution predictions in ion pair chromatography of oligonucleotides.

  2022cites this paper
• Detection of fluorescence-labeled DNA with in-plane organic optoelectronic devices.

  2022cites this paper
• Evaluating the interplay among stationary phases/ion-pairing reagents/sequences for liquid chromatography mass spectrometry analysis of oligonucleotides.

  2021cites this paper
• Characterization and comparison of mixed-mode and reversed-phase columns; interaction abilities and applicability for peptide separation.

  2021cites this paper
• Impurity profiling of siRNA by two-dimensional liquid chromatography-mass spectrometry with quinine carbamate anion-exchanger and ion-pair reversed-phase chromatography.

  2021cites this paper
• Ion-pair Reversed-phase Liquid Chromatographic Separation of Oligonucleotides

  2021cites this paper
• Analytical techniques currently used in the pharmaceutical industry for the quality control of RNA-based therapeutics and ongoing developments.

  2021cites this paper
• Stringent Primer Termination by an Archaeo-Eukaryotic DNA Primase

  2021cites this paper
• Evaluation of Different Ion-Pairing Reagents for LC/UV and LC/MS Analysis of Oligonucleotides

  2021cites this paper
• Mass Spectrometry-Based Methods for Characterization of Hypomodifications in Transfer RNA

  2021cites this paper
• Purification of N-acetylgalactosamine-modified-oligonucleotides using orthogonal anion-exchange and mixed-mode chromatography approaches.

  2021cites this paper
• Selectivity limits of and opportunities for ion pair chromatographic separation of oligonucleotides.

  2021cites this paper
• Improved Chromatographic Analysis of Oligonucleotides with ACQUITY PREMIER Oligonucleotide BEH C 18 Columns

  2020cites this paper
• Development of liquid chromatography tandem mass spectrometry method to quantify cyclobutane pyrimidine dimer photolyase activity by detection of 15mer oligonucleotide as reaction product.

  2020cites this paper
• Oligonucleotides: Current trends and innovative applications in the synthesis, characterization and purification.

  2020cites this paper
• A signal transduction approach for multiplexed detection of transcription factors by integrating DNA nanotechnology, multi-channeled isothermal amplification, and chromatography.

  2020cites this paper
• The chromatographic behaviour of new double-labelled oligodeoxynucleotide probes containing azaphthalocyanine-dye as a quencher with respect to their purity evaluation.

  2020cites this paper
• Evaluation of alkylamines and stationary phases to improve LC-MS of oligonucleotides.

  2020cites this paper
• Characterization of therapeutic oligonucleotides by liquid chromatography.

  2020cites this paper
• Analysis of Antisense Oligonucleotides and Their Metabolites with the Use of Ion Pair Reversed-Phase Liquid Chromatography Coupled with Mass Spectrometry

  2019cites this paper
• Analysis of oligonucleotides by ion-pair reversed-phase liquid chromatography coupled with positive mode electrospray ionization mass spectrometry

  2019cites this paper
• Modeling Cationic Adduction of Oligonucleotides Using Electrospray Desorption Ionization.

  2019cites this paper
• Simultaneous separation of small interfering RNA and lipids using ion-pair reversed-phase liquid chromatography.

  2019cites this paper
• Effective Characterization of DNA Ligation Kinetics by High‐Resolution Melting Analysis

  2019cites this paper
• Analysis of the first and second generation of antisense oligonucleotides in serum samples with the use of ultra high performance liquid chromatography coupled with tandem mass spectrometry.

  2019cites this paper
• Oligonucleotide analysis by hydrophilic interaction liquid chromatography-mass spectrometry in the absence of ion-pair reagents.

  2019cites this paper
• A Small Helical Bundle Prepares Primer Synthesis by Binding Two Nucleotides that Enhance Sequence-Specific Recognition of the DNA Template.

  2019cites this paper
• Investigation of factors influencing the separation of diastereomers of phosphorothioated oligonucleotides

  2019cites this paper
• THERAPEUTIC OLIGONUCLEOTIDES, IMPURITIES, DEGRADANTS, AND THEIR CHARACTERIZATION BY MASS SPECTROMETRY.

  2019cites this paper
• Two-dimensional liquid chromatography coupled to mass spectrometry for impurity analysis of dye-conjugated oligonucleotides.

  2019cites this paper
• Analytical and preparative separation of phosphorothioated oligonucleotides: columns and ion-pair reagents

  2019cites this paper
• A review on native and denaturing purification methods for non-coding RNA (ncRNA).

  2019cites this paper
• Rapid oligonucleotide drug impurity determination by direct spectral comparison of ion-pair reversed-phase high-performance liquid chromatography electrospray ionization mass spectrometry data

  2018cites this paper
• Rapid Oligonucleotide Drug Impurity Determination by Direct Spectral Comparison of Ion Pair-Reversed Phase HPLC ESI MS Data.

  2018cites this paper
• The anticancer activity of antisense microRNA (fRNA) in combination with the lectin from Bacillus subtilis B‐7025

  2018cites this paper
• Alkylamine ion-pairing reagents and the chromatographic separation of oligonucleotides.

  2018cites this paper
• Monolithic alkylsilane column: A promising separation medium for oligonucleotides by ion-pair reversed-phase liquid chromatography.

  2018cites this paper
• Two-dimensional liquid chromatography-mass spectrometry for the characterization of modified oligonucleotide impurities.

  2018cites this paper
• A novel strategy for retention prediction of nucleic acids with their sequence information in ion-pair reversed phase liquid chromatography.

  2018cites this paper
• Study on the polymorphism of G-quadruplexes by reversed-phase HPLC and LC-MS.

  2018cites this paper
• Chromatographic approaches for the characterization and quality control of therapeutic oligonucleotide impurities.

  2018cites this paper
• Review on investigations of antisense oligonucleotides with the use of mass spectrometry.

  2018cites this paper
• Offline comprehensive liquid chromatography in combination with a 1 Deoxyribonuclease I immobilized enzymatic reactor for selective screening of 2 oligonucleotide mixtures 3

  2018cites this paper
• Chemically-enzymatic synthesis of photosensitive DNA.

  2017cites this paper
• Analysis of oligonucleotides by ion-pairing hydrophilic interaction liquid chromatography/electrospray ionization mass spectrometry.

  2017cites this paper
• Assessing the Interplay between the Physicochemical Parameters of Ion-Pairing Reagents and the Analyte Sequence on the Electrospray Desorption Process for Oligonucleotides

  2017cites this paper
• Liquid chromatographic separation of oligonucleotides

  2017cites this paper
• The anticancer activity and immune modulating properties of combinatorial ensemble complementary (antisense) microRNA (fRNA) in combination with the immunomodulator - glycoproteid lectin from B. subtilis B-7025

  2017cites this paper
• Comprehensive chlorophyll composition in the main edible seaweeds.

  2017cites this paper
• Characterization of Apolipoprotein C3 (Apo C3) LNA/DNA Impurities and Degradation Products by LC-MS/MS

  2017cites this paper